M E Gonzalez

Complutense University of Madrid, Madrid, Madrid, Spain

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Publications (5)6.48 Total impact

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    M C Rojo, M E Gonzalez
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    ABSTRACT: Apoptosis is a form of naturally occurring cell death during development and it is characterised by extensive DNA fragmentation. Apoptosis is easily detected in the gill epithelium of brown trout embryos in ultrathin sections (Rojo et al. 1997). Here we provide the first biochemical evidence for apoptosis in the gill epithelium of brown trout embryos, using in situ end-labelling of DNA breaks (Gavrieli et al. 1992). Embryos at d 57 of development as well as those at hatching, were processed to analyse the distribution of apoptotic cells in the gills. The extent of apoptosis revealed by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labelling method technique is considerably greater than apoptosis detected by nuclear morphology. This method revealed that apoptosis was frequent at hatching, although it was also present during embryonic development. The presence and distribution of stained nuclei were different depending on the developmental stage. In embryos of 57 d, apoptotic flattened nuclei were dispersed in the gill epithelium, whereas at hatching, they were mainly grouped in the tips of the filaments and in the gill arches. TUNEL also revealed a distinct pattern of nuclear staining: at hatching, the intense staining covered the entire cell, but in embryos it was restricted to the nucleus. These results show the functional relevance of apoptosis at hatching, when apoptosis seems to be the unique process by which cell numbers in the gill epithelium are adjusted, in order to prepare for the new extrinsic conditions affecting the free-living life of alevins.
    Journal of Anatomy 11/1998; 193 ( Pt 3):391-8. · 2.36 Impact Factor
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    M C Rojo, M J Blánquez, M E González
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    ABSTRACT: A histochemical study of the branchial area of brown trout embryos from 35 to 71 d of incubation is reported. A battery of 6 different horseradish peroxidase-labelled lectins, the PAS reaction and Alcian blue staining were used to study the distribution of carbohydrate residues in glycoconjugates along the pharyngeal and branchial epithelia. Con A and WGA reacted at every site of the branchial region thus showing the ubiquitous presence of alpha-D-mannose and N-acetyl-D-glucosamine. WGA, DBA and SBA were good markers for the hatching gland cells (HGCs) and mucous cells. Other lectins, such as PNA and UEA I, reacted only for a short time at some sites during the considered period of incubation. From 35 d until posthatching stages, a manifest strong reaction was noted both in the dorsal epithelium of branchial arches and the HGCs as shown by SBA reactivity. This may be significant with regard to the controversial origin of HGCs, which is thought to be endodermal.
    Journal of Anatomy 01/1997; 189 ( Pt 3):609-21. · 2.36 Impact Factor
  • J Illanes, M J Blanquez, M E Gonzalez, C Rojo
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    ABSTRACT: The teratogenic effect of alcohol on chick embryos has been confirmed by many investigators. However, how this occurs is unknown. The aim of this study was to establish a teratogenic pattern of alcohol effects, on the first stages of development in avians. Fertilized eggs were infused through the air space of the shell on day 0, with ethanol in concentrations of 20%, 40% and 60%. The control group was infused with 0.1 ml of NaCl at 0.9%. At a second stage, the eggs were treated on the 4th day of incubation, using the same method. In both groups the eggs were removed on the 11th day of incubation. The teratological manifestations that appeared more frequently were evisceration, haemorrhagic embryos, oedema, cranial deformities, lack of eyes, and umbilical hernia, showing every embryo a clear reduction in size and body weight.
    Anantomia Histologia Embryologia 01/1996; 24(4):217-21. · 0.88 Impact Factor
  • M E González, M J Blánquez, C Rojo, A J Puerta
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    ABSTRACT: The development of the cephalic region of rainbow trout in the 24th, 30th and 36th stages, corresponding to the table of development by Vernier (1969), was studied using light microscopy and SEM. The 24th stage shows a voluminous cephalic region as a principal feature. At this time, most components of this region have started their differentiation. The 30th stage can be considered as an intermediary phase in the embryonic formation, and it is characterized by the high degree of differentiation between the nervous system and the sense organs. In the 36th stage, the basic final shape of the embryo has been reached. The results of this study show that the differentiation of gill lamellae and the opening of the mouth takes place later than that moment described by other authors. This suggests that development between 30th and 36th stages is a critical survival period for rainbow trout, in which respiratory and digestive functions start.
    Anantomia Histologia Embryologia 01/1996; 24(4):257-63. · 0.88 Impact Factor
  • J. Illanes, M. J. Blanquez, M. E. Gonzalez, C. Rojo
    Anatomia Histologia Embryologia - ANAT HISTOL EMBRYOL. 01/1995; 24(4):217-221.

Publication Stats

12 Citations
6.48 Total Impact Points


  • 1996–1998
    • Complutense University of Madrid
      • Departamento de Anatomía y Anatomía Patológica Comparada (Anatomía y Embriología)
      Madrid, Madrid, Spain