[show abstract][hide abstract] ABSTRACT: We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the library used in this study, candidates of specific binders were identified. In addition, a selected peptide stabilized the G-quadruplex structure of a DNA oligonucleotide derived from the promoter region of the protooncogene c-MYC. This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. Such G-quadruplex peptide binders could be functionally modified to enable switching, cellular penetration, and organelle-targeting for cell and tissue engineering.
[show abstract][hide abstract] ABSTRACT: This study identified risk factors for pressure ulcer development early in the admission stage to determine what appropriate interventions might be conducted early in the admission stage to decrease the risk of pressure ulcer development.
Among patients who develop pressure ulcers, 8-40% of them occur in a critical care setting. Therefore, the development of pressure ulcers is a common problem.
Prospective cohort study.
The study facility was a 606-bed tertiary care hospital. Ninety eight patients admitted to the intensive care unit (ICU) or the high care unit (HCU) from 16 April 2003-15 July 2003 were evaluated in the final analysis.
The mean patient age was 62.3 (SD 16.1) years, and the incidence of pressure ulcers in this study was 11.2% during the observation period. Multivariate analysis showed that 'emergency ICU/HCU patients' and 'infrequent turning' were related to pressure ulcer development. Patients with pressure ulcers experienced significantly fewer turns and repositionings (OR = 0.452, 95% CI: 0.212-0.966], p < 0.05. Fewer pressure ulcers developed in scheduled ICU/HCU patients than in emergency ICU/HCU patients (OR = 0.041 [95% CI: 0.004-0.470], p < 0.01).
There was no relationship between pressure ulcer development and APACHE II score, or any medication that affected skin integrity. The frequency of turning and repositioning and patients with an emergency admission to the ICU/HCU can be the prognostic indicators for developing scoring system in critical care settings.
These patients admitted directly to ICU or HCU were in a high risk group, further preventive strategies will be required.
Journal of Clinical Nursing 02/2010; 19(3-4):414-21. · 1.32 Impact Factor
[show abstract][hide abstract] ABSTRACT: This study aims to identify the factors associated with community health service utilization for menopausal symptoms among Japanese women in urban areas by using Andersen's behavioral model of health service use.
A cross-sectional study was conducted.
The sample comprised 350 women aged 40-60 years living in a community in Japan.
The independent variables were predisposing (demographics, social status, and health beliefs), enabling (family and community resources), and need factors (perceived health status and the need for community health services). The dependent variable was community health service utilization. A logistic regression model was used.
8 of the women were categorized in "treatment required" by simplified menopausal index score, and 103 women said that they would use the services. Knowledge regarding the services, OR: 1.814 (1.084-3.036); the absence of a negative attitude toward menopausal syndromes, OR: 0.605 (0.367-0.998); and the presence of a positive attitude, OR: 2.203 (1.070-4.536); influenced community health service utilization.
An appropriate attitude toward menopausal symptoms was observed to be more relevant to community health service utilization than the experience or acuity of the menopausal symptoms themselves. Therefore, community-dwelling, middle-aged women should be provided with sufficient education regarding menopausal symptoms to encourage their utilization of community health services.
Public Health Nursing 06/2009; 26(3):229-39. · 0.78 Impact Factor
[show abstract][hide abstract] ABSTRACT: This paper is a report of a study conducted to examine whether continuous interface pressure monitoring of postoperative patients in an intensive care unit is feasible in clinical practice.
The interface pressure between skin and surfaces is generally evaluated for pressure ulcer prevention. However, the intensity and duration of interface pressure necessary for pressure ulcer development remains unclear because the conventional interface pressure sensors are unsuitable for continuous monitoring in clinical settings.
A total of 30 postoperative patients in an intensive care unit participated in this study in 2006-2007. A sensor was built into a thermoelastic polymer mattress. The whole-body interface pressure was recorded for up to 48 hours. Pressure ulcer development was observed during the morning bed-bath. For analysis, the intensity and duration of the maximal interface pressure was evaluated.
The mean age of the study group was 62.0 +/- 15.4 years. Two participants developed stage I pressure ulcer and blanchable redness at the sacrum. The longest duration of pressures greater than 100 mmHg were 487.0, 273.5 and 275.7 minutes in the pressure ulcer, blanchable redness and no redness groups respectively.
Continuous monitoring of the intensity and duration of whole-body interface pressure using the KINOTEX sensor is feasible in intensive care patients.
Journal of Advanced Nursing 03/2009; 65(4):809-17. · 1.53 Impact Factor
[show abstract][hide abstract] ABSTRACT: The purpose of the present study was to examine the validity of the KINOTEX sensor via comparison with an existing sensor and to determine the sensing areas for a new alternating-air mattress that incorporates an interface pressure-sensing system. The study design was an evaluation study to validate and determine the sensing area of the KINOTEX sensor in comparison with another sensor. Study participants were fifty-one healthy volunteers over eighteen years of age, and the two sensors were placed between participants and an alternating-air mattress. We measured the contact area, full weight load, and maximum pressure in the calcaneal region using two sensors and obtained a graphic pressure distribution of >40 mmHg in the lateral and supine positions. Correlation coefficients between sensors were r = 0.88 (p < 0.001) for the contact area, r = 0.89 (p < 0.001) for full weight load, and r = 0.72 (p < 0.001) at maximum pressure in the calcaneal region. Ninety-one percent of the pressure distribution was recorded in the central 50 cm of the bed, and 94.6% was recorded within an area 160 cm in length need the foot of the bed. We investigated the correlation between the KINOTEX sensor and an existing sensor and determined the necessary sensing area. Results suggested the feasibility of developing a new alternating-air mattress incorporating an interface pressure-sensing system to help prevent pressure ulcers.
[show abstract][hide abstract] ABSTRACT: The effect of vibration on skin microcirculation was studied to investigate the possibility of clinical use of vibration to prevent and treat pressure ulcers. Vibrations at a vibrational intensity of 600, 800, or 1,000 mVpp with a fixed frequency of 47 Hz were applied horizontally to the ear of male hairless mice (n = 6 for each group) under inhalation anesthesia. The control group (n = 6) received no vibrations. Venular blood flow was measured by an intravital videomicroscope at the baseline and at 0, 5, and 15 min after the application of vibrations. A significant increase was observed in the 600 mVpp group 5 and 15 min after vibration in comparison to the control group (P = 0.002 and P = 0.046, respectively). We also detected increased blood flow in the 800 mVpp group (P = 0.028) and the 1,000 mVpp group (P = 0.012) 5 min after vibration; however, these increases attenuated after 15 min. These results indicate that direct skin vibration at a frequency of 47 Hz improves skin blood flow. The present study gives further support to the role of vibration on a short-term increase in skin blood flow.
[show abstract][hide abstract] ABSTRACT: This study examined factors related to the frequency and duration of family visits to elderly residents of nursing homes in Japan. Data were collected using a self-administered questionnaire from 299 family members of residents of three nursing homes in Tokyo. Almost all family members visited residents more than once a month, and more than three quarters stayed for more than 1h during each visit. These findings confirm that family involvement with the elderly is maintained even after admission to a nursing home. Multiple regression analysis revealed the variables related to a higher frequency and duration of visits. Some factors related to frequent or long visits such as the requirement of higher care or the ability to remember family visits were similar to the findings of other studies conducted in western countries, yet the results need to be understood in a Japanese cultural context. Other factors such as the gender of the visitors, distance to the nursing home, and sentiment about placement were thought to be related to the unique cultural norms of family caregiving in Japan. These findings will be useful for developing effective methods to assist nursing home residents and their families in Japan.
Archives of Gerontology and Geriatrics 01/2007; 45(1):73-86. · 1.70 Impact Factor
[show abstract][hide abstract] ABSTRACT: It is relatively rare to encounter a case of neovascular glaucoma induced by malignant lymphoma metastasized into the eyeball.
A 79-year-old woman initially visited our ophthalmology clinic with the chief complaint of blurring of vision in the left eye which was affected by neovascular glaucoma. She had a history of systemic malignant lymphoma, first diagnosed from skin biopsy and treated to complete remission 3 years previously. A metastatic brain lesion, detected 2 months before her initial visit to our clinic, was cured by radiotherapy.
By ultrasound biomicroscopy (UBM) we detected abnormal thickening of the temporal half of the ciliary body of her left eye. Cytological examination of the aqueous humor revealed invasion by malignant cells of presumed lymphocyte origin. Radiation therapy to the left eye normalized the intraocular pressure in a week, followed by a reduction in the neovascularization of the iris and the thickening of the ciliary body.
This case showed that metastatic malignant lymphoma in the eye could result in neovascular glaucoma and that UBM is useful to detect and to observe lesions in the iris and ciliary body.
[show abstract][hide abstract] ABSTRACT: The purpose of this study was to examine the mental health and related factors with family caregivers for the elderly in special-care nursing homes for the aged.
We conducted a questionnaire survey among family caregivers for elderly living in three special-care nursing homes in Tokyo. The questionnaire included factors from the General Health Questionnaire-28 (GHQ-28) pertaining to family caregivers, the elderly, and the relationships between them. The GHQ-28 was used to measure the mental health of the caregivers and a logistic regression model was applied for the analysis.
Of the 145 family caregivers surveyed, 59 (40.7%) exhibited low mental health (GHQ-28> or = 7). The logistic regression analysis revealed that family caregivers with low mental health had lower social support (OR: 0.10 (0.03-0.29)) and had longer visiting times (OR: 5.80 (1.79-18.82)).
The results suggested that many family caregivers for elderly persons in institutions may have poor mental health and that it is necessary to provide them with support. It is concluded that self-help groups for families and the promotion of communication between the elderly and their family caregivers might be effective for this purpose.
[Nippon kōshū eisei zasshi] Japanese journal of public health 05/2005; 52(5):399-410.
[show abstract][hide abstract] ABSTRACT: To analyze retinal nerve fiber layer (RNFL) thickness in eyes with band atrophy by use of optical coherence tomography (OCT) and to evaluate the ability of OCT to detect this characteristic pattern of RNFL loss.
Cross-sectional, retrospective study.
Thirty-four eyes of 18 patients with bitemporal hemianopia caused by optic chiasm compression by chiasmal tumors were studied. All eyes were divided into 3 groups according to visual field loss grading after Goldmann perimetry.
Retinal nerve fiber layer thickness measurements with OCT.
Retinal nerve fiber layer thickness around the optic disc was measured by OCT (3.4-mm diameter circle). Calculation of the changes in OCT parameters, including the horizontal (nasal + temporal quadrant RNFL thickness) and vertical values (superior + inferior quadrant RNFL thickness) was based on data from 160 normal eyes. Comparison between the 3 visual field grading groups was done with the analysis of variance test. The receiver operating characteristic (ROC) curve for the horizontal and vertical value were calculated, and the areas under the curve (AUC) were compared.
Retinal nerve fiber layer thickness in eyes with band atrophy decreased in all OCT parameters. The reduction rate in average and temporal RNFL thickness and horizontal value was correlated with visual field grading. The AUC of horizontal value was 0.970+/-0.011, which was significantly different from AUC of vertical value (0.903+/-0.022).
The degree of RNFL thickness reduction correlated with that of visual field defects. Optical coherence tomography was able to identify the characteristic pattern of RNFL loss in these eyes.
[show abstract][hide abstract] ABSTRACT: A 17-year-old girl presented with bilateral angle-closure glaucoma associated with spherophakia. A previous bilateral laser iridotomy failed to control intraocular pressure (IOP). Goniosynechialysis with lens aspiration and posterior chamber intraocular lens implantation were performed in both eyes. Peripheral iridoplasty was performed 3 days later. The postoperative IOP was controlled without medication for 12 months in the right eye and 24 months in the left eye. By restructuring the physiologic aqueous outflow route, goniosynechialysis safely and effectively treated secondary glaucoma from spherophakia.
Journal of Cataract [?] Refractive Surgery 03/2004; 30(2):513-6. · 2.53 Impact Factor
[show abstract][hide abstract] ABSTRACT: We investigated whether passive leg cycle exercise (PLE) for the elderly and patients with spinal cord injuries leads to the modulation of autonomic nervous activity in healthy female subjects. To evaluate changes in autonomic nervous activity during PLE, we used autoregressive (AR) power spectra of pulse interval variability (PIV) corresponded to R-R interval variability and systolic blood pressure variability (SPV). Our results showed that LF and HF powers and the ratio power (LF/HF) of PIV reflecting cardiac sympathetic, parasympathetic tone and sympathovagal balance were not changed during PLE, but during recovery period immediately after PLE, LF and HF powers and total power of PIV was significantly (p