[Show abstract][Hide abstract] ABSTRACT: Abstract During screening of Korean indigenous medicinal herbs with anti-neurodegenerative activities, we found that the extract of Acanthopanax divaricatus vat. albeofructus could inhibit rotenone-induced DNA and cell damage in neuroblastoma cells. A simple in vitro model developed for the study of possible mechanisms underlying neurodegeneration in Parkinson’s disease includes the administration of rotenone to the human dopaminergic neuroblastoma cell line, SH-SY5Y. In this investigation, rotenone induced oxidative DNA damage of lymphocytes as expected. However, the oxidative DNA damage was inhibited in vitro upon treating Acanthopanax extract. Moreover, Acanthopanax extract resulted in the inhibitory activities against cell damage, ROS generation and chromatin condensation by rotenone.
Toxicology and Environmental Health Sciences. 12/2013; 4(4).
[Show abstract][Hide abstract] ABSTRACT: The aim of the study was to examine the liver tissue damage induced by nanosized-TiO2 in mouse. The biochemical parameters of liver, namely glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase and alkaline phosphatase were enhanced approximately 18%, 35% and 69% by exposure to nanosized-TiO2, respectively. The nanosized-TiO2 accumulated in the periphery of sinusoid in liver when the ultrastructure was examined through transmission electron microscopy. Enzymes, such as superoxide dismutase, catalase and aldehyde dehydrogenase were significantly inhibited by 22%, 38% and 15%, respectively, whereas glutathione peroxidase was constant following exposure to nanosized-TiO2.
Journal of Environmental Biology 03/2013; 34(2):283-7. · 0.68 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A proteomic analysis of the proteins in mouse brain that were differentially expressed in response to TiO2 nanoparticles was conducted to better understand the molecular mechanism of TiO2 nanoparticle-induced brain toxicity at the protein level. A total of 990 proteins from mouse brain were resolved by two-dimensional gel electrophoresis. A comparative proteomic analysis revealed that the expression levels of 11 proteins were changed by more than 2-fold in response to TiO2 nanoparticles: eight proteins were upregulated and three were downregulated by TiO2 nanoparticles. In addition, the activities of several antioxidative enzymes and acetylcholine esterase were reduced in TiO2 nanoparticle-exposed mouse brain. The protein profile alterations seem to be due to an indirect effect of TiO2 nanoparticles, because TiO2 nanoparticles were not detected in the brain in this investigation.
[Show abstract][Hide abstract] ABSTRACT: A new phycoerythrin, SCH-phycoerythrin, was purified from Synechococcus sp. ECS-18 by DEAE-Sephacel anion exchange chromatography and Sephacryl S-300 gel filtration. The protein pigment had an
absorbance maximum at 542 nm and a fluorescence maximum at 565 nm. The native molecular mass was approximately 219 kDa as
determined by gel filtration, and sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated the presence of two
subunits, with molecular mass of 19 and 17.9 kDa. These observations are consistent with the (αβ)6 subunit composition that is characteristic of phycoerythrins. The α- and β-subunits showed immunological identity by Ouchterlony
double immunodiffusion with an anti-phycoerythrin antiserum. The DNA sequence of the SCH-phycoerythrin gene was determined
by PCR amplification using primers based on the conserved N-terminal amino acid sequence of the α- and β-subunits of phycoerythrins.
Journal of Applied Phycology 01/2011; 23(1):137-142. · 2.33 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: We investigated the proteins showing differential expression in response to TiO2 nanoparticles in mouse kidney using a proteomic approach to better understand the molecular mechanism by which TiO2 nanoparticles-induced nephrotoxicity at the protein level. More than 1,100 protein spots in mouse kidney were detected by
the two-dimensional gel eletrophoresis and comparative analysis revealed that the expression of 11 proteins were changed by
more than 2 fold in response to TiO2 nanoparticles. Of these, 10 were up-regulated and 1 was down-regulated upon treatment with TiO2 nanoparticles.
Molecular and Cellular Toxicology 01/2010; 6(4):414-420. · 0.72 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: In this study, we investigated the suppressive effects of ore minerals on the allergic cell damages and oxidative cell damages. The ore minerals significantly reduced the productions of tumor necrosis factor-alpha (TNF-) and interleukin-4 (IL-4) in rat basophilic leukemia cells challenged with 2,4-dinitrophenol-bovine serum albumin (DNP-BSA). Lipoxygenase activity was also reduced by the ore minerals. Moreover, the ore minerals showed weak protective effects on the oxidative damage induced by hydrogen peroxide in pig kidney cells and retinal ganglion cells. Photohemolysis of erythrocytes in the presence of rose-bengal as a sensitizer was also inhibited by ore minerals. These results suggest that the ore minerals may be useful as the protectant for allergic and oxidative cell damages.
Journal of Environmental Science International. 01/2009; 18(12).
[Show abstract][Hide abstract] ABSTRACT: The water extracts of root, stem, and leaf from Korean indigenous Acanthopanax divaritacus were examined for their suppressive effects against allergic inflammations such as lipoxygenase activity, release, inflammatory cytokine production, and serum IgE level. The root extract inhibited the release of , a degranulation marker, from rat basophilic leukemia cells (RBL-2H3) much more potently than the stem and leaf extracts. The root extract also significantly reduced the expression of in the RBL-2H3 cells challenged with antigen. Moreover, there was a significant fall in the serum IgE level by the treatment of the root extract. Taken together, the root extract could be the most potent inhibitor of allergic inflammation, suppressing release and inflammatory cytokine expression, as well as reducing the rise of serum IgE level.
Journal of Applied Biological Chemistry 01/2007; 50(3).
[Show abstract][Hide abstract] ABSTRACT: Differentially expressed proteins in mouse liver caused by toxicity of titanium nanoparticles (TiO2 NPs) were screened. More than 1,400 protein spots in mouse liver were detected by twodimensional gel electrophoresis, and 15 proteins that showed greater than 2-fold expressional changes in response to TiO2 NPs were identified by liquid chromatography-tandem mass spectrometry. Of these, 12 proteins were down-regulated and 3 proteins were up-regulated upon treatment with TiO2 NPs. The 15 differentially expressed proteins could be used for detection of inflammation, apoptosis, and antioxidative reaction for treatment of acute hepatic damage by TiO2 NPs.
Journal of the Korean Society for Applied Biological Chemistry 54(6). · 0.43 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: In this study, the differentially expressed proteins by titanium dioxide nanoparticles (TiO2 NPs) in mouse lung were examined via proteomic approach to better understand the molecular mechanism by which TiO2 NPs could induce toxicities at the protein level. We identified eight proteins that exhibited more than two-fold changes
in expression by TiO2 NPs. Of these, five proteins, named cytoplasmic aconitase, L-lactate dehydrogenase A chain, carbonic anhydrase 1, pyruvate
kinase isoform M2 and peroxiredoxin 6 displayed increased intensities in TiO2 NP-exposed lungs, while three proteins, named heat shock protein, moesin and apolipoprotein A-1 precursor, showed reduced
KeywordsProteomics–Mouse–Lung–TiO2 nanoparticles–Protein expression
Molecular and Cellular Toxicology 7(3):283-289. · 0.72 Impact Factor