Nam Jin Lee

Chungbuk National University, Chinsen, North Chungcheong, South Korea

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Publications (13)80.77 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The Maillard reaction products are known to be effective in chemoprevention. Here, we focused on the anti-cancer effects of (E)-2,4-bis(p-hydroxyphenyl)-2-butenal on in vitro and in vivo colon cancer. We analysed the anti-cancer activity of (E)-2,4-bis(p-hydroxyphenyl)-2-butenal on colon cancer cells by using cell cycle and apoptosis analysis. To elucidate it's mechanism, NF-κB DNA binding activity, docking model as well as pull-down assay. Further, a xenograft model of colon cancer was studied to test the in vivo effects of (E)-2,4-bis(p-hydroxyphenyl)-2-butenal. (E)-2,4-Bis(p-hydroxyphenyl)-2-butenal inhibited colon cancer cells (SW620 and HCT116) growth followed by induction of apoptosis in a concentration-dependent manner via down-regulation of NF-κB activity. In docking model as well as pull-down assay, (E)-2,4-bis(p-hydroxyphenyl)-2-butenal directly binds to three amino acid residues of IKKβ, thereby inhibited IKKβ activity in addition to induction of death receptor 6 (DR6) as well as their target apoptotic genes. Finally, (E)-2,4-bis(p-hydroxyphenyl)-2-butenal suppressed anchorage-independent cancer cell growth, and tumor growth in xenograft model accompanied with apoptosis through inhibition of IKKβ/NF-κB activity, and overexpression of DR6. These results suggest that (E)-2,4-bis(p-hydroxyphenyl)-2-butenal inhibits colon cancer cell growth through inhibition of IKKβ/NF-κB activity and induction of DR6 expression.
    Apoptosis 09/2013; 19(1). DOI:10.1007/s10495-013-0903-x
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    ABSTRACT: High doses of acetaminophen (APAP; N-acetyl-p-aminophenol) cause severe hepatotoxicity after metabolic activation by cytochrome P450 2E1. This study was undertaken to examine the preventive effects of thiacremonone, a compound extracted from garlic, on APAP-induced acute hepatic failure in male C57BL/6J. Mice received with 500 mg/kg APAP after a 7-day pretreatment with thiacremonone (10-50 mg/kg). Thiacremonone inhibited the APAP-induced serum ALT and AST levels in a dose-dependent manner, and markedly reduced the restricted area of necrosis and inflammation by administration of APAP. Thiacremonone also inhibited the APAP-induced depletion of intracellular GSH, induction of nitric oxide, and lipid peroxidation as well as expression of P450 2E1. After APAP injection, the numbers of Kupffer cells, natural killer cells, and cytotoxic T cells were elevated, but the elevated cell numbers in the liver were reduced in thiacremonone pretreated mice. The expression levels of I-309, M-CSF, MIG, MIP-1 α , MIP-1 β , IL-7, and IL-17 were increased by APAP treatment, which were inhibited in thiacremonone pretreated mice. These data indicate that thiacremonone could be a useful agent for the treatment of drug-induced hepatic failure and that the reduction of cytotoxic immune cells as well as proinflammatory cytokine production may be critical for the prevention of APAP-induced acute liver toxicity.
    Evidence-based Complementary and Alternative Medicine 07/2013; 2013:974794. DOI:10.1155/2013/974794
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    ABSTRACT: This study was performed to investigate whether IH901, a ginseng intestinal metabolite, administration has the ability to ameliorate exercise-induced oxidative stress and reserving antioxidant defense capability in rat skeletal muscles and lung. Eight adult male SD rats per group were randomly assigned to the resting control (RC), exercise control (EC), resting with IH901 (25, 50, and 100 mg/kg) consumption (R/IH901), and exercise with IH901 (25, 50, and 100 mg/kg) consumption (E/IH901). The trained groups ran 35 min 2 days/week for 8 weeks. To analyze the IH901-training interaction, serum biochemical analysis, lipid peroxidation (thiobarbituric acid reactive substance; protein carbonyl), citrate synthase, protein oxidation (xanthine oxidase; myeloperoxidase; nitric oxide), antioxidant (glutathione peroxidase; glutathione S-transferase) and superoxide dismutase in skeletal muscles and lung tissue were performed. Compared to the EC, the animals consumed IH901 had significantly increased exercise endurance times (p<0.05) and decreased plasma creatine kinase and lactate dehydrogenase levels (p<0.05). E/IH901 groups increased citrate synthase and anti-oxidant enzymes and decreased lipid peroxidation and protein oxidation (p<0.05). In conclusion, IH901 consumption in aging rats after eccentric exercise has beneficial effects on potent anti-inflammatory and anti-oxidant activities through down-regulation of pro-inflammatory mediators, lipid peroxidation, and protein oxidation and up-regulation of anti-oxidant enzymes.
    Journal of veterinary science (Suwŏn-si, Korea) 06/2013; 14(3). DOI:10.4142/jvs.2013.14.3.249
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    ABSTRACT: Carbon composite bipolar plates with various carbon black contents were prepared by a compression molding method. The electrical conductivity and electrochemical stability of the bipolar plates have been evaluated. It is found that the electrical conductivity increases with increasing carbon black contents up to 15 wt %. When the carbon black contents are greater than 15 wt %, the electrical conductivity decreases because of a poor compatibility between epoxy resin and carbon black, and a weakening of compaction in the carbon composite bipolar plate. Based on the results, it could be concluded that there are optimum carbon black contents when preparing the carbon composite bipolar plate. Corrosion tests show that the carbon composite bipolar plate with 15 wt % carbon black exhibits better electrochemical stability than a graphite bipolar plate under a highly acidic condition. When the optimized carbon composite bipolar plate is applied to vanadium redox flow cells, the performance of flow cells with the carbon composite bipolar plate is comparable to that of flow cells with the graphite bipolar plate.
    Bulletin- Korean Chemical Society 11/2012; 33(11):3589-3592. DOI:10.5012/bkcs.2012.33.11.3589
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    ABSTRACT: To evaluate the significance of C-C chemokine receptor type 5 (CCR5) in lung tumor development, we compared carcinogen-induced tumor growth in CCR5 knockout (CCR5(-/-)) mice and wild-type (CCR5(+/+)) mice. CCR5(-/-) mice showed reduced urethane (1g/kg)-induced tumor incidence when compared with those of CCR5(+/+) mice. We investigated the activation of nuclear factor-kappaB/STAT3 since these are implicated transcription factors in the regulation of genes involving tumor growth. Significant inhibition of DNA-binding activity of nuclear factor-kappaB and STAT3, and the translocation of p50 and p65 into the nucleus and the phosphorylation of IĸB were found in the lungs of CCR5(-/-) mice compared with the lungs of CCR5(+/+) mice. Expression of apoptotic protein such as cleaved caspase-3, cleaved PARP and Bax was elevated, whereas the expression levels of survival protein such as Bcl-2 and cIAP1 was decreased in the lungs of CCR5(-/-) mice. Interestingly, we found that the level of monocyte chemoattractant protein-1 (MCP-1), a tumor growth-promoting cytokine, was significantly reduced in the lung tumor tissue and blood of CCR5(-/-) mice compared with the level in CCR5(+/+) mice. In addition, CCR5 small interfering RNA (siRNA) and inhibitor of MCP-1 blocked lung cancer cell growth, which was abolished by the addition of MCP-1 protein in cultured lung cancer cells. Moreover, inactivation of CD8(+) cytotoxic T cell and dendritic cells was significantly increased in the blood, lung tumors and spleens of CCR5(-/-) mice compared with that of CCR5(+/+) mice. Therefore, these results showed that CCR5 deficiency suppressed lung tumor development through the inhibition of nuclear factor-kappaB/STAT3 pathways and the downregulation of MCP-1 in the carcinogen-induced lung tumor model.
    Carcinogenesis 08/2012; DOI:10.1093/carcin/bgs265
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    ABSTRACT: A new approach for enhancing the electrochemical performance of carbon felt electrodes by employing non-precious metal oxides is designed. The outstanding electro-catalytic activity and mechanical stability of Mn(3)O(4) are advantageous in facilitating the redox reaction of vanadium ions, leading to efficient operation of a vanadium redox flow battery.
    Chemical Communications 04/2012; 48(44):5455-7. DOI:10.1039/c2cc31433a
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    ABSTRACT: The electrochemistry of a non-aqueous redox flow battery employing nickel and iron bipyridine in tetraethylammonium tetrafluoroborate and propylene carbonate has been investigated. Ni(0)/Ni(II) and Fe(II)/Fe(III) redox couples showed reversible reactions for negative and positive electrodes, respectively. Various metal electrodes (Pt, Ir, Ni, Cu, Mo, W, Nb, Co, and Ti) were evaluated for those couples using cyclic voltammetry. Based on the analysis of the cyclic voltammograms, the reactivity and kinetics for the electrochemical reaction of metal complexes were found to strongly depend on the type of metal electrodes. Among non-precious metals, Cu and Mo showed relatively better electrocatalytic activities as negative electrodes for the Ni(0)/Ni(II) redox couple and Ni was the best material as a positive electrode for the Fe(II)/Fe(III) redox couple in terms of electrochemical reactivity and reaction kinetics.
    Electrochemistry Communications 09/2011; 13(9):997–1000. DOI:10.1016/j.elecom.2011.06.022
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    ABSTRACT: This study aimed to validate the TICS and modified TICS (TICSm) in Korean elderly population and to compare MCI and dementia screening ability between TICS and TICSm. TICS and TICSm were administered to 70 cognitively normal (CN), 75 MCI, and 85 dementia subjects, with mini-mental state examination (MMSE) and other cognitive and functional measures. TICS and TICSm scores were highly correlated with other global cognitive and functional scores. The CN vs. dementia discrimination ability of both instruments was as excellent as that of MMSE (sensitivity/specificity at optimal cutoff: 87.1/90.1 for TICS; 88.2/90.0 for TICSm). Although their CN vs. MCI discrimination performances were comparable to that of MMSE, they were far from perfect (sensitivity/specificity: 69.3/68.6 for TICS; 73.3/67.1 for TICSm). There was no significant difference in dementia or MCI screening accuracy between TICS and TICSm. Both of them also showed high test-retest reliability. Our findings indicate that TICS and TICSm are reliable and as valid as MMSE in regard of screening cognitively impaired elderly. In terms of the comparison between TICSm and TICS, however, TICSm has little advantage over TICS for screening dementia and even MCI, in spite of longer administration time and more efforts required.
    Archives of gerontology and geriatrics 01/2011; 52(1):e26-30. DOI:10.1016/j.archger.2010.04.008
  • Alzheimer's and Dementia 07/2009; 5(4). DOI:10.1016/j.jalz.2009.04.228
  • Alzheimer's and Dementia 07/2009; 5(4). DOI:10.1016/j.jalz.2009.04.852
  • Alzheimer's and Dementia 07/2008; 4(4). DOI:10.1016/j.jalz.2008.05.1584
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    ABSTRACT: It has been suggested that oxidative injuries have an important role in the pathogenesis of Alzheimer's disease (AD). In this study, we assess whether the plasma levels of albumin, bilirubin, and uric acid would be altered in the AD patients compared to those of the healthy controls. Furthermore, we tried to find the correlations between plasma antioxidant levels and the cognitive function in AD patients. The plasma albumin, bilirubin, and uric acid levels were measured by standard methods in 101 AD patients and 101 healthy controls. The Korean version of the Mini Mental Status Examination (MMSE-K) was used to evaluate the cognitive functions of AD patients. A significant reduction in the albumin, bilirubin, and uric acid levels in the AD group was found compared to those of the control group. The scores of MMSE-K showed the positive correlation with albumin level in the AD group after adjusting confounding factors such as body mass index, gender and age. This study showed that oxidative injuries could be involved in the pathogenesis of AD, as well as indicating that some antioxidant might be associated with the cognitive functions in AD.
    International Journal of Geriatric Psychiatry 04/2006; 21(4):344-8. DOI:10.1002/gps.1469
  • Article: P2-249
    Chang Uk Lee, Nam Jin Lee

Publication Stats

126 Citations
80.77 Total Impact Points


  • 2013
    • Chungbuk National University
      • College of Pharmacy
      Chinsen, North Chungcheong, South Korea
  • 2012
    • Korea University
      • Department of Materials Science and Engineering
      Sŏul, Seoul, South Korea
  • 2011–2012
    • Korea Electronics Technology Institute
      • Advanced Batteries Research Center
      Sŏngnam, Gyeonggi Province, South Korea
  • 2006
    • University of Surrey
      • Centre for Vision, Speech and Signal Processing (CVSSP)
      Guilford, England, United Kingdom