M J Koehler

Publications (3)1.71 Total impact

• Article: Keratinocyte morphology of human skin evaluated by in vivo multiphoton laser tomography.

  M J Koehler, S Zimmermann, S Springer, P Elsner, K König, M Kaatz
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  ABSTRACT: Multiphoton tomography (MPT) is a novel non-invasive imaging method in dermatology allowing the depiction of the epidermis with sub-cellular resolution. Here, we present a descriptive characterization of unaffected human epidermis, morphometric data on human keratinocytes and some epidermal parameters in vivo and a morphological characterization of keratinocyte changes in actinic keratoses. In a clinical setting, 57 volunteers of different age groups were examined using MPT. The morphological appearance of keratinocytes showed polygonal cells in the horny layer, a granular cytoplasm in the stratum granulosum, smaller prickle cells in the stratum spinosum and hyperpigmented small round basal cells. Actinic keratoses presented remarkable differences including widened inter-cellular spaces, heterogeneity in cellular fluorescence and shape as well as an increased ratio of nuclear to cellular size. Finally, the thickness of the epidermis was significantly increased in actinic keratoses compared with the control. In vivo MPT provides high-resolution images allowing the identification and quantification of cellular morphometric parameters. First observations of morphology and morphometry of actinic keratoses are reported.
  Skin Research and Technology 03/2011; 17(4):479-86. · 1.71 Impact Factor
• Article: Actinic damage and its treatments.

  M J Koehler, P Elsner, M Kaatz
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  ABSTRACT: Over the last decades, there has been a dramatic increase of skin tumour incidence that results from actinic damage. This increase is mainly due to the current demographic development, but also due to a change in spare time habits. Thus, the treatment and prevention of light induced skin tumours is gaining interest, but also the treatment of photoaged skin. This review will focus on 1) the basic principles of actinic damage including carcinogenesis; 2) treatment options for photoaged skin and 3) an overview of current therapy concepts of light induced skin tumours.
  Giornale Italiano di Dermatologia e Venereologia 04/2010; 145(2):175-85.
• Source

  Available from: msu.edu

  Article: RegRand: statistical software for the multiple-baseline design.

  M J Koehler, J R Levin
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  ABSTRACT: RegRand (Version 1.0) is Macintosh-based software that enables a researcher to conduct a non-parametric statistical analysis of the data from Koehler and Levin's (1998) recently described regulated randomization single-case multiple-baseline design. Regulated randomization design and analysis principles are reviewed in relation to an educational research application and a step-by-step illustration of them in relation to the RegRand program is presented.
  Behavior research methods, instruments, & computers: a journal of the Psychonomic Society, Inc 06/2000; 32(2):367-71.