Raktim Mukherjee

The Maharaja Sayajirao University of Baroda, Vadodara, State of Gujarat, India

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Publications (7)5.12 Total impact

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    Cardiovascular toxicology 11/2010; · 2.56 Impact Factor
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    ABSTRACT: Present study evaluates cardioprotective role of melatonin (Mel), alpha lipoic acid (ALA), a combination of melatonin and alpha lipoic acid (Mel + ALA) against cadmium (Cd)-induced oxidative damage. Female albino rats were subjected to 15-day exposure to Cd (5.12 mg/kg bw) alone or treated with ML (10 mg/kg bw) + ALA (25 mg/kg bw) simultaneously. Plasma markers of cardiac damage, cardiac free radical generation, lipid peroxidation, endogenous antioxidant status, cadmium load, metallothionein induction, and histopathology were evaluated in various experimental groups. Combination of Mel + ALA significantly prevented leakage of marker enzymes of cardiac damage, changes in cardiac free radical generation, endogenous antioxidants, antioxidant status, structural alterations and augmented the degree of metallothionein (MT) induction. The results demonstrate that ML + ALA co-administration effectively protected against Cd-induced cardiac oxidative damage.
    Cardiovascular toxicology 11/2010; 11(1):78-88. · 2.56 Impact Factor
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    ABSTRACT: In the present study, a multi-dimensional therapeutic approach for combating experimentally induced Type I diabetes using polyherbal therapy with melatonin supplementation and an exercise regimen (S+M+PE) is employed in both diabetic (DC) and non diabetic (NC) animals. Adult albino rats were made diabetic by a single intraperitoneal injection of alloxan (120 mg / kg body weight). After a duration of 7 days, animals with a blood glucose level of 300 mg / dl or higher were considered diabetic. Control and experimental animals were subjected to swimming exercise together with administration of melatonin and PE for a further duration of 15 days and, upon sacrifice, various parameters related to glycaemic status and carbohydrate metabolism were evaluated. Results revealed a much better glycaemic regulation and carbohydrate metabolism. The glucose clearance rates of DC.S+M+PE animals under both glucose challenge and exogenous insulin were much greater than both NC and DC animals. Observed increase in insulin levels in both NC and DC animals subjected to S+M+PE was an indicative of observed near normal glucoregulation with combination therapy. S+M+PE exerted favourable influence on glucose uptake and utilization by way of increased insulin titre and sensitivity as well as up regulation of GLUT-4. In conclusion it can be said that S+M+PE is an effective combination therapy exerting effective glucoregulation and correction of carbohydrate metabolism.
    Int J Biol Med Res. 01/2010; 1:54 - 60.
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    ABSTRACT: The present study is a multi-dimensional approach aimed at assessing the efficacy of a polyherbal extract in combination with melatonin intake, as a safe natural anti-oxidant of the body, along with a regimen of swimming exercise. Adult albino rats were made diabetic by a single intraperitoneal injection of alloxan (120 mg / kg body weight). Control and experimental animals were subjected to swimming exercise together with administration of melatonin and PE for a further duration of 15 days and, upon sacrifice, various parameters related to lipid metabolism, enzymic and non enzymic markers of oxidative stress and serum markers of hepatic and renal dysfunction were evaluated. Results revealed a differential response in the form of lowered tissue lipid and cholesterol load though with certain degree of renal oxidative stress and abnormal renal function and a marginally higher serum levels of cholesterol and lipid. In contrast to non-diabetic animals, diabetic ones were more sensitive to S+M+PE treatment schedule as marked by the significant depletion in tissue lipid and cholesterol contents as well as anti hypertriglyceridemic and hypercholesterolemic effects. The present study involving a combination therapy seems to cause some oxidative stress as marked by significant decrement in CAT and SOD activities of liver, muscle and kidney along with GSH depletion with no increase in LPO. Increase in serum Corticosterone level could be speculated to be contributor of oxidative stress. In conclusion it can be said that S+M+PE is an effective combination therapy exerting antilipidemic effects but with some incompetence in containing sub adaptive swimming exercise induced oxidative stress and hepatic and renal dysfunctions. A properly worked out intensity and duration dependent adaptive exercise schedule is likely to overcome these shortcomings. Such an exercise schedule needs to be then studied in conjunction with melatonin supplementation and PE treatment.
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    ABSTRACT: Ayurvedic remedies for diabetes are usually mixed formulations containing blood sugar lowering herbs in combination with immunomodulators, diuretics and detoxicants, with the rationale behind such formulations being provided by modern research. The plants for the present study were selected on the basis of their anti-diabetic property, as these plants are consumed by the local people for treatment of diabetes as also supported by literature survey. Plants like Annona squamosa(Annonaceae), Cassia fistula, Coccinia indica, Mangifera indica, Ocimum sanctum, Lagerstroemia losflos-reginee, and Murraya koenigii used in the present study have been known to have same blood glucose lowering effect in diabetic animals and, some of them have an anti-lipidemic and cholesterol lowering effects too. To this effect, adult albino rats were made diabetic (DC) by a single i.p injection of alloxan (120 mg / kg body weight). Animals having blood glucose level of 300 mg / dl or higher were considered diabetic. Control and experimental animals were supplemented with the extract (PE) subjected to swimming exercise(s) of 30 minutes duration for 15 days. Animals were sacrificed on the 16 th day and various parameters related to glycaemic status and carbohydrate metabolism were evaluated. The results of the present study showed significant favorable effect of S+PE in non-diabetic as well as diabetic rats. In terms of glycaemic regulation, neither PE nor PE+S showed any significant effect either on fasting or fed glycaemic level in non-diabetic animals while, both were equally effective in minimizing diabetic hyperglycaemia to the same extent. In DC animals, both PE and S+PE improved the insulin status. There was increased hepatic and muscle glycogen contents in both NC and DC animals treated with PE or also subjected to exercise. The increased tissue glycogen load and glucose clearance from blood are paralleled by significant decrement in tissue glycogen phosphorylase activity. There was decrement in cytosolic GLUT 4 level in NC.PE and NC.S+PE animals and significant increment in expression in DC.PE and DC.S+PE animals over that of the low level in DC animals. Overall, the present study provides support and evidence for consideration of a therapeutic approach combining the beneficial effects of a polyherbal preparation in association with adaptive physical activity for effective management of diabetic complications.
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    ABSTRACT: Dietary exposure to cadmium, even at lower doses, can lead to free radical induced neurotoxicity, neurobehavioral changes and alteration in neurotransmitters. Such changes are likely to be more pronounced in the developing brain due to incompleteness of blood brain barrier (BBB). Hippocampus being the seat of intelligence has a role in learning and cognitive behavior and any damage to hippocampus during developmental stage is likely to result in neurodegenerative changes in later life. To this end, fetal and neonatal exposure to cadmium was induced by exposing pregnant dams of Swiss albino strain throughout the period of gestation and following parturition up till 5th day post partum (pp) through drinking water (3ppm/animal/day). The neonates were sacrificed on day 6 pp and indices of oxidative stress, levels of trace elements and changes in cholinergic system were evaluated in the hippocampus. Increased lipid peroxidation, surge in reactive oxygen species (ROS), depressed antioxidant defense, increased accumulation of cadmium, differential alterations in trace elements and decreased activity of AChE were the features of cadmium toxicity. Simultaneous administration of melatonin to cadmium challenged animals offset these detrimental changes. The results suggest that melatonin co-administration can effectively protect against the adverse effects of cadmium on endogenous antioxidant status, changes in trace metal concentrations and compromised hippocampal cholinergic system.
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    ABSTRACT: Modification of dietary habits and life style changes such as, regular exercise together with mixed formulations containing blood sugar lowering herbs in combination with antioxidants and detoxicants, have been incorporated in the management of type -1 diabetes. Aqueous extract of 7 plants (Annona squamosa(Annonaceae), Cassia fistula, Coccinia indica, Mangifera indica, Ocimum sanctum, Lagerstroemia losflos-reginee, and Murraya koenigii) (PE) together with swimming exercise (S) has been employed in the present study to evaluate the efficacy against dyslipidemia and oxidative stress associated with type-1 diabetes. To this effect, adult albino rats were made diabetic (DC) by a single i.p injection of alloxan (120 mg / kg body weight). Animals having blood glucose level of 300 mg / dl or higher were considered diabetic. Control and experimental animals were supplemented with PE and subjected to swimming exercise of 30 minutes duration for 15 days. Animals were sacrificed on the 16 th day and, various parameters related to oxidative stress (SOD, CAT, GPx, GSH and LPO) and lipid metabolism (Total lipids, TG, TC, LDL, VDL, and VLDL) were evaluated. Diabetic animals showed down regulated antioxidant defence and dysregulation of lipid metabolism. Both PE and S+PE restored the level of antioxidants and markers of lipid metabolism to near normal levels. Overall, the present study provides support and evidence for consideration of a therapeutic approach combining the beneficial effects of a polyherbal preparation in association with adaptive physical activity for alleviating symptoms of tissue oxidative damage and disorders of lipid metabolism.

Publication Stats

16 Citations
5.12 Total Impact Points

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Institutions

  • 2010
    • The Maharaja Sayajirao University of Baroda
      • Department of Zoology
      Vadodara, State of Gujarat, India