Mancusi A

Publications (2)0 Total impact

• Source

  Available from: Alfredo Pauciullo

  Article: Mediterranean river buffalo oxytocin-neurophysin I (OXT) gene: structure, promoter analysis and allele detection

  Cosenza G, Pauciullo A, Mancusi A, Nicodemo D, R. Di Palo, Zicarelli L, D Di Berardino, Ramunno L
  [show abstract] [hide abstract]
  ABSTRACT: Oxytocin (OXT) is a very abundant nonapeptide neurohypophysial hormone implicated in several aspects of reproduction, including social, sexual and maternal behaviour, induction of labour and milk ejection. The nucleotide sequence of the whole OXTneurophysin I encoding gene (OXT) in Mediterranean river buffalo was determined, plus 993 nucleotides at the 5’ flanking region. Buffalo oxytocin gene sequence analysis showed two transitions in the promoter region (C→T in position – 966 and G→A in position – 790) and one transversion G→T at the 170th nucleotide of the second exon, responsible for the Arg97→Leu aa substitution which identifies an allele named OXT B. A PCR-RFLP based method for a rapid identification of carriers of these alleles has been developed.
  Italian Journal of Animal Science. 01/2010;
• Source

  Available from: Gianfranco Cosenza

  Article: Water buffalo kappa-casein gene sequence

  Masina P, Rando A, P. Di Gregorio, Cosenza G, Mancusi A
  [show abstract] [hide abstract]
  ABSTRACT: The aim of the present work was to determine the nucleotide sequence of the water buffalo CSN3 gene (κ-casein). Two overlapping clones from a genomic water buffalo library were sequenced. The sequence comprises the five exons, the relative introns, 1057 nt at the 5’ end of the gene and 476 nt downstream the polyadenylation site. In order to identify polymorphisms responsible for amino acid differences, all the five exons from 10 genetically unrelated water buffaloes were sequenced. The comparison of the obtained sequences confirmed the two single nucleotide polymorphisms already reported in literature at the fourth exon: T versus C at codon 135 (IleATC versus ThrACC) and the silent mutation T versus C at codon 136. The comparison of the promoter sequences of two animals homozygous for 135Thr and 135Ile respectively, evidenced 3 single nucleotide polymorphisms that could alter the expression of the gene.
  Italian Journal of Animal Science. 01/2010;