Ping Zou

Wuhan Union Hospital, Wu-han-shih, Hubei, China

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Publications (209)195.14 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: In an attempt to establish the advantages of fluorescence in situ hybridization (FISH) studies over conventional cytogenetic (CC) analysis, a total of 2302 de novo MDS patients from 31 Chinese institutions were prospectively selected in the present study for both CC and standardized FISH analysis for +8, -7/7q-, -5/5q-, 20q- and-Y chromosomal abnormalities. CC analysis was successful in 94.0% of the patients; of these patients, 35.9% of the cases were abnormal. FISH analysis was successful in all 2302 patients and detected at least one type of common cytogenetic abnormality in 42.7% of the cases. The incidences of +8, -7/7q-, -5/5q-, 20q- and-Y chromosomal abnormalities by FISH were 4.1% to 8.7% higher than those by CC. FISH identified abnormalities in 23.6% of the patients exhibiting normal CC results and revealed that 20.7% of the patients with adequate normal metaphases (≥20) had abnormal clones. FISH identified cytogenetic abnormalities in 50.4% of the patients with failed CC analysis. In summary, our multicenter studies emphasised and confirmed the importance of applying standardized FISH testing based on an appropriate panel of probes to detect common cytogenetic abnormalities in Chinese de novo MDS patients, particularly those with normal or failed CC results. Copyright © 2015. Published by Elsevier Ltd.
    Leukemia research 02/2015; 39(5). DOI:10.1016/j.leukres.2015.02.005 · 2.35 Impact Factor
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    ABSTRACT: Objective: To report the critical role of decitabine in treatment of a patient with T cell acute lymphoblastic leukemia (T-ALL) transformed from myelodysplastic syndrome (MDS) post-transplantation.Methods: This patient initially displayed MDS-refractory cytopenia with multilineage dysplasia (RCMD) with a chimeric chromosome of trisomy 8 and normal karyotype. Two years later, he had progressed to T-ALL, his karyotype had completely transformed to trisomy 8. Six months after a matched-related allogeneic peripheral blood stem cell transplantation (allo-PBSCT), he presented with a tumor measuring 5.5 × 3.9 × 7.1 cm in front of the mediastinum that was metabolically active (SUVmax 6.6), assessed using position emission tomography–CT (PET–CT). Tumor immunostaining was positive for CD3, TdT, CD99, CD4, CD8 and Ki67, suggestive of T cell lymphoblastic lymphoma. One month after immunosuppression had been discontinued, the tumor size decreased to 4.0 × 3.3 × 5.5 cm with a reduction in metabolic activity to 3.0 (SUVmax). Subsequently, he was treated with cyclosporine A and prednisone against chronic graft versus host disease. He was then treated with decitabine alone (20 mg/m2/d, 5 days/month) for 4 months without serious side effects.Results: Thus far, his bone marrow has remained in complete remission. PET–CT scan revealed a decrease in tumor size to 2.6 × 1.9 × 3.3 cm and a reduction in metabolic activity to 1.5 (SUVmax) without the detection of current disease during therapy.Conclusions: Decitabine can be used in extramedullary relapse of T-ALL transformed from MDS following allo-PBSCT.
    Expert Opinion on Orphan Drugs 11/2014; 2(12). DOI:10.1517/21678707.2014.978285 · 0.53 Impact Factor
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    ABSTRACT: To investigate the efficacy and safety of rituximab together with etoposide, carboplatin, and ifosfamide (R-ICE) as a salvage therapy for relapsed diffuse large B-cell lymphoma (DLBCL) after treatment with rituximab based first-line chemotherapy (R-Chemo). DLBCL patients with complete remission (CR) or complete remission unconfirmed (CRu) after 6-8 cycles of R-Chemo treatment but relapsed for first time after stopping treatment were included in this study. Three cycles of R-ICE regimen were given to the patients [1st day: rituximab, 375 mg/m²; 2nd-4th day: ifosfamide, 1 600 mg/m²; 3rd day: carboplatin, area under the curve (AUC) =5 (maximum dosage: 800 mg), 2nd-4th day: etoposide, 100 mg/m²]. The primary endpoint was overall response rate (ORR). The secondary endpoints were the 2-year progression-free survival (PFS), 2-year overall survival (OS), and toxicity. Thirty-two patients with median age at 55(range: 26-68) were recruited in this clinical study and the final analysis. After three cycles of R-ICE salvage treatment, 16 patients (50.0%) achieved CR or CRu and 9 patients (28.1 %) achieved partial remission (PR). The ORR was 78.1%. The 2-year PFS and OS were 40.8% and 60.7%, respectively. Nineteen patients (59.4%) had 3/4 grade adverse events. The ratios of leukopenia neutropenia, anemia and thrombocytopenia in patients with 3/4 grades were 37.5%, 15.6%, and 37.5%, respectively. No patient died. R-ICE is an effective salvage therapy for R-Chemo relapsed DLBCL with manageable toxicities.
    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 04/2014; 35(4):314-7. DOI:10.3760/cma.j.issn.0253-2727.2014.04.014
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    ABSTRACT: The cytokines of acute leukemia (AL) patients have certain expression patterns, forming a complex network involved in diagnosis, progression, and prognosis. We collected the serum of different AL patients before and after complete remission (CR) for detection of cytokines by using an antibody chip. The expression patterns of cytokines were determined by using bioinformatics computational analysis. The results showed that there were significant differences in the cytokine expression patterns between AL patients and normal controls, as well as between acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). In confirmatory test, ELISA revealed the expression of uPAR in AL. Moreover, the bioinformatic analysis showed that the differentially expressed cytokines among the AL groups were involved in different biological behaviors and were closely related with the development of the disease. It was concluded that the cytokine expression pattern of AL patients is significantly different from that of healthy volunteers. Also, differences of cytokine expression patterns exist between AML and ALL, and between before and after CR in the same subtype of AL, which holds important clinical significance for revealing disease progression.
    Journal of Huazhong University of Science and Technology 04/2014; 34(2):176-80. DOI:10.1007/s11596-014-1254-8 · 0.83 Impact Factor
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    ABSTRACT: This study was aimed to investigate the effect of exogenous VEGF on hematopoietic stem cell mobilization and immune system. The C57BL/6J mice were randomly divided into the normal control group, VEGF short-term group (5 d) and VEGF long-term group (27 d). Mice in the experimental group were injected ip with VEGF (100 ng/d); mice in control group were injected ip with PBS. The white blood cell (WBC) count and the ratio of lymphocyte in the peripheral blood at different time point were assayed by hemacytometer. The percentage of hematopoietic stem cell (HSC), lymphocyte subgroup, regulatory T cell (Treg), myeloid-derived suppressor cells (MDSC) in the peripheral blood and spleen of different groups were detected by flow cytomertry. The morphological changes of spleen and spleen index of mice in the control and long-term group were observed by microscopy. The results showed that the absolute number of WBC in the peripheral blood of mice significantly increased after injection of VEGF, and the peak value was at day 3. The percentage of Lin(-)Sca-1(+)CD117(+) cells in the peripheral blood and spleen of the long-term group were significantly higher than that in the normal control group (P < 0.05). The spleen of the mice in VEGF long-term group was larger than that of the control group, the spleen index also increased (P < 0.05), and remarkable extramedullary hematopoietic signs were found in the HE stained sections. There was no significant change in the total ratio of lymphocytes in the peripheral blood after injection, but the percentage of CD3(+) cells and the CD3(+)/B220(+) ratio in the long-term group deceased; the percentages of Treg and Gr-1(+)CD11b(+) MDSC in the experimental groups increased (P < 0.05), which more significantly increased in the long-term group than that in the short-term group (P < 0.05). It is concluded that the exogenous VEGF promotes hematopoietic stem cell mobilization, and at same time up-regulates the many kinds of suppressive immune cell levels which leads to changes of immunofunction.
    Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology 03/2014; 22(1):154-9.
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    ABSTRACT: The purpose of this research was to establish an analytical method for analysing the 1-[6-chloro-3-methyl-pyridyl-8-nitro-7-methyl-1 2 3 5 6 7-hexahydro imidazo-(1,2a)]-pyridine (IPP) residue levels and to evaluate the difference in plant growth and its physical condition. A high performance liquid chromatography connected to a diode array detector (HPLC-DAD) was also employed. The results showed that the content of protein and water soluble carbohydrate (WSC) treated by IPP were initially higher with a significant delayed decrease. The biomarker response showed, even at a lower dose rate, exposure to the IPP caused stress effects and modified the activity of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT) and polyphenol oxidase (PPO). Different patterns of biomarker responses were observed by an increase in SOD and malondialdehyde (MDA), and differential effects for antioxidant enzymes with a decrease in CAT, POD and PPO. The conclusions show that this profile of biomarker variation could represent a useful method to characterise exposure to IPP in a wheat plant.
    Food Chemistry 03/2014; 146:569-76. DOI:10.1016/j.foodchem.2013.09.085 · 3.39 Impact Factor
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    ABSTRACT: This study was aimed to investigate a more convenient and efficient method to cultivate the human bone marrow mesenchymal stem cells by means of natural erythrocyte sedimentation principle, based on the whole bone marrow adherent method. The bone marrow was cultured with a six-well plate instead of the flasks.Firsly, the bone marrow specimen was cultivated with the MSC complete medium for 48 h, then the upper RBC-free supernatant layer was drawn and placed into the new wells to isolate MSC. Inverted microscope was used to observe the cell morphology and to record the adherent time of first cell passage, first passaging time. The traditional whole bone marrow adherent method was used as the control. The cell cycle and cell surface markers were detected by flow cytometry,and the differentiative capacity of MSC into osteocyte and adipocyte was identified by alkaline phosphatase kit and oil red O, respectively. Besides, the proliferative curve of P1,P3,P5 of BMSC was depicted by counting method. The results showed that MSC cultured by the modified method highly expressed CD90, CD105, CD13, CD44 and lowly expressed CD14, CD45, CD34. Concerning the cell cycle feature, it was found that most of the cells were in G0/G1 phase (88.76%) , followed by G2/M phase (3.04%) and S phase (8.2%), which was in accordance with stem cell cycle characteristics. The proliferative curve showed a typical "S" type, and both the oil red O and alkaline phosphatase staining of MSC were positive. Compared with the traditional method, the modified method had the advantage of high adherence rate (P = 0.0001) and shorter passaging time for the first passage (P = 0.001), with the statistically significant difference. It is concluded that there is a large number of adherent, active and suspended MSC in the RBC-free supernatant layer after the culture of bone marrow for 48 h. Isolating MSC by the modified method is more convenient and efficient than the traditional whole bone marrow adherent method.
    Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology 03/2014; 22(2):496-502.
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    ABSTRACT: To exam the role of leukemia cells-derived microparticles in the post-complete molecular response stratification. Blood samples from 29 patients diagnosed with chronic myeloid leukemia (CML) were collected. Microparticles (MP) were extracted from the peripheral blood. Real-time PCR was performed to measure the level of BCR-ABL mRNA. BCR-ABL mRNA could be stably detected both in MP and peripheral blood cells; BCR-ABL in MP showed significant difference within complete molecular response, major molecular response and complete cytogenetic response (9.1±2.8, 25.2±6.9 and 62.8±6.3 respectively, P<0.05). BCR-ABL was detected in MP even when it was negative in peripheral blood cells (3.7-15.3). For patients with complete molecular response, BCR-ABL in MP but not cells were significantly different between imatinib and stem cell transplant recipients (3.3±2.1 vs 9.1±2.8, P<0.05). This study indicated that MP may serves as a new target for monitoring of CML. Quantification of BCR-ABL in MP may offer a novel strategy for stratification of molecular response.
    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 02/2014; 35(2):138-141. DOI:10.3760/cma.j.issn.0253-2727.2014.02.017
  • X Zhu · Y You · Q Li · C Zeng · F Fu · A Guo · H Zhang · P Zou · Z Zhong · H Wang · Y Wu · F Kong · Z Chen
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    ABSTRACT: Malignant transformation of normal hematopoietic transplants induced by residual leukemia cells is considered as a pivotal mechanism of donor cell leukemia (DCL). The effects of leukemia cell-derived microvesicles (MVs) in this transformation were examined. We found that MVs derived from K562 leukemia cells contained the breakpoint cluster region-Abelson leukemia gene human homolog 1 (BCR-ABL1) mRNA. Following incubation with BCR-ABL1-positive MVs, mononuclear cells derived from normal transplants exhibited a leukemia-like malignant phenotype both in vitro and in vivo. Horizontal transfer of BCR-ABL1 mRNA from MVs into the recipient cells was critical to the transformation. Relative genomic instability was observed and considered the main mechanism in the recipient cells. MVs contributed to genomic instability by two distinct pathways: via consequent overexpression of activation-induced cytidine deaminase and reactive oxygen species, which mediated DNA breakage and recombination; and via upregulation of methyltransferases and global DNA hypermethylation. We demonstrated that BCR-ABL1-positive MVs could initiate malignant transformation of normal hematopoietic transplants through genomic instability, which might serve as a convenient and operable model for investigating leukemogenesis, especially for DCL. Furthermore, MVs themselves could act as an early warning indicator and a novel tool to detect and prevent the occurrence of DCL.Leukemia accepted article preview online, 31 January 2014. doi:10.1038/leu.2014.51.
    Leukemia: official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 01/2014; 28(8). DOI:10.1038/leu.2014.51 · 10.43 Impact Factor
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    ABSTRACT: Aim: To investigate the effects of serum deprivation (SD) on microvesicles (MVs) secreted from human myeloma cells and the implications for disease progression. Methods: RPMI 8226, U266, and KM3 human myeloma cells were incubated in medium containing 10% (non-SD) or 1% fetal bovine serum (SD) and MVs were isolated. The levels and size distribution of MVs were analyzed with flow cytometry. The protein profiles of MVs were studied using 2D SDS-PAGE, MALDI-TOF-MS, and Western blotting. NF-κB activation was analyzed using EMSA. Angiogenesis was examined in Eahy926 endothelial cells. Results: Exposure of RPMI 8226 cells to SD for 24 h did not alter the number of apoptotic cells. However, SD increased the number of MVs from RPMI 8226, U266, and KM3 cells to 2.5-, 4.3-, and 3.8-fold, respectively. The size distribution of SD MVs was also significantly different from that of non-SD MVs. Three proteins ZNF224, SARM, and COBL in SD MVs were found to be up-regulated, which were involved in cell cycle regulation, signal transduction and metabolism, respectively. Co-culture of SD MVs and RPMI 8226 cells increased NF-κB activation in the target RPMI 8226 cells. Furthermore, SD MVs from RPMI 8226 cells significantly increased the microtubule formation capacity of Eahy926 endothelial cells compared with non-SD MVs. Conclusion: SD elevates the levels of microvesicles with different size distribution and selectively enriched proteins in human myeloma cells in vitro. The selectively enriched proteins, especially ZNF224, may play key roles in regulation of myeloma cells, allowing better adaptation to SD.
    Acta Pharmacologica Sinica 12/2013; 35(3). DOI:10.1038/aps.2013.166 · 2.91 Impact Factor
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    ABSTRACT: The effects of granulocyte colony-stimulation-factor (G-CSF) on stem cell mobilization and its impact on the amplification of myeloid-derived suppressor cells (MDSCs) of donor mice were examined. A mouse model of stem cell mobilization was established by consecutive subcutaneous injection of 100 μg/kg G-CSF for 5 days. The blood from the donor mice was routinely examined during mobilization. Stem cells and MDSCs were analyzed by flow cytometry. The immunosuppressive molecules derived from MDSCs in serum and spleen, including hydrogen dioxide (H2O2) and nitric oxide (NO), and the activity of nitric oxide synthase (NOS) were determined during the mobilization. Apoptosis of T lymphocytes was assessed by using Annexin-V/PI. During stem cell mobilization, the number of lymphocytes and white blood cells in the peripheral blood was increased, and peaked on the 4th day. The number of stem cells in G-CSF-treated mice was significantly greater than that in controls (P<0.01). The expansions of MSDCs were also observed after G-CSF mobilization, with a more notable rate of growth in the peripheral blood than in the spleen. The activity of NOS and the production of NO were increased in the donor mice, and the serum H2O2 levels were approximately 4-fold greater than the controls. Consequently, apoptosis of T lymphocytes was increased and showed a positive correlation with the elevated percentage of MDSCs. It was concluded that G-CSF could provide sufficient peripheral blood stem cells for transplantation. Exogenous administration of G-CSF caused the accumulation of MDSCs in the peripheral blood and the spleen, which could lead to apoptosis of T lymphocytes and may offer a new strategy for the prevention and treatment of graft versus host disease.
    Journal of Huazhong University of Science and Technology 12/2013; 33(6):817-821. DOI:10.1007/s11596-013-1204-x · 0.83 Impact Factor
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    ABSTRACT: Effects of sodium acetate and glucose on the microbes from the simultaneous flue gas desulfurization and denitrogenation tandem biotrickling process were respectively investigated. For the acidophiles, although the impacts of the carbon sources on the total microbial growth and nitrogen conversion in the medium were slight, the sulfite oxidation rate was obviously improved. The highest sulfite ion oxidation ratio in 48 h was up to 47.2 % as 258.08 mmol L−1 sodium acetate was added in the medium. For the neutrophiles, the two carbons promoted the total microbial growth, but only sodium acetate could enhance the nitrite consumption significantly. The best nitrite consumption rate in 48 h could reach to 86.7 % with 258.08 mmol L−1 sodium acetate. It provides valuable information for the development of biological SO2 and NOx simultaneous purification process.
    Environmental Earth Sciences 11/2013; 70(5). DOI:10.1007/s12665-013-2452-6 · 1.77 Impact Factor
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    ABSTRACT: Objective: It was our aim to study the diagnostic significances of various dysplasia characteristics in myelodysplastic syndrome (MDS). Methods: We analyzed 160 cases of primary MDS and a control group including 28 cases of paroxysmal nocturnal hemoglobinuria (PNH), 104 cases of idiopathic thrombocytopenic purpura (ITP), 53 cases of non-severe aplastic anemia (NSAA), 40 cases of megaloblastic anemia and 50 cases of infectious and autoimmune diseases. Peripheral blood smears and bone marrow morphology were reviewed. Results: There was no significant difference in the occurrence rates of a variety of dysplasias in three lineages among MDS, megaloblastic anemia and PNH; however, changes in qualities and quantities in three lineages between NSAA and MDS were significantly different. ITP and MDS showed statistical differences in multiple changes in myeloid and erythroid cells. Significant differences also existed in multiple changes in erythroid series and megakaryocytes between infectious and autoimmune diseases and MDS. Morphological abnormalities highly related with MDS included multinucleated erythroblasts, ringed sideroblasts, poikilocytosis and gigantocytes, pseudo-Pelger neutrophils, ring-shaped nucleus, and micromegakaryocytes. Conclusions: It is difficult to discriminate megaloblastic anemia and PNH from MDS by means of cell morphology. Different dysplasias of MDS have specific diagnostic values.
    Acta Haematologica 10/2013; 131(2):126-132. DOI:10.1159/000351272 · 1.12 Impact Factor
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    ABSTRACT: The mammalian target of rapamycin (mTOR) is an important regulator of hematopoietic stem cell (HSC) self-renewal and its overactivation contributes to HSC premature exhaustion in part via induction of HSC senescence. Inhibition of mTOR with rapamycin has the potential to promote long-term hematopoiesis of ex vivo expanded HSCs to facilitate the clinical application of HSC transplantation for various hematologic diseases. A well-established ex vivo expansion system for mouse bone marrow HSCs was used to investigate whether inhibition of overactivated mTOR with rapamycin can promote long-term hematopoiesis of ex vivo expanded HSCs and to elucidate the mechanisms of action of rapamycin. HSC-enriched mouse bone marrow LSK cells exhibited a time-dependent activation of mTOR after ex vivo expansion in a serum-free medium supplemented with stem cell factor, thrombopoietin, and Flt3 ligand. The overactivation of mTOR was associated with induction of senescence but not apoptosis in LSK cells and a significant reduction in the ability of HSCs to produce long-term hematopoietic reconstitution. Inhibition of overactivated mTOR with rapamycin promoted ex vivo expansion and long-term hematopoietic reconstitution of HSCs. The increase in long-term hematopoiesis of expanded HSCs is likely attributable in part to rapamycin-mediated up-regulation of Bmi1 and down-regulation of p16, which prevent HSCs from undergoing senescence during ex vivo expansion. These findings suggest that mTOR plays an important role in the regulation of HSC self-renewal in vitro and inhibition of mTOR hyperactivation with rapamycin may represent a novel approach to promote ex vivo expansion and their long-term hematopoietic reconstitution of HSCs.
    Transplantation 10/2013; 97(1). DOI:10.1097/TP.0b013e3182a7fcf8 · 3.83 Impact Factor
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    ABSTRACT: The clinical characteristics of patients with seizures after allogeneic hematopoietic stem cell transplantation (allo-HSCT) were analyzed. A total of 8 cases of seizures after allo-HSCT were investigated. Clinical data of these cases were studied retrospectively. Of 159 cases subjected to allo-HSCT, seizure occurred in 8 cases during 29-760 days after transplantation, median survival time was 46 days, and there were 6 cases of tonic-clonic seizure. The incidence of seizure after matched unrelated HSCT was higher than that after related HSCT (P=0.017). Of 7 cases treated with cyclosporine A (CsA), 4 cases obtained high blood levels of CsA. In addition, hyponatremia was diagnosed in 5 cases. Abnormal electroencephalogram and brain MRI findings were found in some cases. During 20 days after seizure, 2 cases died due to infection and graft-versus-host disease (GVHD), respectively. It was suggested that multiple factors are associated with seizures after allo-HSCT. Rapid identification and correction of the causative factors are very important to prevent permanent central nervous system damage and reduce the mortality.
    Journal of Huazhong University of Science and Technology 10/2013; 33(5):656-60. DOI:10.1007/s11596-013-1176-x · 0.83 Impact Factor
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    ABSTRACT: To clarify the role of nucleostemin (NS) in AML, its transcription levels in bone marrow (BM) samples obtained from 128 newly diagnosed AML patients were analyzed. We determined that the highest NS transcription level was in M1 patients, while the lowest NS transcription level was in M3 patients. NS mRNA expression is positively correlated with blast percentages (%) and CD34, CD117 and CD123 antigen expression in BM samples but is unrelated to the transcription level of WT1. A significant difference in NS expression between poor-risk and better-risk and between poor-risk and intermediate-risk AML patients was found. Our initial data indicated that NS can be used for tracking minimal residual disease (MRD) and is a helpful guide for treatment.
    Leukemia research 09/2013; 37(12). DOI:10.1016/j.leukres.2013.09.023 · 2.35 Impact Factor
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    ABSTRACT: The potential of using the waste from simultaneous NOx and SO2 purification bio-trickling process to leach copper ore and to make fertilizer was investigated. It was found there were two main exhausted by-products in the purification process: the acidic liquid in desulfurization tower and the alkalescent liquid in denitrogenation tower. Through operation of leaching the oxide ore sample containing 2.58% copper by the acidic liquid, the effective grade of the metal to be extracted reached 28.37% by weight. With adding proportional dose of ammonia into the alkalescent liquid or the acidic liquid and going through evaporation and concentration, crystal products of ammonium sulfate and ammonium nitrate were generated, which can be used in fertilizer manufacturing.
    09/2013; 779-780:1220-1223. DOI:10.4028/
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    ABSTRACT: Objectives: This study aimed to explore why one acute promyelocytic leukemia (APL) patient underwent complete molecular remission in the persistent presence of the t (2; 3) (p25; q21) karotype. Methods: One APL patient overexpressed PML/RARα (bcr1) and WT1 genes in the presence of the Fms-like tyrosine kinase-internal tandem duplication mutation, while cytogenetics showed t (2; 3) (p25; q21) and t (15; 17) (q22; q21). Cytogenetics and molecular biology were monitored throughout the treatment. Results: After 5 weeks of induction chemotherapy, this case gained complete molecular biology remission with the presence of t (2; 3) (p25; q21). This status was still present during the follow-up consolidate and maintenance therapy. Conclusion: For this patient, t (2; 3) (p25; q21) may be one kind of balanced translocation that leads to miscarriages or causes abnormalities in children, unrelated to leukemia or other malignancies.
    Expert Opinion on Therapeutic Targets 08/2013; 17(10). DOI:10.1517/14728222.2013.832205 · 5.14 Impact Factor
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    ABSTRACT: Increased bone marrow (BM) hematogones (HGs) are often observed in patients with marrow regenerating status. Many studies have focused on the role of HGs in acute lymphoblastic leukaemia (ALL), but very little has been done to understand their effects on acute myeloid leukaemia (AML). Through immunophenotyping, HGs were quantified in 471 BM samples from 292 postchemotherapy AML cases. These samples were analysed to determine whether there is any relationship between HGs percentages and French-American-British (FAB) subtypes or risk stratification of AML. HGs were identified in 57.75% of 471 patient samples (271) with a mean percentage of 3.87 ± 0.25%. No significant differences were found amongst different FAB subtypes of AML (P > 0.05). However, significant differences (P < 0.05) in HG numbers were noted between AML patients experiencing haematological complete remission (HCR) and those who have relapsed. HGs were identified in 59.9% of samples under HCR with a mean per cent of 3.98 ± 0.31%, and 36.7% of individuals who have relapsed have detectable HGs with a mean per cent of 1.75 ± 0.47. In addition, HGs in patients groups with low risk or intermediate risk were elevated when compared with high-risk groups (P < 0.05), whilst no significant difference was found between low-risk patients and intermediate-risk patients (P > 0.05). Patients with >0.1% of HGs had a significantly better median leukaemia-free survival (LFS) and overall survival (OS) than those with <0.1% of HGs (P < 0.01). Therefore, our data indicate that HGs in bone marrow may be used as a favourable prognostic factor that predict for a better outcome of AML patients.
    European Journal of Clinical Investigation 08/2013; 43(11). DOI:10.1111/eci.12151 · 2.73 Impact Factor
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    ABSTRACT: A cyanoethylated wastepaper-degrading bacterial strain S11 was screened from the surface humus soil in the forest of Yunnan University. The bacterium was identified through analysis of morphological, biological and biochemical features and 16S rRNA sequence. Si 1 enjoyed above 99% homology with Ochrobactrum sp.CRRI29's 16S rDNA sequence, therefore the baterium belonged to Ochrobactrum sp. The research on degradation features of S11 revealed that S11 bacterium could effectively degrade cyanoethylated wastepaper under 35 degrees C, pH 11.0, 25% inoculum size and 50g/L substrate concentration; under the optimum conditions, the degradation rate of cyanoethylated wastepaper was 46.83% after degradation by S11 bacterium for 192 hours.
    Applied Mechanics and Materials 04/2013; 316-317:349-353. DOI:10.4028/ · 0.15 Impact Factor

Publication Stats

548 Citations
195.14 Total Impact Points


  • 2010–2015
    • Wuhan Union Hospital
      Wu-han-shih, Hubei, China
  • 2014
    • Harbin Institute of Technology
      Charbin, Heilongjiang Sheng, China
  • 2001–2014
    • Huazhong University of Science and Technology
      • Department of Hematology
      Wu-han-shih, Hubei, China
    • Tongji Hospital
      Wu-han-shih, Hubei, China
  • 2013
    • Yunnan University
      Yün-nan, Yunnan, China
  • 2011
    • Tianjin Medical University Cancer Institute and Hospital
      T’ien-ching-shih, Tianjin Shi, China
  • 2000–2009
    • Tongji Medical University
      • • Institute of Hematology
      • • Department of Hematology
      Wu-han-shih, Hubei, China
  • 2008
    • Wuhan University of Science and Technology
      Wu-han-shih, Hubei, China
  • 2005
    • Wuhan Children's Hospital
      Wu-han-shih, Hubei, China
  • 2004
    • Zhejiang University
      • School of Medicine
      Hangzhou, Zhejiang Sheng, China
  • 2003
    • Wuhan University
      • Department of Pediatrics
      Wu-han-shih, Hubei, China
    • Soochow University (PRC)
      Wu-hsien, Jiangsu Sheng, China