[show abstract][hide abstract] ABSTRACT: The factors contributing to the oxidant/antioxidant imbalance in asthma are incompletely understood.
To determine the factors associated with oxidative stress including asthma severity and the genotype of the antioxidant enzymes.
A total of 196 children with mild asthma, 116 children with moderate-severe asthma, and 2 healthy control groups (187 and 68 children) were included in the study. Plasma levels of malondialdehyde were measured as the indicator of oxidative stress, and reduced glutathione levels as the indicator of antioxidant defense. Children were genotyped for null variants of glutathione S transferase (GST) T1 and GSTM1, and ile105val variant of GSTP1. Risk factors were analyzed with multivariate logistic regression.
Systemic levels of malondialdehyde increased and reduced glutathione levels decreased significantly from healthy controls to patients with mild asthma and then to patients with moderate-severe asthma (P < .001 for each). Multivariate logistic regression identified asthma and asthma severity as independent factors associated with oxidative stress (odds ratio between 17 and 56; P < .001). Children with asthma with GSTP1 val/val genotype had higher malondialdehyde and lower glutathione levels compared with other genotypes (P = .023 and P = .014, respectively). GSTP1 val/val genotype was independently associated with asthma severity (odds ratio, 4.210; 95% CI, 1.581-11.214; P = .004).
Our study indicates the presence of a strong oxidative stress in children with asthma that increases with the severity of the disease. In this population, val/val genotype at GSTP1 ile105val locus may be an important factor in determining the degree of oxidant injury.
Children with asthma with val/val genotype at GSTP1 ile105val locus may be good candidates for supplemental antioxidant therapy.
Journal of Allergy and Clinical Immunology 12/2006; 118(5):1097-104. · 12.05 Impact Factor
[show abstract][hide abstract] ABSTRACT: Our aims were to analyze the changes in hemoglobin (Hb) value according to gestational age, birth weight, sex, birth season, and weight gain and to detect distribution of Hb values among healthy infants, breastfed for at least four months and receiving routine health care. We conducted a descriptive study using the data of 469 healthy infants at 5-7 months of age in Hacettepe University Ihsan Doğramaci Children's Hospital Well-Baby Clinic between 2001-2004. Infants with acute or chronic illness, exchange transfusion and those who had taken or were currently taking iron supplementation were not included into the study. Information regarding the child was obtained from hospital files. Infants with Hb value <10.5 g/dl and <9.5 g/dl were considered to have mild and moderate anemia, respectively. The mean Hb value was 10.7 g/dl (SD = 0.90). The prevalence of anemia was 41.4%. Boys had significantly lower Hb, hematocrit and mean corpuscular volume values and higher red cell distribution width values than girls. Infants born before 37 weeks of gestational age had moderate anemia more frequently. Anemia at 5-7 months of age was more frequent in infants born in spring and summer than in those born in fall and winter (49.2%, 26.8%, p<0.001). Birth weight was positively correlated with Hb value at 5-7 months of age (r=0.14, p=0.003). In the present study, female gender, at-term birth, birth in winter and fall, weight appropriate for gestational age, and regular weight gain showed the lowest risk for anemia development in infants aged 5-7 months with a breast-feeding period of more than four months.
The Turkish journal of pediatrics 51(6):572-7. · 0.56 Impact Factor