Youling Zeng

Xinjiang University, Hsin-chien, Jiangxi Sheng, China

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Publications (4)10.13 Total impact

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    ABSTRACT: According to sequences of H(+)-pyrophosphatase genes from GenBank, a new H(+)-pyrophosphatase gene (KfVP1) from the halophyte Kalidium foliatum, a very salt-tolerant shrub that is highly succulent, was obtained by using reverse transcription PCR and rapid amplification of cDNA ends methods. The obtained KfVP1 cDNA contained a 2295 bp ORF and a 242 bp 3'-untranslated region. It encoded 764 amino acids with a calculated molecular mass of 79.78 kDa. The deduced amino acid sequence showed high identity to those of H(+)-PPase of some Chenopodiaceae plant species. Semi-quantitative PCR results revealed that transcription of KfVP1 in K. foliatum was induced by NaCl, ABA and PEG stress. Transgenic lines of A. thaliana with 35S::KfVP1 were generated. Three transgenic lines grew more vigorous than the wild type (ecotype Col-0) under salt and drought stress. Moreover, the transgenic plants accumulated more Na(+) in the leaves compared to wild type plants. These results demonstrated that KfVP1 from K. foliatum may be a functional tonoplast H(+)-pyrophosphatase in contributing to salt and drought tolerance.
    Molecular Biology Reports 04/2012; 39(8):7989-96. · 2.51 Impact Factor
  • Lin Liu, Youling Zeng, Xinyan Pan, Fuchun Zhang
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    ABSTRACT: The full-length cDNAs of two Karelinia caspica genes, KcNHX1 and KcNHX2, were isolated by RACE and RT-PCR based on the conserved regions of Na(+)/H(+) antiporter (NHX) genes from other halophyte species. The cloned KcNHX1 cDNA contained 2,022 nucleotides with an open reading frame (ORF) of 1,620 bp and the KcNHX2 cDNA contained 1,976 nucleotides with an ORF of 1,653 bp. The deduced amino acid sequences indicated that both genes were homologous to NHXs from other higher plants. To investigate the possible roles of KcNHX1 and KcNHX2 in the salt stress response of K. caspica and the underlying regulatory mechanisms, RNAi vectors were constructed and transformed into K. caspica to specifically silence endogenous KcNHX1 and KcNHX2. The physiological results showed that silencing KcNHX1 in K. caspica led to reduced salt tolerance in high concentrations of NaCl, suggesting that KcNHX1 plays an essential role in the response of K. caspica to salt stress. However, the inhibition of KcNHX2 seemed to have little influence on the salt resistance of transgenic plants, indicating that KcNHX2 may be relevant for functions other than salt tolerance in K. caspica.
    Molecular Biology Reports 02/2012; 39(6):7193-202. · 2.51 Impact Factor
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    ABSTRACT: According to sequences of several vacuolar Na(+)/H(+) antiporter genes from Xinjiang halophytic plants, a new vacuolar Na(+)/H(+) antiporter gene (HcNHX1) from the halophyte Halostachys caspica was obtained by RACE and RT-PCR using primers corresponding to conserved regions of the coding sequences. The obtained HcNHX1 cDNA was 1,983 bp and contained a 1,656 bp open reading frame encoding a deduced protein of 551 amino acid residues. The deduced amino acid sequence showed high identity with other NHX1 we have cloned previously from halophyte in Xinjiang desert area. The phylogenetic analysis showed that HcNHX1 formed a clade with NHX homologs of Chenopodiaceae. Expression profiles under salt treatment and ABA induction were investigated, and the results revealed that expression of HcNHX1 was induced by NaCl and ABA. To compare the degree of salt tolerance, we over-expressed HcNHX1 in Arabidopsis. Two transgenic lines grew more vigorously than the wild type (WT) under salt stress. The analysis of ion contents indicated that under salt stress, the transgenic plants compartmentalized more Na(+) in the leaves compared with wild-type plants. Together, these results suggest that the products of the novel gene HcNHX1 from halophyte Halostachys caspica is a functional tonoplast Na(+)/H(+) antiporter.
    Molecular Biology Reports 10/2010; 38(3):1889-99. · 2.51 Impact Factor
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    ABSTRACT: To understand the molecular and cellular mechanisms underlying salinity stress tolerance in the halophyte Halostachys caspica, a suppression subtractive hybridization forward library was constructed using plants treated with 600 mM NaCl for 0, 6, 12, 24, 36, and 48 h. A total of 86 unique ESTs were identified as positive clones by reverse Northern dot-blotting and submitted to the Genbank database. Of these ESTs, 53 have sequence similarity with known proteins, 31 have sequence similarity with unidentified sequences, and 2, which could not be annotated, may represent novel genes. The annotated sequences were classified into nine different categories according to their putative functions, and many were found to be homologous to genes involved in the response to abiotic stress. Six ESTs were randomly selected to assess their transcript levels under salt stress; although they exhibited different expression patterns at different time points, all six tended to be induced by salt treatment. The ESTs identified in the present study as genes induced by salt stress may help elucidate the factors in H. caspica that underlie the extreme salt tolerance of this species.
    Plant Cell Tissue and Organ Culture 110(1). · 2.61 Impact Factor