Mischo Kursar

Publications (3)8.03 Total impact

• Source

  Available from: ncbi.nlm.nih.gov

  Article: Tumour class prediction and discovery by microarray-based DNA methylation analysis.

  Péter Adorján, Jürgen Distler, Evelyne Lipscher, Fabian Model, Jürgen Müller, Cécile Pelet, Aron Braun, Andrea R Florl, David Gütig, Gabi Grabs, [......], Volker Müller, Thomas Otto, Christian Scholz, Wolfgang A Schulz, Hans-Helge Seifert, Ina Schwope, Heike Ziebarth, Kurt Berlin, Christian Piepenbrock, Alexander Olek
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  ABSTRACT: Aberrant DNA methylation of CpG sites is among the earliest and most frequent alterations in cancer. Several studies suggest that aberrant methylation occurs in a tumour type-specific manner. However, large-scale analysis of candidate genes has so far been hampered by the lack of high throughput assays for methylation detection. We have developed the first microarray-based technique which allows genome-wide assessment of selected CpG dinucleotides as well as quantification of methylation at each site. Several hundred CpG sites were screened in 76 samples from four different human tumour types and corresponding healthy controls. Discriminative CpG dinucleotides were identified for different tissue type distinctions and used to predict the tumour class of as yet unknown samples with high accuracy using machine learning techniques. Some CpG dinucleotides correlate with progression to malignancy, whereas others are methylated in a tissue-specific manner independent of malignancy. Our results demonstrate that genome-wide analysis of methylation patterns combined with supervised and unsupervised machine learning techniques constitute a powerful novel tool to classify human cancers.
  Nucleic Acids Research 04/2002; 30(5):e21. · 8.03 Impact Factor
• Article: Ranked matrix analysis of multiple gene methylation patterns in normal human tissue.

  B Genc, J. Lewin, Mischo Kursar, C. Ivascu, Ralf Lesche, Sabine Maier, Jurgen Mueller, Kurt Berlin, Alexander Olek
  American Journal of Human Genetics, v.71, 4 Suppl. S, 56-56 (2002).
• Article: Tumour class prediction and discovery by microarray-based DNA methylation analysis

  Peter Adorjan, Jurgen Distler, Evelyne Lipscher, Fabian Model, Jurgen Muller, Cecile Pelet, Aron Braun, Andrea R Florl, David Gutig, Gabi Grabs, [......], Volker Muller, Thomas Otto, Christian Scholz, Wolfgang A Schulz, Hans H. Seifert, Ina Schwope, Heike Ziebarth, Kurt Berlin, Christian Piepenbrock, Alexander Olek
  Nucleic Acids Research, v.30, e21-e21 (2002).