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Publications (25)19.4 Total impact

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    ABSTRACT: 1. The objectives of the study were to detect polymorphism in the coding region of the IGF1 gene, explore the expression profile and estimate association with growth traits in indigenous and exotic chickens. 2. A total of 12 haplotypes were found in Cornish, control layer and Aseel breeds of chicken in which the h1 haplotype was most frequent. 3. Nucleotide substitutions among haplotypes were found at 21 positions in the IGF1gene in which 4 substitutions resulted in non-synonymous mutations in the receptor binding domain of the IGF1 protein. 4. The haplogroup showed a significant effect on body weight at 24 and 42 d of age in the control layer line, body weight at 42 d and daily weight gain between 29 to 42 d in the control broiler line, daily weight gain between 29 to 42 d in Cornish, and body weights at 42 d as well as daily weight gain between 29 to 42 d in Aseel birds. 5. IGF1 expression varied among the breeds during embryonic and post-hatch periods. The expression among the haplogroups varied in different chicken tissues. The effect of haplogroups on myofibre number in pectoral muscle was non-significant, although there was significant variation in numbers between d 1 and 42, and between broiler and layer lines. 6. It was concluded that the coding region of the IGF1 gene was polymorphic, expressed differentially during the pre-hatch and post-hatch periods, and haplogroups showed significant association with growth traits in chicken.
    British Poultry Science 06/2015; DOI:10.1080/00071668.2015.1041098 · 0.78 Impact Factor
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    ABSTRACT: A study was conducted to characterize myostatin gene in broiler and layer chicken and to explore mRNA expression profile in these two varieties of chicken. The myostatin cDNAs of broiler and layer varieties were cloned and sequenced. The total length of the cDNA was 1128 bp. The differences of nucleotides between PB-1 broiler and IWI layer were C > 65 > T, C > 306 > T and C > 1094 > T while those between CB broiler and IWI layer were C > 65 > T, C > 195 > G, G > 234 > A, C > 306 > T, T > 939 > C, C > 961 > T, G > 966T and C > 1094 > T. The amino acid differences of myostatin protein between PB-1 and IWI strains were alanine > 22 > valine and proline > 365 > leucine while those between CB and IWI strains were alanine > 22 > valine, histidine > 321 > tyrosine and proline > 365 > leucine. The phylogenetic study revealed closeness of PB-1 and control broiler forming a cluster, which was also closely related to IWI layer chicken formed a separate cluster. The gene was cloned and expressed in E. coli. The gene expression profile in muscle was different between broiler and layer strains. Between two broiler strains, the pattern of expression was similar. Between IWI layer and either PB-1 or CB broilers, differences in expression was found at different time points, particularly at second, fourth and seventh weeks of age. The myostatin expression was significantly associated with body weights in chicken. It is concluded that myostatin gene sequences varied at nucleotide as well as amino acid level between broiler and layer chicken varieties and the gene also expressed differentially in these two varieties.
    Molecular Biology Reports 12/2014; 42(2). DOI:10.1007/s11033-014-3753-x · 1.96 Impact Factor
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    ABSTRACT: India although has achieved self sufficiency in food grains, it has to solve the problems of protein-energy malnutrition for millions of people. Presently, poultry industry has emerged as a big hope, which can meet not only the protein requirement to millions but can provide employment and thereby, livelihood security. India is the 3rd largest producer of eggs and holds 5th position in poultry meat production in the world. Developments like emergence of molecular markers, structural and functional genomics, bioinformatics etc. in the field of the biotechnology have provided ample opportunity to peep into the molecular architecture of the individual birds. Improvement of poultry birds all together can be achieved through conventional breeding approach, but amalgamation of conventional and molecular approach can generate further improvement of birds in sustainable manners, which in turn augment productivity of the poultry very rapidly and efficiently bypassing the impact of climate change being faced by the whole world today.
    Proceedings of the National Academy of Sciences, India - Section B: Biological Sciences 12/2014; 84(4). DOI:10.1007/s40011-014-0306-y · 0.40 Impact Factor
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    ABSTRACT: Coloured broiler breeders are becoming popular in recent years because of the preference for a range of poultry products as well as the welfare issues in the poultry industry. The female broiler breeders are continuously improved for various economic traits. Amongst these, egg quality trait is very essential for the production of healthy and viable chicks. This study investigates the egg quality parameters in a synthetic coloured broiler breeder female line at different age groups. The study revealed that the egg quality is optimum for setting the eggs for hatching from 40 week onwards as the egg size and internal egg parameters attain the minimum standards for the production of a healthy chick.
    Journal of Applied Animal Research 08/2014; 43(1):10-14. DOI:10.1080/09712119.2014.883319 · 0.48 Impact Factor
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    ABSTRACT: A study was conducted to assess genetic heterogeneity among 14 chicken populations using 16 microsatellite markers. All the microsatellite loci except MCW048 were polymorphic. The number of alleles varied from 2 to 6. The polymorphic information content of the microsatellite markers varied from 0.21 to 0.87. The average observed number of alleles across the microsatellites varied from 3 to 3.8, while effective number of alleles varied from 1.9 to 3.0. The average observed and expected heterozygosities were in the range of 0.3-0.6 and 0.4-0.6, respectively. The markers, MCW049, MCW044, MCW041, ADL176, MCW059, ADL136 and MCW110 showed heterozygosity excess and ADL158, MCW043, ADL102, MCW014, ADL023, ADL210 and MCW007 markers revealed heterozygosity deficiency in the populations. The genetic distance between layer and broiler lines was found to be high ranging from 0.22 to 0.38. The Indigenous populations were more closely related to broiler lines than layer ones. In conclusion, it is stated that Indigenous, broiler and layer chicken populations were heterogeneous in nature and Indigenous chicken populations were distantly related from the broiler and layer chicken populations.
    Journal of Applied Animal Research 05/2014; DOI:10.1080/09712119.2014.963097 · 0.48 Impact Factor
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    ABSTRACT: Chickens (Gallus gallus) were the first avian species selected for whole genome sequencing because of their economic value, use as a food source, livelihood security and research importance. Any living organism contains a galaxy of genes which express all the phenotypes or characters by encoding proteins and peptides, and playing regulatory roles in the biological system. Functional genomics in turn, is a multidisciplinary approach to identify and demonstrate the functional roles of genes and other regulatory molecules such as microRNA and CpG methylation in biological pathways. In the last two decades, the chicken genome database has made significant advancements in accruing large amounts of genomic information through employing advanced bio-informatic tools. Several techniques such as cDNA microarray, serial analysis of gene expression, massively parallel signature sequencing, cDNA subtractive hybridisation and next generation sequencing have been utilised to investigate the genome-wide expression profile instead of revealing expression pattern of one or a few genes in various avian species. Expressed sequence tag or cDNA sequences are the key factors for identification of novel genes and understanding the complex molecular cascades of ontology. A large-scale cDNA library has been constructed from embryonic and adult tissues and consequently identified the presence of about 19,000 functional genes in chickens. The micro RNAs play crucial role in gene expression and to date, approximately 496 micro RNAs have been characterised. The non-coding RNA alters gene expression involved in cellular process, by modulating the chromatin architecture, transcription, RNA splicing, editing, translation and turnover. Functional genomics studies have been extensively used to identify genes associated with several production traits, immuno-genetic mechanism, host-pathogen interaction, pathogen biology etc. Nutrigenomics have determined the genomic mechanism involved in feed utilisation, metabolism and cholesterol synthesis etc., which ultimately reveal potential applications for improving the nutritional efficiency of birds. This review discusses the tools and utility of functional genomics approaches in chicken.
    World's Poultry Science Journal 03/2014; 70(01):45-56. DOI:10.1017/S004393391400004X · 1.16 Impact Factor
  • T K Bhattacharya, R N Chatterjee
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    ABSTRACT: An experiment was carried out on myostatin gene with the objectives of identification of polymorphism in the myostatin gene and estimation of the effect of polymorphism on growth traits in chickens. Single-stranded conformation polymorphism followed by sequencing was performed to reveal polymorphism of the gene. A total of 13 haplotypes were observed across 3 chicken lines (PB-1 and CB as broiler lines and IWI as the layer line). Myostatin haplogroups had a significant effect on BW at 28, 42, and 49 d of age in the PB-1 line. The significant association of haplogroups was observed with BW at d 14 and 49 in the CB line. In the IWI layer line, the myostatin gene was polymorphic but had no significant association with growth traits. It is concluded that the myostatin gene was polymorphic and had a significant effect on growth traits in broiler chickens.
    Poultry Science 04/2013; 92(4):910-5. DOI:10.3382/ps.2012-02736 · 1.67 Impact Factor
  • The Indian veterinary journal 11/2012; 89(11):34. · 0.06 Impact Factor
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    T K Bhattacharya, R N Chatterjee, M Priyanka
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    ABSTRACT: The Pit-1 gene is involved in regulation of muscle growth through controlling the expression of growth hormone, prolactin, and transforming growth factor-β genes in chicken. The objectives of the study were to explore polymorphisms of the Pit-1 gene and to estimate the effect of these polymorphisms on growth traits in PB-1 and control (broiler strain) and IWI (layer strain) chickens. Single-stranded conformation polymorphism followed by sequencing was performed to reveal polymorphisms of the gene. In total, 10 haplotypes were found across the lines. The mRNA expression of Pit-1 varied among haplogroups and had a significant effect on BW and growth rates. The haplogroups showed a significant effect on BW in wk 7 in PB-1 chickens. In control chickens there was a significant effect at d 1 and in wk 2 and 7, and in IWI strains, there was a significant effect at d 1 and wk 6 and 7. The significant association of haplogroups and growth rate was found between 0 and 2 wk in control and between 0 and 2 and 6 and 7 wk in IWI strains. It was concluded that the Pit-1 gene is polymorphic and has a significant effect on growth traits in chickens.
    Poultry Science 05/2012; 91(5):1057-64. DOI:10.3382/ps.2011-01990 · 1.67 Impact Factor
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    ABSTRACT: A study was conducted to evaluate the effect of feeding different metabolizable energy (ME) and crude protein (CP) levels on performance of Aseel chicken during 0 to 8 weeks of age (Juvenile phase). At 1 day old, 432 chicks were randomly distributed into nine groups. Each group had 48 chicks distributed into eight replicates with six birds in each. Maize-soybean meal-based diets with three ME levels (2,400, 2,600 and 2,800 kcal/kg) and three CP levels (16%, 18% and 20%) were fed to birds in a 3 × 3 factorial design. Different ME levels had significant effect on body weight gain (BWG), feed intake and feed conversion ratio (FCR). Birds fed diet with 2,400 kcal/kg ME had significantly lower BWG (P < 0.004), lower shank length (P < 0.0007), higher feed intake (P < 0.0001) and poor FCR (P < 0.0001) than those fed diet with either 2,600 or 2,800 kcal/kg ME. Energy efficiency ratio was not influenced by ME, CP or their interaction. However, protein efficiency ratio was significantly higher at higher ME levels and lower at higher CP levels. There was significant effect of ME, CP and their interaction on serum protein and cholesterol levels. However, they made no significant effect on antibody titre against New Castle disease vaccine. The study concluded that provision of 2,600 kcal/kg ME and 16% CP would be ideal for optimum growth of Aseel birds during juvenile phase. However, to obtain better FCR, feeding Aseel birds with diet having 2,800 kcal/kg ME and 16% CP would be ideal.
    Tropical Animal Health and Production 03/2012; 44(7):1653-8. DOI:10.1007/s11250-012-0120-6 · 0.97 Impact Factor
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    ABSTRACT: A study was designed to explore genetic variability of partial promoter and exon 1 of pit-1 gene and its effect on immune competence traits in White Leghorn chicken. Single stranded conformation polymorphism technique followed by sequencing was employed to detect polymorphism of the gene. Three genotypes (AA, AB and BB) were observed in White Leghorn chicken where two alleles (A and B) were found. AB genotype and A allele were predominant in the population. Different demographic parameters estimated in the White Leghorn chicken lines indicated the presence of genetic divergence among the lines. The pit-1 mRNA expression varied among the genotypes where genotype BB expressed higher than genotype AA and AB. The genotypes as well as alleles showed significant effect (P < 0.05) on sheep RBC titre in White Leghorn chicken.
    Journal of Applied Animal Research 12/2011; 39(4):298-302. DOI:10.1080/09712119.2011.607889 · 0.48 Impact Factor
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    ABSTRACT: Single strand conformation polymorphism analysis and DNA sequencing was performed in White Leghorn hens to explore the polymorphisms present in the promoter of the prolactin gene. The effects of different genotypes on egg production and quality traits were determined, and expression of the prolactin gene in different genotypes was quantified by real time-PCR. Five genotypes and four alleles at each of two Fragments of the promoter were found, of which the FG genotype in Fragment 1 and the PQ genotype in Fragment 2 were the most predominant genotypes. The genotypes of Fragment 1 had significant effects (P < 0·05) on Haugh unit, albumen weight, albumen percentage and shell percentage at 40 weeks of age; egg weight and yolk index at 52 weeks of age; and egg weight at 64 weeks of age. Prolactin expression in the genotypes of Fragment 1 differed significantly and GH genotyped birds had the highest level of expression. The genotypes of Fragment 2 did not show any significant differences of expression. It was concluded that the prolactin gene promoter was highly polymorphic, and had significant effects on egg quality traits in White Leghorn hens.
    British Poultry Science 10/2011; 52(5):551-7. DOI:10.1080/00071668.2011.617727 · 0.78 Impact Factor
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    ABSTRACT: The pituitary hormone prolactin has a wide variety of functions involving growth, behavioral, and ovarian activities in chickens. The objectives of the present study were to identify polymorphisms in the prolactin promoter and estimate their effects on growth traits in White Leghorn chickens. Among 28 haplotypes found, the h1 haplotype was predominant. Body weight at 16 and 64 weeks and age at sexual maturity were significantly associated with haplotype combinations (P < 0.05). The h1/h1 haplogroup showed the highest body weight at 16 weeks of age, and h1/h7 was the highest at 64 weeks. The lowest age at sexual maturity was found in birds with the h1/h6 haplotype combination, and mRNA expression of prolactin was lowest in h1/h4 birds and highest in h1/h5 birds. The prolactin promoter was highly polymorphic and had significant associations with growth traits in White Leghorn chickens.
    Biochemical Genetics 06/2011; 49(5-6):385-94. DOI:10.1007/s10528-010-9415-3 · 0.82 Impact Factor
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    ABSTRACT: Expression of prolactin hormone is a crucial event in regulating egg production in chickens for which promoter plays the vital role in expressing the prolactin gene. The objective of the present study was to identify haplotypes in the prolactin promoter and their effects on egg production and egg quality traits in White Leghorn chicken. Single stranded conformation polymorphism followed by sequencing was conducted to explore polymorphism at 561 bp promoter of prolactin gene. The effect of haplotype combinations on egg production and quality traits were estimated following general linear model technique. The expression of prolactin by different haplogroups was quantified by qPCR. Total 28 haplotypes were found in White Leghorn chicken of which h1 haplotype possessed the highest frequency of 0.46 and h8, h14, h16, h25, h26, and h28 haplotypes had the lowest frequency (0.1%). The egg production up to 52 and 64 weeks of age were found to be significantly (p < 0.05) associated with haplotype combinations where the highest 52-w (52 weeks) egg production was found in animals with h1/h22 combination and the lowest production was observed in the birds with h1/h2 haplogroup. The haplotype combinations had the significant effect (p < 0.05) on Haugh Unit, yolk index and albumen weight at 40 weeks of age; Haugh Unit and albumen weight at 52 weeks of age and Haugh unit, yolk weight and yolk percentage at 64 weeks of age. The prolactin expression in h1/h22 birds was found to be the lowest and in h1/h5 birds to be the highest. The prolactin expression showed significant effect on 52-w egg production and albumin weight at 52 weeks age. In conclusion, it may be stated that the prolactin promoter was highly polymorphic and had the significant association with egg production and quality traits in White Leghorn chicken.
    Animal Biotechnology 04/2011; 22(2):71-86. DOI:10.1080/10495398.2011.555680 · 0.64 Impact Factor
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    ABSTRACT: The objective was to characterize polymorphisms at the 5'-UTR region of the prolactin gene, and determine their association with egg production and egg quality traits in White Leghorn chickens. The study was conducted on four strains of White Leghorn chickens, namely IWH, IWI, IWK, and layer control. Overall, there were three alleles (designated A, B, and C) and five genotypes, with genotypic frequencies of 0.09, 0.75, 0.07, 0.02, and 0.07 for AA, AB, AC, BB, and BC, respectively. There were significant differences among genotypes for egg production up to 52 and 64 wk of age, with maximal egg yields for genotypes AA and AC (144.5 ± 5.06 and 143.2 ± 4.67 eggs, respectively). Furthermore, there were significant differences among genotypes for egg quality traits, including egg weight and Haugh unit at 40 wk of age, Haugh unit at 52 wk, and yolk color index and Haugh unit at 64 wk. Birds with AA or AC genotypes had the best egg quality traits. On the contrary, these genotypes had the lowest prolactin expression, whereas this expression was highest in birds with the BB genotype. In conclusion, polymorphisms at the 5'-UTR of prolactin gene were significantly associated with egg production and egg quality traits in White Leghorn chickens.
    Theriogenology 03/2011; 75(4):655-61. DOI:10.1016/j.theriogenology.2010.10.005 · 1.85 Impact Factor
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    ABSTRACT: The juvenile growth and carcass traits were evaluated in a 4 × 4 full diallel crossing involving four colored broiler chicken lines viz., Naked neck (NN), Dwarf, Punjab Broiler-1 (PB-1), and Punjab Broiler-2 to study the performance and crossbreeding parameters. The data on 2,280 chicks were analyzed using least squares techniques to assess the effect of genetic group, and the significant traits were further analyzed for crossbreeding parameters. Genetic group had significant influence on the body weights and carcass traits. The cross of PB-1 × NN recorded significantly (p ≤ 0.05) higher body weight at 6 weeks of age. The data revealed that general combining ability (GCA), specific combining ability (SCA), maternal ability (MA), sex-linked effect (SE), and purebred effects significantly influenced the growth and carcass traits in the broiler crosses. The crossbred PB-1 × NN showed positive heterosis for growth as well as carcass traits. The results suggest that GCA, SCA, SE, and MA are important in the inheritance of the body weights and carcass traits indicating the presence of both additive and nonadditive genetic variation along with reciprocal effects. Therefore, pure line selection along with development of specialized sire and dam lines followed by crossing will improve the growth performance and benefit the poultry farmers. It is concluded that PB-1 as a male line and NN as a female line performed significantly higher, therefore, may be used for improving the performance of colored broilers.
    Tropical Animal Health and Production 03/2011; 43(6):1129-36. DOI:10.1007/s11250-011-9812-6 · 0.97 Impact Factor
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    ABSTRACT: A study was conducted to explore polymorphism at 5′ flanking region of prolactin gene and to estimate the effect of polymorphism on egg quality traits in naked neck chickens. Single-stranded conformation polymorphism technique was employed to detect polymorphism in the gene. Three genotypes (AA, AB and AC) were observed in naked neck chicken of which the predominant genotype was AB. The predominant and least frequent alleles in the population were A and C, respectively. The sequence alignment study revealed a number of nucleotide substitutions at different positions of the alleles. Genotypes showed significant effect (P < 0.05) on Haugh unit and yolk index where birds with AB and AC genotypes were the best performers. In conclusion, it may be inferred that 5′ flanking region of prolactin gene was polymorphic with having significant effect on some egg quality traits in the naked neck chicken.
    Journal of Applied Animal Research 03/2011; 39(1):72-76. DOI:10.1080/09712119.2011.565224 · 0.48 Impact Factor
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    ABSTRACT: In the present experiment, the expression profile of Toll-like receptor mRNA in indigenous and pure line chickens was studied. The expression of TLR3, TLR4, TLR5 and TLR7 were quantified in heterophils of Aseel, Kadaknath, Naked neck, Dwarf and White Leghorn lines by Quantitative Real-time PCR. White Leghorns expressed significantly (P < 0.01) higher levels of TLR3 mRNA compared to other lines. TLR4 and TLR5 mRNA were significantly highly expressed in Kadaknath line. Among the TLRs investigated TLR5 was more expressed in all lines studied. TLR7 was highly expressed in indigenous chicken Aseel and Kadaknath than other lines. Dwarf chicken expressed significantly (P < 0.01) lower levels of all TLRs investigated. On the basis of the present study we conclude that the differential expression of TLR mRNA in the heterophils of indigenous and other chicken breeds might contribute to their variable disease resistance/susceptibility.
    Veterinary Research Communications 10/2010; 34(7):633-9. DOI:10.1007/s11259-010-9431-0 · 1.36 Impact Factor
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    ABSTRACT: To measure genetic relatedness between populations, for breeding purposes, we analyzed 170 birds from six crossbred populations of three pure lines of White Leghorn chickens, using 14 microsatellite markers. All the microsatellites were polymorphic, with 2-6 alleles. The mean number of alleles per locus was 3.21. The effective number of alleles varied from 1.14 to 3.94. The observed heterozygosity varied from 0.133 to 1.00, with a mean of 0.748. The F (IS) values were mostly negative, with an average of -0.345. The mean F (ST) value was 0.056. The Nm values ranged from 1.91 to 42.17. The highest genetic identity was observed between IWI x IWK and IWK x IWI. The relation between any two groups of crosses was more than 85%. The results suggest that the crossbred populations were very closely related.
    Biochemical Genetics 10/2010; 48(9-10):727-36. DOI:10.1007/s10528-010-9355-y · 0.82 Impact Factor
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    Journal of Genetics 08/2010; 89(2):e33-7. DOI:10.1007/s12041-011-0006-9 · 1.01 Impact Factor