Ryan D Rutledge

Vanderbilt University, Nashville, MI, United States

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Publications (6)17.96 Total impact

  • Ryan D. Rutledge · David W. Wright ·

    Encyclopedia of Inorganic Chemistry, 03/2009; , ISBN: 9780470862100
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    ABSTRACT: Human respiratory syncytial virus (HRSV) is the leading cause of acute lower respiratory tract infection in children under 5 years of age, and acute lower respiratory tract infection is the leading cause of death worldwide. During the cold season, hospitals become overcrowded, presenting the need for cheap, rapid, accurate diagnostics. To this end, a quartz crystal microbalance (QCM) immunosensor has been developed by our lab. It utilizes the commercially available pharmaceutical IgG Palivizumab (from AstraZeneca, MedImmune) immobilized by Protein G. In order to calibrate this instrument, a stock solution of HRSV is necessary. In order to work away from live viruses, which present a safety risk to medical personnel and patients, biomimetic gold monolayer protected clusters (MPCs) presenting peptide epitopes can be employed for calibration. A gold MPC presenting a HRSV fusion protein antigenic site A peptide has shown promising results for use as a calibrant. Analysis using the QCM immunosensor shows Langmuirian concentration dependence and a reasonable binding affinity constant (Ka ≈ 2 x 105). Gold MPCs presenting the same epitope in a loop conformation via bidentate attachment are currently being investigated, and are expected to have an enhanced affinity.
    60th American Chemical Society Southeast Regional Meeting; 11/2008
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    Sarah L Sewell · Ryan D Rutledge · David W Wright ·
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    ABSTRACT: Biomimetic synthesis is emerging as an advantageous alternative to the harsh synthetic conditions traditionally used in metal oxide syntheses techniques. Silaffins, proteins from the C. fusiformis diatom, form silica in an aqueous environment under benign conditions. Amine terminated PAMAM and PPI dendrimers are effective mimics of silaffins and other silica precipitating polyamines. We have expanded the scope of dendrimer mediated metal oxide formation to include titanium dioxide, a photocatalyst, and germanium dioxide, a blue photoluminescent material. The nanoparticles were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (IR), and X-ray diffraction patterns (XRD). A variable temperature XRD analysis of TiO(2) nanoparticles was conducted to study the transition from anatase to rutile. TiO(2) nanoparticles synthesized in phosphate buffer showed a 200 degrees C decrease in the anatase to rutile transition temperature relative to TiO(2) templated in water. XRD analysis of GeO(2) nanoparticles in either water or phosphate buffer reveal crystalline alpha-phase germanium oxide. To our knowledge, this is the first report of the synthesis of crystalline GeO(2) under ambient conditions.
    Dalton Transactions 08/2008; 29(29):3857-65. DOI:10.1039/b802842g · 4.20 Impact Factor
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    Ryan D Rutledge · Brian J Huffman · David E Cliffel · David W Wright ·
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    ABSTRACT: An antigenic mimic of the Ebola glycoprotein was synthesized and tested for its ability to be recognized by an anti-Ebola glycoprotein antibody. Epitope-mapping procedures yielded a suitable epitope that, when presented on the surface of a nanoparticle, forms a structure that is recognized by an antibody specific for the native protein. This mimic-antibody interaction has been quantitated through ELISA and QCM-based methods and yielded an affinity (K(d) = 12 × 10(-6) M) within two orders of magnitude of the reported affinity of the native Ebola glycoprotein for the same antibody. These results suggest that the rational design approach described herein is a suitable method for the further development of protein-based antigenic mimics with potential applications in vaccine development and sensor technology.
    Journal of Materials Research 02/2008; 23(12):3161-3168. DOI:10.1557/JMR.2008.0384 · 1.65 Impact Factor
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    ABSTRACT: Ultrasonication of toluene solutions of the heteropolynuclear cluster complex, Pt3Fe3(CO)15, in the presence of oleic acid and oleylamine affords surface-capped fcc FePt nanoparticles having an average diameter of ca. 2 nm. Self-assembled arrays of these nanoparticles on oxidized Si wafers undergo a fcc-to-fct phase transition at 775 degrees C to form ferromagnetic FePt nanocrystals ca. 5.8 nm in diameter well dispersed on the Si wafer surface. Room-temperature coercivity measurements of these annealed FePt nanoparticles confirm a high coercivity of ca. 22.3 kOe. Such high coercivity for fct FePt nanoparticles might result from use of a heterpolynuclear complex as a single-source precursor of Fe and Pt neutral atoms or from use of ultrasonication to form fcc FePt nanoparticles under conditions of exceptionally rapid heating. Experiments to determine the critical experimental conditions required to achieve such high room-temperature coercivities in ferromagnetic nanoparticles are underway.
    Journal of the American Chemical Society 12/2006; 128(44):14210-1. DOI:10.1021/ja0633868 · 12.11 Impact Factor