[show abstract][hide abstract] ABSTRACT: Large scale propagation of three mangrove species, Excoecaria agallocha, Heritiera fomes and Intsia bijuga using cuttings and air layering was attempted. The effect of auxins and season on rooting potential of these mangrove species was recorded. Maximum rooting was recorded when the cuttings and air layers were treated with IBA alone up to 2500 ppm in all the three species. October was found to be best followed by January for the plantation of cuttings and initiation of air layers. All the plants were hardened and field transferred into the mangrove forests of Pichavaram, Tamilnadu, India.
Wetlands Ecology and Management 01/2000; 8(1572-9834):281–286. · 1.08 Impact Factor
[show abstract][hide abstract] ABSTRACT: An in vitro propagation protocol has been developed for Excoecaria agallocha L. (Euphorbiaceae), a mangrove species. Nodal segments were used for axillary shoot proliferation. One shoot from each node of bimodal explants was observed 3 weeks after inoculation. The best axillary sprouting was seen on a newly formulated medium containing BA, Zeatin and IBA in concentrations of 13.3 mM, 4.65 mM and 1.23 mM, respectively. The new medium, first used in this study, has a specific composition of major nutrients, MS micronutrients and iron compounds. Nodal segments from rooted cuttings and seedlings responded better than those of mature tree explants. Multiple shoot induction was complemented with efficient shoot elongation, and repeated subculture of binodal segments from axillary shoots resulted in 10–12 shoots per explant in 3 months. Rooting was achieved by growing shoots in the new medium with 0.23 mM IBA. Regenerated plants were successfully acclimatized to the natural environment, and about 85% of plantlets survived under ex vitro conditions. This is the first report of micropropagation in the genus Excoecaria and also in mangrove tree species.
[show abstract][hide abstract] ABSTRACT: . Micropropagation of Porteresia coarctata Tateoka, a wild relative of rice with useful genetic traits of salinity and submergence tolerance, was achieved through nodal
segment culture. Woody Plant (WP) medium supplemented with benzyladenine (5.5 μm) and kinetin (2.3 μm) gave the greatest response to initiation and multiplication. The multiplication rate of 11 shoots/explant with an average
shoot length of 3.5 cm was observed after 8 weeks of culture period. The rooting response was observed simultaneously in the
multiplication media, but subsequent establishment was poor. When the in vitro raised shoots were transferred to optimal 1/2
WP and 1/2 MS media with 10.7 μmα-naphthaleneacetic acid, the rooting response was enhanced. Such rooted plants were hardened successfully under field conditions.