Yu-Mi Jeon

Soonchunhyang University, Onyang, Chungcheongnam-do, South Korea

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Publications (10)7.89 Total impact

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    ABSTRACT: The present study was designed to evaluate the therapeutic potential of antimicrobial photodynamic therapy (PDT) using chlorin e6 with halogen light against acne bacteria-induced inflammation. Highly purified chlorin e6 (Ce6), as a second generation photosensitizer, was synthesized from Spirulina chlorophyll. To evaluate the antimicrobial property of Ce6-mediated PDT with halogen light, the broth microdilution method and two-color fluorescence assay were used. The free radicals generated upon irradiating Ce6 with halogen light were measured using 2,7-dichlorofluorescin diacetate. Propionibacterium acnes was intradermally injected into the left ear of the ICR mice, and the anti-inflammatory effect of Ce6-mediated PDT with halogen light was measured by the histological examination. The expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) as well as pro-inflammatory cytokines were also measured by Western blotting. Chlorin e6-mediated PDT with halogen light (30,000lx) inactivated various skin bacteria, including P. acnes in a dose-dependent manner. The MIC99 value against P. acnes (KCTC3314) of Ce6 with light was >0.49μg/ml, whereas the MIC99 for Ce6 alone was >31.25μg/ml. Ce6-mediated PDT suppressed the expression of P. acnes-induced pro-inflammatory cytokines and iNOS, but not COX-2 in a mouse model. This study showed a remarkable therapeutic effect of chlorin e6-mediated PDT with halogen light against P. acnes-induced inflammation. Our results suggest for the first time the potential of Ce6-mediated PDT with halogen light as a more effective and safer alternative treatment to antibiotic therapy against pathogenic infections of the skin. Copyright © 2015. Published by Elsevier Inc.
    Life Sciences 01/2015; 124. DOI:10.1016/j.lfs.2014.12.029 · 2.30 Impact Factor
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    Yu-Mi Jeon, Mi-Young Lee
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    ABSTRACT: Abstract During screening of Korean indigenous medicinal herbs with anti-neurodegenerative activities, we found that the extract of Acanthopanax divaricatus vat. albeofructus could inhibit rotenone-induced DNA and cell damage in neuroblastoma cells. A simple in vitro model developed for the study of possible mechanisms underlying neurodegeneration in Parkinson’s disease includes the administration of rotenone to the human dopaminergic neuroblastoma cell line, SH-SY5Y. In this investigation, rotenone induced oxidative DNA damage of lymphocytes as expected. However, the oxidative DNA damage was inhibited in vitro upon treating Acanthopanax extract. Moreover, Acanthopanax extract resulted in the inhibitory activities against cell damage, ROS generation and chromatin condensation by rotenone.
    12/2013; 4(4). DOI:10.1007/s13530-012-0144-3
  • Yu-Mi Jeon, Wan-Jong Kim, Mi-Young Lee
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    ABSTRACT: The aim of the study was to examine the liver tissue damage induced by nanosized-TiO2 in mouse. The biochemical parameters of liver, namely glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase and alkaline phosphatase were enhanced approximately 18%, 35% and 69% by exposure to nanosized-TiO2, respectively. The nanosized-TiO2 accumulated in the periphery of sinusoid in liver when the ultrastructure was examined through transmission electron microscopy. Enzymes, such as superoxide dismutase, catalase and aldehyde dehydrogenase were significantly inhibited by 22%, 38% and 15%, respectively, whereas glutathione peroxidase was constant following exposure to nanosized-TiO2.
    Journal of Environmental Biology 03/2013; 34(2):283-7. · 0.55 Impact Factor
  • Yu-Mi Jeon, Seul-Ki Park, Mi-Young Lee
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    ABSTRACT: Differentially expressed proteins in mouse liver caused by toxicity of titanium nanoparticles (TiO2 NPs) were screened. More than 1,400 protein spots in mouse liver were detected by twodimensional gel electrophoresis, and 15 proteins that showed greater than 2-fold expressional changes in response to TiO2 NPs were identified by liquid chromatography-tandem mass spectrometry. Of these, 12 proteins were down-regulated and 3 proteins were up-regulated upon treatment with TiO2 NPs. The 15 differentially expressed proteins could be used for detection of inflammation, apoptosis, and antioxidative reaction for treatment of acute hepatic damage by TiO2 NPs.
    Journal of the Korean Society for Applied Biological Chemistry 12/2011; 54(6). DOI:10.1007/BF03253172 · 0.54 Impact Factor
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    ABSTRACT: In this study, the differentially expressed proteins by titanium dioxide nanoparticles (TiO2 NPs) in mouse lung were examined via proteomic approach to better understand the molecular mechanism by which TiO2 NPs could induce toxicities at the protein level. We identified eight proteins that exhibited more than two-fold changes in expression by TiO2 NPs. Of these, five proteins, named cytoplasmic aconitase, L-lactate dehydrogenase A chain, carbonic anhydrase 1, pyruvate kinase isoform M2 and peroxiredoxin 6 displayed increased intensities in TiO2 NP-exposed lungs, while three proteins, named heat shock protein, moesin and apolipoprotein A-1 precursor, showed reduced intensities. KeywordsProteomics–Mouse–Lung–TiO2 nanoparticles–Protein expression
    Molecular and Cellular Toxicology 09/2011; 7(3):283-289. DOI:10.1007/s13273-011-0034-9 · 0.83 Impact Factor
  • Yu-Mi Jeon, Seul-Ki Park, Mi-Young Lee
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    ABSTRACT: A proteomic analysis of the proteins in mouse brain that were differentially expressed in response to TiO2 nanoparticles was conducted to better understand the molecular mechanism of TiO2 nanoparticle-induced brain toxicity at the protein level. A total of 990 proteins from mouse brain were resolved by two-dimensional gel electrophoresis. A comparative proteomic analysis revealed that the expression levels of 11 proteins were changed by more than 2-fold in response to TiO2 nanoparticles: eight proteins were upregulated and three were downregulated by TiO2 nanoparticles. In addition, the activities of several antioxidative enzymes and acetylcholine esterase were reduced in TiO2 nanoparticle-exposed mouse brain. The protein profile alterations seem to be due to an indirect effect of TiO2 nanoparticles, because TiO2 nanoparticles were not detected in the brain in this investigation.
    Animal cells and systems the official publication of the Zoological Society of Korea 06/2011; 15(2):107-114. DOI:10.1080/19768354.2011.555144 · 0.35 Impact Factor
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    ABSTRACT: A new phycoerythrin, SCH-phycoerythrin, was purified from Synechococcus sp. ECS-18 by DEAE-Sephacel anion exchange chromatography and Sephacryl S-300 gel filtration. The protein pigment had an absorbance maximum at 542 nm and a fluorescence maximum at 565 nm. The native molecular mass was approximately 219 kDa as determined by gel filtration, and sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated the presence of two subunits, with molecular mass of 19 and 17.9 kDa. These observations are consistent with the (αβ)6 subunit composition that is characteristic of phycoerythrins. The α- and β-subunits showed immunological identity by Ouchterlony double immunodiffusion with an anti-phycoerythrin antiserum. The DNA sequence of the SCH-phycoerythrin gene was determined by PCR amplification using primers based on the conserved N-terminal amino acid sequence of the α- and β-subunits of phycoerythrins.
    Journal of Applied Phycology 01/2011; 23(1):137-142. DOI:10.1007/s10811-010-9554-2 · 2.49 Impact Factor
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    ABSTRACT: We investigated the proteins showing differential expression in response to TiO2 nanoparticles in mouse kidney using a proteomic approach to better understand the molecular mechanism by which TiO2 nanoparticles-induced nephrotoxicity at the protein level. More than 1,100 protein spots in mouse kidney were detected by the two-dimensional gel eletrophoresis and comparative analysis revealed that the expression of 11 proteins were changed by more than 2 fold in response to TiO2 nanoparticles. Of these, 10 were up-regulated and 1 was down-regulated upon treatment with TiO2 nanoparticles.
    Molecular and Cellular Toxicology 12/2010; 6(4):414-420. DOI:10.1007/s13273-010-0055-9 · 0.83 Impact Factor
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    Yu-Mi Jeon, Jum-Ji Kim, Mi-Young Lee
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    ABSTRACT: In this study, we investigated the suppressive effects of ore minerals on the allergic cell damages and oxidative cell damages. The ore minerals significantly reduced the productions of tumor necrosis factor-alpha (TNF-) and interleukin-4 (IL-4) in rat basophilic leukemia cells challenged with 2,4-dinitrophenol-bovine serum albumin (DNP-BSA). Lipoxygenase activity was also reduced by the ore minerals. Moreover, the ore minerals showed weak protective effects on the oxidative damage induced by hydrogen peroxide in pig kidney cells and retinal ganglion cells. Photohemolysis of erythrocytes in the presence of rose-bengal as a sensitizer was also inhibited by ore minerals. These results suggest that the ore minerals may be useful as the protectant for allergic and oxidative cell damages.
    01/2009; 18(12). DOI:10.5322/JES.2009.18.12.1391
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    ABSTRACT: The water extracts of root, stem, and leaf from Korean indigenous Acanthopanax divaritacus were examined for their suppressive effects against allergic inflammations such as lipoxygenase activity, release, inflammatory cytokine production, and serum IgE level. The root extract inhibited the release of , a degranulation marker, from rat basophilic leukemia cells (RBL-2H3) much more potently than the stem and leaf extracts. The root extract also significantly reduced the expression of in the RBL-2H3 cells challenged with antigen. Moreover, there was a significant fall in the serum IgE level by the treatment of the root extract. Taken together, the root extract could be the most potent inhibitor of allergic inflammation, suppressing release and inflammatory cytokine expression, as well as reducing the rise of serum IgE level.
    Journal of Applied Biological Chemistry 01/2007; 50(3).