Aizhen Guo

Jilin University, Jilin, Jilin Sheng, China

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Publications (3)4.3 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: Tuberculosis (TB) is still one of the most common causes of death in the world. The emergence of multidrug-resistant and extensively drug-resistant (XDR-TB) Mycobacterium tuberculosis (M. tuberculosis) strains has increased the importance of searching for alternative targets to develop new antimycobacterial drugs. Linezolid, the first of oxazolidinones, is active in vitro against M. tuberculosis, but the response mechanisms of M. tuberculosis to linezolid are still poorly understood. To reveal the possible mechanism of action of linezolid against M. tuberculosis, commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes triggered by treatment with subinhibitory concentrations of linezolid. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. A total of 729 genes were found to be differentially regulated by linezolid. Among these, 318 genes were upregulated, and 411 genes were downregulated. A number of important genes were significantly regulated that are involved in various pathways, such as protein synthesis, sulfite metabolism, and genes involved in the cell envelope and virulence. This genome-wide transcriptomics approach produced the first insights into the response of M. tuberculosis to a linezolid challenge.
    Current Microbiology 03/2012; 64(6):530-8. · 1.52 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: Chelerythrine (a natural quaternary benzophenanthridine alkaloid) is an extract from the roots of Chelidonium majus with potential antimycobacterial activity. To reveal the possible mechanism of action of chelerythrine against Mycobacterium tuberculosis (M. tuberculosis), commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes triggered by treatment with subinhibitory concentrations of chelerythrine. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data using Agilent software. Analysis of the microarray data revealed that a total of 759 genes were differentially regulated by chelerythrine. Of these, 372 genes were upregulated, and 387 genes were downregulated. Some of the important genes that were significantly regulated are related to different pathways (such as urease), methoxy-mycolic acid synthase, surface-exposed lipids, the heat shock response, and protein synthesis. This genome-wide transcriptomics approach produced the first insights into the response of M. tuberculosis to a chelerythrine challenge.
    Current Microbiology 04/2011; 62(4):1200-8. · 1.52 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: Mycobacterium tuberculosis (M. tuberculosis) remains one of the world’s most prevalent and serious pathogens, and multiple resistant strains are increasingly widespread. The recent emergence of multidrug-resistant and extensively drug-resistant (XDR-TB) strains has increased the importance of searching for alternative targets to develop new antimycobacterial drugs. Plumbagin (PL), a natural plant product, has been reported to have potential antimicrobial activity against M. tuberculosis, but the response mechanisms of M. tuberculosis to PL are still poorly understood. In this study, we used a commercial oligonucleotide microarray to determine the overall transcriptional response of M. tuberculosis H37Rv triggered by exposure to subinhibitory concentrations of PL. A total of 274 genes were found to be differentially regulated by PL. Of these, 103 genes were upregulated, and 171 genes were downregulated. A number of important genes were significantly regulated that are involved in various pathways, such as chaperonins, PE/PPE multigene families, and genes involved in the cell envelope, virulence, and 50S ribosomes. To our knowledge, this genome-wide transcriptomics approach has produced the first insights into the response of M. tuberculosis to a PL challenge. These results will pave the way for exploring the mechanisms of PL action against M. tuberculosis. KeywordsAntimycobacterial–Plumbagin– Mycobacterium tuberculosis –Microarray
    World Journal of Microbiology and Biotechnology 01/2011; 27(10):2261-2269. · 1.26 Impact Factor

Publication Stats

3 Citations
265 Views
4.30 Total Impact Points

Institutions

  • 2011–2012
    • Jilin University
      • College of Animal Science and Veterinary Medicine
      Jilin, Jilin Sheng, China
    • Huazhong Agricultural University
      • College of Veterinary Medicine
      Wu-han-shih, Hubei, China