Ravi Shankar

Publications (2)4.07 Total impact

• Source

  Available from: Heikham Russiachand Singh

  Article: De novo sequencing and characterization of Picrorhiza kurrooa transcriptome at two temperatures showed major transcriptome adjustments.

  Parul Gahlan, Heikham Russiachand Singh, Ravi Shankar, Niharika Sharma, Anita Kumari, Vandna Chawla, Paramvir Singh Ahuja, Sanjay Kumar
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  ABSTRACT: Picrorhiza kurrooa Royle ex Benth. is an endangered plant species of medicinal importance. The medicinal property is attributed to monoterpenoids picroside I and II, which are modulated by temperature. The transcriptome information of this species is limited with the availability of few hundreds of expressed sequence tags (ESTs) in the public databases. In order to gain insight into temperature mediated molecular changes, high throughput de novo transcriptome sequencing and analyses were carried out at 15 °C and 25 °C, the temperatures known to modulate picrosides content. Using paired-end (PE) Illumina sequencing technology, a total of 20,593,412 and 44,229,272 PE reads were obtained after quality filtering for 15 °C and 25 °C, respectively. Available (e.g., De-Bruijn/Eulerian graph) and in-house developed bioinformatics tools were used for assembly and annotation of transcriptome. A total of 74,336 assembled transcript sequences were obtained, with an average coverage of 76.6 and average length of 439.5. Guanine-cytosine (GC) content was observed to be 44.6%, while the transcriptome exhibited abundance of trinucleotide simple sequence repeat (SSR; 45.63%) markers.Large scale expression profiling through "read per exon kilobase per million (RPKM)", showed changes in several biological processes and metabolic pathways including cytochrome P450s (CYPs), UDP-glycosyltransferases (UGTs) and those associated with picrosides biosynthesis. RPKM data were validated by reverse transcriptase-polymerase chain reaction using a set of 19 genes, wherein 11 genes behaved in accordance with the two expression methods. Study generated transcriptome of P. kurrooa at two different temperatures. Large scale expression profiling through RPKM showed major transcriptome changes in response to temperature reflecting alterations in major biological processes and metabolic pathways, and provided insight of GC content and SSR markers. Analysis also identified putative CYPs and UGTs that could help in discovering the hitherto unknown genes associated with picrosides biosynthesis.
  BMC Genomics 03/2012; 13:126. · 4.07 Impact Factor
• Article: The market for wireless electricity: The case of India

  Ashish Kumar, Ravi Shankar, Kiran Momaya, Sandeep Gupte
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  ABSTRACT: A wireless revolution has transformed telecoms in India and in other emerging markets. The electricity market, on the other hand, remains underdeveloped. We define Wireless Electricity as renewable energy produced within a few hundred meters of the point of consumption. A wireless revolution in electricity would solve the problem of electricity deficit, empower people at the bottom of the pyramid and mitigate the environmental impact of bringing hundreds of millions out of poverty as the Indian economy grows. Renewables are technically proven and economically viable in certain situations, but their use remains peripheral. The stark difference in the diffusion patterns in telecoms and electricity has been ignored by leaders in government, business and academics. We present common frameworks to explain the different directions of reform in telecoms and electricity. We explain some of the dynamics which prevent the diffusion of Wireless Electricity. We use a causal loop diagram to explain the status quo in the off-grid electricity market and propose changes which will lead to the formation of a market for Wireless Electricity. India has the entrepreneurial talent to develop this market—and the largest number of potential customers. The world will benefit as a result.
  Energy Policy. 01/2010;