Sarangerel Oidovsambuu

Korea Institute of Science and Technology, Sŏul, Seoul, South Korea

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Publications (9)19.77 Total impact

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    Myung Suk Kim, Sue Ji Lim, Sarangerel Oidovsambuu, Chu Won Nho
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    ABSTRACT: Ethnopharmacological relevance Paeonia anomala L. is used in Mongolian traditional medicine to treat various diseases including indigestion, abdominal pain, kidney disorders, inflammation, and female diseases. Aim of the study In this study we examined the effects of Paeonia anomala extract (PAE) and compounds derived from Paeonia anomala on immunoglobulin E (IgE)-mediated type I hypersensitivity responses in vitro. Materials and Methods Degranulation assay, reverse transcription PCR, Enzyme-lined immunosorbent assays, western blot analyses were performed to measure allergic and proinflammatory mediators in IgE-stimulated rat basophilic leukemia (RBL)-2H3 mast cells treated with or without PAE or gnetin H. Results Seventeen compounds were isolated, and β-hexosaminidase release from IgE-stimulated RBL-2H3 mast cells was measured. Of the seventeen isolated compounds, gnetin H, a resveratrol derivative, significantly inhibited β-hexosaminidase release from RBL-2H3 cells with an IC50 value of 0.3 μM. Notably, Gnetin H reduced β-hexosaminidase release at lower concentrations than resveratrol. Furthermore, PAE and gnetin H inhibited histamine secretion, decreased the production and mRNA expression of tumor necrosis factor-α and interleukin-4 and suppressed translocation of nuclear factor κB. PAE and gnetin H also reduced the expression of cyclooxygenase-2 and production of prostaglandin E2. PAE and gnetin H suppressed the phosphorylation of Syk, protein kinase C (PKC)μ, phospholipase Cγ, and the mitogen-activated protein kinases, c-Jun N-terminal kinase, p38, and extracellular signal-regulated kinase. Conclusions These results suggest that PAE and its active compound gnetin H could be promising therapeutic agents for allergic disorders.
    Journal of Ethnopharmacology 05/2014; · 2.94 Impact Factor
  • Myungsuk Kim, Sue Ji Lim, Sarangerel Oidovsambuu, Chu Won Nho
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    ABSTRACT: Paeonia anomala L. is used in Mongolian traditional medicine to treat various diseases including indigestion, abdominal pain, kidney disorders, inflammation, and female diseases. In this study we examined the effects of Paeonia anomala extract (PAE) and compounds derived from Paeonia anomala on immunoglobulin E (IgE)-mediated type I hypersensitivity responses in vitro. Degranulation assay, reverse transcription PCR, Enzyme-lined immunosorbent assays, western blot analyses were performed to measure allergic and proinflammatory mediators in IgE-stimulated rat basophilic leukemia (RBL)-2H3 mast cells treated with or without PAE or gnetin H. Seventeen compounds were isolated, and β-hexosaminidase release from IgE-stimulated RBL-2H3 mast cells was measured. Of the seventeen isolated compounds, gnetin H, a resveratrol derivative, significantly inhibited β-hexosaminidase release from RBL-2H3 cells with an IC50 value of 0.3μM. Notably, Gnetin H reduced β-hexosaminidase release at lower concentrations than resveratrol. Furthermore, PAE and gnetin H inhibited histamine secretion, decreased the production and mRNA expression of tumor necrosis factor-α and interleukin-4 and suppressed translocation of nuclear factor κB. PAE and gnetin H also reduced the expression of cyclooxygenase-2 and production of prostaglandin E2. PAE and gnetin H suppressed the phosphorylation of Syk, protein kinase C (PKC)μ, phospholipase Cγ, and the mitogen-activated protein kinases, c-Jun N-terminal kinase, p38, and extracellular signal-regulated kinase. These results suggest that PAE and its active compound gnetin H could be promising therapeutic agents for allergic disorders.
    Journal of ethnopharmacology 05/2014; · 2.32 Impact Factor
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    ABSTRACT: The antioxidant properties and phenolic profiles were firstly investigated in this paper on the red pepper leaves of three cultivars, Blackcuban (BCPL), Hongjinju (HPL), and Yeokgang-hongjanggun (YHPL). Of the ethanol extract of the three cultivars, BCPL showed potent antioxidant activities against the 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH) and the 2,2-azinobis (3-ethyl benzothiazoline-6-sulfonic acid) (ABTS) radical. Nine antioxidative compounds from the red pepper leaves were isolated and identified as one polyamine phenolic conjugate, N-caffeoyl putrescine (1); three chlorogenic acid derivatives, 5-O-caffeoyl quinic acid (2), 5-O-caffeoyl quinic acid methyl ester (4), 5-O-caffeoyl quinic acid butyl ester (9); one anthocyanin, delphinidin-3-[4-trans-coumaroyl-L-rhamnosyl-(1→6)-glucopyranoside]-5-O-gluco-pyranoside (3); and four flavone glycosides, luteolin-7-O-apiofuranosyl-(1→2)-glucopyranoside (5), luteolin-7-O-glucopyranoside (6), apigenin 7-O-apiofuranosyl(1→2)-glucopyranoside (7), apigenin-7-O-glucopyranoside (8). 1 and 3 had the greatest potential for radical scavenging activity and HepG2 cells protecting effect against oxidative stress. BCPL exhibited the highest content of 1 and 3. The BCPL of three cultivars may be considered an good source of antioxidants.
    Journal of Agricultural and Food Chemistry 10/2013; · 3.11 Impact Factor
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    ABSTRACT: We investigated the protective effects of Gymnaster koraiensis against oxidative stress-induced hepatic cell damage. We used two different cytotoxicity models, i.e., the administration of tert-butyl hydroperoxide (t-BHP) and acetaminophen, in HepG2 cells to evaluate the protective effects of G. koraiensis. The ethyl acetate (EA) fraction of G. koraiensis and its major compound, 3,5-di-O-caffeoylquinic acid (DCQA), exerted protective effects in the t-BHP-induced liver cytotoxicity model. The EA fraction and DCQA ameliorated t-BHP-induced reductions in GSH levels and exhibited free radical scavenging activity. The EA fraction and DCQA also significantly reduced t-BHP-induced DNA damage in HepG2 cells. Furthermore, the hexane fraction of G. koraiensis and its major compound, gymnasterkoreayne B (GKB), exerted strong hepatoprotection in the acetaminophen-induced cytotoxicity model. CYP 3A4 enzyme activity was strongly inhibited by the extract, hexane fraction, and GKB. The hexane fraction and GKB ameliorated acetaminophen-induced reductions in GSH levels and protected against cell death. [BMB Reports 2013; 46(10): 513-518].
    BMB reports 10/2013; 46(10):513-8. · 1.63 Impact Factor
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    ABSTRACT: Three new chalcones, 3,2'-dihydroxy-4,3'-dimethoxychalcone-4'-glucoside (1), 4'-O-(2'''-O-caffeoyl)2',3',3,4-tetrahydroxychalcone (2), and 2',4',3-trihydroxy-3',4-dimethoxychalcone (3), along with five known phenolics, were isolated from Coreopsis lanceolata flowers. The structures of the new compounds were elucidated by extensive spectroscopic methods including NMR and MS. The three new chalcones showed a good in vitro HepG2 cell protecting effect against tert-butylhydroperoxide-induced oxidative stress.
    Planta Medica 03/2013; 79(3-4):295-300. · 2.35 Impact Factor
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    ABSTRACT: The fruit and root parts of Paeonia anomala L. are used for the treatment of many kinds of disorders in Mongolian traditional medicine. The protective effect of a fruit extract from P. anomala against tert-butylhydroperoxide-induced cell damage was evaluated in human hepatoma HepG2 cells and compared to that of a root extract from P. anomala on the basis of cell viability, generation of intracellular reactive oxygen species, cellular total glutathione concentration, and anti-genotoxicity. The fruit extract of P. anomala showed excellent protection against the oxidative stress when compared to the root extract, through free radical scavenging, enhancing cellular glutathione concentration, and inhibiting DNA damage. Chemical constituents in the fruit extract of P. anomala were investigated and two novel compounds, 2-hydroxy-6-methoxy-4-O-(6'-O-α-L-arabinofuranosyl-β-D-glucopyranosyl)acetophenone (1) and 3,3'-di-O-methyl-4-O-(3''-O-galloyl-β-D-glucopyranosyl)ellagic acid (2), along with 18 other known compounds were identified. Compound 2 showed better cytoprotection against tert-butylhydroperoxide than compound 1. Among other compounds isolated from the fruit extract, ellagic acid, methyl gallate, ethyl gallate, fischeroside B, and quercetin derivatives showed potent protective effects against tert-butylhydroperoxide-induced oxidative stress via inhibiting reactive oxygen species generation and increasing total glutathione levels in HepG2 cells.
    Planta Medica 01/2013; 79(2):116-22. · 2.35 Impact Factor
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    ABSTRACT: Handaeri-gomchi (Ligularia fischeri var. spiciformis Nakai) (LF) is well known as a medicinal plant in Korea, especially to treat a variety of human disease including hepatic function failure. In this study, we examined the activity of LF extract against the liver injuries and oxidative stress by chronic alcohol in Spargue-Dawley rats. Severe liver damage caused by alcohol intake with increasing activity of hepatic markers was decreased in the group of rats fed LF extract. The results were confirmed through hematoxylin and eosin staining. Antioxidative capacity was decreased by alcohol but it was recovered by LF extract. Elevated indicators of oxidative stress by chronic alcohol were diminished in the group of LF extract. Furthermore, LF extracts increased antioxidative capacity and the activity of antioxidant enzymes. In conclusion, LF extract has a protective effect against chronic alcohol hepatotoxicity, suggesting it could be developed as a functional food or medicine for protection of liver disease.
    Food science and biotechnology 12/2011; 20(6):1655-1661. · 0.70 Impact Factor
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    ABSTRACT: Improvement of liver function is one of the most popular commercial health claims of functional foods in Asian countries, including Korea. After examining the potential of several traditional Korean wild vegetables for enhancing liver function, we found that Youngia denticulata Kitam. has strong hepatoprotective effects against oxidative stress induced by tert-butylhydroperoxide (t-BHP). We are the first to report that the extract and ethyl acetate fractions of Y. denticulata have radical scavenging activities and inhibit oxidative stress-induced cell death and DNA damage in HepG2 cells. The extract and ethyl acetate fractions significantly decreased cellular reactive oxygen species production and apoptosis induced by t-BHP in HepG2 cells. In addition, they prevented the depletion of cellular glutathione, which is an important defense molecule against oxidizing xenobiotics. Chlorogenic acid and 3,5-dicaffeoylquinic acid were found to be major active components responsible for the activity of Y. denticulata and could serve as marker compounds for standardization. These data suggest that Y. denticulata could be promoted as a potential antioxidative functional food candidate, particularly for hepatoprotection against oxidative stress.
    Journal of medicinal food 09/2011; 14(10):1198-207. · 1.39 Impact Factor
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    ABSTRACT: In the present study, we isolated a polyacetylene, gymnasterkoreayne B (GKB), from Gymnaster koraiensis and investigated the effect of GKB on the protection from oxidative stress-induced cytotoxicity through induction of the expression of cellular defense enzymes. GKB induced mRNA expression and enzyme activity of NAD(P)H:quinone oxidoreductase (NQO1) in vitro and in vivo, and potently increased expression of many cellular defense genes including glutathione-S-transferases, UDP-glucuronosyltransferase, and glutathione reductase (GSR) in normal rat liver. The nuclear factor erythroid 2-related factor 2 (Nrf2) which is known to induce various antioxidant and cytoprotective genes, and the genes containing the antioxidant response element (ARE), including NQO1, hemeoxygenease-1, GSR were induced by GKB in HepG2 human hepatocarcinoma cells. Pre-treatment of the cells with GKB accelerated the production of glutathione and mitigated menadione-induced cytotoxicity in HepG2 cells. Taken together, we found that GKB was a novel inducer of phase II detoxification enzymes and cellular defense enzymes, resulting in protection of the cells from oxidative stress and hepatotoxicity through regulation of detoxifying and antioxidant systems.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 11/2010; 48(11):3035-41. · 2.99 Impact Factor