Shiwen Xu

Northeast Agricultural University, Charbin, Heilongjiang Sheng, China

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Publications (31)51.99 Total impact

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    ABSTRACT: Selenium (Se) deficiency induces pancreatic atrophy in chickens, but the molecular mechanism remains unclear. In this study, we investigated the effect of dietary Se deficiency on the expressions of 25 selenoproteins and the content of nitric oxide (NO) and examined the relationship between selenoproteins and NO. Chickens (180; 1 day old) were randomly divided into two groups, low (L) group (fed with Se deficient (Se 0.033 mg/kg) diet) and control (C) group (fed with normal (Se 0.2 mg/kg) diet). Then, pancreas was collected at 15, 25, 35, 45, and 55 days, and the content of NO, the activity of inducible NO synthase (iNOS), and the messenger RNA (mRNA) levels of 25 selenoproteins and iNOS were measured. The results showed that 25 selenoproteins were decreased (P < 0.05) by Se deficiency. Among them, thioredoxin reductase 1 (TXNRD1), selenoprotein S (SELS), selenoprotein U (SELU), selenoprotein X1 (SEPX1), and selenoprotein synthetase 2 (SPS2) were highly and extensively expressed than other types of selenoproteins in pancreas of chickens (P < 0.05). Thioredoxin reductase 2 (TXNRD2), glutathione peroxidase 1 (GPX1), glutathione peroxidase 3 (GPX3), selenoprotein I (SELI), iodothyronine deiodinase 1 (DIO1), selenoprotein P1 (SEPP1), selenoprotein W1 (SEPW1), selenoprotein O (SELO), selenoprotein T (SELT), selenoprotein M (SELM), selenoprotein X1 (SEPX1), and SPS2 were excessively decreased (P < 0.05). Meanwhile, NO content, iNOS activity, and mRNA level were increased strikingly compared with C group (P < 0.05). The correlation analysis suggested that NO had a strong negative correlation with GPX1, glutathione peroxidase 2 (GPX2), GPX3, DIO1, selenoprotein K (SELK), SELI, SEPX1, and SPS2. These results suggested that Se deficiency induced pancreatic injury by influencing NO and selenoproteins in pancreas of chickens. Thus, it offers some information on the mechanism of pancreatic injury induced by Se deficiency.
    Biological trace element research. 10/2014;
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    ABSTRACT: The aim of the present study was to investigate the effects of selenium (Se) deficiency on the expressions of heat shock proteins (Hsp90, 70, 60, 40, and 27) and nitric oxide (NO) levels in neutrophils of broilers. One hundred eighty 1-day-old broilers were randomly assigned into two groups and were fed on a low-Se diet (0.008 mg/kg Se) or a control diet (0.2 mg/kg Se), respectively. Then, the messenger RNA (mRNA) levels of Hsp90, 70, 60, 40, and 27, induced nitric oxide synthase (iNOS), and NO levels were examined. The results showed that Se deficiency increased the mRNA levels of Hsps and iNOS and induced higher level of NO in chicken neutrophils (P < 0.05). It showed that the expression of Hsp40 increased higher than other Hsps in neutrophils, which indicated that it might play the crucial protective role in neutrophils. In addition, correlation analysis showed that iNOS had the biggest correlation with Hsp60, which indicated that Hsp60 might play an important function in inhibiting the production of NO, and the correlation coefficient between Hsp60 and Hsp70 was over 0.9, which indicated that they might have a synergistic effect. These results suggested that the level of NO and Hsp expression levels in neutrophils can be influenced by Se deficiency. And Hsp40 might play the crucial protective role in neutrophils induced by Se deficiency.
    Biological trace element research. 10/2014;
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    ABSTRACT: Cadmium could induce the damage of endoplasmic reticulum. In the present study, we investigated the effect of Cadmium on messenger RNA expressions of endoplasmic reticulum resident selenoproteins, selenoprotein K, selenoprotein N, selenoprotein S, and selenoprotein T, in cultured chicken lymphocytes and the antagonistic effect of Selenium. Chicken splenic lymphocytes were treated with 10(-7) mol/L Selenium, 10(-6) mol/L Cadmium, and the mixture of 10(-6) mol/L Selenium and 10(-7) mol/L Cadmium in the culture medium for 12, 24, 36, and 48 h, respectively. Then, we detected the messenger RNA expressions of selenoprotein K, selenoprotein N, selenoprotein S, and selenoprotein T by using real-time polymerase chain reaction method. The results indicated that Selenium significantly increased the expressions of selenoprotein K, selenoprotein N, selenoprotein S, and selenoprotein T, which were reduced by Cadmium in chicken splenic lymphocytes. It indicated that endoplasmic reticulum was one target of Cadmium toxication, and Cadmium toxicity might be related to the reduced expressions of selenoprotein K, selenoprotein N, selenoprotein S, and selenoprotein T in chicken lymphocytes. Selenium reserved the protective role by increasing the expressions of selenoprotein K, selenoprotein N, selenoprotein S, and selenoprotein T. The present study provided a useful clue to investigate the possible pathogenesis of Cadmium toxicity.
    Biological trace element research. 10/2014;
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    ABSTRACT: Dietary selenium (Se) deficiency induces muscular dystrophy in chicken, but the molecular mechanism remains unclear. The aim of the present study was to investigate the effect of dietary Se deficiency on the expressions of 25 selenoproteins. One-day-old broiler chickens were fed either an Se deficiency diet (0.033 mg Se/kg; produced in the Se-deficient area of Heilongjiang, China) or a diet supplemented with Se (as sodium selenite) at 0.2 mg/kg for 55 days. Then, the mRNA levels of 25 selenoproteins in chicken muscles were examined, and the principal component was further analyzed. The results showed that antioxidative selenoproteins especially Gpxs and Sepw1 were highly and extensively expressed than other types of selenoproteins in chicken muscles. In 25 selenoproteins, Gpxs, Txnrd2, Txnrd 3, Dio1, Dio 3, Selk, Sels, Sepw1, Selh, Sep15, Selu, Selpb, Sepp1, Selo, Sepx1, and SPS2 were downregulated (P < 0.05), and other selenoproteins were not influenced (P > 0.05). Se deficiency decreased the expressions of 19 selenoproteins (P < 0.05), 11 of which were antioxidative selenoproteins. And, principal component analysis (PCA) further indicated that antioxidative selenoproteins, especially Gpx3, Gpx4, and Sepw1, may play crucial roles in chicken muscles. However, compared with these antioxidative selenoproteins, some other lower expressed selenoproteins (Dio1, Selu, Selpb, Sepp1) were excessively decreased (more than 60 %, P < 0.05) by Se deficiency. Thus, it may save the limited Se levels and be beneficial to remain the level of some crucial selenoproteins. These results suggested that Se deficiency mainly influenced the expressions of antioxidative selenoproteins in chicken muscles. And, antioxidative selenoproteins especially Gpxs and Sepw1 may play a crucial role in chicken muscles. Thus, it helps us focus on some specific selenoproteins when studying the role of Se in chicken muscles.
    Biological trace element research. 10/2014;
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    ABSTRACT: Atrazine (ATR) and chlorpyrifos (CPF), widely used in agriculture, have resulted in a series of toxicological and environmental problems. We investigated the activities of the biotransformation enzymes ethoxyresorufin-O-deethylase (EROD) and pentoxyresorufin-O-deethylase (PROD), total cytochrome P450 (CYP), CYP1A mRNA level and level of tissue ATR, CPF, and their metabolites in the liver of common carp (Cyprinus carpio L.) after a 40-d exposure to CPF and ATR, alone or in combination, and a 20-d recovery. In the present study, juvenile common carp was exposed to ATR (at concentrations of 4.28, 42.8 and 428μgL(-1)), CPF (1.16, 11.6 and 116μgL(-1)), and ATR/CPF mixture (at concentrations of 1.13, 11.3 and 113μgL(-1)). A general increasing trend for the activity of the biotransformation enzymes (EROD and PROD), CYP and CYP1A mRNA level was observed in the liver of common carp exposed to ATR, CPF and the ATR/CPF mixture. In addition, ATR, CPF, and their metabolites demonstrated a high accumulation in the liver. These results demonstrated that the CYP system in fish could be used as a biomarkers in evaluating the impact of ATR and CPF exposure on the common carp.
    Chemosphere 02/2014; · 3.14 Impact Factor
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    ABSTRACT: Atrazine (ATR) and chlorpyrifos (CPF) are toxic and subject to long-term in vivo accumulation in different aquatic species throughout the world. The purpose of the present study was to examine the effect of ATR, CPF and combined ATR/CPF exposure on cytokines in the head kidney and spleen of common carp (Cyprinus carpio L.). The carp were sampled after a 40-d exposure to CPF and ATR, individually or in combination, followed by a 40-d recovery to measure the mRNA expression of IL-6fam (IL-6), IL-8, TNF-α, IL-10 and TGF-β1 (TGF-β) in the head kidney and spleen tissues. These results showed that the expression of cytokines IL-6, IL-8 and TNF-α in the head kidney and spleen was upregulated following ATR, CPF and mixed ATR/CPF exposure compared with the control group. The expression of IL-10 and TGF-β mRNA was significantly inhibited in both head kidney and spleen of carp exposed to ATR, CPF and the ATR/CPF mixture. The results suggested that long-term exposure of ATR, CPF and the ATR/CPF mixture in aquatic environments can induce the dysregulation of pro-/anti-inflammatory cytokine expression. The information regarding the effects of ATR and CPF on cytokine mRNA expression generated in this study will be important information for pesticides toxicology evaluation.
    Pesticide Biochemistry and Physiology 01/2014; · 2.11 Impact Factor
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    ABSTRACT: Pesticide exposure has repeatedly been associated with cancers, although the molecular mechanisms behind this association are largely undetermined. Abnormal DNA methylation plays a key role in the process of some disease. However, little was known about the effect of pesticides on DNA methylation in the common carp. In this study, we investigated the mRNA levels of DNA methyltransferases (DNMTs) and methyl-CpG-binding protein DNA-binding domain protein 2 (MBD2) as well as the DNA methylation levels in the liver, kidney and gill of the common carp (Cyprinus carpio L.) after 40-d exposure to atrazine (ATR) and chlorpyrifos (CPF) alone or in combination, and a 40-d recovery period. Juvenile common carp were exposed to various concentrations of ATR (at concentrations of 4.28, 42.8 and 428 μg/L), CPF (1.16, 11.6 and 116 μg/L), and an ATR/CPF mixture (at concentrations of 1.13, 11.3 and 113 μg/L). The results revealed that the levels of genomic DNA methylation decreased in all tissues after 40 d of exposure to ATR and CPF either individually or in combination. Moreover, the mRNA expression of DNMTs was down-regulated in all treatment groups. In contrast, the mRNA expression of MBD2 was up-regulated. These results demonstrated that long-term exposure to ATR, CPF and ATR/CPF mixtures could disrupt genomic DNA. It might imply that DNA methylation is involved in the toxicity caused by ATR and CPF in the common carp.
    Ecotoxicology and Environmental Safety 01/2014; 108:142–151. · 2.20 Impact Factor
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    ABSTRACT: To determine the effect of Avermectin (AVM) on inflammation damage in king pigeon brain, eighty two-month-old American king pigeons were randomly divided into four groups, and were fed with either commercial diet or AVM-supplemented diet containing 20mgkg(-1)diet, 40mgkg(-1)diet, and 60mgkg(-1)diet AVM for 30, 60 and 90d, respectively. Then, the expression level of inflammatory factors (iNOS, PTGEs, NF-κB), histological damage, and ultra-structural damage were examined. It showed that AVM caused higher expressions (P<0.05) of iNOS, PTGEs, NF-κB with disorganized histological and ultra-structural structures in cerebrum, cerebellum, and optic lobe. Meanwhile, inflammatory and histopathological damage were induced by AVM in king pigeon brains. In addition, the main targeted organelle in nervous system was mitochondria, which indicated that mitochondria may be relevant to the process of inflammation induced by AVM. To our best knowledge, this is the first report to study the toxic effect of AVM on inflammatory damage in king pigeon. Thus, the information presented in this study is believed to be helpful in supplementing data for further AVM toxicity study.
    Chemosphere 10/2013; · 3.14 Impact Factor
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    ABSTRACT: This study assessed the impacts of atrazine (ATR), chlorpyrifos (CPF) and combined ATR/CPF exposure on the immune organs of common carp (Cyprinus carpio L.). The carp were sampled after a 40-d exposure to CPF and ATR, individually or in combination, followed by a 20-d recovery to measurethe acid phosphatase (ACP), alkaline phosphatase (ALP), Na(+)/K(+)-ATPase, superoxide dismutase (SOD) and malondialdehyde (MDA) content. In addition, we also investigated histopathological changes and pesticide residues in the spleen and head kidney. The results revealed that the responses of SOD, MDA, ALP, ACP, and Na(+)/K(+)-ATPase to ATR, CPF and ATR/CPF combination exposure presented in a dose-dependent manner. The ACP activity and MDA content were significantly induced with increasing concentrations of ATR, CPF and combined ATR/CPF. However, the enzyme activities (SOD, ALP, and Na(+)/K(+)-ATPase) were reduced under exposure to increasing concentrations of these compounds. The accumulated amounts of ATR, CPF, and their metabolites in the spleens and head kidneys tissues exhibited dose-dependency. Pathological changes included tissue damage that was more severe with increasing exposure doses. SOD activity negatively correlated with MDA accumulation. The biochemical parameters exhibited sensitivity to ATR and CPF, suggesting that they may act as potential biomarkers for assessing the environmental ATR and CPF risk for carp.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 08/2013; · 2.99 Impact Factor
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    ABSTRACT: Nitrobenzene (NB) has become an important pollutant in the environment, but its potential effects on non-target species such as drake remain unknown. In this study, we investigated the oxidative stress response, the CYP450 system and histopathological changes of the liver of NB-treated drakes for 40d. Our results indicated that NB induced significant changes in antioxidant enzyme (SOD, CAT and GSH-Px) activities and the MDA content in the liver of the drakes. NB caused a condition-dependent increase in APND, EROD and ECOD isoenzyme activities and CYP450 content with increased exposure dose. Significant histological alternations were observed in the liver of NB-treated drakes and the pathological changes revealed tissue damage that was more severe with increasing of exposure dose. To our knowledge, this is the first study to report the chronic effects of NB on oxidative stress, the CYP450 system and histopathology in the drakes. These significant effects caused by NB reveal that these indices can be used as biomarker for monitoring NB as an environmental pollutant. Thus, future studies are needed to fully understand the exact mechanisms of these findings.
    Research in Veterinary Science 08/2013; · 1.77 Impact Factor
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    ABSTRACT: Atrazine (ATR) and chlorpyrifos (CPF) are the most common pesticides found in freshwater ecosystems throughout the world. In the present study, the common carps were exposed to ATR, CPF and their mixture as describe in Table 1. Then we investigated the HSPs (HSP60, HSP70 and HSP90) expression in the brain of common carp by western blot and real-time PCR. Results indicated that HSP60 and HSP70 were significantly increased as compared to corresponding controls after 40 d exposure (P < 0.05). While the increased expression levels of HSP90 in exposure groups was only observed at 4.28 μg/L ATR group and 1.16 μg/L CPF group, and after 40 d recovery, the expression levels of HSP90 were higher than most of exposure groups (P < 0.05). It was suggested that the increased gene expression of HSPs was possible to protect injured cell mass caused by ATR, CPF and their mixture exposure. The present results provided new insights into the mechanisms used by fish to adapt to stressful environments.
    Pesticide Biochemistry and Physiology 01/2013; 107(2):277–283. · 2.11 Impact Factor
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    ABSTRACT: Insecticides and herbicides are widely used in modern agricultural production. The intensive use of insecticide chlorpyrifos (CPF) and herbicide atrazine (ATR) has resulted in serious environmental problems. Herein, we investigated alteration in activity and mRNA levels of AChE in the liver, kidney and gill from common carp after 40d exposure to CPF and ATR alone or in combination and 20d recovery treatment. Results indicated that activity and mRNA levels of AChE at all high-dose groups have been significantly decreased after CPF and ATR alone or ATR/CPF mixture exposure, and the changes were improved in the end of recovery tests in varying degrees, the activity and gene expression of AChE in the joint toxicity of ATR and CPF groups were significantly lower than that in the single toxicant group. Our study suggests that the decrease of AChE activity observed at all high-dose groups (CPF and ATR alone or in combination) may be directly related to a lower AChE expression, and the joint toxicity of ATR and CPF is higher than ATR and CPF alone.
    Environmental toxicology and pharmacology. 11/2012; 35(1):47-54.
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    ABSTRACT: Although the chaperone role of heat shock proteins (HSPs) has been demonstrated in invertebrates, the function of HSPs in vertebrates, especially in fish, remains unclear. In this study, relative changes in the mRNA abundance of the HSP70 gene were examined by real-time PCR in the muscle, spleen, head kidney, heart, liver, brain, kidney and gill of common carp. Results indicated that the highest and lowest levels of HSP70 expression were found in the heart and muscle, respectively, and the highest and lowest levels of HSC70 expression were found in the spleen and muscle, respectively. In addition, we investigated differential HSP70 gene expression in common carp after a 40-d exposure to chlorpyrifos (CPF) and atrazine (ATR), alone or in combination, and after a 20-d recovery. Results indicated that the expression of carp HSP70 and 70-kDa heat shock cognate protein (HSC70) with ATR and CPF treatment alone or in combination was significantly upregulated. The present results provide new insights into the mechanisms used by fish to adapt to stressful environments.
    Chemosphere 07/2012; · 3.14 Impact Factor
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    ABSTRACT: Atrazine (ATR) and chlorpyrifos (CPF) are the most common pesticides found in freshwater ecosystems throughout the world. Herein, we investigated the oxidative stress responses and histopathological changes in the liver and gill of common carp after a 40-d exposure to CPF and ATR, alone or in combination, and a 20-d recovery treatment. We found that exposure to ATR, CPF or their mixture for 40 d could induce decrease in antioxidant enzyme (SOD, CAT and GSH-Px) activities and increase in MDA content in a dose-dependent manner in the liver and gill of common carp. Especially with regard to the pathological changes, the tissue damage increased in severity in a dose-dependent manner. The liver tissue of common carp revealed different degree of hydropic degeneration, vacuolisation, pyknotic nuclei, and fatty infiltration. The gills of common carp displayed varied degrees of epithelial hypertrophy, telangiectasis, oedema with epithelial separation from basement membranes, general necrosis, and epithelial desquamation. After a 20-d recovery treatment, the antioxidant enzyme activities and MDA content were significantly lower (p < 0.05) than in the corresponding exposure groups in all of the highest doses, but not in the lower doses. To our knowledge, this is the first report of subchronic oxidative stress and histopathological effects caused by ATR, CPF and their mixture in the common carp. Thus, the information presented in this study is helpful to understand the mechanism of ATR-, CPF- and ATR/CPF-mixture-induced oxidative stress in fish.
    Pesticide Biochemistry and Physiology 05/2012; 103(1):74–80. · 2.11 Impact Factor
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    ABSTRACT: We investigated oxidative stress response and histopathological changes in the brain and kidney of the common carp after a 40-d exposure to CPF and ATR, alone or in combination, and a 20-d recovery. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) content were measured using standard assays. Our results indicated that exposure to ATR, CPF or a combination of the two for 40 d induced significant changes in antioxidant enzyme (SOD, CAT and GSH-Px) activities and MDA content in the brain and kidney of the common carp. Pathological changes included tissue damage that was more severe with increased of exposure dose. To our knowledge, this is the first report to study oxidative stress and histopathological effects caused by subchronic exposure to ATR, CPF and ATR/CPF combination on common carp. The information presented in this study may be helpful to understanding the mechanisms of ATR-, CPF- and ATR/CPF combination-induced oxidative stress in fish.
    Chemosphere 03/2012; 88(4):377-83. · 3.14 Impact Factor
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    ABSTRACT: Manganese (Mn) is an essential trace element required for normal development and bodily function. However, little is known about the effect of excessive amounts of Mn in immune organs of poultry. The aim of this study was to investigate the effect of dietary Mn on the content of trace elements, such as copper (Cu), iron (Fe), zinc (Zn), calcium (Ca), and selenium (Se), and the mRNA level of IL-1β and IL-2 in immune organs (spleen, thymus, and bursa of Fabricius) and the content of IL-1β and IL-2 in serum of poultry. Fifty-day-old male Hyline cocks were fed either a commercial diet or a Mn-supplemented diet containing 600, 900, and 1,800 mg/kg. The immune organs were collected at 30, 60, and 90 days, respectively, and the content of trace elements and the mRNA level of IL-1β and IL-2 were examined; the serum were collected and the IL-1β and IL-2 contents detected. The results showed that Mn content in immune organs increased and Fe, Zn, and Ca contents decreased; however, Cu and Se contents showed no difference. IL-1β and IL-2 mRNA levels and IL-1β and IL-2 contents decreased. The present study demonstrates that excess exposure to Mn results in metal accumulations in immune organs. Manganism can disturb the balance of trace elements in immune organs and induce immune suppression in the molecular level; therefore, the immune function of cocks are also suppressed after manganism.
    Biological trace element research 03/2012; 148(3):336-44. · 1.92 Impact Factor
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    ABSTRACT: Glutathione S-transferase isoenzymes (GSTs) play a critical role in detoxification pathways. Here we report the tissue distribution of four antioxidant GSTs gene in common carp, and their expression profiles. We also investigated the GSTs activity in different tissues after exposure to the agricultural chemicals atrazine (ATR), chlorpyrifos (CPF), and their mixture. Relative changes in the mRNA abundance of the GST isoforms were examined by real time PCR in liver, brain, kidney and gill of common carp. After exposure and recovery, we observed a statistically significant decrease in the GSTs activity in animals exposed to high concentrations of ATR (428 μg/L), CPF (116 μg/L), and their mixture (113 μg/L). At basal levels of tissue expression, four GSTs transcript were detected in liver, brain, kidney, and gill. High expression levels were found in all examined tissues. Transcription of some GST isoforms, GST kappa (GSTK), GST theta (GSTT) and GST rho (GSTR), decreased after exposure to CPF and ATR for the entire experimental period in both the kidney and gill. However, increased transcription of GST mu (GSTM) was observed in the kidney or gill 20 d after exposure to ATR or CPF, respectively. Transcription of both GSTT and GSTR was inhibited for the entire experimental period in the brain, kidney and gill of animals exposed to the ATR/CPF mixture, but transcription of GSTM was induced in the liver after 40 d of exposure. In summary, changes in the GSTs activity and their transcription varied within each organ and among organs of common carp after exposure to ATR, CPF, and their mixture.
    Environmental toxicology and pharmacology. 03/2012; 33(2):233-44.
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    ABSTRACT: To investigate the toxicity of cadmium (Cd) on female reproduction in birds, this study was conducted to determine the changes in biochemical parameters of serum and ovary tissue caused by dietary cadmium in hens. Ninety 50-day-old hyline white hens were randomly divided into three groups (30 hens per group): a control group was fed with basal diet, a low dose group was fed with basal diet containing 140 mg/kg CdCl(2) and a high dose group was fed with basal diet containing 210 mg/kg CdCl2. After being treated with Cd for 20, 40 and 60 days, ovary and serum samples were collected and examined for Cd content, histological evaluations, malondialdehyde (MDA) content, glutathione peroxidase (GPx) content, activities of superoxide dismutase (SOD), nitric oxide (NO) content, nitric oxide synthase (NOS) activity, and serum estradiol and progestogen levels. The results showed that the content of Cd, MDA, NO and the activity of NOS in ovary and serum were increased (P < 0.05), while the level of GPx and the activity of SOD were decreased (P < 0.05) in low dose and high dose groups. A time- and dose-dependent correlation was observed between serum and ovary tissue cadmium levels. The number of apoptotic cells in the ovary was increased in the Cd treatment group (P < 0.05). Extensive damage was observed in the ovary. The level of estradiol and progestogen in the serum of low dose and high dose groups was decreased significantly (P < 0.05). It indicated that Cd exposure resulted in oxidative damage of hens' ovary tissue by altering antioxidant defense enzyme systems, lipid peroxidation, apoptosis and endocrine disturbance which may be possible underlying reproductive toxicity mechanisms induced by Cd.
    Biological trace element research 02/2012; 148(1):53-60. · 1.92 Impact Factor
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    ABSTRACT: To investigate the species and distribution of bacteria in a habitat that was polluted by a fermentative pharmaceutical sewage. The bacteria were isolated from waste water, riverbed soil and sludge by nutrient agar and nutrient broth, which were collected from the sewage outfall of a fermentative pharmaceutical factory. The bacteria were identified by Gram staining and biochemical tests, while the bacterial community diversity had been analyzed. The results showed that logarithm of the total number of viable cells (TVC) in effluent samples was 5.348,which was higher than 5.136 in riverbed soil and 5.246 in sludge. 19 genera and 22 species had been isolated from water samples and riverbed soil samples including 14 genera and 16 species, 8genera and 10species. Shannon-Wiener (H) and Simpson (D) in water sample is 2.902 and 0.9422, which was higher than 2.0253 and 0.8600 in riverbed and 2.5993 and 0.9216 in sludge. Pielou (J) was 0.9856, 0.9849 and 0.9740 in water samples, riverbed samples and sludge samples respectively. Total number of bacteria and diversity in water samples was higher than riverbed and sludge.
    Remote Sensing, Environment and Transportation Engineering (RSETE), 2012 2nd International Conference on; 01/2012
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    ABSTRACT: The Sel-W gene encoding avian selenoprotein W was cloned into the vector pGEX-6p-1 with GST tag. The recombinant protein Sel-W was expressed in Escherichia coli BL21 (DE3) after induction with 1 mM IPTG. Female 10-week-old BALB/c mice were immunized with the purified Sel-W recombinant protein emulsified in Freund's adjuvant. Two monoclonal antibodies against the Sel-W, 1B8 and 4H5, were produced by lymphocyte hybridoma technique. Subtypes of the MAbs 1B8 and 4H5 were all IgM, and their ascitic fluid titers were 1:2800 and 6400, respectively. In specificity, the MAbs 1B8 and 4H5 showed positive reaction with the recombinant Sel-W protein with GST tag and the synthesized Sel-W protein, and could not react with the GST tag and the recombinant Sel-N protein. In sensitivity, the detection limits of the MAbs 1B8 and 4H5 for the recombinant Sel-W protein and the synthesized Sel-W protein were 39 ng/mL and 52 ng/mL, respectively. These data suggest that the MAb 1B8 and 4H5 will have a potential use for detection and function analysis of the avian Sel-W.
    Hybridoma (2005) 12/2011; 30(6):563-6. · 0.33 Impact Factor