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Publications (4)1.32 Total impact

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    ABSTRACT: Harlequin ichthyosis (HI) is a rare congenital fetal skin keratinization disorder with an autosomal recessive inheritance. HI has specific sonographic features in the antenatal period. Several reports of prenatal sonographic diagnosis of HI have demonstrated its characteristic facial features that include a persistently open mouth and ectropion, with echogenic amniotic fluid and restriction of limb movements. Here, we report the case of a fetus in the 3rd trimester with these syndromic features identified by two- and three-dimensional sonography. Our ultrasonographic observation of membranes arising from the skin surface is particularly noteworthy. No prior case in which HI has been characterized by three-dimensional ultrasonography has been reported.
    Journal of Medical Ultrasound 01/2013;
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    ABSTRACT: To make sure whether or not Bcrp1 is the marker of cervical cancer stem cells or not by studying the invasive ability and formation of tumors of Bcrp1(+) phenotype HeLa cells. The tumor cell migration and invasion assay were used by boyden chamber to identify the invasive ability of Bcrp1(+) phenotype HeLa cells. The formation of tumors in vivo experiments were completed, in which the two groups of cells with different concentrations were inoculated in non obese diabetes-severe combined immunodeficiency disease (NOD/SCID) mice (1×10(4), 1×10(5), 1×10(6)/ml), and the differences of time, rate and volume in the formation of tumors between two groups were observed. (1) In the invasion assay, the amount of cells that invaded through the artificial basement membrane in Bcrp1(+) group were 99 ± 14, which was significantly greater than those in Bcrp1(-) group (57 ± 13, P < 0.05);the length of the Bcrp1(+) group was (366 ± 52) µm, which was significantly greater than the Bcrp1(-) group (301 ± 54) µm (P < 0.05). (2) Following transplantation of 1×10(4) cells, only the Bcrp1(+) cells formed tumors in NOD/SCID mice. When 1×10(5) or 1×10(6) cells were transplanted, the tumor incidence and the tumor mass were greater in the Bcrp1(+) groups than those in the Bcrp1(-) groups (P < 0.05). Bcrp1(+) HeLa cell have the greater capacity of invasive and the tumorigenicity, which may contain cancer stem cells.
    Zhonghua fu chan ke za zhi 07/2012; 47(7):526-9.
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    ABSTRACT: Cervical cancer is one of the most common gynecologic malignancies and poses a serious health problem worldwide. Identification and characterization of cervical cancer stem cells may facilitate the development of novel strategies for the treatment of advanced and metastatic cervical cancer. Breast cancer-resistance protein (Bcrp1)-positive cells were selected from a population of parent HeLa cells using flow cytometry. The invasion capacity of Bcrp1-positive and -negative cells was analyzed with a Boyden chamber invasion test. The tumorigenicity of these cells was determined by in vivo transplantation in non-obesity diabetes/severe combined immunodeficiency (NOD/SCID) mice. The Bcrp1-positive subpopulation accounted for about 7% of the parent HeLa cell population. The proliferative capacity of the Bcrp1-positive cells was greater than that of the Bcrp1-negative cells (P < 0.05). In the invasion assay, the Bcrp1-positive cells demonstrated a greater invasive capacity through the artificial basement membrane than their Bcrp1-negative counterparts. Following transplantation of 10(4) cells, only the Bcrp1-positive cells formed tumors in NOD/SCID mice. When 10(5) or 10(6) cells were transplanted, the tumor incidence and the tumor mass were greater in the Bcrp1-positive groups than those in the Bcrp1-negative groups (P < 0.05). The Bcrp1-positive subpopulation cervical cancer stem cells.
    Cytotechnology 03/2012; 64(4):477-84. · 1.32 Impact Factor
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    ABSTRACT: To make sure whether Bcrp1 is the marker of cervical cancer stem-like cells or not by studying the characterization of Bcrp1(+) HeLa cells. Immunofluorescence stained flow cytometry and electron microscope were used to sort and observe ultrastructures of Bcrp1(+) and Bcrp1(-) HeLa cells. Flow cytometry was used to identify the cycle and the rate of apoptosis with annexin V in two group cells. The expression of proliferating cell nuclear antigen (PCNA) and caspase-3 were tested using western blot method. (1) There were 7.1% Bcrp1(+) cells and 92.9% Bcrp1(-)cells in HeLa cells. Bcrp1(+) HeLa cells were large in size of nuclear and nucleoli are clear, and there were rich of cytomicrosome and rough endoplasmic reticulum. After sorted and cultured for 24, 48, 72 hours, the adhesion in Bcrp1(+) cells were 72.8%, 81.1%, 80.4%, respectively. While, they were 3.3%, 18.7%, 12.6% at each time for Bcrp1(-) cells (all P < 0.05). (2) There are more S phase cells in Bcrp1(+) cells than that in Bcrp1(-) cells (54.1% vs 21.1%, P < 0.05), while the percentage of G(0)/G(1) and G(2)/M in Bcrp1(-) cells were higher than those in Bcrp1(+) cells (53.0% vs 44.4%, 25.9% vs 1.5%; all P < 0.05). The rate of apoptosis in Bcrp1(+) cells was lower than that in Bcrp1(-) cells (0.2% vs 5.3%, P < 0.05). (3) The expression of PCNA in Bcrp1(+) cells was higher than that in Bcrp1(-) cells (3140 vs 2255, P < 0.05), while the expression of caspase-3 of Bcrp1(+) cells was lower than that in Bcrp1(-) cells (1970 vs 3551, P < 0.05). There are more vigor and ability of proliferation and lower rate of apoptosis in Bcrp1(+) HeLa cells than those in Bcrp1(-) cells, which may be some characters of cervical cancer stem cells.
    Zhonghua fu chan ke za zhi 07/2010; 45(7):525-9.