Zhongguo fei ai za zhi = Chinese journal of lung cancer 02/2013; 16(2):107-13.
Zhongguo fei ai za zhi = Chinese journal of lung cancer 07/2012; 15(7):439-45.
ABSTRACT: ObjectiveLung carcinoma with spindle and (or) giant cell (LCSG) is a rare epithelial malignant tumor. The aim of our study is to investigate
the clinicopathological and prognostic characteristics of 17 cases of LCSGs.
MethodsAmong 421 patients underwent resection of lung carcinomas, 17 cases of LCSG were studied for clinical, gross and histological
parameters. Follow-up information was obtained and analyzed to clarify prognostically significant parameters.
ResultsThe LCSG patients consisted of 15 males and 2 females, with the age ranging from 45 to 78 years (median, 58 years); 5 cases
of stage I, 3 of stage II, 9 of stage III by pathological TNM staging; 2 cases of exclusively spindle cell carcinoma, 5 cases
of lung carcinoma with spindle cell, 10 cases of lung carcinoma with giant-cell carcinoma. Cough, chest distress, or chest
pain were the most common presenting symptoms, occurring in 15 patients (88.2%). Of 5 patients in stage I, 4 were alive and
free of relapse for more than 5 years. The difference in survival was statistically significant between LCSG and squamous
cell carcinoma patients (median survival, 36 vs. 61 months; P = 0.027). Lymph node metastasis and carcinoma with giant cell were the hazardous factors impacting postoperative prognosis
of LCSG patients.
ConclusionLCSG patients in early stage may have an optimistic outcome. Lung carcinomas with giant cell displayed multiple cell components
in histopathology, and poor outcome due to more lymph node involved.
The Chinese-German Journal of Clinical Oncology 04/2012; 8(1):1-6.
Zhongguo fei ai za zhi = Chinese journal of lung cancer 10/2011; 14(10):819-24.
Zhongguo fei ai za zhi = Chinese journal of lung cancer 01/2009; 12(1):73-7.
ABSTRACT: Formalin-fixed and paraffin-embedded tissue (FPE) with long term follow-up and clinicopathological data are extensively available and easily accessible. They represent an extensive source of genetic materials and proteins to be investigated for clinical usage. We conducted the study to explore the possibility of extracting RNA from FPE of lung cancer, and checking up the transcription levels of the promyelocytic leukemia gene (PML) by RT-PCR.
five fresh frozen lung adenocarcinoma tissues and 40 FPEs of lung cancer were chosen; FPEs had been conserved for 60-85 months. All samples had been identified lacking expression of PML protein by immunohistochemical staining. The total RNA was extracted by using Trizol one-step method, and verified by testing OD value. RT-PCR was used to detect the transcription level of PML gene.
The RNAs from both tissues had the OD value ranging from 1.7 to 2.1. RT-PCR results displayed that mRNA of PML (295 bps) and beta-actin (318 bps) was not absent in all cancer tissues.
Trizol one-step method is easily accessible, and reliable for extracting RNA in fragments around 300 bases from FPEs. Loss of PML protein in lung cancer is not caused by downward adjustment of mRNA.
Zhongguo fei ai za zhi = Chinese journal of lung cancer 08/2008; 11(4):563-6.
ABSTRACT: The promyelocytic leukaemia (PML) protein has been implicated in control of key tumor-suppressive pathways. However, its role in pathogenesis of lung cancer is still unclear. The objective of this study is to assess expression and clinical significance of PML, P53 and P16INK4A in lung cancer, as well as the relation of these factors.
The tissue microarrays were created with samples from lung cancers (n=148), pulmonary benign lung tumors (n=5) and normal lung tissues (n=7), and protein expression was analyzed by immunohistochemical staining. The association between protein expression and clinical parameters was evaluated by using Crosstabs method. The Kaplan-Meier method was used to estimate survival. Cox proportional hazards model was used for univariate and multivariate analysis.
There was at least triplicate 0.6-mm cores per sample, 4 cases of lung cancer were excluded for lacking of enough tissue. PML was found in the cytoplasm of 14.0% cases of NSCLC and of 39.1% SCLC (P=0.010), and in the nuclei of 31.4% NSCLC and 8.7% SCLC respectively (P=0.026). PML protein was present in 9 patients with SCLC and absent in 14 cases, 5-year cumulative survival rate of the patients was 50% and 23% respectively (Log-rank test, P=0.047). Lacking of PML protein and senior pathologic T-stage were two hazardous factors that influenced prognosis of SCLC. P53 expression was found in 33.3% lung cancer, and absent in benign tumors and normal tissues of the lung (P=0.038). P16INK4A expression was abolished in normal lung tissue, however, increased in lung cancer (28.5%), and especially in lung cancer with non- or poor differentiation (36.5%) and in SCLC (69.6%). There was inverse correlation between PML expression in the nuclei and P16INK4A expression, positive correlation between P53 and P16INK4A expressions in lung cancers. PML was negatively correlated with P53 in squamous cell carcinoma.
As an important suppressor of tumor, PML is related with P53 mutation in squamous cell carcinoma. Increased P16INK4A protein in lung cancer may be the results of gene mutation, and be related with mutant P53 protein.
Zhongguo fei ai za zhi = Chinese journal of lung cancer 06/2007; 10(3):176-82.
ABSTRACT: Tissue microarray provides a convenient shortcut for immunohistochemical staining. The aim of this study is to investigate the clinicopathologic and prognostic values of survivin and cyclooxygenase-2 (COX-2) expression level in patients with non-small cell lung cancer (NSCLC).
Expression of survivin and COX-2 was detected in 88 cases of NSCLC and 5 cases of normal lung samples by immunohistochemical staining on tissue microarray sections. All cases were followed up for more than 5 years.
Cytoplasmic and nuclear expression rate of survivin in NSCLC was 94.3% and 79.5%, respectively, and positive expression rate of COX-2 was 71.6%, however, neither survivin nor COX-2 expression was observed in normal lung tissues (P < 0.005). Nuclear expression of survivin was markedly higher in smokers than that in non-smokers (P=0.002). The positive expression of COX-2 was significantly related to gender, smoking, histologic subtype and lymph node metastasis (P < 0.05). Univariate analysis showed that patients with positive expression of COX-2 had worse overall survival (P=0.014), however, survivin expression was not related to survival. Multivariate analysis showed that neither survivin nor COX-2 was independent prognostic factor for survival.
The results indicate that survivin highly expresses in NSCLC, so the ubiquitous expression makes it a potential novel parameter for diagnosis of NSCLC. Aberrant expression of COX-2 is related to worse overall survival, which may be useful to predict prognosis for NSCLC.
Zhongguo fei ai za zhi = Chinese journal of lung cancer 04/2007; 10(2):133-7.