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ABSTRACT: A prospective randomized controlled trial was performed in elderly patients with benign prostatic hyperplasia (BPH) to evaluate the clinical effectiveness of channel transurethral resection of the prostate (C-TURP) combined with an interstitial laser coagulation (ILC) technique during a 4-year follow-up period.
A total of 150 consecutive BPH patients were randomized to an ILC+C-TURP group (n = 50), an ILC group (n = 50) and a TURP group (n = 50). Urinary tract infection, acute urinary retention and retrograde ejaculation were monitored, and the retreatment rate, international prostate symptom score (IPSS) and maximum flow rate (Q(max)) were measured.
A total of 142 patients completed the follow-up and were recruited for further analysis. At 1 month, the proportion of patients with urinary tract infection was similar between the C-TURP+ILC group and the TURP group (8.5 and 6.5%, p > 0.05), but significantly higher than that in the ILC group (51%, p < 0.001). Acute urinary retention was found in 30.6% of patients in the ILC group, but was not observed in the C-TURP+ILC and TURP groups. In the TURP group, the rate of retrograde ejaculation was significantly higher than that in the other 2 groups (p < 0.001). The retreatment rate was 8.5, 36.7 and 2.2% in the C-TURP+ILC, ILC and TURP groups, respectively (p < 0.001). When compared with baseline, the IPSS in the C-TURP+ILC, ILC and TURP groups was decreased by 70.6, 45.4, and 81.0%, respectively (ILC vs. C-TURP+ILC or TURP, p < 0.01) at the 48-month follow-up. One month after surgery, the Q(max) was significantly increased in the C-TURP+ILC group and the TURP group when compared with that at baseline (p < 0.01). The TURP group had the highest and the ILC group had the lowest increase in the Q(max) at the 12-, 24-, and 48-month follow-ups (p < 0.05).
C-TURP+ILC is a safe and effective modality for the treatment of BPH, and exhibits favorable short-term clinical response and long-term durability. It is relatively reasonable and acceptable for treatment of high-risk elderly patients or those with a limited life expectancy.
Urologia Internationalis 09/2011; 87(4):392-9. · 0.99 Impact Factor
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ABSTRACT: Our previous study has demonstrated that the rapid tolerance to cerebral ischemia by electroacupuncture (EA) pretreatment was possibly mediated through an endocannabinoid system-related mechanism. The purpose of this study was to investigate whether activation of epsilon protein kinase C (εPKC) was involved in EA pretreatment-induced neuroprotection via cannabinoid receptor type 1 in a rat model of transient focal cerebral ischemia.
The activation of εPKC in the ipsilateral brain tissues after EA pretreatment was investigated in the presence or absence of cannabinoid receptor antagonists. At 2 hours after the end of EA pretreatment, focal cerebral ischemia was induced by middle cerebral artery occlusion for 120 minutes in rats. The neurobehavioral scores, infarction volumes, neuronal apoptosis, and the expression of Bcl-2 and Bax were evaluated after reperfusion in the presence or absence of εPKC-selective peptide inhibitor (TAT-εV1-2) or activator (TAT-ψεRACK).
EA pretreatment enhanced εPKC activation. Systemic delivery of TAT-ψεRACK conferred neuroprotection against a subsequent cerebral ischemic event when delivered 2 hours before ischemia. Pretreatment with EA reduced infarct volumes, improved neurological outcome, inhibited neuronal apoptosis, and increased the Bcl-2-to-Bax ratio after reperfusion, and the beneficial effects were attenuated by TAT-εV1-2. In addition, the blockade of cannabinoid receptor type 1, but not cannabinoid receptor type 2 receptor, reversed the increase in εPKC activation and neuroprotection induced by EA pretreatment.
EA pretreatment may activate endogenous εPKC-mediated anti-apoptosis to protect against ischemic damage after focal cerebral ischemia via cannabinoid receptor type 1, which represents a new mechanism of EA pretreatment-induced rapid tolerance to focal cerebral ischemia in rats.
Stroke 02/2011; 42(2):389-96. · 5.73 Impact Factor
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Expert Syst. Appl. 01/2010; 37:8850-8860.
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Advanced Data Mining and Applications, 5th International Conference, ADMA 2009, Beijing, China, August 17-19, 2009. Proceedings; 01/2009
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ABSTRACT: To evaluate the extrinsic stain removal benefit of a sodium hexametaphosphate containing dual-phase dentifrice (Crest Vivid White) relative to a marketed negative control (Colgate Cavity Protection).
This was a parallel groups, examiner-blind, randomized and controlled clinical trial. A total of 200 healthy adults with natural stain on their anterior teeth were enrolled into the study. Following baseline examination, subjects were randomly assigned to one of the two treatment groups based on baseline Lobene composite scores, smoking status (yes/no), tea/coffee consumption (yes/no), and gender. Subjects brushed twice daily, at least 1 minute every time over 6 weeks. Clinical examinations including extrinsic stain evaluation and oral soft tissue examination were conducted at BL, Weeks 3 and 6. Extrinsic stain removal was evaluated on the anterior teeth by a dental examiner using Lobene stain index.
Of the 200 subjects who were randomized to treatment, 195 were available for the 3-week examination and 193 subjects completed the study. The dual-phase dentifrice exhibited a statistically significant (P< 0.01) reduction in Lobene stain composite scores when compared to the negative control dentifrice at Week 6. Adjusted mean Lobene composite reduction for the dual-phase dentifrice group (0.32) was twice as big as the negative control group (0.16). Change in Lobene stain extent (area) contributed primarily to the overall composite score reduction. All test products were well tolerated over the 6-week treatment period.
American journal of dentistry 09/2007; 20(4):227-30. · 0.76 Impact Factor
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ABSTRACT: We cloned a novel mouse cDNA, Mcpr1 (mouse cleft palate-related gene 1), between retinoic acid (RA)-treated murine embryonic palatal and control shelves by improved subtractive hybridization. Its transcript was identified by Northern blotting. The open reading frame encodes 132 amino acids and shows almost no identity to other genetic products. Mcpr1 expression could be detected extensively in adult mouse tissues and during murine embryonic development. It was identified to be significantly stimulated by RA in murine palatal shelves at embryonic day 12 and in palatal mesenchymal cells in vitro. We demonstrate that MCPR1 protein was localized primarily in the cytoplasm and could be synthesized and secreted by transfected COS-7 cells. Both the secretory and recombinant proteins of Mcpr1 inhibited proliferation of murine embryonic palatal mesenchymal cells and impeded the progression from the G1 to S phase in the cell cycle. The cells were prone to apoptosis after exposure to glutathione S-transferase-MCPR1. Furthermore, knockdown of MCPR1 protein levels by antisense oligodeoxynucleotides promoted progression of cells from the G1 to S phase and completely abolished the RA-induced block of the cell cycle from the G1 to S phase. These findings suggest that Mcpr1 might function as one of the RA-up-regulated genes involved in inhibiting cell proliferation during palatogenesis and RA-induced cleft palate by regulating proliferation and apoptosis of embryonic palatal mesenchymal cells and might even play a role in the development of many other organs.
Journal of Biological Chemistry 12/2006; 281(45):33997-4008. · 4.77 Impact Factor
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ABSTRACT: In this paper, we propose an algorithm for maintaining the frequent itemsets discovered in a database with minimal re-computation
when new transactions are added to or old transactions are removed from the transaction database. An efficient algorithm called
EFPIM (Extending FP-tree for Incremental Mining), is designed based on EFP-tree (extended FP-tree) structures. An important
feature of our algorithm is that it requires no scan of the original database, and the new EFP-tree structure of the updated
database can be obtained directly from the EFP-tree of the original database. We give two versions of EFPIM algorithm, called
EFPIM1 (an easy vision to implement) and EFPIM2 (a fast algorithm), they both mining frequent itemsets of the updated database
based on EFP-tree. Experimental results show that EFPIM outperforms the existing algorithms in terms of the execution time.
07/2006: pages 56-63;
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ABSTRACT: The carotid body (CB) senses changes in arterial blood PO2 and modulates respiratory movement. It is generally accepted that the dopaminergic type I cells in the CB are chemoreceptors. However, it has not been clarified whether the carotid body has the ability to perceive the stimulation of proinflammatory cytokines. Interleukin 6 (IL-6) as a multifunctional cytokine plays a pivotal role in host defense mechanism. In the present study, we observed the expression of IL-6Ralpha mRNA and protein in the carotid body using immunohistochemistry, Western blots, and in situ hybridization. The results confirmed the presence of IL-6Ralpha proteins and mRNAs in the glomus cells of rat carotid body. These results suggest that the function of the carotid body may be influenced by the proinflammatrory cytokines through their receptors.
The Anatomical Record Part A Discoveries in Molecular Cellular and Evolutionary Biology 04/2006; 288(3):292-6.
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ABSTRACT: Cloning and screening a novel candidate gene related to developing mouse cleft palate.
The differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases.
Four novel express sequence tags were obtained, one of which spanning 809 bp was the full-length of the novel gene cDNA identified by Northern hybridization.
A novel candidate gene related to mouse cleft palate was cloned.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 11/2005; 22(5):481-4.
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Advances in Web-Age Information Management, 6th International Conference, WAIM 2005, Hangzhou, China, October 11-13, 2005, Proceedings; 01/2005
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ABSTRACT: To study the treatment technique for harvesting injury of donor blood vessels for the clinic application.
The data of 32 renal transplantation patients with injury of graft blood vessels were retrospectively reviewed. 60 renal transplantation patients with non-injury during the same term were selected as the control group. The treatment techniques for harvesting injury of graft blood vessels mainly includes end-to-end anastomosis of graft artery, side-to-side anastomosis of branch artery, end-to-side anastomosis of branch artery to the main renal artery, reconstruction of multiple segmental arteries by using iliac arterial grafts from cadaveric donors or recipients on the workbench, repairs of injuries for the smaller segmental/polar arteries by using inferior epigastric artery, end-to-end anastomosis of the lower thick segmental/polar arteries with the iliac internal arterial by placing kidney upside down.
Those injured included 28 arterial and 4 venous. Average bench surgery time was 42 minutes. Mean warm ischemic time was 31 minutes. No death occurred at an average follow-up of 3.5 years (1 - 5 years). There was no statistical difference in the 1-year graft survival, postoperative 1-year acute rejection, delayed graft function (DGF) and the incidence of constriction of vascular anastomosis rate (96.9%, 12.5%, 21.9%, 3.1%, respectively) compared with non-reconstructed kidneys during the same term (98.3%, 11.7%, 18.3%, 1.7%, P > 0.05, respectively).
The flexible and appropriate application of different vascular reconstruction means and satisfactory surgery techniques play an important role in assuring quality of kidney with harvesting blood vessels injury and donor kidney availability.
Zhonghua wai ke za zhi [Chinese journal of surgery] 06/2004; 42(10):607-10.
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ABSTRACT: To type the HLA-DR DNA for renal transplantation by PCR with sequence specific primers (PCR-SSP).
According to nucleotide sequences of HLA-DR, 16 pairs of specific primers and a pair of positive control primers were designed and synthesized for PCR-SSP. Then HLA-DR sites of 52 donors and recipients for renal transplantation were typed by the PCR-SSP.
All the samples were successfully typed by PCR-SSP with the synthesized primers. The results were available within 3 hours after sampling and the accuracy and reproducibility were 100%.
Genotyping for HLA-DR sites by PCR-SSP with primers reported herein was a simple and accurate technique suitable for clinical application.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 12/2003; 19(6):560-2.
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ABSTRACT: To study the pharmacokinetics of genistein in Beagle dogs.
Genistein, suspended in 0.5% CMC-Na solution, was orally administered to Beagle dogs at the dose of 5.34 mg.kg-1. At various time intervals, 1.5 mL of blood was drawn from the vein of dogs in their front legs. At the same time, urine and feces were collected. After the collection, the feces were homogenized with physiological saline (to 1 g feces, 10 mL physiological saline were added). The genistein in plasma, urine and homogenized feces was extracted twice by vortexing with 2.0 mL mixture of methyl tert-butyl ether and pentane (8:2). The organic phase was transferred into tubes and evaporated in ventilation cabinet. The residue was dissolved in 50 microL of methanol and 20 microL of the solution was drawn and detected by high-performance liquid chromatography. The pharmacokinetic parameter was calculated by 3P97 software.
The plasma concentration-time curve was fitted to a one-open-compartment model. The peak time was 0.29 h, and the elimination half-life was 0.52 h. After genistein was administered, 10.79% of genistein were excreted from urine and 21.55% from feces within 24 h. It was also found that 13.00% genistein were excreted from urine and 52.46% from feces within 60 h.
It showed that the speed of absorption and elimination of genistein was high in Beagle dog, and genistein was mainly excreted in the form of parent compound in urine and feces.
Yao xue xue bao = Acta pharmaceutica Sinica 10/2003; 38(9):646-9.
