Publications (3)1.43 Total impact
Article: Differential toxicity of Mn2+ and Mn3+ to rat liver tissues: oxidative damage, membrane fluidity and histopathological changes.[show abstract] [hide abstract]
ABSTRACT: Toxicity due to overexposure to manganese (Mn) is becoming increasingly prevalent. Mn-induced neurodegenerative toxicity has been demonstrated, but little is known concerning the adverse effects of the element on the liver. Under physiological conditions, manganese primarily exists as divalent manganese (Mn(2+)) and trivalent manganese (Mn(3+)). The present study was designed to evaluate and compare the effects of Mn(2+) and Mn(3+) on oxidative hepatic damage, membrane fluidity and histopathological changes in rats. Rats exposed to Mn(2+) or Mn(3+) (2.0mg Mn/kg body weight) showed significant inhibition of superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity, as well as decreased levels of glutathione (GSH) and increased levels of malondialdehyde (MDA) in liver tissues. We also showed a significant inhibition of SOD activity and increased MDA levels in hepatocyte nuclei. We also observed reduced Na(+),K(+)-ATPase activity, increased MDA levels and decreased plasma membrane fluidity, which was accompanied by an increase of fluorescence anisotropy (r) values, in hepatic plasma membranes. In addition, Mn(2+) and Mn(3+) both caused histopathological changes, such as mononuclear cell infiltration, congestion, enlargement of the veins and sinusoids, hepatocellular damage, necrotic changes, mitochondrial hyperplasia, swelling and vacuolization, as determined by light and electron microscopy. Taken together, these data suggest that both Mn(2+) and Mn(3+) inhibit the normal physiological functioning of the liver. Under the experimental conditions used, the adverse effects of Mn(2+) were more severe than those of Mn(3+).Experimental and toxicologic pathology: official journal of the Gesellschaft fur Toxikologische Pathologie 03/2012; 64(3):197-203. · 1.43 Impact Factor
Article: Effects of lanthanum, cerium, and neodymium on the nuclei and mitochondria of hepatocytes: accumulation and oxidative damage.[show abstract] [hide abstract]
ABSTRACT: The aim of this study was to investigate the contents of lanthanum (La), cerium (Ce), and neodymium (Nd) that accumulate in nuclei and mitochondria isolated from the liver and their corresponding potential oxidative damage effects on nuclei and mitochondria. Five-week-old male imprinting control region (ICR) mice were exposed to chlorides of La, Ce, or Nd by oral gavage with one of three doses: 10, 20, or 40 mg/kgBW/day for 6 weeks. The concentrations of administered elements in hepatocyte nuclei and mitochondria were determined with inductively coupled plasma-mass (ICP-MS) spectrometry. The accumulation of La, Ce, and Nd in hepatocyte nuclei and mitochondria gradually increased in a dose-dependent manner with exposure to the elements, although the concentrations of La, Ce, and Nd in hepatocyte mitochondria were lower than those in their counterpart nuclei. In hepatocyte nuclei, superoxide dismutase (SOD) and catalase (CAT) activities decreased, whereas glutathione peroxidase (GPx) activity, glutathione (GSH) and malondialdehyde (MDA) levels increased. In hepatocyte mitochondria, SOD, CAT, and GPx activities and GSH levels were significantly decreased, and MDA levels were significantly increased. These results suggest that La, Ce, and Nd presumably enter hepatocytes and mainly accumulate in the nuclei and induce oxidative damage in hepatic nuclei and mitochondria.Environmental toxicology and pharmacology. 01/2011; 31(1):25-32.
Article: Effect of high water temperature on mortality, immune response and viral replication of WSSV-infected Marsupenaeus japonicus juveniles and adults[show abstract] [hide abstract]
ABSTRACT: This study evaluated the effect of high water temperature on juvenile mortality and adult immune response and viral replication of white spot syndrome virus (WSSV) infected Kuruma shrimp Marsupenaeus japonicus under standardized conditions. Two methods, oral administration for juveniles and intramuscular injection for adults, were used to infect M. japonicus at two water temperature levels (27 ± 0.5 and 31 ± 0.5 °C). Mortality of WSSV-infected juveniles was largely reduced at 31 °C compared to 27 °C. The total haemocyte count (THC) and phenoloxidase (PO) activity of M. japonicus adults in control treatment (WSSV-free) had no significant difference at 27 and 31 °C (p > 0.05). The THC and PO activity of WSSV high temperature treatment were significantly higher (p < 0.05) than those of WSSV ambient treatment over 24–72 h and 12–72 h post injection, respectively. Real time PCR showed that viral load of WSSV ambient treatment was significantly higher (p < 0.05) than that of WSSV high temperature treatment over 24–72 h post infection. This study confirmed that high water temperature prevented the onset of disease and significantly reduced mortality of WSSV-infected shrimp, and demonstrated that high water temperature inhibited the replication of WSSV instead of increasing the activity of the host immune response. These results provide practical applications to control WSSV in shrimp farming.Aquaculture.