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ABSTRACT: Twelve monoclonal antibodies (mAbs) formed temporary cluster TC36. mAb CACTB6A was included as a standard antibody that reacted with the gamma/delta T cell receptor (TCR). Eight of the mAbs, CACTB6A, CACT16A, CACT17A, CACTB12A, CACTB44A, CACT71A, CACT18A and CACT61A, immunoprecipitated a 47 kDa molecule indicating that they all recognize the gamma/delta TCR. Flow cytometric studies separated these mAbs into three groups that distinguish three different gamma/delta TCR. mAb BAQ72A, which fell within TC36, immunoprecipitated a 215 kDa molecule with a similar tissue and cellular distribution to BoWC1 mAb. mAbs CACT26A, CACT63A and CACT77A reacted similarly to each other by flow cytometry and immunohistology, but were distinct from the other mAbs in TC36. These three mAbs may recognize an activation molecule and form a distinct novel mAb cluster.
Veterinary Immunology and Immunopathology 12/1993; 39(1-3):161-7. · 2.08 Impact Factor
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ABSTRACT: The nine antibodies (mAbs) which clustered with the standard WC1 mAb CC15 in temporary cluster TC22 were analysed by immunoprecipitation, flow cytometry and immunohistology. Two forms of the molecule, with molecular weights of 205 and 215 kDa, were identified by immunoprecipitation and SDS-PAGE, the expression of which correlated with subclusters generated by flow cytometric analysis. Immunohistological data, however, did not reveal any tissue specific distribution. We propose the existence of at least two isoforms of WC1 which may prove to identify subpopulations of CD2- CD4- CD8- gamma/delta T cells.
Veterinary Immunology and Immunopathology 12/1993; 39(1-3):137-44. · 2.08 Impact Factor
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ABSTRACT: The nine antibodies (mAbs) which clustered with the standard WC1 mAb CC15 in temporary cluster TC22 were analysed by immunoprecipitation, flow cytometry and immunohistology. Two forms of the molecule, with molecular weights of 205 and 215 kDa, were identified by immunoprecipitation and SDS-PAGE, the expression of which correlated with subclusters generated by flow cytometric analysis. Immunohistological data, however, did not reveal any tissue specific distribution.We propose the existence of at least two isoforms of WC1 which may prove to identify subpopulations of CD2− CD4− CD8− γ/δ T cells.
Veterinary Immunology and Immunopathology 39:137-144. · 2.08 Impact Factor
