Are you R N Chatterjee?

Claim your profile

Publications (19)14.09 Total impact

  • T K Bhattacharya, R N Chatterjee
    [Show abstract] [Hide abstract]
    ABSTRACT: An experiment was carried out on myostatin gene with the objectives of identification of polymorphism in the myostatin gene and estimation of the effect of polymorphism on growth traits in chickens. Single-stranded conformation polymorphism followed by sequencing was performed to reveal polymorphism of the gene. A total of 13 haplotypes were observed across 3 chicken lines (PB-1 and CB as broiler lines and IWI as the layer line). Myostatin haplogroups had a significant effect on BW at 28, 42, and 49 d of age in the PB-1 line. The significant association of haplogroups was observed with BW at d 14 and 49 in the CB line. In the IWI layer line, the myostatin gene was polymorphic but had no significant association with growth traits. It is concluded that the myostatin gene was polymorphic and had a significant effect on growth traits in broiler chickens.
    Poultry Science 04/2013; 92(4):910-5. · 1.52 Impact Factor
  • The Indian veterinary journal 11/2012; 89(11):34. · 0.05 Impact Factor
  • Source
    T K Bhattacharya, R N Chatterjee, M Priyanka
    [Show abstract] [Hide abstract]
    ABSTRACT: The Pit-1 gene is involved in regulation of muscle growth through controlling the expression of growth hormone, prolactin, and transforming growth factor-β genes in chicken. The objectives of the study were to explore polymorphisms of the Pit-1 gene and to estimate the effect of these polymorphisms on growth traits in PB-1 and control (broiler strain) and IWI (layer strain) chickens. Single-stranded conformation polymorphism followed by sequencing was performed to reveal polymorphisms of the gene. In total, 10 haplotypes were found across the lines. The mRNA expression of Pit-1 varied among haplogroups and had a significant effect on BW and growth rates. The haplogroups showed a significant effect on BW in wk 7 in PB-1 chickens. In control chickens there was a significant effect at d 1 and in wk 2 and 7, and in IWI strains, there was a significant effect at d 1 and wk 6 and 7. The significant association of haplogroups and growth rate was found between 0 and 2 wk in control and between 0 and 2 and 6 and 7 wk in IWI strains. It was concluded that the Pit-1 gene is polymorphic and has a significant effect on growth traits in chickens.
    Poultry Science 05/2012; 91(5):1057-64. · 1.52 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A study was conducted to evaluate the effect of feeding different metabolizable energy (ME) and crude protein (CP) levels on performance of Aseel chicken during 0 to 8 weeks of age (Juvenile phase). At 1 day old, 432 chicks were randomly distributed into nine groups. Each group had 48 chicks distributed into eight replicates with six birds in each. Maize-soybean meal-based diets with three ME levels (2,400, 2,600 and 2,800 kcal/kg) and three CP levels (16%, 18% and 20%) were fed to birds in a 3 × 3 factorial design. Different ME levels had significant effect on body weight gain (BWG), feed intake and feed conversion ratio (FCR). Birds fed diet with 2,400 kcal/kg ME had significantly lower BWG (P < 0.004), lower shank length (P < 0.0007), higher feed intake (P < 0.0001) and poor FCR (P < 0.0001) than those fed diet with either 2,600 or 2,800 kcal/kg ME. Energy efficiency ratio was not influenced by ME, CP or their interaction. However, protein efficiency ratio was significantly higher at higher ME levels and lower at higher CP levels. There was significant effect of ME, CP and their interaction on serum protein and cholesterol levels. However, they made no significant effect on antibody titre against New Castle disease vaccine. The study concluded that provision of 2,600 kcal/kg ME and 16% CP would be ideal for optimum growth of Aseel birds during juvenile phase. However, to obtain better FCR, feeding Aseel birds with diet having 2,800 kcal/kg ME and 16% CP would be ideal.
    Tropical Animal Health and Production 03/2012; 44(7):1653-8. · 1.09 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A study was designed to explore genetic variability of partial promoter and exon 1 of pit-1 gene and its effect on immune competence traits in White Leghorn chicken. Single stranded conformation polymorphism technique followed by sequencing was employed to detect polymorphism of the gene. Three genotypes (AA, AB and BB) were observed in White Leghorn chicken where two alleles (A and B) were found. AB genotype and A allele were predominant in the population. Different demographic parameters estimated in the White Leghorn chicken lines indicated the presence of genetic divergence among the lines. The pit-1 mRNA expression varied among the genotypes where genotype BB expressed higher than genotype AA and AB. The genotypes as well as alleles showed significant effect (P < 0.05) on sheep RBC titre in White Leghorn chicken.
    Journal of Applied Animal Research 12/2011; 39(4):298-302. · 0.12 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Single strand conformation polymorphism analysis and DNA sequencing was performed in White Leghorn hens to explore the polymorphisms present in the promoter of the prolactin gene. The effects of different genotypes on egg production and quality traits were determined, and expression of the prolactin gene in different genotypes was quantified by real time-PCR. Five genotypes and four alleles at each of two Fragments of the promoter were found, of which the FG genotype in Fragment 1 and the PQ genotype in Fragment 2 were the most predominant genotypes. The genotypes of Fragment 1 had significant effects (P < 0·05) on Haugh unit, albumen weight, albumen percentage and shell percentage at 40 weeks of age; egg weight and yolk index at 52 weeks of age; and egg weight at 64 weeks of age. Prolactin expression in the genotypes of Fragment 1 differed significantly and GH genotyped birds had the highest level of expression. The genotypes of Fragment 2 did not show any significant differences of expression. It was concluded that the prolactin gene promoter was highly polymorphic, and had significant effects on egg quality traits in White Leghorn hens.
    British Poultry Science 10/2011; 52(5):551-7. · 1.15 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Expression of prolactin hormone is a crucial event in regulating egg production in chickens for which promoter plays the vital role in expressing the prolactin gene. The objective of the present study was to identify haplotypes in the prolactin promoter and their effects on egg production and egg quality traits in White Leghorn chicken. Single stranded conformation polymorphism followed by sequencing was conducted to explore polymorphism at 561 bp promoter of prolactin gene. The effect of haplotype combinations on egg production and quality traits were estimated following general linear model technique. The expression of prolactin by different haplogroups was quantified by qPCR. Total 28 haplotypes were found in White Leghorn chicken of which h1 haplotype possessed the highest frequency of 0.46 and h8, h14, h16, h25, h26, and h28 haplotypes had the lowest frequency (0.1%). The egg production up to 52 and 64 weeks of age were found to be significantly (p < 0.05) associated with haplotype combinations where the highest 52-w (52 weeks) egg production was found in animals with h1/h22 combination and the lowest production was observed in the birds with h1/h2 haplogroup. The haplotype combinations had the significant effect (p < 0.05) on Haugh Unit, yolk index and albumen weight at 40 weeks of age; Haugh Unit and albumen weight at 52 weeks of age and Haugh unit, yolk weight and yolk percentage at 64 weeks of age. The prolactin expression in h1/h22 birds was found to be the lowest and in h1/h5 birds to be the highest. The prolactin expression showed significant effect on 52-w egg production and albumin weight at 52 weeks age. In conclusion, it may be stated that the prolactin promoter was highly polymorphic and had the significant association with egg production and quality traits in White Leghorn chicken.
    Animal Biotechnology 04/2011; 22(2):71-86. · 0.88 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A study was conducted to explore polymorphism at 5′ flanking region of prolactin gene and to estimate the effect of polymorphism on egg quality traits in naked neck chickens. Single-stranded conformation polymorphism technique was employed to detect polymorphism in the gene. Three genotypes (AA, AB and AC) were observed in naked neck chicken of which the predominant genotype was AB. The predominant and least frequent alleles in the population were A and C, respectively. The sequence alignment study revealed a number of nucleotide substitutions at different positions of the alleles. Genotypes showed significant effect (P < 0.05) on Haugh unit and yolk index where birds with AB and AC genotypes were the best performers. In conclusion, it may be inferred that 5′ flanking region of prolactin gene was polymorphic with having significant effect on some egg quality traits in the naked neck chicken.
    Journal of Applied Animal Research 03/2011; 39(1):72-76. · 0.12 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The juvenile growth and carcass traits were evaluated in a 4 × 4 full diallel crossing involving four colored broiler chicken lines viz., Naked neck (NN), Dwarf, Punjab Broiler-1 (PB-1), and Punjab Broiler-2 to study the performance and crossbreeding parameters. The data on 2,280 chicks were analyzed using least squares techniques to assess the effect of genetic group, and the significant traits were further analyzed for crossbreeding parameters. Genetic group had significant influence on the body weights and carcass traits. The cross of PB-1 × NN recorded significantly (p ≤ 0.05) higher body weight at 6 weeks of age. The data revealed that general combining ability (GCA), specific combining ability (SCA), maternal ability (MA), sex-linked effect (SE), and purebred effects significantly influenced the growth and carcass traits in the broiler crosses. The crossbred PB-1 × NN showed positive heterosis for growth as well as carcass traits. The results suggest that GCA, SCA, SE, and MA are important in the inheritance of the body weights and carcass traits indicating the presence of both additive and nonadditive genetic variation along with reciprocal effects. Therefore, pure line selection along with development of specialized sire and dam lines followed by crossing will improve the growth performance and benefit the poultry farmers. It is concluded that PB-1 as a male line and NN as a female line performed significantly higher, therefore, may be used for improving the performance of colored broilers.
    Tropical Animal Health and Production 03/2011; 43(6):1129-36. · 1.09 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The objective was to characterize polymorphisms at the 5'-UTR region of the prolactin gene, and determine their association with egg production and egg quality traits in White Leghorn chickens. The study was conducted on four strains of White Leghorn chickens, namely IWH, IWI, IWK, and layer control. Overall, there were three alleles (designated A, B, and C) and five genotypes, with genotypic frequencies of 0.09, 0.75, 0.07, 0.02, and 0.07 for AA, AB, AC, BB, and BC, respectively. There were significant differences among genotypes for egg production up to 52 and 64 wk of age, with maximal egg yields for genotypes AA and AC (144.5 ± 5.06 and 143.2 ± 4.67 eggs, respectively). Furthermore, there were significant differences among genotypes for egg quality traits, including egg weight and Haugh unit at 40 wk of age, Haugh unit at 52 wk, and yolk color index and Haugh unit at 64 wk. Birds with AA or AC genotypes had the best egg quality traits. On the contrary, these genotypes had the lowest prolactin expression, whereas this expression was highest in birds with the BB genotype. In conclusion, polymorphisms at the 5'-UTR of prolactin gene were significantly associated with egg production and egg quality traits in White Leghorn chickens.
    Theriogenology 03/2011; 75(4):655-61. · 2.08 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The pituitary hormone prolactin has a wide variety of functions involving growth, behavioral, and ovarian activities in chickens. The objectives of the present study were to identify polymorphisms in the prolactin promoter and estimate their effects on growth traits in White Leghorn chickens. Among 28 haplotypes found, the h1 haplotype was predominant. Body weight at 16 and 64 weeks and age at sexual maturity were significantly associated with haplotype combinations (P < 0.05). The h1/h1 haplogroup showed the highest body weight at 16 weeks of age, and h1/h7 was the highest at 64 weeks. The lowest age at sexual maturity was found in birds with the h1/h6 haplotype combination, and mRNA expression of prolactin was lowest in h1/h4 birds and highest in h1/h5 birds. The prolactin promoter was highly polymorphic and had significant associations with growth traits in White Leghorn chickens.
    Biochemical Genetics 01/2011; 49(5-6):385-94. · 0.94 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In the present experiment, the expression profile of Toll-like receptor mRNA in indigenous and pure line chickens was studied. The expression of TLR3, TLR4, TLR5 and TLR7 were quantified in heterophils of Aseel, Kadaknath, Naked neck, Dwarf and White Leghorn lines by Quantitative Real-time PCR. White Leghorns expressed significantly (P < 0.01) higher levels of TLR3 mRNA compared to other lines. TLR4 and TLR5 mRNA were significantly highly expressed in Kadaknath line. Among the TLRs investigated TLR5 was more expressed in all lines studied. TLR7 was highly expressed in indigenous chicken Aseel and Kadaknath than other lines. Dwarf chicken expressed significantly (P < 0.01) lower levels of all TLRs investigated. On the basis of the present study we conclude that the differential expression of TLR mRNA in the heterophils of indigenous and other chicken breeds might contribute to their variable disease resistance/susceptibility.
    Veterinary Research Communications 10/2010; 34(7):633-9. · 1.08 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To measure genetic relatedness between populations, for breeding purposes, we analyzed 170 birds from six crossbred populations of three pure lines of White Leghorn chickens, using 14 microsatellite markers. All the microsatellites were polymorphic, with 2-6 alleles. The mean number of alleles per locus was 3.21. The effective number of alleles varied from 1.14 to 3.94. The observed heterozygosity varied from 0.133 to 1.00, with a mean of 0.748. The F (IS) values were mostly negative, with an average of -0.345. The mean F (ST) value was 0.056. The Nm values ranged from 1.91 to 42.17. The highest genetic identity was observed between IWI x IWK and IWK x IWI. The relation between any two groups of crosses was more than 85%. The results suggest that the crossbred populations were very closely related.
    Biochemical Genetics 10/2010; 48(9-10):727-36. · 0.94 Impact Factor
  • Source
    Journal of Genetics 08/2010; 89(2):e33-7. · 0.88 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A comprehensive study was undertaken to evaluate the effect of naked neck (Na) gene on immune competence, serum biochemical parameters and carcass quality traits in three genotypes (NaNa, Nana and nana) of the naked neck chicken under a tropical climate (Southern India). Sixty day-old chicks (20 from each genotype) were selected randomly and reared under similar environmental conditions up to eight weeks of age. The cell mediated immune (CMI) response to phytohaemoagglutinin-P (PHA-P) was significantly higher () in NaNa and Nana genotypes compared to nana birds. The humoral response as measured by antibody titre to sheep red blood cells (SRBC) was also significantly higher in NaNa. The total cholesterol, LDL and VLDL cholesterol levels were significantly () lower whereas HDL cholesterol level was significantly higher in NaNa and Nana compared to nana genotype. The presence of Na allele significantly increased the live weight and dressing yield, and decreased the feather cover and abdominal fat. The naked neck genotypes (NaNa/Nana) performed better than the normal (nana) siblings for almost all the traits studied.
    Asian Australasian Journal of Animal Sciences 01/2010; 23(7). · 0.64 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A comprehensive study was undertaken to evaluate the egg quality traits in three naked neck genotypes, NaNa (homozygous), Nana (heterozygous) and nana (normal) by utilizing 556 eggs collected at 28, 32, 36 and 40 weeks of age. The overall mean egg weight, shape index and Haugh unit values were 56.41 g, 75.79 and 75.98, respectively. The yolk index, yolk height and yolk colour varied significantly (p < 0.01) in three genotypes. All other external and internal egg quality traits were not differed significantly among the genotypes; however Naked neck had better egg quality in almost all the traits. Age had significant (p < 0.01) effect on all the traits studied in Naked neck chicken. Haugh unit score was better in 28 week age group. The egg, yolk, albumin and shell weights were positively correlated with the age. The average shell weight and thickness values were 5.07 g and 0.401 mm, respectively, which had no significant variation among the genotypes. The Na gene had marginal effect on all the egg quality traits studied. The better egg quality parameters in naked neck chicken are desirable which indicate the better keeping quality of the eggs and withstand the handling damages.
    International Journal of Poultry Science. 01/2009;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study was conducted on six crossbred chicken populations of White Leghorn to estimate variability of microsatellites and their association with egg production traits. Five microsatellite markers located on chromosome 1, 2, 5 and 30 were explored and the association study was performed employing least square-maximum likelihood method. All the microsatellites were found to be polymorphic showing three to six alleles in the population. Genotype and allelic frequency was estimated showing a large variability in different microsatellites. The association study of microsatellite variability with egg production traits showed that only ADL023 microsatellite was significantly associated with egg production upto 64 and 72 weeks and egg weight at 28 weeks of age. Genotype 11, 12, 13 and 23 produced more number of eggs at 64 and 72 weeks of age than the genotype 22. Egg weight was higher in genotype 12, 13 and 23 and lower with genotype 11 and 22.
    International Journal of Poultry Science. 01/2008;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The growth and production performance of 4 chicken varieties developed for backyard farming were evaluated in the present study. The body weights were significantly (p<0.05) varied in 4 chicken varieties. The body weights were significantly higher in C<SUB>1</SUB> and Vanaraja and lower in Gramapriya. The C<SUB>2</SUB> and C<SUB>1</SUB> crosses matured early at the age of 145.72±1.11 and 154.67±0.81 days, respectively. The egg weights were significantly (p<0.05) higher in C<SUB>1</SUB> cross throughout the laying period. The egg weights ranged from 57.06±0.27 g (Vanaraja) to 58.58±0.29 g (C<SUB>1</SUB> cross) and 60.07±0.28 g (Vanaraja) to 61.34±0.35 g (C<SUB>1</SUB> cross) at 40 and 64 weeks of age, respectively. The egg production in C<SUB>1</SUB> cross was significantly (p<0.05) better than Vanaraja and C<SUB>2</SUB> cross at all ages, however the egg production was on par with Gramapriya at 64 and 72 weeks of age. C<SUB>1</SUB> cross has substantial production capabilities as dual purpose bird suitable for rural and backyard farming in the country.
    International Journal of Poultry Science. 01/2008;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The data of G-5 generation of inbred and non-inbred populations of Dahlem Red were utilized in the present study. These populations were originally developed by a regular system of full sib and half sib mating for 5 successive generations. A random mated non-inbred population was also maintained. Consequently, 3 sub populations have been developed from the same base population, namely, a full sib mated group (FS), a half sib mated group (HS) and a non-inbred group (NB). The inbreeding coefficient in the G-5 generation in the FS group and HS groups was 67.2% and 44.9%, respectively. These three sub populations were subjected to PCR-RAPD analysis to observe the genetic distance and similarity between different subpopulations. A total of 41 random primers (decamers) were screened for RAPD analysis, out of which 21 primers amplified the genomic DNA, generated 128 to 4544 bp bands. The Mean Average Percentage Difference (MAPD) varied between 13.22 and 22.20. The MAPD was highest between FS and NB and lowest between HS and FS. The intra-population genetic similarity was highest in FS (0.7139) followed by HS (0.7095) and NB (0.7054) which indicated that FS subpopulation is most uniform. The FS and NB had maximum genetic distance (by both band sharing and band frequency) and FS and HS had lowest genetic distance. The immune response to SRBC was highest in NB group followed by FS and HS groups.
    International Journal of Poultry Science. 01/2007;