M Sobaniec-Lotowska

Medical University of Bialystok, Belostok, Podlasie, Poland

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Publications (52)12.13 Total impact

  • Krzysztof Sendrowski · Wojciech Sobaniec · Piotr Sobaniec · Maria E Sobaniec-Lotowska
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    ABSTRACT: Valproate (VPA) is a widely used antiepileptic drug. A serious neurological-outcome defined as valproate encephalopathy (VE) may rarely occur during VPA therapy. Structural abnormalities within neurons are postulated as one of the reasons for VE. The aim of this study was to assess the ultrastructure of neurons in the hippocampal cortex during the course of chronic application of VPA to rats. VPA was chronically administered to rats, intragastrically, once daily at a dose of 200 mg/kg b.w. for 1, 3, 6, 9 and 12 months. The samples of hippocampal cortex, after routine laboratory preparation, were examined by electron microscopy. The drug induced pronounced ultrastructural changes in the population of pyramidal neurons within the hippocampal cortex after 9 and 12 months of VPA administration. The most expressed abnormalities were observed within the mitochondria and manifested by fragmentation of crests and almost complete disappearance of intramitochondrial granules. Mitochondria of numerous neurons resembled large vacuolar structures. Widening, shortening and irregular distribution of rough endoplasmic reticulum was also found. A characteristic feature of damaged neurocytes in the last two phases of the experiment was the disintegration of nuclear chromatin and the presence of numerous lipofuscin deposits within hyaloplasm. These cells assumed the look of "dark neurons" and presented the ultrastructural features of apoptosis and necrosis. Our results indicate that long-term VPA administration to rats leads to aponecrosis of hippocampal neurons. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 31-37).
    Folia Histochemica et Cytobiologica 04/2013; 51(1):31-37. DOI:10.5603/FHC.2013.005 · 1.36 Impact Factor
  • Pharmacological reports: PR 09/2008; 35(1). DOI:10.1055/s-0028-1086725 · 1.93 Impact Factor
  • Source
    M Barwijuk-Machała · J Reszeć · A Kemona · M Sobaniec-Lotowska
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    ABSTRACT: The aim of the study was to evaluate the correlation of c-erb-b2 and Bcl-xl expression in biopsy specimens of Barrett's oesophagus from 44 patients with morphological features. The examined group was subdivided into: negative for dysplasia, indefinite for dysplasia, positive for dysplasia-low grade, and adenocarcinoma with high grade dysplasia. Positive c-erb-B2 staining was found in 34.1% and Bcl-xl protein expression was observed in 96.9% of BE. The results showed increased c-erb-B2 and Bcl-xl protein expressions with progressive grades of dysplasia to adenocarcinoma. In conclusion, an evaluation of c-erb-B2 and Bcl-xl expression can be useful for the histopatologic diagnosis of BE and correct interpretation of dysplasia.
  • M Sobaniec-Lotowska · W Sobaniec · A Augustynowicz
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    ABSTRACT: Morphometric analysis of the cerebellar cortex capillary cross-section area performed in experimental valproate encephalopathy using transmission electron microscopy showed that prolongation of VPA application resulted in more enhanced lumen narrowing manifested in gradual reduction in the mean value of the coefficient examined. After 6, 9 and 12 months of experiment this value was statistically different from that obtained in control subgroups, being respectively lower by approximately 22%, 48% and 65%. One month after terminating of chronic administration this value was close to the one found after 12 months of the study. Three months after the drug withdrawal the coefficient was higher by approximately 44% compared to the one after 12 months, which seemed to indicate an increase in capillary lumen patency. The morphometric analysis of the cerebellar cortex capillary cross-section area performed in the present study objectifies the results of qualitative ultrastructural investigations concerning the microcirculation of this CNS structure.
    Folia Neuropathologica 02/2001; 39(4):277-80. · 1.57 Impact Factor
  • B Zalewski · W Famulski · M Sulkowska · M Sobaniec-Lotowska · Z Piotrowski · W Kisielewski · S Sulkowski
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    ABSTRACT: CD44 is a cell adhesion molecule involved in tumour growth and progression. This study was undertaken to evaluate the expression of CD44 standard protein in a series of 54 colorectal adenocarcinomas in correlation with cathepsin D immunoreactivity and some other clinicopathological variables. Formalin-fixed and paraffin-embedded tissues were investigated with anti-CD44 standard protein and anti-cathepsin D antibody. Immunolocalisation of CD44 protein and cathepsin D was performed using LSAB method. 13 (41.9%) out of 31 carcinomas without lymph-node metastases had positive CD44 expression, whereas only 6 (26.1%) out of 23 carcinomas with lymph-node metastases were found positive for CD44 expression. CD44 expression in carcinomas was positively correlated with tumour cells cathepsin D (p<0.01) immunostaining. statistically significant correlation was found between the expression of CD44 standard protein and the tumour site, age and sex of the patients. These results suggest that the standard-type CD44 protein lymph-node metastases, probably with cooperation of cathepsin D.
    Folia Histochemica et Cytobiologica 01/2001; 39 Suppl 2:152-3. · 1.36 Impact Factor
  • M Barwijuk-Machała · M Sulik · A Kemona · M Sobaniec-Lotowska
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    ABSTRACT: The review of literature concerning gastritis, especially the chronic form has been carried out. Based on published data and own authors experience an application of the Sydney System in differential diagnosis of gastritis was presented.
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    ABSTRACT: The effect of cyclophosphamide (CP) on the ultrastructure of the lung tissue and the activity of angiotensin converting enzyme (ACE) in serum was evaluated in rats. The animals were given cyclophosphamide (CP) in a single intraperitoneal dose of 150 mg/kg b.w. ACE activity was evaluated in the blood serum collected from the left ventricle of the heart using the spectrophometric method. In all time subgroups, the CP-receiving animals showed a decrease in ACE activity. Ultrastructural examinations of CP-treated animals revealed increased adhesion of neutrophiles and monocytes to the damage endothelium of the alveolar septa vessels and focally accumulation of the platelets.
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    ABSTRACT: Some cyclophosphamide toxic effects on lung tissue are presented. Cyclophosphamide metabolism, pathogenesis of lung damage and morphological lung tissue changes caused by that agent were characterized. Attention was focused on BAL evaluation as a useful method in the monitoring of lung tissue damage degree.
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    ABSTRACT: Septic shock is a catastrophic consequence of invasive infection. Unfortunately, recent advances in surgical and medical sciences have not significantly reduced the overall mortality from septic shock. Bacterial antigens stimulate a cascade of cytokine release; each cytokine helps the host to overcome infection, but their excessive production causes them to trigger events that lead to septic syndrome and shock. Tumour necrosis factor (TNF-alpha) has a pivotal role in orchestrating the events leading to septic shock. Intraperitoneal administration of certain substances can increase the number and phagocytic activity of cells, which reach naturally the site of infection. Activation of the immunity cells in the peritoneal cavity and their immunocompetence are found to be responsible for the organism protection against abdominal cavity infections. Macrophages, lymphocytes and granulocytes of low activity in the non-stimulated peritoneal cavity become significant due to the influence of numerous biologically active substances. This study was designed to determine the peritoneal response to local administration of LPS or TNF-alpha in the course of experimental septic shock.
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    ABSTRACT: Cyclophosphamide (CP) is one of the widely used cytostatic drugs, with a strong toxic influence on pulmonary tissue. Experimental works have shown that a single high dose of CP causes injury to all elements of the interalveolar septum, especially to the alveolar endothelial and epithelial cells. The aim of this work was to evaluate the influence of pentoxifylline (PTXF) (30 mg/kg b.w.) on the ultrastructure of lungs capillaries and blood cell count as well as plasma fibrinogen levels in Wistar rats after intraperitoneal injection of 150 mg/b.w. CP. We established that in the doses applied PTXF had no statistically significant influence on the number of leukocytes and erythrocytes determined in the left ventricular blood of rats receiving CP, while the number of white cells from animals given PTXF only was higher than in controls. There was a smaller decrease in the number of platelets (p < 0.05) and smaller reduction in fibrinogen level (p < 0.01) in the serum of PTXF-CP animals than in the CP group. The results obtained suggest a protective effect of PTXF on CP induced changes, which have been evidenced in some of the parameters examined. Ultrastructural examinations found the lungs to be the organ of extramedullary thrombocytopoiesis in CP-treated animals and revealed that platelet accumulation in the system of lung capillaries was a potential cause of the decrease observed in the number of blood platelets following CP administration.
  • M Sobaniec-Lotowska · W Sobaniec · M Sulkowska · S Sulkowski · W Kułak
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    ABSTRACT: An incidence and morphology of the CNS congenital malformations in newborn babies and infants were analysed in the consecutive autopsies carried out in 1986-1990, i.e. following Tscharnobyl disaster. The obtained results were compared to those seen in the two earlier periods (1976-1980 and 1981-1986). In 1986-1990, a percentage of autopsies showing congenital CNS malformations increased approximately by two-fold (15%). The highest percentage of such malformations in specific years of the analysed period was noted in 1990 (20%). Central nervous system malformations were more frequent in female sex (57%) than in male sex (43%). In 64% of cases newborn babies were affected. A percentage of CNS malformations coexisting with other congenital malformations increased to 40% in the analysed period of time (from 29.4% in 1976-1985). Meningomyelocele (41%), congenital hydrocephalus (21.5%), multiple anomalies in brain (14%), and anencephaly (12.6%) constituted the most frequent group of CNS malformations. In 1986-1990, an incidence of meningomyelocele increased by more than two-fold (if this anomaly coexisted with hydrocephaly, an increase in the incidence exceeded three-fold), and hydrocephaly as well as an increase in the incidence of anencephaly by 1.5 times in comparison with 1976-1985.
    Polski merkuriusz lekarski: organ Polskiego Towarzystwa Lekarskiego 12/1996; 1(5):334-6.
  • M Sobaniec-Lotowska · M Barwijuk · M Baltaziak · J Dziecioł · S Sulkowski · W Debek · H Ostapiuk
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    ABSTRACT: The 19,094 autopsy examinations carried out between 1976-1990 revealed 698 (3.65%) case of cirrhosis, of which 64.6% were men. During the last 5 years the percentage of coexistance of hepatoma (hepatocellular carcinoma) with cirrhosis was higher 5-year periods (5.8%; 5.4%). Moreover, the same changing interrelation was observed for other malignancies and cirrhosis-higher (15%) in the last period than in the proceeding years (11.1%; 11.3%). The severity of atherosclerotic changes and coexistance of peptic ulcers, gall bladder disease and productive pulmonary tuberculosis in cirrhotic patients were also assessed. Finally the direct causes of these patients' death were discussed.
    Polski merkuriusz lekarski: organ Polskiego Towarzystwa Lekarskiego 10/1996; 1(3):187-9.
  • S Sulkowski · B Musiatowicz · M Sulkowska · M Sobaniec-Lotowska · J Dziechiol · M Sulik · B Szynaka
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    ABSTRACT: A morphometrical analysis of changes in the heart of rats with experimental lung emphysema due to single intratracheal administration of papain solution has been done. Special attention has been paid to quantitative changes of capillaries and to the cardiomyocyte diameter in respective regions of the myocardium after one, three and six months following the administration of papain. A histological and morphometrical analysis of changes in the lungs has also been carried out and partial pressures of oxygen and carbon dioxide in the arterial blood of the examined animals have been marked. It has been found that a single administration of papain caused emphysematous changes in the lungs, growing more intense until the third month of the experiment. The progression of these changes corresponded to a capillary density increase in the right ventricle, interventricular septum and in the subendocardial layer of the left ventricle. The increase in the number of capillaries was interpreted as the first adaptive stage of cor pulmonale development and explained by the occurrence of angiogenesis. The changes co-existed with a decrease in partial pressure of oxygen in the arterial blood. After six months of the experiment, a reduction in capillary density in the heart regions mentioned above and a simultaneous increase in the cardiomycyte transversal diameter were observed, being the exponents of evident myocardial hypertrophy. Development of ultrastructural changes in the hearts of rats has been analysed too. Simultaneous development of changes in the right ventricle muscle and in the subendocardial layer of the heart left ventricle myocardium was observed. The role of hypoxia as a one of essential factors responsible for the observed morphologic changes has been discussed.
    Experimental and Toxicologic Pathology 02/1996; 48(1):19-28. DOI:10.1016/S0940-2993(96)80087-X · 1.86 Impact Factor
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    ABSTRACT: The effect of sodium salt of 2,4-dichlorophenoxyacetic acid, being an active component of herbicide "PIELIK", upon the development of Guerin carcinoma implanted in male Wistar rats, was studied. 192 animals were divided in to 6 equal groups: I-animals which obtained physiological salt solution; II-rats exposed to the herbicide in postlactational period; III-animals with Guerin carcinoma, non exposed to the herbicide; IV- rats exposed to the herbicide in postlactational period+Guerin carcinoma; V-animals exposed to the herbicide from prenatal period to the end of an experiment, without Guerin carcinoma; VI-the same as in V group, but with Guerin carcinoma. The effect of the herbicide on tumor growth dynamism (diameters and mass), degree of tumour malignancy (metastases to lymph nodes), animals survival time and morfological changes in the primary tumour and in metastases was evaluated. Basing of the results obtained, it was stated that this herbicide accelerates the development of Guerin carcinoma and reduces the survival time in the rats exposed to it in the prenatal and postnatal period. However, it does not significantly influence the growth of the carcinoma in the rats exposed only in the postlactational period.
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    ABSTRACT: The cell-cell interaction between fibroblasts and alveolar macrophages was examined using a co-culture system. Alveolar macrophages (AM) were harvested from the bronchoalveolar lavages (BAL) of rats with papain induced lung emphysema. The BCG-vaccine was applied as a macrophage mobilizing and activating agent. The morphological examinations carried out in scanning electron microscope (SEM) as well as the evaluation of the uptake of 3H-thymidine did not show any significant differences between respective co-cultures of fibroblasts and AM isolated both from the lungs of control and experimental animals (treated with BCG or papain, and BCG+papain). However, significant growth were noted in 3H-thymidine uptake between fibroblast cultures done with or without cells isolated from the lungs. The results obtained suggest that AM can promote fibroblast proliferation during the progression of experimental lung emphysema.
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    ABSTRACT: Morphological (in light and transmission electron microscope) as well as morphometrical analysis of the lungs was performed on experimental, papain-induced lung emphysema. Development of emphysematous changes was studied seven days after a single intratracheal instillation of papain solution. The effect of alveolar macrophages (AM) activation by BCG-vaccine on changes in pulmonary tissue was analyzed. In the rats given BCG the number of AM increased and demonstrated enhanced activity. Increase in reticulin fibre density in places of AM cumulation, particularly in BCG+papain-treated rats was observed. The lungs of animals treated with BCG+papain showed enhancing of emphysema comparing with the papain-treated rats. Development of emphysematous changes, especially in BCG+papain-treated rats coexisted with cumulation of activated alveolar macrophages and collagen fibres as well as type II alveolar epithelial cells proliferation. Our data support the inflammatory-repair hypothesis of emphysema pathogenesis and indicate that AM regulate collagen production in the lung. Type II alveolar epithelial cells seem be important in lung injury and repair.
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    ABSTRACT: Retrospective analysis is presented for the diagnoses basing on brushing cytology and biopsy histology in 362 patients selected from the total number of 8600 cytologic examinations of gastric mucosa performed in this Department in the years 1984-1992. Endoscopy suggested neoplasma in 58 cases, peptic ulcer in 146 and gastritis in 158 cases. In 64 cytologic specimens neoplastic cells were found (17.7%). Histologic evaluation, in addition to brushing cytology, was possible in 316 cases; in 186 on gastroscopy, in 82 in surgical specimens, in 48 on postmortem. In one case only of positive brushing cytology the diagnosis of cancer was confirmed (1.7%). In 16 cases (8.2%) the brushing cytology is stressed, especially in monitoring subjects of high risk.
    Polskie archiwum medycyny wewnȩtrznej 05/1994; 91(4):267-71. · 2.12 Impact Factor
  • W Sobaniec · M Sobaniec-Lotowska
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    ABSTRACT: Effects of sodium valproate (VPA) and the omega-oxidation metabolites (5-OH-VPA and 4-OH-VPA) on the electroconvulsions in rats were analyzed. The drug (100 and 200 mg/kg/day) revealed marked dose-related anticonvulsant effect which manifested themselves by shortening the convulsions time and their intensity. The anticonvulsant effect of the omega-oxidation (50, 100 and 200 mg/kg/day) were weaker compared with the parent drugs.
    Materia medica Polona. Polish journal of medicine and pharmacy 01/1994; 26(1):29-32.
  • S Sulkowski · M Sulkowska · M Sobaniec-Lotowska · J Dziecioł · B Szynaka · B Musiatowicz
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    ABSTRACT: An analysis was done of 1084 autopsies of children and adolescents up to 18 years of age carried out in the years 1986-1990. In the analysed period 631 cases were found of pneumonia (P) (58.2% autopsies) which was an increase of about 14% in relation to the years 1976-1985. Pneumonia was most frequently observed in the age range 1-12 months. It was the direct cause of death (primary pneumonia--PP) in 130 cases (11.9% autopsies) while in 501 cases (46.2%) it accompanied other primary diseases (secondary pneumonia--SP). In relation to the years 1976-1985 a decrease was observed of the per cent of autopsies with the diagnosis of PP (from 19.5% to 11.9%), as well as a significant increase of the per cent of SP) from 25.1% to 46.2%).
    Wiadomości lekarskie (Warsaw, Poland: 1960) 05/1993; 46(7-8):263-6.
  • M Sobaniec-Lotowska · W Sobaniec
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    ABSTRACT: Effective doses of sodium valproate (200 mg/kg) applied in rats chronically (1, 3, 6, 9 and 12 months) evoked the first morphological changes in the brain hemispheres after 9 months of drug administration. Structural abnormalities of the brain tissue consisted in disseminated nonspecific neuronal lesions and patchy nerve cell loss, more pronounced in the final phase of the experiment. The neuronal lesions were localized predominantly in the 3rd and 5th layers of the neocortex and in the pyramidal cell layer of the hippocampus. They were accompanied by vascular wall alterations, perivascular tissue damage, as well as by microvacuolar changes and spongy degeneration of the subpial and periventricular regions. Vasogenic character of parenchymal changes is stressed by the authors. The possible influence of liver damage on the development of brain pathology is discussed.
    Neuropatologia polska 02/1993; 31(3-4):149-58.