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Maria A Rueger, Mitra Ameli,
Hongfeng Li,
Alexandra Winkeler,
Benedikt Rueckriem,
Stefan Vollmar,
Norbert Galldiks,
Volker Hesselmann,
Cornel Fraefel,
Klaus Wienhard,
Wolf-Dieter Heiss,
Andreas H Jacobs
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ABSTRACT: The purpose of this study was to investigate the potential of 3'-deoxy-3'-[¹⁸F]fluorothymidine ([¹⁸F]FLT) positron emission tomography (PET) to detect early treatment responses in gliomas. Human glioma cells were stably transduced with genes yielding therapeutic activity, sorted for different levels of exogenous gene expression, and implanted subcutaneously into nude mice. Multimodality imaging during prodrug therapy included (a) magnetic resonance imaging, (b) PET with 9-(4-[¹⁸F]fluoro-3-hydroxymethylbutyl)guanine assessing exogenous gene expression, and (c) repeat [¹⁸F]FLT PET assessing antiproliferative therapeutic response. All stably transduced gliomas responded to therapy with significant reduction in tumor volume and [¹⁸F]FLT accumulation within 3 days after initiation of therapy. The change in [¹⁸F]FLT uptake before and after treatment correlated to volumetrically calculated growth rates. Therapeutic efficacy as monitored by [¹⁸F]FLT PET correlated to levels of therapeutic gene expression measured in vivo. Thus, [¹⁸F]FLT PET assesses early antiproliferative effects, making it a promising radiotracer for the development of novel treatments for glioma.
Molecular imaging and biology: MIB: the official publication of the Academy of Molecular Imaging 06/2011; 13(3):547-57. · 2.47 Impact Factor
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Maria A. Rueger, Mitra Ameli,
Hongfeng Li,
Alexandra Winkeler,
Benedikt Rueckriem,
Stefan Vollmar,
Norbert Galldiks,
V Hesselmann,
Cornel Fraefel,
Klaus Wienhard,
Wolf-Dieter Heiss,
Andreas Jacobs
Molecular Imaging and Biology.
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Maria A. Rueger, Mitra Ameli,
Hongfeng Li,
Alexandra Winkeler,
Benedikt Rueckriem,
Stefan Vollmar,
Norbert Galldiks,
V Hesselmann,
Cornel Fraefel,
Klaus Wienhard,
Wolf-Dieter Heiss,
Andreas H. Jacobs
Molecular Imaging and Biology.
