Ying Xiao

Publications (2)0 Total impact

• Article: [Histopathologic and ultrastructure changes of corneal dystrophy of bowman layer, type I].

  Qi Wang, Jiaqi Chen, Ying Xiao
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  ABSTRACT: To explore the histopathologic and ultrastructure changes of corneal dystrophies of bowman layer, type I (CDB I ) in Chinese. Cornea buttons were obtained from 4 CDB I patients in 2 pedigrees who underwent lamella or penetrating keratoplasty. Sections with HE and special staining were observed under light microscope. Ultrathin sections were performed and ultrastructure changes were investigated under transmission electron microscope. Two normal cornea specimens were used as control. In the CDB I cornea epithelium, microvillus were decreased or lost. Small clumps of deposits with high electron density were found between basal cells. Some basal cells appeared apoptosis sign. A great quantity of rod-shape deposits were seen in Bowman's layer and the anterior part of stroma. CDB I patients have characteristic corneal structure changes, and may be one of the reasons of the clinical symptoms.
  Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu 07/2005; 21(2):103-7.
• Article: [Construction of HESR1 siRNA plasmid expression vector].

  Bin Li, Shibo Tang, Shaofen Lin, Jing Meng, Ying Xiao
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  ABSTRACT: To construct of HESR1 siRNA plasmid expression vector. Cultures human umbilicus vein endothelial cell, according to the computer aided design, HESR1-specific siRNA gene was synthesized and cloned into the RNAi-Ready pSIREN-RetroQ ZsGreen Vector. The constructed HESR1-RNAi plasmid were transfected into human umbilicus vein endothelial cell, RT-PCR detected the effected of HESR1-RNAi plasmid. The recombinant was cloned and the sequence was obtained, HESR1-RNAi plasmid can down-regulated the expression of HESR1 in human umbilicus vein endothelial cell. Conclusion : Successfully cloning the recombinant make it possible searching new mechanism of HESRI in angiogenesis.
  Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu 07/2005; 21(2):112-5.