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ABSTRACT: To study a glucan (GB II) isolated from Gastrodia elata. The glucan was obtained with water extraction, alcohol precipitate, DEAE-Sepharose Fast Flow column and Sepharose CL-6B column chromatography; sugar composition analysis, IR and NMR were used to determine the structural feature. The molecular weight of the glucan was 4 300 dalton estimated by HPGPC; it contained 27 glucose residues, which mainly composed of alpha-D-(1-->4)-glucose with little glucuronic acid and branch O-6 points. The glucan was a new glucan for the first report from Gastrodia elata.Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 05/2010; 33(5):726-9.