[Show abstract][Hide abstract] ABSTRACT: Liver fluke infections in herbivores are common in many countries, including Iran. Meat-inspection records in an abattoir located in Ahwaz (capital of Khuzestan Province, in southwestern Iran), fromÂ March, 20, 1999 toÂ March, 19, 2008 were used to determine the prevalence and long term trend of liver fluke disease in sheep, goats and cattle in the region. A total of 3186755 livestock including 2490742 sheep, 400695 goats and 295318 cattle were slaughtered in the 9-year period and overall 144495 (4.53%) livers were condemned. Fascioliasis and dicrocoeliosis were responsible for 35.01% and 2.28% of total liver condemnations in this period, respectively. Most and least rates of liver condemnations due to fasciolosis in slaughtered animals were seen in cattle and sheep, respectively. The corresponding figures from dicrocoeliosis were goats and sheep, respectively. The overall trend for all livestock in liver fluke was a significant downward during the 9- year period. The prevalence of liver condemnations due to fasciolosis decreased from 7.37%, 1.80%, and 4.41% in 1999â€“2000 to 4.64%, 1.12%, and 2.80% in 2007â€“2008 for cattle, sheep and goats, respectively. Dicrocoeliosis was less prevalent than fasciolosis, but similarly declined from 0.35 % and 0.15% in 1999â€“2000 to 0.00% and 0.08 % in 2007â€“2008 in cattle and sheep, respectively. Data showed significant seasonal pattern for Dicrocoelium dendriticum in sheep and goats, but for Fasciola spp. in different animals there were no statistically significant differences with respect to season. Liver condemnations due to fasciolosis and dicrocoeliosis were more prevalent in cattle slaughtered during summer, whereas they were higher in winter for both sheep and goats. The odds ratio showed a slightly different pattern in some years; however, the overall declining trend was still observed. This survey provides baseline data for the future monitoring of these potentially important parasitic infections in the region, and demonstrating possible long term trends.
[Show abstract][Hide abstract] ABSTRACT: A field evaluation of the formalin-gasoline procedure to detect parasite ova, cysts, or larvae from 470 fecal specimens (comprising both fresh and formalin-preserved stool samples) was compared with that of the formalin-ether sedimentation technique. Parallel concentrates with diethyl ether and gasoline were prepared for each specimen, and the species and appearance of recovered parasite species were determined. Of 470 total specimens, 206 (43.83%) were found to be positive for parasites in one or both concentration techniques. Gasoline was comparable to diethyl ether in the recovery of parasite eggs, cysts, and larvae, so that the formalin-gasoline and the formalin-diethyl ether sedimentation techniques detected 165 and 156 positive of total specimens, respectively. In this study, gasoline proved to be as good as diethyl ether in concentrating parasite eggs and cysts, as well as in maintaining characteristic morphology. However, gasoline was considerably superior to diethyl ether in detecting larvae of Strongyloides stercoralis. Parallel examination of total stool samples by the routine formalin-ether (original) and by the formalin-gasoline techniques resulted in identical distribution of positive slides and morphology of recovered parasite species. However, the easy availability of gasoline (wherever gas stations are present) and its low cost in comparison to ether makes gasoline superior to ether for use in concentration of stools by the sedimentation method in laboratories, including laboratories with limited material resources and also laboratories present in small cities and rural health centers.
Parasitology Research 11/2008; 104(3):553-7. · 2.85 Impact Factor