M.A. Versnel

Erasmus MC, Rotterdam, South Holland, Netherlands

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Publications (3)18.54 Total impact

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    ABSTRACT: To identify interleukin (IL)-7Rα expression in the labial salivary gland (LSG) of patients with primary Sjögren's syndrome (pSS) and non-Sjögren's syndrome sicca (nSS-sicca) and to study its correlation with glandular inflammation and IL-7 expression. The presence of infiltrating immune cells and IL-7Rα cells in inflamed LSG of patients with pSS (n=12) and nSS-sicca controls (n=7) was studied by immunohistochemistry and fluorescence activated cell sorting analysis upon tissue digestion (n=15 and n=13, respectively). Additionally, the correlations of IL-7Rα cells with hallmark disease parameters of pSS, major infiltrating inflammatory cells and IL-7 were assessed. In the LSG of patients with pSS increased numbers of IL-7Rα cells were found as compared with nSS-sicca patients. IL7Rα cells strongly correlated with the lymphocytic focus score, IL-7 expression, the decrease in percentage of IgA plasma cells and numbers of CD3 T cells, CD20 B cells, and CD1a and CD208 myeloid dendritic cells. Analysis of isolated cells from the LSG demonstrated strongly increased percentages of IL-7Rα CD3 T cells in pSS as compared with nSS, showing abundant IL-7Rα expression on both CD4 and CD8 T cells. Other CD45 leucocytes and CD45- tissue cells scarcely expressed IL-7Rα. Percentages of IL-7Rα T cells also significantly correlated with glandular inflammation. This study shows the presence of increased IL-7Rα T cells in the LSG of patients with pSS and their association with the severity of sialadenitis, disease parameters and IL-7 expression. Considering the immunostimulatory ability of IL-7Rα T cells and IL-7, this suggests that IL-7(R)-dependent T cell-driven immune activation plays an important role in inflammation in pSS.
    Annals of the rheumatic diseases 02/2012; 71(6):1027-33. DOI:10.1136/annrheumdis-2011-200744 · 9.27 Impact Factor
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    ABSTRACT: In patients with pSS local T cell-driven inflammation contributes to destruction of exocrine glands associated with clinical symptoms of dryness. Recently the authors documented increased interleukin (IL)-7 in labial salivary glands (LSG) of pSS patients that was capable to induce Th1 and Th17 activity and proinflammatory cytokine secretion. IL-7 mediates its effects by signaling through the high affinity IL-7Rα subunit and γc chain. The authors and others have shown that IL-7R+ CD4 T cells that strongly proliferate upon TCR activation, while IL-7R− CD4 T cells are anergic and can be regulatory of nature. This suggests that IL-7R+ T cells contribute to the increased inflammatory response in LSG of pSS patients, especially in the presence of increased local IL-7 expression.To identify IL-7R expression in the labial salivary gland and to examine the phenotypical characteristics of IL-7R+ T cells between pSS and non-Sjögren's syndrome sicca (nSS) patients. The presence of infiltrating immune cells and IL-7R+ cells in inflamed salivary glands of pSS patients (n=14) and non-inflamed LSG of nSS patients (n=7) was studied by immunohistochemistry and FACS analysis upon tissue digestion.In the LSG of pSS patients significantly increased numbers of IL-7R+ cells were found as compared to nSS (pSS vs nSS; 244.3±40.7 vs 12.3±4.6 cells/mm2). IL7R+ T cells were found throughout the tissue but mainly in the CD3-rich lymphocytic areas. IL7R+ T cells significantly (all p
    Annals of the Rheumatic Diseases 02/2011; 70(2). DOI:10.1136/ard.2010.148981.22 · 9.27 Impact Factor