Publications (2)1.29 Total impact
Article: The association between infection burden in Iranian patients with acute myocardial infarction and unstable angina.[show abstract] [hide abstract]
ABSTRACT: to evaluate the association of ischemic heart disease (IHD) with the number of pathogens (infection burden) among individuals with infection. a total of 120 patients with IHD as the acute myocardial infarction (AMI; n=60) or unstable angina (UA; n=60) group and 60 healthy subjects with sex- and age-matched as control group were enrolled in this study. Serum samples of all participants were tested for the presence of antibodies to Helicobacter pylori (H. pylori), cytomegalovirus (CMV), type-1 herpes simplex virus (HSV-1) and type- 2 HSV (HSV-2) by using ELISA. Regarding the association of the infection burden with IHD, the prevalence ratios and 95% confidence intervals (CI) were 3.18 (CI: 1.50-6.72; P<0.001) for 3 seropositivities and 3.83 (CI: 0.84-17.43; P<0.05) for 4 seropositivities. The rate of subjects with high infection burden (3 seropositivities) was significantly higher in IHD group as compared to control group (53.4% vs 21.6%; P<0.01). Moreover, the mean number of seropositivities was also significantly higher in patients with IHD in comparison to control group (2.47 vs 1.68; P<0.01). The seroprevalence of anti-H. pylori antibodies in AMI and UA groups was significantly higher compared to control group (P<0.0001). The seroprevalence of anti-CMV antibodies in AMI and UA group was also significantly higher than those observed in control group (P<0.01). Moreover, the seroprevalence of anti-HSV-1 antibodies was significantly higher in AMI and UA groups in comparison to control group (P<0.001). The seroprevalence of anti-HSV-2 antibodies was similarly expressed in patients and healthy control group. the infection burden was significantly higher in patients with IHD, which represent that the parameter should also be considered as an independent risk factor for development of IHD. The seroprevalence of H. pylori, CMV and HSV-1 were also higher in patients with IHD.Acta medica Indonesiana 04/2011; 43(2):105-11.
Article: Polymorphisms within Exon 9 but not intron 8 of the Vitamin D Receptor are associated with the nephropathic complication of type-2 diabetes[show abstract] [hide abstract]
ABSTRACT: Background: The impact of several environmental and genetic factors on diabetes and its complications is well documented but there is an urgent need to understand more about genetic risk factors associated with this disease. The present study was aimed at examining the two single nucleotide polymorphisms (SNP) in intron 8 and exon 9 of the vitamin D receptor (VDR) gene in nephropathic and non-nephropathic type-2 diabetic patients. Material and methods: In this clinical study, peripheral blood samples were obtained from 100 type-2 diabetic patients, 100 nephropathic type-2 diabetic patients and 100 healthy controls. DNA was extracted and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to examine two SNP polymorphisms within the VDR gene. Results: Our results showed a significant difference in the Taq-1 evaluated genotypes of exon 9 within the VDR gene of diabetic individuals with (p=0.012) and without (p<0.001) nephropathy. Analysis of the Taq-1 evaluated alleles of nephropathic (p=0.917) and none-nephropathic (p=1.000) did not show a significant difference. We also evaluated the intron 8 Apa-1 alleles in patients with (p=0.480) and without nephropathy (p=0.543) and determined there were no differences between these groups. Our results also showed that the frequency of Apa-1 genotypes did not differ in nephropathic (p=0.224) and none-nephropathic (p=0.236) diabetic patients. Conclusion: Based on our results, it can be concluded that VDR and its functional polymorphism in exon 9 may play an important role in pathogenesis of type-2 diabetes and more investigations are required to clarify their role in nephropathy. Yes YesInternational Journal of Immunogenetics 12/2010; · 1.29 Impact Factor