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Ting Hu,
Tao Peng,
Xiao-Juan Li,
Dong-Dong Chen,
Han-Hui Dai, Xiao-Jun Deng,
Zhen-Feng Yue,
Guo-Min Wang,
Jian-Zhong Shen,
Xi Xia,
Shuang-Yang Ding,
Yue-Ning Zhou,
Ai-Ling Zhu,
Hai-Yang Jiang
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ABSTRACT: An ultra-high-performance liquid chromatography with tandem mass spectrometric detection (UHPLC-MS/MS) method was established for the simultaneous determination of residues of thirty non-steroidal anti-inflammatory drugs (NSAIDs) in swine muscle. The samples were extracted with acetonitrile and phosphoric acid. The extracts were defatted with n-hexane, and then purified by HLB solid-phase extraction cartridge. Analysis was carried out on UHPLC-ESI-MS/MS working with multiple reaction monitoring mode with polarity switching. Limits of detection were between 0.4 μg/kg and 2.0 μg/kg, and limits of quantification were between 1.0 μg/kg and 5.0 μg/kg. The recoveries of NSAIDs were between 61.7% and 125.7% at spiked levels of 1.0-500 μg/kg. The repeatability was less than 8% and the within-laboratory reproducibility was not more than 12.3%. The method was reliable, convenient and sensitive.
Journal of chromatography. A 11/2011; 1219:104-13. · 4.19 Impact Factor
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ABSTRACT: To observe that antiretroviral efficacy, immune reconstitution of four-year highly active antiretroviral therapy (HAART), and evaluate its side effect in Chinese HIV-1-infected patients.
A total of 258 HIV-1 infected patients, given HAART regimens composed of two nucleoside reverse transcriptase inhibitor (NRTI) and one non-nucleoside reverse transcriptase inhibitor (NNRTI) for mean 51.5 months, measured HIV RNA viral load (VL) and the counts of CD(4)(+) T cell, CD(8)(+) T cell at the baseline and 6, 12, 24, 36 and 48 months after HAART initiation, respectively, monitoring side effect, blood routine, main biochemical parameters, and other disadvantageous accidents during the 51.5-month treatment.
Plasma HIV-1 RNA level was determined by fluorescent quantitative polymerase chain reactions (FQ-PCR) at the baseline and 6, 12, 24, 36 and 48 months after starting HAART, and showed 5.27, 2.97, 2.74, 2.62, 2.67 and 2.75 lg (copies/ml), respectively. The counts of CD(4)(+) T cell from (127 ± 63) cells/µl at the baseline increased to (190 ± 115), (248 ± 93), (269 ± 127), (296 ± 156) and (317 ± 195) cells/µl at 6, 12, 24, 36 and 48 months after starting HAART. A total of 149 treated patients (57.8%)had gastrointestinal side effects, peripheral polyneuropathy, various rashes, central nervous system disorders, fever or baldness. Twenty-two patients changed one of three medicines to another because toxicity. Sixteen changed the regimen to the second line HAART for lactic acidosis or other serious toxicities.
A total of 258 HIV-1 infected Chinese patients treated with two NRTI and one NNRTI as first line HAART regimen during mean 51.5 months, showed a good antiretroviral efficacy and immune reconstitution, but a few side-effects at the parts of patients. It is necessary to treat adverse effect and change HAART regimen for severe toxicity in time.
Zhonghua nei ke za zhi [Chinese journal of internal medicine] 03/2011; 50(3):230-4.
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ABSTRACT: Utilizing a solid phase extraction column (MCT) containing mixed hydrophilic functional gel and cation exchange sorbent, a sensitive and rapid HPLC-MS/MS method for simultaneously determining the residues of melamine (MEL) and cyanuric acid (CYA) in human foodstuffs was developed. MEL and CYA in egg, pork, liver, kidney and pork, shrimp, sausage casing, honey, soybean milk, soybean powder and dairy product were extracted using acetonitrile/water, defatted with hexane and isolated using MCT solid phase extraction column. The residues were separated upon a hydrophilic interaction liquid chromatography (HILIC) column and analyzed by electrospray ionization under negative-positive switched mode on a triplequadrupole mass spectrometry. The selected reaction monitoring was performed on [M+H](+) of m/z 127.9 to provide the transition of 127>85 and 127>68 (MEL) while the [M-H](-) of m/z 127.1 was selected as the precursor ion for CYA resulting in product ions m/z 85 and 42. Isotope labeled internal standard ((15)N(3)-MEL and (13)C(3)-CYA) and matrix-matched calibration were both used to observe the recovery to be 70.0-129.6% and 70.0-128.9% with RSD of 1.4-23.3% and 1.5-21.7% for MEL and CYA, respectively (n=6). All the LODs and LOQs of MEL and CYA were less than 39.4 and 99.1μgkg(-1), respectively, in 18 matrices, which were sensitive enough for quantitative analysis. This method has been proven effective in simultaneous determination of melamine and cyanuric acid when inspecting unknown and positive samples.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 10/2010; 878(28):2839-44. · 2.78 Impact Factor
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ABSTRACT: Plant volatiles mainly include terpenoid, benzenoid/phenylpropanoid and fatty-acid derivatives, and they play diverse roles in plant-plant and plant-insect communications. In recent years, great progress has been made in biochemical and molecular characterization of the formation and release of these volatiles, and this make it possible to dissect their biological functions. More than 30 kinds of genes related with biosynthesis of these volatiles have been cloned. The pathways leading to synthesis of these metabolites can be controlled by enzyme activity and the substrates available as well as gene architecture. Plant volatiles can serve as attractants for specific pollinators, important cellular regulators in developmental processes and protection against environmental stress, whereas those released after herbivory or pathogen attacks can induce the expression a set of defensive genes or attraction of predators of the herbivores. An attractive prospect in this area is to design scent spectrum of plant with genetic and molecular technology. Two alternative approaches are used to genetically engineer these plant volatiles. One is based on the introduction of foreign genes encoding enzymes with activities that are missing in target plant, while the other is focused on modulating the expression of native genes.
Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology 03/2004; 30(1):11-8.
