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ABSTRACT: After intraperitoneal administration of 15N-labeled serine, ethanolamine and choline to young mice showing active myelination, the incorporation of 15N-atom into N-bases of phospholipids and long chain bases of sphingolipids in brain tissue was particularly investigated in comparison with those in visceral tissues of adult mouse. After intraperitoneal injection of DL-[15N]-serine into mice, it was found that the 15N-atom was obviously incorporated into phosphatidylserine, phosphatidylethanolamine, ethanolamine-plasmalogen and long chain bases of cerebroside, sphingomyelin and gangliosides, but not significantly into phosphatidylcholine. The apparent maximum rates of the incorporation were also calculated. After administration of [15N]-ethanolamine, the 15N-atom was predominantly incorporated into phosphatidylethanolamine and next into ethanolamine-plasmalogen, but also not significantly into phosphatidylcholine. After administration of [15N]-choline chloride, the 15N-atom was incorporated into phosphatidylcholine and sphingomyelin, but not into other phospholipids and sphingolipids. These results suggested that serine, ethanolamine and choline are available for the biosynthesis of the N-bases of phospholipids in the brain tissue of young mouse and that serine is also utilized for the biosynthesis of long chain bases of sphingolipids. However, neither biosynthesis of choline from ethanolamine nor of phosphatidylcholine from phosphatidylethanolamine by methylation seemed to take place even in the brain tissue of young mouse, while the decarboxylation of phosphatidylserine into phosphatidylethanolamine was obviously observed. The decarboxylation rate of phosphatidylserine into phosphatidylethanolamine in the brain tissue of young mouse seemed to be approximately at the same level with the other tissues like liver, lung spleen and kidney of adult mouse.
The Japanese journal of experimental medicine 09/1980; 50(4):267-73.
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ABSTRACT: The role of hydrophobic moiety in determining the immunologic properties of glycolipid haptens was studied with the derivatives of Forssman glycolipid modified by ozonolytic cleavage of double bonds in hydrocarbon chains. The immunogenicity was not affected by this modification; comparable antibody responses were elicited in rabbits immunized with intact or modified Forssman glycolipid, regardless of its different hydrophobicity, in complete Freunds adjuvant. Modified hydrophobic portions did not affect the class and specificity of antibodies produced. On the other hand, immunologic reactivity was strongly influenced by hydrophobic moiety; the ability to form immune precipitates with antibodies decreased as the hydrophobicity of glycolipid decreased. The reactivity in complement fixation and hemolysis inhibition tests also showed parallel decreases, though the addition of auxiliary lipids greatly reduced these differences in reactivity. The results indicate the different influences of hydrophobic moiety of glycolipids on their immunogenicity and immunologic reactivity in vitro.
The Japanese journal of experimental medicine 03/1979; 49(1):1-8.
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ABSTRACT: Antibodies against a glycolipid of guinea pig erythrocyte membranes were prepared in rabbits by immunization with guinea pig erythrocyte stroma or the purified glycolipid, gangliotriaosylceramide. The antibodies agglutinated guinea pig erythrocytes. The specificity of antibodies could be revealed by several immunochemical methods, including inhibition of hemagglutination, immunodiffusion, agglutination of liposomes, and complement fixation. The antibodies were specific for gangliotriaosylceramide.
Journal of Biochemistry 05/1978; 83(4):1199-201. · 2.37 Impact Factor
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ABSTRACT: Several neutral glycolipids and gangliosides were isolated from bovine erythrocyte stroma. Their structures were determined by partial acid hydrolysis, methylation analysis, periodate oxidation and CrO3 oxidation. Two major neutral glycolipids were characterized as lactosylceramide and galactosyl(alpha1--3)galactosyl(beta1--4)N-acetylglucosaminyl(beta1--3)galactosyl(beta1--4)glucosyl(beta1--1)ceramide. Two major gangliosides were N-glycolylneuraminosyl(2--3)galactosyl(beta1--4)glucosyl(beta1--1)ceramide and N-glycolylneuraminosyl(2--3)galactosyl(beta1--4)N-acetylglucosaminyl(beta1--3)galactosyl(beta1--4)glucosyl(beta1--1)ceramide. Minor glycolipids were glucosyl- and galactosylceramide, glucosamine-containing tri- and tetraglycosylceramide, glucosamine-containing disialosylhexaglycosylceramide, and gangliosides containing N-acetylneuraminic acid. The ceramide moiety of each glycolipid contained perdominantly d18:1 sphingosine, and normal fatty acids of C16:0, C22:0, C24:0, and C24:1.
Journal of Biochemistry 03/1978; 83(2):463-71. · 2.37 Impact Factor
