[show abstract][hide abstract] ABSTRACT: To investigate the non-Helicobacter pylori (H. pylori) bacterial flora concurrent with H. pylori infection.
A total of 103 gastric biopsy specimens from H. pylori positive patients were selected for bacterial culture. All the non-H. pylori bacterial isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
A total of 201 non-H. pylori bacterial isolates were cultivated from 67 (65.0%) of the 103 gastric samples, including 153 isolates identified successfully at species level and 48 at genus level by MALDI-TOF MS. The dominant species were Streptococcus, Neisseria, Rothia and Staphylococcus, which differed from the predominantly acid resistant species reported previously in healthy volunteers. The prevalence of non-H. pylori bacteria was higher in non-ulcer dyspepsia group than in gastric ulcer group (100% vs 42.9%, P < 0.001). Six bacterial species with urease activity (Staphylococcus epidermidis, Staphylococcus warneri, Staphylococcus capitis, Staphylococcus aureus, Brevibacterium spp. and Klebsiella pneumoniae) were also isolated.
There is a high prevalence of the non-H. pylori bacteria concurrent with H. pylori infection, and the non-H. pylori bacteria may also play important as-yet-undiscovered roles in the pathogenesis of stomach disorders.
World Journal of Gastroenterology 03/2012; 18(11):1257-61. · 2.55 Impact Factor
[show abstract][hide abstract] ABSTRACT: During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China. With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed.
Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3-10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry.
502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426, with lower express volumes too. No Opa and Opc were detected in 053426.
The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.
Biomedical and Environmental Sciences 08/2010; 23(4):251-8. · 1.15 Impact Factor
[show abstract][hide abstract] ABSTRACT: In this paper, we propose a novel fusion-based gender classification method for 3D frontal neutral expression facial shape. Face landmarks, extracted from 3D face shape based on profiles and curvature, are separated as four regions. Experimental investigation to evaluate the significance of different facial regions in the task of gender classification is performed. The classification is performed by using Support Vector Machines (SVMs) based on the feature of regions. Classification results show that the upper region of face contains the highest amount of gender information. Matcher weighting fusion method is also applied to fusion the classification result of four regions. Experimental results demonstrate that fusing multiple facial features can achieve highest correct classification rate to 94.3%.
Computer and Automation Engineering (ICCAE), 2010 The 2nd International Conference on; 03/2010
[show abstract][hide abstract] ABSTRACT: This paper presents a novel facial localization method for 3D face in the presence of facial pose and expression variation. An idea of using Multi-level Partition of Unity (MPU) Implicits in a hierarchical way is proposed for reconstruction of face surface. Based on the analysis of curvature features, nose and eyeholes regions can be detected on lower level reconstructed face surface uniquely. Experimental results show that this method is invariant to pose, holes, noise and expression. The overall performance of 99.18% is achieved.
[show abstract][hide abstract] ABSTRACT: To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane.
Blood samples were collected from 14 volunteers (8 positive and 6 negative for H pylori detected by (13)C-urea breath test) of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum. The proteins related to the positive bands were identified by mass spectrum analysis.
Anti-H pylori antibodies had cross-reaction with the proteins of about 50 kDa of erythrocyte membranes in all samples independent of H pylori infection. One protein in the positive band was identified as Chain S, the crystal structure of the cytoplasmic domain of human erythrocyte Band-3 protein.
Anti-H pylori antibodies cross-react with some antigens of human erythrocyte membrane, which may provide a clue for the relationship between H pylori infection and vascular disorders.
World Journal of Gastroenterology 08/2007; 13(27):3742-6. · 2.55 Impact Factor
[show abstract][hide abstract] ABSTRACT: Specific immunoglobulin G antibody for severe acute respiratory syndrome (SARS) coronavirus was detected in maternal blood, umbilical blood, and amniotic fluid from a pregnant SARS patient. Potential protection of fetus from infection was suggested.
[show abstract][hide abstract] ABSTRACT: The strains of Yersinia pestis isolated in different period and different natural foci in China were analyzed.
Traditional and molecular biological methods were used. Rhamnose fermentation, rRNA gene copy number, nitrite reduction, and the glycerol fermentation were important characters for typing, and pulse field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) profile could reflect the genetic distance between the strains.
The strains could be divided into 15 genetic types by those 6 characters with each of them covered an isolated geographical territories.
The characters of strains were described; the genetic relationship of different types, their evolution, and the forming and shift of plague natural foci were analyzed.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 12/2003; 24(11):1005-9.