Are you Yoshiaki Ohnishi?

Claim your profile

Publications (13)15.03 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Focal segmental glomerulosclerosis (FSGS) often leads to refractory nephrotic syndrome (NS). A high level of low-density lipoprotein (LDL) is a risk factor for the progression of NS. An 8-year-old girl presented with severe proteinuria refractory to steroid therapy. She was diagnosed with non-IgA diffuse mesangial proliferative glomerulonephritis. Oral prednisolone, methylprednisolone (mPL) pulse therapy, and cyclosporine and cyclophosphamide therapy failed to achieve remission. Follow-up renal biopsy revealed FSGS. Her serum level of LDL was high, and LDL-apheresis (LDL-A) was performed five times, followed by mPL pulse therapy. Urinary protein decreased from 2-4 g x day(-) to 0.5-1.0 g x day(-). LDL-A may be beneficial in the treatment of multidrug-resistant FSGS.
    Journal of Anesthesia 02/2009; 23(2):284-7. · 0.87 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Continuous hemodiafiltration (CHDF) has been performed for the treatment of severe acute pancreatitis. Phospholipase A2 (PLA2) is one of the important mediators which exacerbate acute pancreatitis, but whether PLA2 can be removed by CHDF is unclear. In this study, the kinetics of group IB and group IIA PLA2 was examined at the first session of low-volume CHDF in eight patients with severe acute pancreatitis. CHDF was performed using polysulfone hemofilters (surface area: 0.7 m(2)) at a blood flow rate of 100 mL/min and a filtration and dialysate flow rate of 10 mL/min each. The plasma concentrations of group IB and IIA PLA2 before the start of CHDF were 47.4 +/- 52.0 microg/L and 352 +/- 390 microg/L, respectively, and did not change significantly. The clearances of group IB and IIA PLA2 achieved by the CHDF circuit 1 h after the start of CHDF were 20.7 +/- 11.6 mL/min and 16.7 +/- 4.4 mL/min, respectively, with both clearances decreasing significantly with time. The clearance of group IB PLA2 into the waste fluid tended to increase with time; however, the concentrations of group IIA PLA2 in the waste fluid were less than the measurable sensitivity. These results indicate that group IB PLA2 is adsorbed on the hemofilter membrane in preference to being removed into the waste fluid, while group IIA PLA2 is mainly removed by adsorption. However, low-volume CHDF is not effective at eliminating the group IB and IIA PLA2 plasma concentration.
    Artificial Organs 06/2007; 31(5):395-401. · 1.96 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In Japan, leukocytapheresis (LCAP) therapy has been carried out for ulcerative colitis as an effective therapy with a low incidence of side-effects. In the present study, we serially investigated the influence of LCAP therapy on anemia and hemodynamics in patients with ulcerative colitis using a non-invasive method with a Crit-Line monitor (CLM) (Hema Metrics Inc., Salt Lake City, UT and Boston, MA, USA). We carried out LCAP on 10 patients with ulcerative colitis using a CS-100 Cellsorba EX LCAP filter in 34 courses of LCAP. The mean hematocrit value, which was measured using the Crit-Line monitor, was 21.8 +/- 0.2% (21.0-22.4%), which showed no significant changes after each LCAP therapy. The actual erythrocyte count, hemoglobin and hematocrit values decreased by 244 000/mm3, 0.7 g/dL and 2.1%, respectively, however, the differences were insignificant. These values corresponded to 7.8, 8.0 and 7.6% of the values before LCAP, respectively. There were no significant changes in systolic blood pressure, diastolic blood pressure, or heart rate during LCAP. The results of this study suggest that one course of LCAP does not exacerbate anemia, and it does not influence hemodynamics. However, considering the exacerbation of anemia in patients with severe ulcerative colitis caused by massive melena, monitoring with a less invasive Crit-Line monitor and a vital information monitor might be useful.
    Therapeutic apheresis and dialysis: official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy 03/2007; 11(1):16-21. · 1.53 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: There is a disorder that is called Guillain-Barre Symdrome(GBS). Currently, plasmapheresis and high-dose immunoglobulin therapy are used for GBS. Plasmapheresis is one of the blood purifications. When the blood purification is enforced, it should be careful to decrease the blood pressure. In the Tokushima University Hospital, the hematocrit(Ht) values are measured using Crit-Line Monitor(CLM), because the Ht values are rate of red cell in blood, and are relation of the blood pressure. Hence, it is important to predict Ht value using the prediction method. There are various prediction methods, but we proposed the new prediction method. The purpose of this study is the prediction of Ht value after 1,3, and 5 minutes during the plasma exchange using the new prediction method. As the results, rms errors were small in all prediction, but the predicted Ht values are same the measured Ht value before 1,3, and 5 minutes, because the measured Ht values are strong autocorrelation.
    Conference proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 02/2007; 2007:3160-3.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Continuous monitoring of hematocrit with a CRIT-LINE monitor (CLM) is used to prevent excess ultrafiltration during hemodialysis and continuous renal replacement therapy. The presence of substances affecting the scattering and absorption rates of multiple wavelengths of near infrared rays of CLM in the blood may affect the measured values with CLM. We examined the influences of lipid emulsion (LE) on hematocrit and relative blood volume (RBV) which were measured with CLM using an in vitro experimental model with human blood. Additions of 10% or 20% of LE increased the hematocrit measured by LCM and decreased the percent change of RBV in proportion to the dose. One percentage of 20% LE in the plasma increased the expected hematocrit measured with CLM by 2.9%. The decrease of initial hematocrit from 48.1% to 43.4% decreased the expected percent change of RBV from -3.4% to -3.7% with the addition of 1 ml of 20% LE to 100 ml blood. These findings indicate that additions of LE increase hematocrit that is measured with CLM in proportion to the dose of LE. Low levels of initial hematocrit will increase the degree of expected percent change of RBV. Attention should be paid to the influence of LE during monitoring with CLM.
    ASAIO journal (American Society for Artificial Internal Organs: 1992) 01/2007; 53(4):474-8. · 1.39 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Continuous hemodiafiltration (CHDF) has recently been used for treatment of severe acute pancreatitis. CHDF is capable of eliminating small molecules from blood, but whether trypsin can be eliminated by CHDF is not clear. In this study, elimination of trypsin-like enzyme activity (TLE) and cationic trypsin-like immunoreactivity (TLI) using low-volume CHDF was examined at the first CHDF session in eight patients with severe acute pancreatitis. CHDF was performed with a polysulfone hemofilter (membrane area, 0.7 m2) and nafamostat mesilate, a protease inhibitor and anticoagulant, at a blood flow rate of 100 ml/min and a filtration and dialysis flow rate of 10 ml/min each. Before beginning CHDF, plasma TLE was 3.41 +/- 2.86 nmol/(ml.min), and TLI was 5,900 +/- 9,008 ng/ml. The average plasma clearances of TLE and TLI achieved by the circuit during the 12-hour therapy were 56.7 +/- 4.9 ml/min and 8.0 +/- 7.2 ml/min, respectively. The average plasma clearance of TLI into the waste fluid was 2.4 +/- 1.6 ml/min whereas TLE was below the measurable sensitivity. The plasma concentration of TLE and TLI remained unchanged. These results indicate that low-volume CHDF using nafamostat mesilate as an anticoagulant can increase trypsin plasma clearance. However, low-volume CHDF is not effective to eliminate the plasma trypsin concentration.
    ASAIO journal (American Society for Artificial Internal Organs: 1992) 01/2007; 53(2):207-12. · 1.39 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A clipped single-bag for bicarbonate replacement fluid was developed to ensure proper mixing before administering to the patient. Nonmixture can cause imbalances of electrolytes and pH, which is a key problem for the current double-bag type bicarbonate replacement fluid sets. To resolve this problem, this single bag properly mixes the solutions before use. The new bag consists of a clip that is placed in the middle to keep the two solutions separated and sealed. When the caregiver is ready to administer treatment, the bag is simply unfolded and the clip automatically detaches, releasing the fluids. Thereby, the bicarbonate fluids are effectively mixed. An optimal clip size with an outer diameter of 16 mm and thickness of 2 mm was determined using compression tests and drop tests. This bag may be a safer and more effective way to provide proper replacement fluid supply for both hemofiltration and hemodiafiltration.
    ASAIO Journal 01/2006; 52(3):343-8. · 1.49 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A web-based monitoring system for the alarm of equipment has developed for the conventional environment of Intensive Care Unit (ICU). The system communicates with equipment using Data Collection Interface (DCI) that converts the protocol of the output of equipment from RS-232C to TCP/IP. The system creates a web-document that can be referred from any internet-connected personal computer in the hospital. Using the system, a staff can easily monitor the state of the patient and the equipment. If the system is installed in the ICU, monitoring and management for the equipment will be highly improved.
    Conference proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 02/2005; 6:6029-32.
  • Asaio Journal - ASAIO J. 01/2005; 51(2).
  • [Show abstract] [Hide abstract]
    ABSTRACT: Since plasma exchange (PE) and intravenous immunoglobulin (i.v.Ig) have been widely used in treatment for Guillain-Barré syndrome (GBS), early relapse and treatment-related fluctuation have been a potential problem, but little is known about the mechanism of relapse and fluctuation. We describe a patient who had GBS with treatment-related fluctuation. A 37-year-old Japanese man exhibited acute distal-dominant weakness in upper limbs after upper respiratory infection. His cranial nerve system was normal and muscle weakness was limited to upper limbs. Anti-GT1a IgG was strongly positive and anti-GQ1b IgG was also detected in his serum. Muscle weakness responded well to double-filtration plasmapheresis (DFPP) followed by i.v.Ig, but relapsed 45 days after the initial treatment. Although repeated treatments were effective, the patient showed additional minor deterioration twice. Motor nerve conduction velocities (MCVs) corresponded to the muscle weakness, but elevated level of cerebrospinal fluid (CSF) protein remained and anti-ganglioside antibody titers steadily decreased throughout the clinical course. These findings indicate that the clinical fluctuation was not due to changes in the production of anti-ganglioside antibodies but presumably to the transient beneficial effects of DFPP/i.v.Ig and the outlasting inflammatory response in peripheral nerves.
    Journal of the Neurological Sciences 07/2003; 210(1-2):105-8. · 2.24 Impact Factor
  • European Journal of Neurology 12/2002; 9(6):697-8. · 4.16 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: There are various blood purifications. Plasma Exchange(PE) is one of them. It is very important for medical workers to classify the changing Hematocrit(Ht) values during PE. Ht values have been obtained by CLIT-LINE monitor. Ht values mean the circulating blood volume. At present, the medical worker classifies the changing Ht values during the PE. The classification is depended on their experiment. Therefore, the classification is subjective. If we can find the information of the changing Ht values using the AR model, and can predict the changing Ht values, PE is treated more safety. Hence, our purpose is the classification into the rest of Ht values using the Ht values for less than 20 minutes from the beginning of PE by AR model. Initially, we have classified the Ht data into 3 groups. The 3 groups are falling, constant, and rising Ht values. The coefficients of ARmodel have updated by the delta rule using the Ht values of less than 20 minutes from the beginning of PE. AR model has captured the character of Ht values for less than 20 minutes from the beginning of PE, and rest of Ht values have been classified by the coefficients about the AR model. As the result, it is difficult to classify the rest of Ht value using this method. However, we have found that Ht values for less than 20 minutes from the beginning of PE must include some information about the rest of Ht values.
  • [Show abstract] [Hide abstract]
    ABSTRACT: There are various blood purifications. Plasma Exchange(PE) is one of the blood purifications. A patient’s circulating blood volume is changing during the PE. Hence, the medical workers must treat to stabilize the circulating blood volume. They are the CLIT-LINE monitor (CLM) to obtain the hematocrit (Ht) values, because the circulating blood volume is calculated by Ht values. Ht value reflects the count of red cells in the circulating blood volume, and Ht values are changed by the urination. They recognize the patient’s condition by the classifying the change of Ht values. However, the classifying the change of Ht values is depended on the experience of medical workers. This approach is very subjective. Hence, our purpose is the investigation of the possibility about the classifying the change of Ht value using the Self Organized feature Map (SOM). We used the Ht value that was obtained in the Tokushima University Hospital. The Ht data was greatly changed by urination. At first, the Ht data is divided into the consecutive grouped patterns. We used recurrent neural network (RNN) to treat the change of Ht value in each group. RNN is effective to treat the feature of time series signals, and it was known that the weight values of RNN have some information about the feature of signals. The seconds, the weight values obtained in each group are used as input data to SOM. As a result of SOM, Ht data were divided into 4 groups. One of them was a group into which Ht values were greatly changed by urination. It is thought that other groups show the state that has changed little by little during the PE. As a conclusion, it was supposed that the possibility about the classifying the change of Ht value using SOM.