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Kaifan Yu,
Gang Shu,
Fangfang Yuan,
Xiaotong Zhu,
Ping Gao,
Songbo Wang,
Lina Wang,
Qianyun Xi,
Shouquan Zhang,
Yongliang Zhang,
Yan Li,
Tongshan Wu, Li Yuan,
Qingyan Jiang
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ABSTRACT: Fat and lean pig breeds show obvious differences in meat quality characteristics including the fatty acid composition of muscle. However, the molecular mechanism underlying these phenotypes differences remains unknown. This study compared meat quality traits between Lantang (a Chinese indigenous breed) and Landrace (a typical lean breed). The Lantang pigs showed higher L* values and intramuscular fat content, lower pH(45min), pH(24h) and shear force in longissimus dorsi (LD) muscle than Landrace (P < 0.05). Fatty acid analysis demonstrated the lower monounsaturated fatty acids (MUFA) and higher polyunsaturated fatty acids (PUFA) percentage in Lantang LD than that in Landrace LD (P < 0.05). To further identify candidate genes for fatty acid composition, the transcriptome of LD muscle from the two breeds were measured by microarrays. There were 586 transcripts differentially expressed, of which 267 transcripts were highly expressed in Lantang pigs. After the validation by real-time quantitative PCR, 13 genes were determined as candidate genes for fatty acid composition of muscle, including Stearoyl-CoA desaturase (SCD). Then, a SCD over-expression plasmid was transfected into C2C12 cells to reveal the effect of SCD on the fatty acid composition in vitro. The results showed that SCD over-expression significantly increased PUFA proportion, while reduced that of saturated fatty acids (SFA) in C2C12 cells (P < 0.05). In summary, this study compared the differences of fatty acid composition and transcriptome in two breeds differing in meat quality, and further identified the novel role of SCD in the regulation of PUFA deposition.
International journal of biological sciences 01/2013; 9(1):108-18. · 2.70 Impact Factor
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ABSTRACT: Here, for the first time, we evaluate the hypothesis that the proliferative abilities of satellite cells (SCs) isolated from Lantang (indigenous Chinese pigs) and Landrace pigs, which differ in muscle characteristics, are different. SCs were isolated from the longissimus dorsi muscle of neonatal Lantang and Landrace pigs. Proliferative ability was estimated by the count and proliferative activity of viable cells using a hemocytometer and MTT assay at different time points after seeding, respectively. Cell cycle information was detected by flow cytometry. Results showed that there was a greater (P
PLoS ONE 03/2012; 7(3). · 4.09 Impact Factor
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Hong-Yi Li,
Qian-Yun Xi,
Yuan-Yan Xiong,
Xiao-Long Liu,
Xiao Cheng,
Gang Shu,
Song-Bo Wang,
Li-Na Wang,
Ping Gao,
Xiao-Tong Zhu,
Qing-Yan Jiang, Li Yuan,
Yong-Liang Zhang
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ABSTRACT: MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that negatively regulate gene expression at the post-transcriptional level. Although an increasing number of porcine miRNAs recently have been identified, research has yet to identify the full repertoire of miRNAs in pig skeletal and adipose tissues and their differences between breeds. We extracted small RNA from skeletal muscle and adipose tissues of Landrace and Lantang pigs, and the expression of a total of 184 known porcine miRNAs (113 from Solexa sequencing and 171 from miRNA chip hybridization) as well as 521 novel miRNA candidates was detected. Moreover, 20 miRNAs were selected randomly from the 184 miRNAs and analysed by quantitative real-time PCR to confirm the aforementioned results. In the skeletal muscle tissues, 21 miRNAs were up-regulated in Lantang and another 33 were highly expressed in Landrace pigs. In the adipose tissues, 25 miRNAs were down-regulated in Lantang and another 23 were lowly expressed in Landrace pigs. miRNA divergence between tissues was also detected in this study. Ten miRNAs were highly expressed in the skeletal muscle tissue in comparison with adipose tissue, and another 10 miRNAs exhibited the opposite expression profile. To investigate the regulatory mechanism of the miRNAs in muscle and adipose tissues, the 10 miRNAs with the most divergent expression profiles were functionally categorized using the Kyoto Encyclopedia of Genes and Genomes database. Most of the miRNAs strongly corresponded to myogenesis and adipogenesis processes. In addition, 84 of the 521 miRNA candidates were potentially porcine-specific miRNAs. This study adds new valuable information to comparative miRNA profiles of skeletal muscle and adipose tissues in porcine species. The great diversity of miRNA composition and expression levels both between breeds and between tissues suggests that a complex regulatory network exists in porcine subcutaneous fat development.
Animal Genetics 02/2012; 43(6):704-13. · 2.40 Impact Factor
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Hongyi Li,
Qianyun Xi,
Yuanyan Xiong,
Xiao Cheng,
Qien Qi,
Lin Yang,
Gang Shu,
Songbo Wang,
Lina Wang,
Ping Gao,
Xiaotong Zhu,
Qingyan Jiang,
Yongliang Zhang, Li Yuan
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ABSTRACT: MicroRNAs (miRNAs) are an abundant class of small RNAs that regulate expressions of most genes. miRNAs play important roles in the pituitary, the "master" endocrine organ.However, we still don't know which role miRNAs play in the development of pituitary tissue or how much they contribute to the pituitary function. By applying a combination of microarray analysis and Solexa sequencing, we detected a total of 450 miRNAs in the porcine pituitary. Verification with RT-PCR showed a high degree of confidence for the obtained data. According to the current miRBase release17.0, the detected miRNAs included 169 known porcine miRNAs, 163 conserved miRNAs not yet identified in the pig, and 12 potentially new miRNAs not yet identified in any species, three of which were revealed using Northern blot. The pituitary might contain about 80.17% miRNA types belonging to the animal. Analysis of 10 highly expressed miRNAs with the Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the enriched miRNAs were involved not only in the development of the organ but also in a variety of inter-cell and inner cell processes or pathways that are involved in the function of the organ. We have revealed the existence of a large number of porcine miRNAs as well as some potentially new miRNAs and established for the first time a comprehensive miRNA expression profile of the pituitary. The pituitary gland contains unexpectedly many miRNA types and miRNA actions are involved in important processes for both the development and function of the organ.
PLoS ONE 01/2011; 6(9):e24883. · 4.09 Impact Factor
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Guixuan Zhou,
Songbo Wang,
Zhonggang Wang,
Xiaotong Zhu,
Gang Shu,
Weiyi Liao,
Kaifan Yu,
Ping Gao,
Qianyun Xi,
Xiuqi Wang,
Yongliang Zhang, Li Yuan,
Qingyan Jiang
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ABSTRACT: The objective of this study was to compare the differences of gene expression profiles between intramuscular and subcutaneous adipocytes originated from the isolated preadipocytes in vitro. Cytosolic triglyceride determination indicated that subcutaneous adipocytes accumulated more lipid than intramuscular adipocytes did at the late stage of differentiation. Microarray assay revealed that 172 probes representing 133 genes were differentially expressed, among which 46 genes were highly expressed in intramuscular adipocytes and the other 87 genes were highly expressed in subcutaneous adipocytes. Real-time PCR confirmed that genes related to lipid metabolism, such as LPL, FABP4, FABP5 and OSBPL10, were predominantly expressed in subcutaneous adipocytes, whereas BMP4 and BMP7 were highly expressed in intramuscular adipocytes. The results indicated that the accumulation of lipid mass in subcutaneous adipocytes might be due to the highly expressed genes related to lipid metabolism, and the high levels of BMP4 and BMP7 in intramuscular adipocytes suggested that BMPs might be involved in the differentiation of intramuscular adipocytes.
Meat Science 10/2010; 86(2):440-50. · 2.28 Impact Factor
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ABSTRACT: To observe the effects of different lengths of needle-retaining time on the therapeutic results of acupuncture treatment for ischemic wind-stroke.
According to the severity of disease, 251 cases of ischemic wind-stroke were randomly divided into 3 groups and treated by acupuncture, with the needle-retaining time lasting 20, 40, and 60 minutes respectively. Observation was mainly focused on the scores of nervous dysfunction and the clinical therapeutic results.
The comparison of the scores of nervous dysfunction in the 3 groups before and after the treatment showed that under Wilcoxon's pair-match symbol rank test, except for horizontal gaze, other indexes all showed significant differences (P < 0.01). In the between-group comparison before and after the treatment, there showed significant or very significant differences in the indexes of myodynamia of the upper limb, myodynamia of hand and the total scores (P < 0.05, P < 0.01). In the between-group comparison by Mann-Whitney rank test of the indexes of myodynamia of upper limb, myodynamia of hand and the total scores, the 20-min. group and the 60-min. group showed significant differences (P < 0.05); and 40-min. group and 60-min. group showed significant differences in myodynamia of upper limb (P < 0.05). In comparison of the therapeutic effects of the 3 groups by Kruskal-wallis rank test, there showed very significant differences (P < 0.01). In the between-group comparison by Mann-Whitney rank test, the 40-min.group and 60-min. group showed significant differences (P = 0.013); while the 20-min. group and 60-min. group showed very significant differences (P = 0.000).
There is a time-effect relationship between the length of needle-retaining time and the therapeutic effects of acupuncture for ischemic wind-stroke.
Journal of Traditional Chinese Medicine 07/2006; 26(2):83-7. · 0.30 Impact Factor
