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ABSTRACT: The levels of magnesium (Mg), zinc (Zn) and copper (Cu) in the plasma, erythrocytes (RBC) and polymorphonuclear (PMN) and lymphocyte (L), leukocytes (WBC) of 45 diabetic children were compared to those of 12 normal children and were related to the diabetic control via HbA1 and fasting blood sugar (FBS) assessments. Mineral levels were determined via Zeeman-effect atomic absorption spectrophotometry following separation of plasma, RBC, and WBC fractions (PMN vs. M). ANOVA (four-way, blood components, by two-way, diabetic vs. normal children) was significant for Mg only (F = 4.60, P < 0.004). Plasma Mg and M-WBC Mg were significantly lower in children with diabetes (780 +/- 16 vs. 860 +/- 29 mumol/l and 519 +/- 33 vs. 866 +/- 86 micrograms/10(10) cells, respectively). RBC Zn was significantly lower in diabetic youngsters by t-test (0.48 +/- 0.012 vs. 0.57 +/- 0.046 per mumol/g Hgb, t = 2.79, P < 0.004), but the ANOVA for Zn was not significant. Cu level differences were not significant. HbA1 was predicted only by PMN-WBC Mg (F = 8.78, P < 0.04) and FBS by none. In conclusion, the mineral status of these diabetic children was altered in regard to Mg, but was mainly independent of diabetic control.
Clinica Chimica Acta 05/1993; 215(1):21-8. · 2.54 Impact Factor
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ABSTRACT: To determine whether a reduction of dietary lactose affects mineral absorption in preterm infants, a controlled, randomized, 72-hour balance study was conducted with very low birth weight infants (birth weights less than 1400 gm) fed Similac Special Care formula with a 50:50 carbohydrate blend of glucose polymers and lactose (n = 8) or a nearly identical formula with 100% carbohydrate as lactose (n = 10). The studies were conducted after at least 3 consecutive days of full oral feeds at a mean age of approximately 23 days. There was no difference between groups in percentage of apparent absorption of calcium, phosphorus, magnesium, zinc, copper, or manganese. Retention of these nutrients was similar to or greater than that which occurs in utero. Calcium absorption was 75% of intake. These results indicate that reducing the lactose content of premature-infant formulas to 50% of the carbohydrate does not impair mineral absorption.
Journal of Pediatrics 09/1990; 117(2 Pt 1):283-7. · 4.11 Impact Factor
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ABSTRACT: Acrosin, a neutral proteinase, is located within the acrosome. The aim of this study was to evaluate acrosin concentrations in patients with severe damage of the sperm head and to determine whether acrosin concentration could predict the chances of fertilization in an IVF program. Sixty patients were accepted into this study, prospectively. The patients were divided into two groups, those with a normal morphology of less than 14% (group I, n = 33) and those with normal morphology less than 14% (group II, n = 27). All patients had a sperm concentration of less than 20 million sperm/ml and less than 30% progressively motile sperm. The acrosin assays were performed on the semen sample obtained on the day of IVF. Routine IVF insemination procedures were used, and only mature oocytes were considered. The only factor that showed a significant correlation of fertilization was normal morphology (p less that 0.01). The mean acrosin level was 73.4 /+- 38.6 mED/10 million sperm in group I and 70.9 /+- 42.7 mIU/10 million sperm in group II (no significant difference). The fertilization rate in group I was 45.4% and in group II, 77.7% p less than 0.002). Acrosin levels were not significantly different in patients with and without fertilization (72.0 /+- 42.1 and 73.6 /+- mIU/10 million sperm, respectively).
Archives of Andrology 02/1988; 20(3):237-41. · 0.89 Impact Factor
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Annals of the New York Academy of Sciences 02/1988; 541:297-309. · 3.15 Impact Factor
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ABSTRACT: Nonsteroidal biochemical markers of oocyte maturity could presumably aid in oocyte selection and in the timing of insemination for in vitro fertilization. We assessed the usefulness of six potential markers found in follicular fluid. We used kinetic immunonephelometry to measure concentrations of alpha 1-antitrypsin, alpha 2-macroglobulin, antithrombin III, ceruloplasmin, fibrinogen, and plasminogen in 53 fluids and the corresponding plasma from 20 women undergoing in vitro fertilization. Specimens were obtained from both mature and immature follicles from each woman. The respective protein concentrations in follicular fluid could be assayed with a between-assay CV of 1.5% to 3.2%. Analytical-recovery studies indicated that only fibrinogen and antithrombin III concentrations were altered (by 120% and 75%, respectively) during aspiration of the follicle into saline. All protein concentrations were significantly increased in mature follicles, as were the follicular fluid:plasma concentration ratios for alpha 1-antitrypsin, antithrombin III, and ceruloplasmin.
Clinical Chemistry 08/1987; 33(7):1185-9. · 7.91 Impact Factor
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Urology 08/1986; 28(1):1-9. · 2.43 Impact Factor
