Vikas Yadav Patade

Bhabha Atomic Research Centre, Mumbai, State of Maharashtra, India

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Publications (28)39.56 Total impact

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    ABSTRACT: We report elevated biomass and altered growth characteristics of tobacco plants up on transformation with a NAC (NAM, ATAF1/2,CUC2) gene (GenBank Accession FJ754254) isolated from Lepidium latifolium L. (LlaNAC). Transgenic plants showed significant differences in fresh weight, midrib length of longest leaf, leaf area, height of the plant, root and shoot weights, etc. during vegetative phase. On 100th day after sowing (DAS), plants of transgenic lines were 2-3 times taller than the wild type plants, though no significant difference was recorded in moisture contents of any of the plant tissues. Over-expression of NAC gene up to 2,000 fold was recorded in leaves of transgenic plants on 100th DAS. Interestingly, transgenic plants showed significantly shortened (P(t) = 0.02-0.04) life cycle, as they showed a completely altered growth behaviour. Transgenic plants entered reproductive phase earlier by 60 days, with lines NC2 and NC7b entering first, followed by line NC10. However, the time period spent in the reproductive phase by the plant was nearly twice in case of transgenic lines NC2, NC7b and NC10, as compared to the wild type plants. Despite that, these lines completed their life cycle in 45-60 days lesser than the time taken by wild-type tobacco plants. No difference was recorded in fruit and seed yield of transgenic or wild type plants. To the best of our knowledge, this is the first report on over-expression of NAC gene causing altered growth and biomass patterns. We expect this study to become an important reference towards future engineering of plants for fuel and fodder purposes.
    Molecular Biology Reports 08/2014; · 2.51 Impact Factor
  • 07/2014: pages 345-374; , ISBN: 978-90-8686-244-3
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    ABSTRACT: Antioxidant defense system provides protection against oxidative damage caused by abiotic stresses including salinity. Ameliorative effects of l-proline, l-glutamine, glycine betaine (GB) on growth, proline accumulation and antioxidant enzyme activities were studied using cultured cells of sugarcane against salt (NaCl) stress. NaCl stress reduced growth rate significantly over the control however, proline or glutamine supplementation resulted in growth revival. Proline supplementation to media with or without salt increased accumulation of free proline significantly than the controls and other (proline, GB and glutamine) treatments. Salt stress led to increase in superoxide dismutase (SOD) and glutathione reductase activity whereas guaiacol peroxidase (GPX), catalase and ascorbate peroxidase activities were significantly suppressed. Proline supplementation to the salt medium improved the GPX activity over the salt media supplemented with glutamine or glycine betaine. The activity ratio between SOD and H2O2 scavenging enzyme activities, which is considered as a working hypothesis for biochemical marker for salt tolerance, was lower in salt medium supplemented with proline. Thus, the higher growth rate and the lower activity ratio suggest maximum salt stress ameliorative potential of proline in sugarcane cultured cells.
    Sugar Tech 03/2014; 16(1). · 0.50 Impact Factor
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    ABSTRACT: Curcin, a type I ribosomal inhibiting protein-RIP, encoded by curcin precursor gene, is a phytotoxin present in Jatropha (Jatropha curcas L.). Here, we report designing of RNAi construct for the curcin precursor gene and further its genetic transformation of Jatropha to reduce its transcript expression. Curcin precursor gene was first cloned from Jatropha strain DARL-2 and part of the gene sequence was cloned in sense and antisense orientation separated by an intron sequence in plant expression binary vector pRI101 AN. The construction of the RNAi vector was confirmed by double digestion and nucleotide sequencing. The vector was then mobilized into Agrobacterium tumefaciens strain GV 3101 and used for tissue culture independent in planta transformation protocol optimized for Jatropha. Germinating seeds were injured with a needle before infection with Agrobacterium and then transferred to sterilized sand medium. The seedlings were grown for 90 days and genomic DNA was isolated from leaves for transgenic confirmation based on real time PCR with NPT II specific dual labeled probe. Result of the transgenic confirmation analysis revealed presence of the gene silencing construct in ten out of 30 tested seedlings. Further, quantitative transcript expression analysis of the curcin precursor gene revealed reduction in the transcript abundance by more than 98 % to undetectable level. The transgenic plants are being grown in containment for further studies on reduction in curcin protein content in Jatropha seeds.
    Molecular Biology Reports 02/2014; · 2.51 Impact Factor
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    ABSTRACT: Our containment trials have established cold tolerance in Nicotiana tabacum osmotin (Nt Osm) transgenic tomato (Solanum lycopersicum L. cv. Pusa Ruby). Though, the stress tolerance mechanisms have been studied at physio-biochemical levels, molecular mechanisms underlying the tolerant response are still not well studied. Therefore, quantitative transcript expression of Osmotin and other stress responsive genes (CBF1, P5CS and APX) was studied in response to cold (4°C; 2 and 24 h) treatment in the transgenic and wild type tomato plants. The expression analysis revealed differential transcript regulation in the transgenic and wild type plants on the cold exposure. In general, the genes were either earlier induced or the extent of fold change in transcript expression over the respective untreated controls was higher in transgenic than in the wild type plants on cold exposure. The transcript expression data also supported the metabolite analysis on free Proline and ascorbate content. The results thus suggest that constitutive over expression of the Osmotin modulate transcript abundance and functional expression products of the other stress responsive genes thereby, imparting cold tolerance in the transgenic tomato plants.
    SpringerPlus 12/2013; 2(1):117.
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    ABSTRACT: Glycerol-3-phosphate acyltransferase (GPAT) catalyzes first and the rate limiting step in glycerolipid synthesis pathway, which in turn contribute to stabilization of plasma membrane structure and oil lipid synthesis in plant cells. Here, we report cloning and characterization of GPAT gene from Lepidium latifolium (LlaGPAT). The cDNA sequence (1,615 bp) of LlaGPAT gene consisted of 1,113 bp ORF encoding a protein of 370 aa residues, with deduced mass of 41.2 kDa and four acyltransferase (AT) motifs having role in catalysis and in glycerol-3-phosphate binding. Southern blot analysis suggested presence of a single copy of the gene in the genome. Tissue specific expression of the gene was seen more abundantly in aerial parts, compared to the roots. Quantitative real-time PCR indicated down-regulation of the gene by cold (4 °C), drought (PEG6000), salt (300 mM NaCl) and ABA (100 μM) treatments. Considering the vitality of the function of encoded enzyme, LlaGPAT can be considered a potential candidate gene for genetic engineering of oil yields and abiotic stress management in food as well as fuel crops.
    Molecular Biology Reports 05/2013; · 2.51 Impact Factor
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    ABSTRACT: We have isolated and in silico characterized a cold-induced LlaDREB1b encoding a putative DRE-binding transcription factor from Lepidium latifolium. Its cDNA (JN214345) sequence (998 bp) consisted of a 642 bp ORF, 168 and 188 bp of 5' and 3' UTR regions, respectively, encoding a protein of 213 aa with deduced molecular mass 23.85 kDa and pI of 4.63. In silico and phylogenetic analysis further suggested that the protein showed features of a typical member of the AP2/EREBP family of DNA-binding proteins. Southern blot analysis indicated that multiple copies of the gene could be present in the genome. Its transcripts were abundant in leaves, petiole and stem, but scarce in roots and could strongly be induced by cold treatment (4 °C), weakly by drought and salt stress, and did not respond to ABA treatment. Thus, LlaDREB1b is a potential candidate for abiotic stress-tolerance engineering in crop plants upon its further functional validation.
    Molecular Biology Reports 12/2012; · 2.51 Impact Factor
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    ABSTRACT: Benefits of seed priming in seedling establishment and tolerance to subsequent stress exposure are well reported. However, the molecular mechanisms underlying the priming mediated benefits are not much discovered. Results of our earlier experiments established that thiourea (TU) seed priming imparts cold tolerance to capsicum seedlings. Therefore, to understand molecular mechanisms underlying priming mediated cold stress tolerance, quantitative transcript expression of stress responsive genes involved in transcript regulation (CaCBF1A, CaCBF1B, Zinc Finger protein, CaWRKY30), osmotic adjustment (PROX1, P5CS, Osmotin), antioxidant defence (CAT2, APX, GST, GR1, Cu/Zn SOD, Mn SOD, Fe SOD), signaling (Annexin), movement of solutes and water (CaPIP1), and metabolite biosynthesis through phenylpropanoid pathway (CAH) was studied in response to cold (4 °C; 4 and 24 h) stress in seedlings grown from the TU primed, hydroprimed and unsoaked seeds. The transcript expression of CaWRKY30, PROX1, Osmotin, Cu/Zn SOD and CAH genes was either higher or induced earlier on cold exposure in thiourea priming than that of hydroprimed and unsoaked over the respective unstressed controls. The results thus suggest that the TU priming modulate expression of these genes thereby imparting cold tolerance in capsicum seedlings.
    Molecular Biology Reports 10/2012; · 2.51 Impact Factor
  • Vikas Yadav Patade, Sujata Bhargava, Penna Suprasanna
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    ABSTRACT: Seed priming is a well known pre-germination strategy that improves seed performance. However, biochemical and molecular mechanisms underlying priming mediated stress tolerance are little understood. Here, we report results of the study on growth, physiological characteristics and expression of stress responsive genes in salt primed sugarcane cv. Co 86032 plants in response to salt (NaCl, 150 mM) or iso-osmotic (-0.7 MPa) polyethylene glycol-PEG 8000 (20 % w/v) stress exposure for 15 days. Variable growth, osmolyte accumulation and antioxidant capacity was revealed among the primed and non-primed plants. The primed plants showed better tolerance to the salt or PEG stress, as revealed by better growth and lower membrane damage, through better antioxidant capacity as compared to the respective non-primed controls. Further, steady state transcript expression analysis revealed up regulation of sodium proton antiporter (NHX) while, down regulation of sucrose transporter (SUT1), delta ( 1 )-pyrolline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (PDH) in primed plants on exposure to the stress as compared to the non-primed plants. Transcript abundance of catalase (CAT2) decreased by about 25 % in leaves of non-primed stressed plants, however, the expression was maintained in leaves of the stressed primed plants to that of non-stressed controls. Thus, the results indicated priming mediated salt and PEG stress tolerance through altered gene expression leading to improved antioxidant capacity in sugarcane.
    Molecular Biology Reports 06/2012; 39(10):9563-72. · 2.51 Impact Factor
  • Vikas Yadav Patade, Sujata Bhargava, Penna Suprasanna
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    ABSTRACT: In order to discriminate between the ionic and osmotic components of salt stress, sugarcane (Saccharum officinarum L. cv. Co 86032) plants were treated with salt-NaCl or polyethylene glycol-PEG 8000 solutions (−0.7 MPa) for 15 days. Both the salt and PEG treatments significantly reduced leaf width, number of green leaves, and chlorophyll stability index. Osmotic adjustment (OA) indicated that both the stresses led to significant accumulation of osmolytes and sugars. Salt stressed plants appeared to use salt as an osmoticum while the PEG stressed plants showed an accumulation of sugars. Oxidative damage to membranes was not severe in plants subjected to salt or PEG stress. The salt stressed plants showed an increase in the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX), while PEG stress led to an increase in SOD but not APX activity as compared to the control. Thus, results indicate that the iso-osmotic salt or PEG stress led to differential responses in plants especially with respect to growth, OA, and antioxidant enzyme activities.
    Journal of Plant Interactions 12/2011; 6(4):275-282. · 0.90 Impact Factor
  • Vikas Yadav Patade, Archana Neeraj Rai, Penna Suprasanna
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    ABSTRACT: Identification of genes whose expression enables plants to adapt to any kind of stresses is integral to developing stress tolerance in crop plants. In this study, PCR-based cDNA suppression subtractive hybridization technique was used to construct sugarcane salt (NaCl) stress specific forward and reverse subtracted cDNA library. For this, mRNAs were pooled from the shoot and root tissues stressed with NaCl (200 mM) for various time intervals (0.5 to 18 h). Sequencing the clones from the forward subtracted cDNA library, we identified shaggy-like protein kinase (hereafter referred as sugarcane shaggy-like protein kinase, SuSK; NCBI GenBank EST database Acc: FG804674). The sequence analysis of the SuSK revealed homology to Arabidopsis thaliana shaggy-related protein kinase delta (E value, 1e(-108)), dzeta and iota. Alignment of the catalytic domain sequence of GSK-3/shaggy-like kinase with partial sequence of SuSK performed using ClustalW tool indicated kinase active-site signature sequence. Spatial and temporal transcript expression profiling of the SuSK gene based on Real-Time PCR revealed significant induction of transcript expression in response to short-term salt (NaCl 200 mM) or polyethylene glycol-8,000 (PEG; 20% w/v) induced osmotic stress in leaves and shoots of sugarcane plants. The transcript expression increased progressively under salt stress and reached to 1.5-fold of the control up to 8 h treatment. In response to PEG stress, the transcript expression increased by 1.5-fold over the control in 2-h treatment in leaf, whereas in shoots, the expression remained unchanged in response to the various treatments. Differences in growth parameters, relative water content, and membrane damage rate were statistically insignificant in the short-term salt or PEG-stressed plants as compared to the control, non-stressed plants. Expression analysis revealed the differential and temporal regulation of this gene under salt and PEG stress and that its early induction may indicate involvement in stress signaling.
    Protoplasma 07/2011; 248(3):613-21. · 2.86 Impact Factor
  • Vikas Yadav Patade, Sujata Bhargava, Penna Suprasanna
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    ABSTRACT: Semi-quantitative RT-PCR based transcript expression of stress responsive genes was studied in leaves of sugarcane plants exposed to short-term (up to 24 h) salt (NaCl, 200 mM) or polyethylene glycol-PEG 8000 (20% w/v) stress. Transient increase in expression of NHX (sodium proton antiporter), SUT1 (sucrose transporter1), PDH (proline dhydrogenase) and CAT2 (catalase2) was observed in response to 2-4 h PEG stress. However, salt stress imposed repression of NHX, PDH and CAT2 at these time points. The transcript level of the delta (1)-pyrolline-5-carboxylate synthetase (P5CS) increased slightly in salt treatment while in response to the PEG stress, the gene expression increased at 4 h treatment but then decreased considerably by 80% at 24 h. The results thus indicated differential regulation of these stress responsive genes in response to salt or PEG stress in sugarcane. Further, the transcript expression data was compared with that available for the Arabidopsis homologs at Arabidopsis eFP Browser and Genevestigator V3 tools. Understanding transcript gene expression patterns of the stress responsive genes may provide insights into complex regulatory network of stress tolerance.
    Molecular Biology Reports 06/2011; 39(3):3311-8. · 2.51 Impact Factor
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    ABSTRACT: Sugarcane (Saccharum officinarum L.) is one of the most important field crops grown in the tropics and sub-tropics. More than half of the world’s sugar is derived from sugar cane. Conventional methods have greatly contributed to crop improvement; however limitations such as complex genome, narrow genetic base, poor fertility, susceptibility to biotic and abiotic stresses and long duration to breed elite cultivars still impose a challenge. Sugarcane, thus, is a suitable candidate for application of biotechnology and genetic engineering tools. In this direction, in vitro culture systems and related biotechnologies have been developed as novel strategies for sugarcane improvement. Studies have been conducted towards employing in vitro culture combined with radiation/chemical induced mutagenesis for mutant isolation. Advancements in genomics tools have paved the way for a detailed understanding of the mechanism underlying biotic and abiotic stress responses. The potential of the current genomics programs, aimed at elucidating the structure, function, and interactions of the sugarcane genes, will revolutionize the application of biotechnology to crop improvement. Genetically modified sugarcane with increased resistance to agronomic traits including biotic and abiotic stresses, yield and juice could become useful in breeding for better varieties. This review outlines some of the biotechnological developments that are in place and tailored to address important issues related to sugarcane improvement. KeywordsSugarcane–Biotechnology–Genomics–Molecular markers–Stress tolerance–In vitro culture–Mutagenesis–Transgenic plants
    Sugar Tech 01/2011; 13(4):322-335. · 0.50 Impact Factor
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    ABSTRACT: The effect of optimal and supra-optimal concentrations (0, 200, 400 or 600mM) of NaCl on the growth, osmotic adjustment and The effect of optimal and supra-optimal concentrations (0, 200, 400 or 600mM) of NaCl on the growth, osmotic adjustment and antioxidant enzyme defence was studied in the in vitro cultures of Sesuvium portulacastrum. A significant increase in growth, tissue water content (TWC) and fresh to dry weight ratio (FW/DW) was observed in the shoots antioxidant enzyme defence was studied in the in vitro cultures of Sesuvium portulacastrum. A significant increase in growth, tissue water content (TWC) and fresh to dry weight ratio (FW/DW) was observed in the shoots exposed to 200mM salt. Minimum damage to the membrane in terms of low relative electrolytic leakage (REL) and malondialdehyde exposed to 200mM salt. Minimum damage to the membrane in terms of low relative electrolytic leakage (REL) and malondialdehyde (MDA) content and better osmotic adjustment at 200mM salt stress was coupled with the higher accumulation of sodium ions (MDA) content and better osmotic adjustment at 200mM salt stress was coupled with the higher accumulation of sodium ions and total soluble sugars as against low proline and glycine betaine contents. A fine tuning of antioxidant enzyme activities and total soluble sugars as against low proline and glycine betaine contents. A fine tuning of antioxidant enzyme activities (superoxide dismutase, catalase and ascorbate peroxidase) was also found to be responsible for the optimum growth of shoots. (superoxide dismutase, catalase and ascorbate peroxidase) was also found to be responsible for the optimum growth of shoots. In contrast, sub-optimal (0mM) and supra-optimal concentrations (400–600mM) of NaCl significantly affected the growth, water In contrast, sub-optimal (0mM) and supra-optimal concentrations (400–600mM) of NaCl significantly affected the growth, water status and increased the REL as well as MDA content of the shoots due to the accumulation of toxic concentrations of saline status and increased the REL as well as MDA content of the shoots due to the accumulation of toxic concentrations of saline ions. The highest accumulation of proline and glycine betaine in addition to antioxidant enzyme activities exhibited higher ions. The highest accumulation of proline and glycine betaine in addition to antioxidant enzyme activities exhibited higher osmotic adjustment and survival of the shoots under sub- or supra-optimal concentrations of NaCl as a penalty to reduced growth. osmotic adjustment and survival of the shoots under sub- or supra-optimal concentrations of NaCl as a penalty to reduced growth. KeywordsAntioxidant enzymes-In vitro growth responses-Osmolytes accumulation-Salinity- KeywordsAntioxidant enzymes-In vitro growth responses-Osmolytes accumulation-Salinity- Sesuvium portulacastrum Sesuvium portulacastrum -Shoot cultures -Shoot cultures
    Plant Cell Tissue and Organ Culture 01/2011; 104(1):41-49. · 2.61 Impact Factor
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    ABSTRACT: Cicer microphyllum, a wild relative of cultivated chickpea, is a high altitude cold desert-adapted species distributed in western and trans-Himalayas. A complementary DNA (cDNA) encoding metallothionein-like protein has been identified from a cold-induced subtraction cDNA library from C. microphyllum. The sequence of the cloned metallothionein gene from C. microphyllum (GQ900702) contains 240-bp-long open reading frame and encodes predicted 79-amino acid protein of 7.9 kDa. Sequence analysis identified the motifs characteristic of type II metallothionein and designated as CmMet-2. Southern hybridization confirms a single copy of the CmMet-2 gene in C. microphyllum genome. In situ hybridization indicated spatial transcript regulation of CmMet-2 in root and aerial parts and also confirmed through real-time PCR-based quantitative transcript analysis. The data revealed a significantly low level of transcript in the aerial parts than the roots. Quantitative analysis using real-time PCR assay revealed induction of transcript in all parts of plants in response to cold stress at 4°C. The transcript abundance was found to increase exponentially with time course from 6 to 24 h after exposure. Further, regulation of transcript accumulation in response to abscisic acid application, polyethylene glycol (100 μM)-induced osmotic stress, or ZnSO(4) (1 μM) foliar spray indicated by Northern hybridization suggests the involvement of CmMet-2 in multiple stress response.
    Protoplasma 12/2010; 248(4):839-47. · 2.86 Impact Factor
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    ABSTRACT: Sesuvium portulacastrum (L.) L., a facultative halophyte, is considered a suitable candidate for the phytoremediation of metals. An investigation of As accumulation and tolerance was conducted in Sesuvium plants upon exposure to As(V) (100-1000 μM) for 30 d. Plants demonstrated a good growth even after prolonged exposure (30 d) to high As(V) concentrations (1000 μM) and a significant As accumulation (155 μg g⁻¹ dry weight) with a bioaccumulation factor of more than ten at each concentration. The results of shoot and root dry weight, malondialdehyde accumulation, photosynthetic pigments, and total soluble proteins demonstrated that plants did not experience significant toxicity even at 1000 μM As(V) after 30 d. However, metabolites (total non-protein thiols and cysteine) and enzymes (serine acetyltransferase, cysteine synthase and γ-glutamylcysteine synthetase) of thiol metabolism, in general, remained either unaffected or showed slight decline. Hence, plants tolerated high As(V) concentrations without an involvement of thiol metabolism as a major component. Taken together, the results indicate that plants are potential As accumulator and may find application in the re-vegetation of As contaminated sites.
    Chemosphere 11/2010; 82(4):529-34. · 3.14 Impact Factor
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    ABSTRACT: The present study was aimed to evaluate the effect of different seed priming methods to enhance the sodium chloride (NaCl) and polyethylene glycol-8000 (PEG-8000) stress tolerance in Indian mustard (Brassica juncea L.). Seeds subjected to different priming treatments such as water (hydro-priming), calcium chloride (CaCl2) (chemo-priming), and abscisic acid (ABA) (hormonal-priming) showed increased rate of germination as compared to non-primed seeds. The primed and non-primed seeds were grown for 15days and then the seedlings were independently subjected to iso-osmotic salt (150mM NaCl) or PEG-8000 (20%) stress. The different biochemical responses were studied 10days after treatment. Under NaCl and PEG stress, the dry weight and total chlorophyll content were higher in primed sets as compared to non-primed treatment which was also evident by the phenotype of the seedlings. In general, the higher activities of superoxide dismutase and glutathione reductase resulted in lower oxidative damage, in terms of malondialdehyde content, under NaCl and PEG stress in hydro-primed set as compared to non-primed, ABA-, and CaCl2-primed treatments. Besides, the level of total phenolics and accumulation of osmolytes such as free proline, glycine betaine, and total soluble sugars was also lower in hydro-primed set as compared to other primed and non-primed treatments. The study thus suggests the use of hydro-priming as a simple and cost-effective strategy to alleviate the NaCl and PEG induced stress in B. juncea. Keywords Brassica juncea -Seed priming-Hydro-priming-Stress imprints-Antioxidant enzymes-Osmolytes accumulation
    Acta Physiologiae Plantarum 01/2010; 32(6):1135-1144. · 1.31 Impact Factor
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    ABSTRACT: Transposable elements constitute a large fraction of plant genomes, and changes due to TE mobilization have provided powerful genetic and molecular tools. Somaclonal variation is a common phenomena caused either by various extrinsic and intrinsic factors related to in vitro culture or transposon activity has been detected under cell culture or tissue culture milieu. In this study, variability among direct somatic embryogenesis (DSEM) and indirect somatic embryogenesis (ISEM) regenerants of sugarcane cv. CoC-671 was studied using Ac transposon insertion polymorphism. A total of 254 amplification products were obtained ranging from 0.5 Kb to 2 Kb. The DNA profile revealed genetic polymorphism among the ISEM regenerants compared to DSEM regenerants. There was n uniform insertion pattern of the Ac like transposons among the DSEM regenerants, whereas it was variable in case of ISEM regenerants. The results on the insertion polymorphism of Ac homologous regions among the ISEM regenerants indicate that the transposition occurred during in vitro culture and that this marker system could be useful in profiling of genetic variation. KeywordsSugarcane-somatic embryogenesis- Ac like transposable elements-polymorphism
    Sugar Tech 01/2010; 12(1):26-30. · 0.50 Impact Factor
  • Vikas Yadav Patade, Sujata Bhargava, Penna Suprasanna
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    ABSTRACT: Sugarcane, a glycophyte grown in the tropical and subtropical regions, is frequently subjected to soil salinity, affecting the yield and quality of the harvest. The ameliorative efficiency of salt priming on emergence and plantlet growth was examined in sugarcane cultivars which are known to vary in salt tolerance under field conditions. Salt priming with NaCl (100 mM) improved both the percent and rate of germination of the sets of the tolerant (Co 62175) and moderately tolerant (CoM 265) varieties compared to sensitive (CoC 671) and test variety (Co 86032). Salt priming during germination also improved the growth performance of two-month-old sugarcane plants in terms of shoot length, shoot and root fresh weight when subjected to 15 day iso-osmotic (−0.7 MPa) NaCl (150 mM) or polyethylene glycol (PEG 8000; 20%, w/v) stress. The primed plants exhibited lower salt- and dehydration-induced leaf senescence. The results suggest salt priming as an efficient approach for imparting abiotic stress tolerance in sugarcane.
    Agriculture, Ecosystems & Environment. 01/2009;

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144 Citations
39.56 Total Impact Points

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Institutions

  • 2008–2012
    • Bhabha Atomic Research Centre
      • Nuclear Agriculture & Bio Technology Division
      Mumbai, State of Maharashtra, India
  • 2009–2011
    • University of Pune
      • Department of Botany
      Poona, Mahārāshtra, India
  • 2006
    • Marathwada Agricultural University
      Parbaini, Mahārāshtra, India