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ABSTRACT: Isolated segmentectomy VIII is a technically demanding operative procedure and is reported only rarely. To our knowledge, no reports on anatomic segmentectomy based on an intrahepatic approach have been described. For cirrhotic patients with hepatocellular carcinoma (HCC) limited to segment VIII, this is a parenchyma-preserving hepatectomy that can be tolerated.
Eighteen patients with HCC underwent anatomic segment VIII segmentectomy from January 2005 to January 2008 in our institution. The operative techniques, postoperative, and oncologic outcomes were reviewed.
Anatomic segmentectomy VIII was feasible with the technology described herein in all patients. The perioperative and oncologic outcomes were comparable with those of other similar hepatic resections. The median follow-up time was 28 months. The 3-year survival rate was 65%.
Although complex and technically demanding, an intrahepatic Glissonian approach for anatomic segmentectomy of segment VIII is an oncologically radical but parenchyma-sparing hepatic resection. In terms of preserving greater functioning liver parenchyma, it may be a safe and effective alternative to extensive hepatectomy.
Surgery 08/2009; 146(5):854-60. · 3.10 Impact Factor
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ABSTRACT: Matrix metalloproteinase-1 (MMP-1) 1G/2G (-1,607) polymorphisms have been identified and shown to influence the transcription of the MMP-1 gene. In order to compare the expression of MMP-1 with different MMP-1 gene promoter alleles after force loading, human periodontal ligament (PDL) cells were cultured and genotyped into three alleles by polymerase chain reaction and restriction endonuclease cleavage. The three genotypes of PDL cells were centrifuged and the expression of MMP-1 mRNA and protein were determined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. The results showed that centrifugal force upregulated the expression of both MMP-1 mRNA and protein in all three genotypes of PDL cells. The induction of MMP-1 by force was significantly greater in cells with a 2G/2G genotype or a 1G/2G genotype than in cells homozygous for the 1G allele. The MMP-1 mRNA and protein levels were significantly higher for cells with the 2G allele than for cells with the 1G/2G allele or the 1G allele. These results suggest that a single nucleotide polymorphism in the -1,607 bp MMP-1 promoter region might be associated with the difference observed in the endogenous expression of MMP-1 in PDL cells under mechanical force.
European Journal Of Oral Sciences 08/2008; 116(4):319-23. · 1.88 Impact Factor
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Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 05/2006; 31(2):299-300.
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ABSTRACT: Timolol-loaded poly(D,L-lactide-co-glycolide; PLGA) films were prepared for achieving the long-term intraocular pressure (IOP) lowering effect on glaucoma treatment. The physicochemical properties and in vivo effects of films were determined and characterize the delivery system. PLGA, span 20, propylene glycol (PG), and timolol base were dissolved in dichloromethane (DCM) and kept in a stainless mold; timolol films were prepared after the evaporation of DCM. Timolol disc-shape film preparation (TDF), containing 1 mg of timolol in 0.37 cm(2) area, was fabricated by using a trephine and placed onto the cul de sac of alpha-chymotrypsin-induced ocular hypertension rabbits for assessing the IOP lowering effect. The prepared films characterized a Young's modulus ranged from 1.13 to approximately 2.49 MPa, and related to the content percentages of PLGA, PG, and the residual DCM. The timolol film could maintain drug release for 1 week. Following a single-dose application in ocular hypertension rabbits, the prepared TDF could achieve a long-term IOP lowering effect and maintain the IOP change (in comparison with baseline) of approximately 7 mmHg within 1 week. The aqueous humor levels of timolol were low within a range of 0.8 to approximately 0.24 microg/mL for the initial 24 h and less than 0.15 microg/mL for 4-7 days. The investigated film formulation might be potentially developed for the application of long-term ocular delivery systems.
Journal of Ocular Pharmacology and Therapeutics 01/2006; 21(6):445-53. · 1.51 Impact Factor
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Yu Wen,
Yong-guo Li,
Zhu-lin Yang,
Xue-jun Wang,
Hong Wei,
Wei Liu,
Xiong-ying Miao,
Qun-wei Wang, Sheng-fu Huang,
Jing Yang,
E Olavi Kajander,
Neva Ciftcioglu
Chinese medical journal 04/2005; 118(5):421-4. · 0.86 Impact Factor
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ABSTRACT: Waldeyer's ring is the most vulnerable site outside lymph nodes in non-Hodgkin's lymphoma (NHL). Its imaging features in NHL of early stage were similar with those of squamous epithelial cancer, which often leads to misdiagnosis. This study was to discuss imaging features of Waldeyer's ring in NHL, and its clinical significance.
CT and MRI performances of 149 patients with pathologically diagnosed Waldeyer's ring-NHL were retrospectively analyzed.
Among 149 cases of Waldeyer's ring-NHL, 98 (65.8%) were B-cell origin, and 51 (34.2%) were T-cell or NK/T-cell origin; tonsil was the most vulnerable site followed by nasopharyngeal tonsil and other positions. CT and MRI showed that 81 were focal nodules or masses (commonly in B-cell NHL), 36 were diffuse infiltrating growth (commonly in NK/T-cell NHL), 7 were pure ulcer, and 25 were mixed type. The lesions of focal masses are characterized with even CT density and MRI signals. The lesions usually localized in pharynx-mucosa clearance, but rarely in deep space, such as parapharyngeal space, and in skull-base. Among 78 cases with involved cervical lymph nodes, 64 were B-cell origin, and 14 were NK/T-cell origin (P< 0.05).
Multiple positions and centers origin, huge masses, diffuse infiltration growth, and less involvement in deep space and skull-base are typical imaging features of Waldeyer's ring-NHL; CT and MRI may be helpful to diagnose, clinical classifying, and treating Waldeyer's ring-NHL.
Ai zheng = Aizheng = Chinese journal of cancer 11/2004; 23(11):1325-8.
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ABSTRACT: To study the expression of ER mRNA, presenilin 2 (PS2) and their relationship and clinicopathological significance in the pancreatic carcinoma.
The assay of ER mRNA was detected by in situ hybridization technique and the expression of PS2 was completed by immunohistochemical method of avidin-biotin complex in the 10% neutral formalin-fixed and routinely paraffin-embedded sections of specimen with pancreatic carcinoma.
The positive rate and the score of PS2 were 45.7% and 1.82 +/- 1.34, also those of ER mRNA were 51.4% and 2.08 +/- 1.46 in 35 patients with pancreatic carcinoma. The highly positive correlations were found among the positive rate and the score of ER mRNA and PS2. The positive rate and the score were significantly higher in the cases of well-differentiated adenocarcinoma without metastasis than those in the ones of middle- or poorly-differentiated adenocarcinoma with metastasis.
The expression of ER mRNA or PS2 might reflect the biological behaviors and hormone-dependent status of pancreatic carcinoma. The assay of ER mRNA and meanwhile the detection of PS2 might have important clinical values for better understanding sex-hormone dependence and raising the therapeutic effect of clinical endocrine therapy of the pancreatic carcinoma.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 09/2004; 29(4):438-40.
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Yu Wen,
Yong-guo Li,
Zhu-lin Yang,
Xue-jun Wang,
Hong Wei,
Wei Liu,
Ai-ling Tan,
Xiong-ying Miao,
Qun-wei Wang, Sheng-fu Huang,
E Olavi Kajander,
Neva Ciftcioglu
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ABSTRACT: To find the distribution of nanobacteria in the serum, bile and gallbladder mucosa of cholecystolithiasis patients.
The infection rate of nanobacteria was identified by ELISA in the serum samples from 338 healthy people and 76 patients with cholecystolithiasis (chi(2) = 0.89, P > 0.05). Nanobacteria were cultured from the bile samples in 57 patients with cholecystolithiasis and 18 non-cholelithiasis patients and identified by immunohistochemical staining and TEM (chi(2) = 29.80, P < 0.05). Forty samples of gallbladder mucosa randomly selected from the 57 cholecystolithiasis patients were identified by immunohistochemical staining and compared with the corresponding bile samples.
The infection rate of nanobacteria was 8.0% and 31.6% for the serum samples of the healthy people and cholecystolithiasis patients, respectively. The positive rate of nanobacteria in the bile samples was 61.3% and there was no significant difference in the bile of the cholecystolithiasis patients and the control group (61.4% vs. 61.1%). Fourteen positive patients had infection of nanobacteria in the gallbladder mucosa, submucosa, and calcific field.
The infection rate of nanobacteria was 8% in the serum samples from the healthy people. There are nanobacteria in the serum, bile, and gallbladder mucosa. The infection of the nanobacteria may result in calcification and fibrosis of the gallbladder.
Zhonghua wai ke za zhi [Chinese journal of surgery] 05/2003; 41(4):267-70.
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ABSTRACT: Intraoperative bleeding remains a major concern during mesohepatectomy because of doubled area of cut surface and proximity to important intrahepatic vascular structures. Preliminary extrahepatic exposure and looping of the main hepatic veins with the possibility of clamping them in association with total or partial vascular inflow occlusion, can lead to substantially reducing intraoperative bleeding.
From January 2003 to July 2008, preliminary exposure and looping of the main hepatic veins was performed in 67 patients undergoing mesohepatectomy. Among these patients, mesohepatectomy was performed with clamping of more than one of the main hepatic veins in 47 patients. We report the results obtained in those patients.
Total vascular inflow occlusion with Pringle maneuver or partial vascular inflow occlusion based on an intrahepatic approach was used in all patients. The amount of intraoperative blood loss averaged (580 +/- 308) (range 180 to 4500) ml. No macroscopic tumor residue was encountered. There were no hospital deaths and the morbidity rate was 25.7%. The mean hospital stay was 11.2 days (range, 9-26).
Our study showed that preliminary extrahepatic control of the main hepatic veins was a safe and technically feasible maneuver. During mesohepatectomy, clamping more than one of the main hepatic veins, in association with total or partial vascular inflow occlusion, is efficacious in reducing intraoperative bleeding.
Hepato-gastroenterology 56(96):1730-4. · 0.66 Impact Factor
