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Jimin Sun,
Shuying Luo,
Junfen Lin,
Jinhua Chen,
Juan Hou,
Tao Fu,
Huakun Lv,
Zhiping Chen,
Liming Cong,
Feng Ling,
Chengliang Chai, Yanjun Zhang,
Haiyan Mao,
Juying Yan,
Yiyu Lu,
Qiyong Liu,
Xiuping Song,
Liang Lu
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ABSTRACT: Abstract Dengue fever (DF) is often asymptomatic in endemic areas. Asymptomatic infection during a DF outbreak in China, where DF is not endemic, has not been reported until now. In this study a total of 365 subjects from 6 villages were recruited from October 4-7, 2009. Overall, 102 subjects (27.95%) were positive for dengue virus (DENV) IgM, and 14 subjects (3.84%) were positive for DENV IgG and IgM. In different age groups, seropositive rates varied from 12.50% to 50.00% for DENV IgM, and from 0% to 11.76% for DENV IgG. Seroprevalence of DENV IgM was significantly higher than that of DENV IgG. Seroprevalence rates of DENV IgM differed among different villages. However, the seroprevalence of DENV IgM was not statistically significantly different among gender and age groups. Asymptomatic DF infection is prevalent in non-endemic areas.
Viral immunology 12/2012; 25(6):456-460. · 1.78 Impact Factor
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ABSTRACT: BACKGROUND: Hand, foot and mouth disease (HFMD) is caused by members of the family Picornaviridae in the genus Enterovirus. It has been reported that coxsackievirus A6 (CVA6) infections are emerging as a new and major cause of epidemic HFMD. Sporadic HFMD cases positive for CVA6 were detected in the mainland of China in recent years. To strengthen the surveillance of CVA6 infections and outbreak control, the clinical diagnosis is urgently needed to distinguish the CVA6 infection disease from other infections. METHODS: In order to develop a sensitive quantitative real-time RT-PCR assay for rapid detection of CVA6 RNA, primers and probe were designed to target the VP1 gene segment of CVA6. The conservation of the target segment was firstly analyzed by bioinformatic technology. The specificity of the real-time RT-PCR was further confirmed by detecting other related viruses and standard curves were established for the sensitivity evaluation. The pharyngeal swab samples from the EV71 and CVA16 unrelated HFMD patients were applied for CVA6 detection through the established method. RESULTS: Based on the primer--probe set to detect the target VP1 gene segment of CVA6, the quantitative real-time RT-PCR assay could discriminate CVA6 infection from other resemble viral diseases with a potential detection limit of 10 viral copies/ml. The specificity of the assay was determined by sequence alignment and experimentally tested on various related viruses. The standard curve showed that the amplification efficiency of templates with different concentrations of templates was almost the same (R2 >0.99). Evaluation of the established method with pharyngeal swabs samples showed good accordance with the results from serology diagnosis. CONCLUSION: This study is the first report developing a VP1 gene-based quantitative real-time RT-PCR for rapid, stable and specific detection of CVA6 virus. The real-time RT-PCR established in this study can be used as a reliable method for early diagnosis of CVA6 infection.
Virology Journal 11/2012; 9(1):298. · 2.34 Impact Factor
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International journal of infectious diseases: IJID: official publication of the International Society for Infectious Diseases 10/2012; · 2.17 Impact Factor
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ABSTRACT: Biofilm formation is a well-known pathogenic mechanism in infections caused by Acinetobacter baumannii. Recently, a biofilm synthesis-associated gene has been found in A. baumannii ATCC19606. Bioinformatic analysis showed 2 transmembrane structures and an hmsS superfamily domain, which was related to biofilm formation. What is more, high homology sequences of the bfs gene were only present in A. baumannii spp., and the similarities of nucleotide sequences of the bfs gene from A. baumannii strains ATCC17978, ACICU, S1, AB307-0294, and AB0057 compared with the reported sequence of bfs (GenBank accession No.: NZ_GG704572) were all above 95%. The distribution and conservation of the bfs gene from clinically derived A. baumannii strains were verified through conventional polymerase chain reaction (PCR). After this, we established a bfs gene-based real-time quantitative PCR assay to detect A. baumannii. Species specificity and sensitivity assays were designed and validated. By using this method, all the A. baumannii strains separated from clinical samples were identified and showed good accordance with the results from biochemical identification. This study is the first report of developing a bfs gene-based quantitative polymerase chain reaction for rapid, stable, and specific detection of A. baumannii. This method can be applied to clinical laboratory diagnosis, and detection of A. baumannii present on medical instruments.
Diagnostic molecular pathology: the American journal of surgical pathology, part B 12/2011; 20(4):242-8. · 1.58 Impact Factor
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Jimin Sun,
Junfen Lin,
Juying Yan,
Weizhong Fan,
Liang Lu,
Huakun Lv,
Juan Hou,
Feng Ling,
Tao Fu,
Zhiping Chen,
Liming Cong,
Qiyong Liu, Yanjun Zhang,
Chengliang Chai
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ABSTRACT: To the Editor: Beginning in July 2009, physicians in the city of Yiwu, Zhejiang Province, People's Republic of China, noted an outbreak of illness characterized by rash, headache, subjective fever, itching, anorexia, and arthritis. We present the results of the investigation of this outbreak, which was caused by dengue virus (DENV) serotype 3 (DENV-3) subtype III.
Emerging Infectious Diseases 02/2011; 17(2):321-3. · 6.79 Impact Factor
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Hui Dong, Yanjun Zhang,
Hui Xiong,
An Yan,
Guohui Ding,
Yangyi Chen,
Liqun Xie,
Jiazheng Chen,
Guoqing Zhang,
Pei Hao,
Liming Cong,
Yiyu Lu,
Xiaoyan Che,
Xiaoning Wang,
Yixue Li,
Kwok-Yung Yuen,
Guoping Zhao,
Weirong Jin
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ABSTRACT: The novel influenza A (H1N1) virus is now rapidly spreading across the world. Early detection is one of the most effective measures to prevent further transmission of the virus. 4 sets of proprietary primers and probes designed for detection of influenza A viruses (InfA), human and swine H1N1 viruses (SH1), the novel H1N1 viruses (NH1) and RNaseP gene (RP) respectively were pooled together in a single tube for a multi-fluorescent real-time RT-PCR assay. The detection limit was found to be one order more sensitive than that employing the WHO recommended protocol. The NH1 probe was negative for all control samples including human seasonal H1N1 virus, other subtypes of human influenza A viruses (H3, H5, H9), human influenza B virus and nasopharyngeal swabs from patients with noninfluenza respiratory diseases, indicating its high specificity, capable of discriminating the novel influenza A virus from the previously identified H1N1 viruses. For confirmation, the PCR amplified fragment of the hemagglutinin gene was sequenced which could provide enough information to identify the novel H1N1 virus as a distinct cluster among all viruses of subtype H1 through average distance clustering analysis. Although these assays should be useful in the current outbreak for rapid detection and discrimination of the novel H1N1 from swine H1N1 and other human seasonal H1N1 viruses, further design improvement is suggested to match possible sequence variations in the detected region along with the course of the epidemic.
Virus Research 10/2009; 147(1):85-90. · 2.94 Impact Factor
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Lanjuan Li,
Zhigang Wang,
Yiyu Lu,
Qiyu Bao,
Suhong Chen,
Nanping Wu,
Suyun Cheng,
Jingqing Weng, Yanjun Zhang,
Juying Yan, [......],
Yingpu Yu,
Minli Zhang,
Minhong Li,
Jun Yao,
Qunying Lu,
Pingping Yao,
Xiaochen Bo,
Jianer Wo,
Shengqi Wang,
Songnian Hu
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ABSTRACT: To study the severe acute respiratory syndrome (SARS)-associated coronavirus genotype and its characteristics.
A SARS-associated coronavirus isolate named ZJ01 was obtained from throat swab samples taken from a patient in Hangzhou, Zhejing province. The complete genome sequence of ZJ01 consisted of 29,715 bp (GenBank accession: AY297028, version: gi: 30910859). Seventeen SARS-associated coronavirus genome sequences in GenBank were compared to analyze the common sequence variations and the probability of co-occurrence of multiple polymorphisms or mutations. Phylogenetic analysis of those sequences was done.
By bioinformatics processing and analysis, the 5 loci nucleotides at ZJ01 genome were found being T, T, G, T and T, respectively. Compared with other SARS-associated coronavirus genomes in the GenBank database, an A/G mutation was detected besides the other 4 mutation loci (C:G:C:C/T:T:T:T) involved in this genetic signature. Therefore a new definition was put forward according to the 5 mutation loci. SARS-associated coronavirus strains would be grouped into two genotypes (C:G:A:C:C/T:T:G:T:T), and abbreviated as SARS coronavirus C genotype and T genotype. On the basis of this new definition, the ZJ01 isolate belongs to SARS-associated coronavirus T genotype, first discovered and reported in mainland China. Phylogenetic analysis of the spike protein gene fragments of these SARS-associated coronavirus strains showed that the GZ01 isolate was phylogenetically distinct from other isolates, and compared with groups F1 and F2 of the T genotype, the isolates of BJ01 and CUHK-W1 were more closely related to the GZ01 isolate. It was interesting to find that two (A/G and C/T) of the five mutation loci occurred in the spike protein gene, which caused changes of Asp to Gly and Thr to Ile in the protein, respectively.
Attention should be paid to whether these genotype and mutation patterns are related to the virus's biological activities,epidemic characteristics and host clinical symptoms.
Chinese medical journal 10/2003; 116(9):1288-92. · 0.86 Impact Factor
