Wei Gao

Jilin University, Yung-chi, Jilin Sheng, China

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Publications (108)287.18 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The spike (S) protein of porcine hemagglutinating encephalomyelitis virus (PHEV) may mediate infection by binding to a cellular neural cell adhesion molecule (NCAM). This study aimed to identify the crucial domain of the S1 subunit of the S protein that interacts with NCAM. Three truncated segments (S1-291, S277-794 and S548-868) of the S gene of PHEV and the NCAM gene were cloned individually into the Escherichia coli expression vectors and yeast two-hybrid expression vectors. The interaction between S1-291, S277-794, S548-868 and NCAM were detected by a GST pull-down experiment and yeast two-hybrid assay. Three fusion proteins (S1-291, S277-794 and S548-868) were screened for their interactions with NCAM by protein-protein interaction assays. The results of these assays clarified that S277-794 interacted with NCAM, while S1-291 and S548-868 did not. A small fragment (258-amino-acid fragment, residues 291-548) on the PHEV S protein was posited to be the minimum number of amino acids necessary to interact with NCAM. This fragment may be the receptor-binding domain that mediates PHEV binding to NCAM. © 2015 S. Karger AG, Basel.
    Intervirology 04/2015; 58(2):130-137. DOI:10.1159/000381060 · 1.77 Impact Factor
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    ABSTRACT: Two novel lanthanide metal organic frameworks [Eu4(bpt)4(DMF)2(H2O)6]•(DMF)5•(H2O)4 (1) and [Tb4(bpt)4(DMF)2(H2O)8]•(DMF)5•(H2O)3 (2) were synthesized by hydrothermal conditions, forming a three-dimensional structure ( H3bpt = biphenyl-3,4’,5-tricarboxylate ). Single crystal X-ray diffraction reveals that the compounds 1 and 2 are heteromorphic and have different topology due to the change of metal ions with radii and coordination steric hindrance. The unique luminescent properties of Eu3+ and Tb3+ ions motivated us to dope different amounts of Tb3+ into a single host to obtain light tunable materials. The tunable multicolor emissions, including red, red-orange, orange, yellow and green, can be readily achieved from the doped Ln-MOFs 3-6 by increasing the concentration of Tb3+. In addition, the compounds 5 and 6 also exhibits different temperature-dependent luminescent behaviors when the low concentration of Eu3+, generates the new Ln-MOFs thermometers.
    Dalton Transactions 04/2015; DOI:10.1039/C5DT00249D · 4.10 Impact Factor
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    ABSTRACT: The BIAN ligands in Brookhart catalysts were proved to be redox-active during the catalyst activation with alkylaluminum or MAO, and the neutral catalytically active species with a radical anionic BIAN rather than the cationic ones with a neutral BIAN ligand were confirmed to be formed in the catalytic system.
    Chemical Communications 03/2015; 51(32). DOI:10.1039/C5CC00582E · 6.72 Impact Factor
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    ABSTRACT: A series of new half-sandwich chromium(III) complexes Cp′[2-R1-6-(CHNR2)C6H2O]CrCl [R1 = iPr (1), tBu (2, 3, 4, 5); R2 = iPr (2, 4, 5), tBu (1, 3); Cp′ = C5H5(1, 2, 3), C5H2Ph3 (4), C5Me4Ph(5)] were synthesized from the reactions of Cp′CrCl2(THF) with corresponding salicyladiminato lithium. All complexes were characterized by elemental analyses and the structures of complexes 1, 2, 3 and 5 were determined by X-ray diffraction analysis. These complexes show moderate catalytic activities for ethylene polymerization upon activation with a relatively small amounts of AlMe3 or AlEt3, producing high molecular weight polyethylene in the form of spherical particles. Under similar conditions, the catalytic activity of complexes carrying a same salicylaldiminato ligand changes with the cyclopentadienyl ligand in the order Me5Cp > Me4PhCp > 1,2,4-Ph3Cp > Cp. The catalytic activity of these catalysts is also affected significantly by the AlR3 cocatalyst in the order AlMe3 > AlEt3.
    Journal of Organometallic Chemistry 03/2015; 779. DOI:10.1016/j.jorganchem.2014.12.020 · 2.30 Impact Factor
  • Polyhedron 11/2014; 83:236-241. DOI:10.1016/j.poly.2014.08.003 · 2.05 Impact Factor
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    ABSTRACT: We report a reversible phase transition in Mg doped lanthanum silicate La10Si5.8Mg0.2O26.8 at 16.1 GPa and room temperature. The phase transition reduces the symmetry from P6(3)/m to P6(3) and is attributed to a tilting of the Si/MgO4 tetrahedra. The high-pressure behavior of the sample is followed by in situ angle-dispersive X-ray diffraction in a diamond anvil cell. The high-pressure P6(3) phase exhibits a higher compressibility than the initial P6(3)/m structure. Furthermore, we observe that the transition pressure in the Mg doped system is elevated by similar to 1.6 GPa compared to the previously reported undoped La10Si6O27 system and that the initial compressibility in the P6(3)/m phase is lower. This behavior should be related to the larger size and less rigidness of MgO4 tetrahedra than SiO4 tetrahedra. The relevant mechanism is discussed in present paper.
    Journal of Alloys and Compounds 10/2014; 611:24–29. DOI:10.1016/j.jallcom.2014.05.122 · 2.73 Impact Factor
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    ABSTRACT: In this study, we investigated an acute outbreak of porcine hemagglutinating encephalomyelitis on a farm of 127 pigs in Jilin province, China. Porcine hemagglutinating encephalomyelitis virus (PHEV) was detected in suckling and weaning pigs by RT-PCR assays. Coronavirus-like particles were observed by electron microscopy. The virus isolate was designated PHEV-JT06. The clinical signs, nervous symptoms and positive labeling of neurons in the cerebral cortex with an immunohistochemical stain in PHEV-JT06-infected BALB/c mice supported the diagnosis of PHEV infection. The five full-length PHEV-JT06 structural genes were cloned, sequenced and analyzed. Phylogenetic studies based on the nucleotide and amino acid sequences of the five genes in the outbreak showed that PHEV remained genetically stable. PHEV shares 95.3-99.3 % amino acid sequence identity with American strains (AY078417), suggesting that the Chinese isolate is most likely derived from the North American strain. Additionally, PHEV, HCoV-OC43 and BCoV were genetically close. These results may provide some insights into the genotype of the etiological agent responsible for the porcine hemagglutinating encephalomyelitis outbreak and could also provide a comparative view of the genomics of the five structural proteins of PHEV.
    Archives of Virology 04/2014; 159(9). DOI:10.1007/s00705-014-2070-y · 2.28 Impact Factor
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    ABSTRACT: The adsorption of Rhodamine-B (RhB) onto treated rice husk-based activated carbon was investigated in this paper. The influences of initial RhB concentration, temperature and pH on dye adsorption were analyzed. Initial RhB concentration and temperature played important roles in this adsorption process, and pH had a little effect on RhB adsorption. Adsorption kinetics, equilibrium and thermodynamics study was carried out. Results demonstrated that pseudo-second order kinetic model represented the adsorption kinetics of RhB well. Adsorption equilibrium data were well described by Langmuir isotherm model. Thermodynamic parameters, including the Gibb's free energy change (Delta G), standard enthalpy change (Delta H) and standard entropy change (Delta S), revealed that this adsorption process was spontaneous and endothermic.
    Colloids and Surfaces A Physicochemical and Engineering Aspects 04/2014; 446:1–7. DOI:10.1016/j.colsurfa.2014.01.030 · 2.35 Impact Factor
  • Journal of Alloys and Compounds 02/2014; 586:279-284. DOI:10.1016/j.jallcom.2013.09.216 · 2.73 Impact Factor
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    ChemInform 01/2014; 45(1). DOI:10.1002/chin.201401033
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    ABSTRACT: Apatite-type La9.33Ge6O26 powders have been successfully synthesized by a facile molten-salt synthesis method, using NaCl as a eutectic salt. The morphology, structure and phase composition of the powders were examined. The results demonstrated that the apatite-type La9.33Ge6O26 powders are homogeneous, nano-size, non-agglomerated and well crystallized particles, which are favorable to obtain highly dense pellets. Compared with the conventional methods, dense pellets have been successfully sintered at a relatively low temperature of 1100 °C, which inhibits the vaporization of GeO2. The pellets exhibit a higher conductivity with a value of 2.4 × 10−2 S cm−1 at 850 °C, due to the high density and the avoidance of impurity La2GeO5. The main problems such as Ge volatility, agglomeration and crystallinity encountered in the conventional methods have been effectively solved via the molten-salt synthesis method. In addition, the related mechanism is also discussed in the present paper.
    RSC Advances 01/2014; 4(31):15968. DOI:10.1039/C4RA00713A · 3.71 Impact Factor
  • Journal of Organometallic Chemistry 01/2014; 749:350-355. DOI:10.1016/j.jorganchem.2013.10.020 · 2.30 Impact Factor
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    ABSTRACT: A series of tridentate anilido-imine [N,N,O] ligands 2-(2-(R12-2,6-C6H3NH)C6H4)HC═N(4-tBu-6-R2-C6H2OH) [R1 = Me, R2 = tBu (LaH2); R1 = Et, R2 = tBu (LbH2); R1 = iPr, R2 = tBu (LcH2); R1 = Me, R2 = Ad (LdH2); R1 = Et, R2 = Ad (LeH2); R1 = iPr, R2 = Ad (LfH2)] were synthesized and characterized. Reaction of the free ligand LdH2 with TiCl4 in toluene at low temperature affords the zwitterionic complex Ld+H2Ti–Cl4 (1d). The zwitterionic complex can be dissolved in THF to form THF-solvated complex 1d′. Heating 1d in toluene at 40 °C affords the neutral complex LdHTiCl3 (2d) by losing a HCl. Complex 2d can be fully converted to the final complex LdTiCl2 (3d) at 140 °C under vacuum by losing another HCl. Complexes 3a–3c, 3e, and 3f were also synthesized in high yields in the same one-pot procedure. All complexes were characterized by 1H and 13C NMR spectroscopy, and the molecular structures of 1d′, 3b, 3d, and 3e were determined by single-crystal X-ray diffraction analysis. The titanium centers in complexes 3b, 3d, and 3e are five-coordinated with a geometry situated between trigonal bipyramid and square pyramid. Upon activation with alkylaluminum and Ph3C+B(C6F5)4–, complexes 3a–3f exhibit moderate catalytic activity for ethylene polymerization.
    Organometallics 10/2013; 32(21):6277–6285. DOI:10.1021/om400562p · 4.25 Impact Factor
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    ABSTRACT: Apatite-type lanthanum silicate (ATLS) electrolyte coatings for use in intermediate-temperature solid oxide fuel cells were deposited by atmospheric plasma spraying (APS). Plasma-sprayed coatings with typical composition La10(SiO4)6O3 exhibiting good densification and high oxide ionic conductivity were obtained by properly adjusting the spraying parameters, particularly the gun current. The highest obtained ionic conductivity value of 3.3 mS/cm at 1,173 K in air is comparable to other ATLS conductors. This work demonstrated empirically that utilization of the APS technique is feasible to synthesize dense La10(SiO4)6O3 electrolyte coatings using gun currents within an unusually broad range.
    Journal of Thermal Spray Technology 10/2013; 22(7). DOI:10.1007/s11666-013-9950-7 · 1.49 Impact Factor
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    ABSTRACT: Reduction reactions of the phenanthrene-o-aryliminoquinone with 0.5 equiv of magnesium in tetrahydrofuran afford the bisligated magnesium complexes [(Ar)piq](2)Mg(thf) (1a, Ar = 2,6-Me2C6H3; 1b, Ar = 2,6-(Pr2C6H3)-Pr-i). 1a can be further reduced by magnesium to oxygen-bridged dinuclear complex [(mu-O)(Ar-)piqMg(thf)(2)](2) (2). Similar reduction reactions of the N,N-bis(arylimino)phenanthrene with magnesium give bis(arylimino)phenanthrene magnesium complexes (Ar1,Ar2)bipMg(thf)(3) (3, Ar-1 = Ar-2 = 2,6-Me2C6H3: 4, Ar-1 = Ar-2 = 2,6-(Pr2C6H3)-Pr-i: 5, Ar-1 = 2,6-Me2C6H3, Ar-2 = 2,6-(Pr2C6H3)-Pr-i). All the complexes were well characterized and the molecular structures of 1b, 2, 3, and 5 were established by X-ray diffraction analysis.
    Polyhedron 10/2013; 63:91-95. DOI:10.1016/j.poly.2013.07.010 · 2.05 Impact Factor
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    ABSTRACT: Chiral bis(salicylaldimine) ligands derived from binaphthol (LH2) were synthesized by condensation of (R/S) 2,2′-dihydroxy-1,1′-binaphthyl-3,3′-dicarbaldehyde with 2-anisidine. Cobalt and nickel complexes (CoL)2(OAc)2Co (1) and (NiL)2(OAc)2Ni (2) were synthesized via reactions of the ligand with the corresponding metal acetate salt. Both complexes were characterized by elemental analysis, IR, MS, and single-crystal X-ray diffraction analysis. The X-ray analysis reveals linear trinuclear for 1 and 2 and the metal ions in both complexes are octahedral coordination. The two acetates separately bridge the center metal with one of the terminal metals in M–O–C–O–M manner. The magnetic susceptibility of 1 below 150 K suggests the existence of a weak ferromagnetic exchange at low temperatures, while antiferromagnetic interactions among Co(II) cores were observed above 150 K. Complex 2 shows similar magnetic behavior to that of 1.
    Journal of Coordination Chemistry 09/2013; 66(18):3182-3192. DOI:10.1080/00958972.2013.830716 · 2.22 Impact Factor
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    ABSTRACT: A series of NCO/NCS pincer precursors, 3-(Ar(2) OCH2 )-2-Br-(Ar(1) NCH)C6 H3 (((Ar1) NCO(Ar2) )Br, 3 a-3 d) and 3-(2,6-Me2 C6 H3 SCH2 )-2-Br-(Ar(1) NCH)C6 H3 (((Ar1) NCS(Me) )Br, 4 a and 4 b) were synthesized and characterized. The reactions of [(Ar1) NCO(Ar2) ]Br/ [(Ar1) NCS(Me) ]Br with nBuLi and the subsequent addition of the rare-earth-metal chlorides afforded their corresponding rare-earth-metal-pincer complexes, that is, [((Ar1) NCO(Ar2) )YCl2 (thf)2 ] (5 a-5 d), [((Ar1) NCO(Ar2) )LuCl2 (thf)2 ] (6 a, 6 d), [((Ar1) NCO(Ar2) )GdCl2 (thf)2 ] (7), [{((Ar1) NCS(Me) )Y(μ-Cl)}2 {(μ-Cl)Li(thf)2 (μ-Cl)}2 ] (8, 9), and [{((Ar1) NCS(Me) )Gd(μ-Cl)}2 {(μ-Cl)Li(thf)2 (μ-Cl)}2 ] (10, 11). These diamagnetic complexes were characterized by (1) H and (13) C NMR spectroscopy and the molecular structures of compounds 5 a, 6 a, 7, and 10 were well-established by X-ray diffraction analysis. In compounds 5 a, 6 a, and 7, all of the metal centers adopted distorted pentagonal bipyramidal geometries with the NCO donors and two oxygen atoms from the coordinated THF molecules in equatorial positions and the two chlorine atoms in apical positions. Complex 10 is a dimer in which the two equal moieties are linked by two chlorine atoms and two ClLiCl bridges. In each part, the gadolinium atom adopts a distorted pentagonal bipyramidal geometry. Activated with alkylaluminum and borate, the gadolinium and yttrium complexes showed various activities towards the polymerization of isoprene, thereby affording highly cis-1,4-selective polyisoprene, whilst the NCOlutetium complexes were inert under the same conditions.
    Chemistry - An Asian Journal 09/2013; 8(9). DOI:10.1002/asia.201300581 · 3.94 Impact Factor
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    ABSTRACT: A series of poly(ethylene terephthalate) (PET) nanocomposites were prepared by in situ polymerization containing different amounts of surface treated calcium carbonate (CaCO3). As verified by SEM micrographs, the dispersion of CaCO3 nanoparticles into the PET matrix was homogeneous when the content of CaCO3 was below 5 wt.%, however, some aggregates existed at higher filler content. DSC study results showed that the crystallization became faster, and there was, also, a shifting of temperature of the crystallization peak to higher value by the addition of CaCO3 till 5 wt.%, this being evidence that CaCO3 can act as nucleating agent. From TG curves it was concluded that PET and the samples with different content of CaCO3 nanoparticles presented good thermal stabilities, since no remarkable mass loss occurred up to 360 °C (< 0.5%). Moreover, from the variations of the activation energies, calculated from the TG curves, it was clear that nanocomposites containing CaCO3 had a higher activation energy compared to the pure PET, indicating that the addition of the nanoparticles could slightly increased the thermal stability of the matrix.
    Powder Technology 08/2013; 244:45–51. DOI:10.1016/j.powtec.2013.04.008 · 2.27 Impact Factor
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    ABSTRACT: A series of new half-sandwich titanium(IV) complexes che late d w ith 2 -(tetrame thylcyclope nt adie ny l)-benzylamido ligands, 2-Me 4 CpC 6 H 4 CH 2 (R)NTiCl 2 (R = i Pr ( 1 ), Cy ( 2 ), n Pr (3 ), 4-MePh ( 4 )), have been synthesized from the chlorotrimethylsilane elimination reactions of TiCl 4 with the double-trimethylsilyl-substituted preligands 2-Me 4 (TMS)-CpC 6 H 4 CH 2 (R)N(TMS) (R = i Pr (TMS 2 L1), Cy (TMS 2 L2), n Pr (TMS 2 L3), 4-MePh (TMS 2 L4)). The free ligands H 2 L1− H 2 L4were synthesized by reduction of the corresponding imine compounds 2-Me 4 CpHC 6 H 4 CHNR with LiAlH 4 , while the imine compounds were formed in situ by a condensation reaction of 2-tetramethylcyclopentadienylbenzaldehyde with the corresponding amine. The free ligands were characterized by LC-MS and 1 H NMR spectroscopy, and the titanium complexes were characterized by 1 H and 13 C NMR, elemental analyses, and single-crystal X-ray crystallography. The X-ray crystallography analysis reveals that these titanium complexes possess a three-legged piano-stool geometry with the amide N atom in a mitered six-membered chelating ring and the two chloride atoms as the legs. The angle between the cyclopentadienyl plane and the attached phenyl plane in these complexes (59.2, 62.7, and 61.9 ° for complexes 1, 2, and 4 , respectively) is much less than 90 ° in the solid state. Upon activation with Al i Bu 3 and Ph 3 CB(C 6 F 5 ) 4 , complexes 1− 4 exhibit reasonable catalytic activity for ethylene homopolymerization and copolymerization with 1-hexene at 110 ° C, producing high-molecular-weight polyethylenes and poly(ethylene- co -1-hexenes) with relatively high comonomer incorporation. Complex 4 was found to show higher catalytic activity for ethylene/1-hexene copolymerization than complexes 1−3 under similar conditions, while complexes 1− 3 produce poly(ethylene- co -1-hexenes) with higher comonomer incorporation.
    Organometallics 07/2013; 32(15):4185. DOI:10.1021/om4003945 · 4.25 Impact Factor
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    ABSTRACT: Porcine hemagglutinating encephalomyelitis is an acute, highly contagious disease in piglets that is caused by the porcine hemagglutinating encephalomyelitis virus (PHEV). However, the pathogenesis of PHEV and the relationship between PHEV and the host cells are not fully understood. In this study, we investigated whether the PHEV-induced cytopathic effect (CPE) was caused by apoptosis. Replication of PHEV in a porcine kidney-derived cell line (PK-15 cells) caused an extensive CPE, leading to the destruction of the entire monolayer and the death of the infected cells. Staining with Hoechst 33342 revealed morphological changes in the nuclei and chromatin fragmentation. In addition, PHEV caused DNA fragmentation detectable by agarose gel electrophoresis 48h post-infection, increasing with the incubation time. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 96h post-infection, as determined using flow cytometry and fluorescence microscopy of cells that were stained with annexin V-FITC and propidium iodide (PI). Moreover, as is commonly observed for coronavirus infections of other animals, the activities of the effecter caspase, caspase-3, and the initiator caspases, caspase-8 and caspase-9, which are representative factors in the death receptor-mediated apoptotic pathway and the mitochondrial apoptotic pathway, respectively, were increased in PHEV-infected PK-15 cells. Moreover, the tripeptide pan-ICE (caspase) inhibitor Z-VAD-FMK blocked PHEV-induced apoptosis but did not have an effect on virus production by 96h post-infection. These results suggested that PHEV induces apoptosis in PK-15 cells via a caspase-dependent pathway. Apoptotic death of infected cells is detrimental to animals because it causes cell and tissue destruction. Although the pathological characteristics of PHEV are largely unknown, apoptosis may be the pathological basis of the lesions resulting from PHEV infection.
    Virus Research 06/2013; 176(1-2). DOI:10.1016/j.virusres.2013.05.019 · 2.83 Impact Factor

Publication Stats

756 Citations
287.18 Total Impact Points

Institutions

  • 2001–2015
    • Jilin University
      • • State Key Laboratory of Supramolecular Structure and Materials
      • • State Key Lab of Superhard Materials
      • • Department of Chemistry
      Yung-chi, Jilin Sheng, China
  • 2014
    • Heilongjiang Institute of Science and Technology
      Charbin, Heilongjiang Sheng, China
  • 2012
    • General Hospital of Shenyang Military Region
      Feng-t’ien, Liaoning, China
  • 2007–2009
    • Université de Technologie de Belfort-Montbéliard
      • Laboratoire d’Etudes et de Recherches sur les Matériaux, les Procédés et les Surfaces (LERMPS)
      Belfort, Franche-Comté, France
  • 2008
    • Chinese Academy of Sciences
      • Department of Chemistry
      Peping, Beijing, China