Mohamadhasan Bozorgmehrifard

University of Tehran, Teheran, Tehrān, Iran

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Publications (2)1.09 Total impact

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    ABSTRACT: A total of 512 tissue samples collected from 30 farms located in various states of Iran during 2008-2009 as part of a program to monitor avian influenza viruses (AIVs) infection in Iran's poultry population. To determine the genetic relationship of Iranian viruses, neuraminidase (NA) genes from ten isolates of H9N2 viruses isolated from commercial chickens in Iran during 2008-2009 were amplified and sequenced. The viruses' neuraminidase gene was >90% similar to those of A/Quail/Hong Kong/G1/97 (H9N2) sublineage. The neuraminidase stalk regions in these Viruses had no deletion as compared to that of chicken/Beijing/1/94 sublineage (Beijing-like viruses) and the two human isolates A/HK/1073/99, A/HK/1074/99. Phylogenetic analysis of neuraminidase (NA) gene showed that it shares a common ancestor A/Quail/Hong Kong/G1/97 isolate which had contributed the internal genes of the H5N1 virus. The results of this study indicated that No (Beijing-like) virus and (Korean-like) virus were found in chickens in Iran, and the NA genes of H9N2 influenza viruses circulating in Iran during the past years were well conserved and the earlier Iranian isolates may be considered to represent such a progenitor.
    Tropical Animal Health and Production 07/2011; 44(3):419-25. · 1.09 Impact Factor
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    ABSTRACT: A total of 512 tissue samples collected from 30 farms located in various states of Iran during 2008-2009 as part of a program to monitor AIV infection in Iran poultry population. Avian influenza virus was isolated from poultry farms with history of respiratory illness and increased mortality. Nucleotide sequence analysis of five representative isolates confirmed that all isolates possessed one type of amino acid motif (R-S-S-R/GL) at cleavage site of HA, Deduced amino acid sequences showed the presence of L226 (234 in H9 numbering) in all five Iranian isolates which indicates a preference to binding of α (2-6) sialic acid receptors. The viruses' surface glycoproteins genes were >90% similar to those of A/Quail/Hong Kong/G1/97 (H9N2) lineage. The neuraminidase stalks regions in these viruses had no deletion as compared to that A/Dk/HK/ Y280/97 lineage (Ck/Bei-like viruses) and the 2 human isolates A/HK/1073/99, A/HK/1074/99. The hemadsorbing site of neuraminidase had up to 3 amino acid substitutions and is different from those of earlier Iranian viruses. Phylogenetic analysis HA and NA genes showed that they share a common ancestor Qa/HK/G1/97 isolate which had contributed internal genes of H5N1 virus.
    Journal of Biological Sciences. 01/2010;