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ABSTRACT: We describe an outbreak of 5 osteoarticular infections among 24 daycare center attendees. PCR revealed Kingella kingae in the joint fluid of one child and in 85% of throat samples from healthy contacts. MLST performed on the joint fluid and carriage isolates identified an unique sequence type. Rifampin failed to eradicate K. kingae carriage.
The Pediatric Infectious Disease Journal 01/2013; · 3.58 Impact Factor
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ABSTRACT: Background. Despite the increasing recognition of Kingella kingae as an important pathogen of early childhood, the relative frequency and invasiveness of different strains of the organism has not been investigated. A study was conducted to determine the association of K. kingae genotypes with specific clinical syndromes and the temporal and geographic distribution of invasive clones. Methods. A collection of 181 invasive K. kingae strains, isolated between 1991 and 2012 from Israeli patients with bacteremia, skeletal system infections, or endocarditis, were typed by pulsed-field gel electrophoresis (PFGE). In addition, the correspondence between PFGE, multilocus sequence types (MLSTs), and rtxA gene sequencing results was also examined for organisms belonging to the predominant PFGE clones isolated from asymptomatic carriers and patients with invasive infections. Results. A total of 32 different K. kingae clones were identified by PFGE, of which 5 (B, H, K, N, and P) caused 72.9% of all invasive infections, and were recovered during the 21-year period from different regions of the country. Clone K was significantly associated with bacteremia, clone N with skeletal system infections, and clone P with bacterial endocarditis. Strains belonging to the same PFGE clone, either carried asymptomatically or causing different invasive infections, shared MLST complexes and exhibited identical or closely related rtxA alleles. Conclusions. Although K. kingae exhibits noteworthy genetic heterogeneity, a limited number of distinct clones cause the majority of invasive infections in Israel, exhibiting genetic stability, long-term persistence, and wide geographic dispersal. K. kingae strains also show significant association with specific clinical syndromes.
Clinical Infectious Diseases 07/2012; 55(8):1074-9. · 9.15 Impact Factor
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Romain Basmaci,
Pablo Yagupsky,
Brice Ilharreborde,
Kathleen Guyot,
Nurith Porat,
Marilyn Chomton,
Jean-Michel Thiberge,
Keyvan Mazda,
Edouard Bingen,
Stéphane Bonacorsi,
Philippe Bidet
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ABSTRACT: Kingella kingae, a normal component of the upper respiratory flora, is being increasingly recognized as an important invasive pathogen in young children. Genetic diversity of this species has not been studied.
We analyzed 103 strains from different countries and clinical origins by a new multilocus sequence-typing (MLST) schema. Putative virulence gene rtxA, encoding an RTX toxin, was also sequenced, and experimental virulence of representative strains was assessed in a juvenile-rat model.
Thirty-six sequence-types (ST) and nine ST-complexes (STc) were detected. The main STc 6, 14 and 23 comprised 23, 17 and 20 strains respectively, and were internationally distributed. rtxA sequencing results were mostly congruent with MLST, and showed horizontal transfer events. Of interest, all members of the distantly related ST-6 (n = 22) and ST-5 (n = 4) harboured a 33 bp duplication or triplication in their rtxA sequence, suggesting that this genetic trait arose through selective advantage. The animal model revealed significant differences in virulence among strains of the species.
MLST analysis reveals international spread of ST-complexes and will help to decipher acquisition and evolution of virulence traits and diversity of pathogenicity among K. kingae strains, for which an experimental animal model is now available.
PLoS ONE 01/2012; 7(5):e38078. · 4.09 Impact Factor
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The Pediatric Infectious Disease Journal 12/2011; 30(12):1120-1; author reply 1121-2. · 3.58 Impact Factor
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Romain Basmaci,
Patricia Mariani,
Géraldine Delacroix,
Sonia Azib,
Albert Faye,
Muhamed-Kheir Taha,
Edouard Bingen,
Stéphane Bonacorsi,
José R Romero,
Harley A Rotbart,
Ann-Christine Nyquist,
Frederick S Nolte
Journal of clinical microbiology 09/2011; 49(9):3442-3. · 4.16 Impact Factor
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ABSTRACT: We conducted a retrospective study comparing the presenting clinical and biologic features of 64 children who had septic arthritis caused by Kingella kingae with 26 children who had septic arthritis caused by Staphylococcus aureus. Children with K. kingae septic arthritis were significantly younger than those with S. aureus septic arthritis. Otherwise, there were no significant differences between the 2 groups with respect to fever, location, white blood cell count, synovial fluid cell count, C-reactive protein, or serum fibrinogen. However, the clinical course was significantly better for children with septic arthritis caused by K. kingae as evidenced by shorter hospitalization and fewer adverse events. Presumptive antibiotic therapy for septic arthritis in young infants should take into account both of these pathogens, even in case of mild presentation.
The Pediatric Infectious Disease Journal 05/2011; 30(10):902-4. · 3.58 Impact Factor
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The Pediatric Infectious Disease Journal 09/2010; 29(9):890-1. · 3.58 Impact Factor
