W-J Xiao

Publications (4)11.07 Total impact

• Article: Effect of tea catechins on regulation of cell proliferation and antioxidant enzyme expression in H(2) O(2) -induced primary hepatocytes of goat in vitro.

  R Z Zhong, W J Xiao, D W Zhou, C Y Tan, Z L Tan, X F Han, C S Zhou, S X Tang
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  ABSTRACT: Tea catechins (TC) are polyphenols that have potent antioxidant activity. The objectives of this study were to determine the effects of TC on antioxidant status of hepatocytes challenged with H(2) O(2) . Primary hepatocytes of goat were exposed to 1 mm H(2) O(2) without or with 5, 50 and 500 μg/ml TC. The cells were harvested at 48 h post-treatment to determine effects of TC on proliferation, apoptotic features and membrane integrity of cells, and expression of genes and activities of antioxidant enzymes. H(2) O(2) exposure caused damage to cells (p < 0.001). A lower concentration of TC (5 μg/ml) displayed a protective effect by inhibiting exorbitant cell proliferation and DNA degradation. Both H(2) O(2) exposure and TC pre-incubation affected expression of antioxidant enzymes at mRNA and protein levels (p < 0.001). The activities of catalase (CAT) (p = 0.027), CuZn-superoxide dismutase (CuZn-SOD) (p < 0.001) and glutathione peroxidase (GPx) (p < 0.001) increased with TC pre-incubation followed by H(2) O(2) challenge. Changes of CuZn-SOD activity induced by H(2) O(2) and TC basically paralleled the changes in the corresponding mRNA and protein levels, but the correlation in CAT and GPx expression displayed slightly different patterns at different concentrations of TC. These findings infer that oxidative stress can induce deleterious cellular responses and this unfavourable condition may be alleviated by treatment with TC.
  J Anim Physiol a Anim Nutr 03/2012; · 0.86 Impact Factor
• Article: Polymorphisms in the human ALOX12 and ALOX15 genes are associated with peak bone mineral density in Chinese nuclear families.

  W-J Xiao, Y-H Ke, J-W He, H Zhang, J-B Yu, W-W Hu, J-M Gu, G Gao, H Yue, C Wang, Y-Q Hu, M Li, Y-J Liu, W-Z Fu, Z-L Zhang
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  ABSTRACT: Association between ten single-nucleotide polymorphisms (SNPs) in the human ALOX12 and ALOX15 genes and variations in peak bone mineral density (BMD) in a large sample of Chinese nuclear families with female offspring using the quantitative transmission disequilibrium test (QTDT). Our results suggest that the genetic polymorphisms in both human ALOX12 and ALOX15 may contribute to variations in the peak BMD of Chinese women. The aim of this study was to investigate whether polymorphisms in the human ALOX12 and ALOX15 genes are associated with variations in peak BMD in Chinese nuclear families with female offspring. Each five SNPs in the ALOX12 and ALOX15 genes were genotyped in a total of 1,260 individuals from 401 Chinese nuclear families. The BMD of the lumbar spine, femoral neck and total hip was measured by dual-energy X-ray absorptiometry. We tested whether a single SNP or a haplotype was associated with peak BMD variations using the QTDT. Using QTDT to measure within-family associations in ALOX15, we observed a significant association between rs916055 and BMD in the lumbar spine (p = 0.027 in the permutation 1,000 test). However, in ALOX12, rs312470 was significantly associated with BMD in the femoral neck (p = 0.029 and p = 0.036 in the permutation 1,000 test). The results of a haplotype analysis supported the findings of the single locus test for ALOX15. Our results suggest that the genetic polymorphisms in both human ALOX12 and ALOX15 may contribute to variations in the peak BMD of Chinese women.
  Osteoporosis International 11/2011; 23(7):1889-97. · 4.58 Impact Factor
• Source

  Available from: PubMed Central

  Article: ALOX12 polymorphisms are associated with fat mass but not peak bone mineral density in Chinese nuclear families.

  W-J Xiao, J-W He, H Zhang, W-W Hu, J-M Gu, H Yue, G Gao, J-B Yu, C Wang, Y-H Ke, W-Z Fu, Z-L Zhang
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  ABSTRACT: Arachidonate 12-lipoxygenase (ALOX12) is a member of the lipoxygenase superfamily, which catalyzes the incorporation of molecular oxygen into polyunsaturated fatty acids. The products of ALOX12 reactions serve as endogenous ligands for peroxisome proliferator-activated receptor γ (PPARG). The activation of the PPARG pathway in marrow-derived mesenchymal progenitors stimulates adipogenesis and inhibits osteoblastogenesis. Our objective was to determine whether polymorphisms in the ALOX12 gene were associated with variations in peak bone mineral density (BMD) and obesity phenotypes in young Chinese men. All six tagging single-nucleotide polymorphisms (SNPs) in the ALOX12 gene were genotyped in a total of 1215 subjects from 400 Chinese nuclear families by allele-specific polymerase chain reaction. The BMD at the lumbar spine and hip, total fat mass (TFM) and total lean mass (TLM) were measured using dual-energy X-ray absorptiometry. The pairwise linkage disequilibrium among SNPs was measured, and the haplotype blocks were inferred. Both the individual SNP markers and the haplotypes were tested for an association with the peak BMD, body mass index, TFM, TLM and percentage fat mass (PFM) using the quantitative transmission disequilibrium test (QTDT). Using the QTDT, significant within-family association was found between the rs2073438 polymorphism in the ALOX12 gene and the TFM and PFM (P=0.007 and 0.012, respectively). Haplotype analyses were combined with our individual SNP results and remained significant even after correction for multiple testing. However, we failed to find significant within-family associations between ALOX12 SNPs and the BMD at any bone site in young Chinese men. Our present results suggest that the rs2073438 polymorphism of ALOX12 contributes to the variation of obesity phenotypes in young Chinese men, although we failed to replicate the association with the peak BMD variation in this sample. Further independent studies are needed to confirm our findings.
  International journal of obesity (2005) 03/2011; 35(3):378-86. · 4.34 Impact Factor
• Article: Single nucleotide polymorphisms and haplotypes of TLR6 gene in Chinese Cantonese population.

  W J Xiao, Y X Gong, Z H Liu, J M Wang
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  ABSTRACT: Sequence polymorphisms in the coding region of Toll-like receptor 6 gene were investigated in Chinese Cantonese population. By amplifying and sequencing a 2787 bp segment containing the entire coding region of TLR6 gene of 191 individuals in Chinese Cantonese population, a total of seven single nucleotide polymorphisms (SNP) along with their frequencies were detected. Comparing these data with SNP published in dbSNP database of National Center for Biotechnology Information (NCBI), two SNP (+176T/C and +1408G/T) were firstly reported, and five SNP caused amino-acid substitution. Sixteen haplotypes and their distributions were reconstructed. Linkage disequilibrium analysis and neutrality test were also performed. Comparing with other ethnic populations, Chinese Cantonese displayed obvious differences in TLR6 polymorphism. It may in part reflect the ethnic diversity of pathogen susceptibility and facilitate to develop the disease-association studies as well as population genetics and evolutionary research.
  International Journal of Immunogenetics 08/2010; 37(4):245-51. · 1.29 Impact Factor