Publications (34)70.14 Total impact
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Article: Suppression of metastasis of human breast cancer cells by chitosan oligosaccharides.
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ABSTRACT: The present study investigated the antimetastatic property of chitosan oligosaccharides (COS) by evaluating motility, invasion, and the amount and activity of MMP-9 in MDA-MB-231 human breast carcinoma cells. Treatment of MDA-MB-231 cells with increasing concentrations of COS led to a concentration-dependent decrease in cell migration. COS significantly inhibited the invasion of MDA-MB-231 cells through a Matrigel-coated membrane. The treatment of MDA-MB-231 cells with COS reduced the amounts of secreted MMP-9. The activity and amount of MMP-9 protein in MDA-MB-231 cells were decreased by treatment with COS and occurred in a concentration-dependent manner. Our data indicated that COS can serve as a potential novel therapeutic candidate for the treatment of metastatic breast cancer.Journal of Microbiology and Biotechnology 07/2009; 19(6):629-33. · 1.38 Impact Factor -
Article: Chemopreventive effects of polysaccharides extract from Asterina pectinifera on HT-29 human colon adenocarcinoma cells.
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ABSTRACT: We examined the effects of polysaccharides extracted from Asterina pectinifera on the activities of quinone reductase (QR), glutathione S-transferase (GST), ornithine decarboxylase (ODC), cyclooxygenase (COX)-2 and glutathione (GSH) levels in HT-29 human colon adenocarcinoma cells. We found that the polysaccharides extract induced QR activity in a dose-dependent manner over a concentration range of 20 approximately 60 microg/ml and increased GST activity as much as 1.4-fold over controls. GSH levels were increased 1.3- and 1.5-fold with the extract at 40 and 60 microg/ml, respectively. The activity and protein expression of ODC in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced colon cancer cells was inhibited by the extract. The polysaccharides suppressed TPA-induced prostaglandin (PG) production. These data indicate that polysaccharides from A. pectinifera increase phase II detoxification enzyme activity and inhibit ODC and COX-2 activities in HT-29 human colon adenocarcinoma cells. Consequently, this effect may contribute to the protective effect of polysaccharides from A. pectinifera against colon cancer.BMB reports 06/2009; 42(5):277-80. · 1.72 Impact Factor -
Article: Proteomic analysis for inhibitory effect of chitosan oligosaccharides on 3T3-L1 adipocyte differentiation.
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ABSTRACT: In the present study, we performed a differential proteomic analysis using 2-DE combined with MS to clarify the molecular mechanism for the suppressive effect of chitosan oligosaccharides (CO) during differentiation of adipocyte 3T3-L1. Cell differentiation was significantly inhibited by CO at the concentration of 4 mg/mL. Protein mapping of adipocyte homogenates by 2-DE revealed that numerous protein spots were differentially altered in response to CO treatment. Out of 50 identified proteins showing significant alterations, six were up-regulated and 44 were down-regulated by CO treatment in comparison to control mature adipocytes. Among them, most of the proteins are associated with lipid metabolism, cytoskeleton, and redox regulation, in which the levels of farnesyl diphosphate synthetase (FDS), dedicator of cytokinesis 9 (DOCK9), and chloride intracellular channel 1 (CLIC1) were significantly reduced (>two-fold) with CO treatment. These results have not previously been examined in the context of adipogenesis, and thus can be used as novel biomarkers. Taken together with immunoblot analysis, it was concluded that the inhibitory effect of CO on adipocyte differentiation was mediated by C/EBPalpha and PPARgamma pathway through significant downregulations of important adipogenic molecules such as fatty acid binding protein and glucose transporter 4.PROTEOMICS 02/2008; 8(3):569-81. · 4.51 Impact Factor -
Article: Ginkgolide C inhibits platelet aggregation in cAMP- and cGMP-dependent manner by activating MMP-9.
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ABSTRACT: In this report, we investigated the effect of ginkgolide C (GC) from Ginkgo biloba leaves in collagen (10 mug/ml)-stimulated platelet aggregation. It has been known that matrix metalloproteinase-9 (MMP-9) is released from human platelets, and that it significantly inhibited platelet aggregation stimulated by collagen. Zymographic analysis confirmed that pro-MMP-9 (92-kDa) was activated by GC to form an activated MMP-9 (86-kDa) on gelatinolytic activities. And then, GC dose-dependently inhibited platelet aggregation, intracellular Ca(2+) mobilization, and thromboxane A(2) (TXA(2)) formation in collagen-stimulated platelets. In addition, GC significantly increased the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which have an anti-platelet function in both resting and collagen-stimulated platelets. Therefore, we demonstrate that the inhibitory effect of GC on platelet aggregation might be involved into the following pathways. GC may increase intracellular cAMP and cGMP production and MMP-9 activity, inhibit intracellular Ca(2+) mobilization and TXA(2) production, thereby leading to inhibition of platelet aggregation. These results strongly indicate that GC is a potent inhibitor of collagen-stimulated platelet aggregation. It may be a suitable tool for a negative regulator during platelet activation.Biological & Pharmaceutical Bulletin 01/2008; 30(12):2340-4. · 1.66 Impact Factor -
Article: Inhibition of proinflammatory cytokine-induced invasiveness of HT-29 cells by chitosan oligosaccharide.
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ABSTRACT: The effect of chitosan oligosaccharide (COS, 1 kDa <MW<3 kDa) on proinflammatory cytokines-induced nitric oxide (NO) production and invasiveness of human colorectal adenocarcinoma HT-29 cells was investigated. COS (0.1-5mg/ ml) suppressed the NO production induced by proinflammatory cytokines (100 U/ml IFN-gamma, 10 ng/ml IL-1alpha, and 25 ng/ml TNF-alpha) in HT-29 cells. Inducible nitric oxide synthase (iNOS) expression induced by these cytokines was inhibited by COS. COS pretreatment inhibited the invasiveness of cytokines-treated HT-29 cells through Matrigel-coated membrane in a dose-dependent manner. COS also inhibited cytokines-induced matrix metalloproteinase (MMP)-2 activity. This study shows that proinflammatory cytokines induce NO production, iNOS expression, and invasiveness of human colorectal adenocarcinoma HT-29 cells. COS pretreatment inhibited cytokines-mediated NO production, iNOS expression, and invasiveness of HT-29 cells. These results provide sufficient information for the further development of COS as an antitumor metastatic agent for the treatment of colon cancer.Journal of Microbiology and Biotechnology 12/2007; 17(12):2042-5. · 1.38 Impact Factor -
Article: Inhibitory effect of ginkgolide B on platelet aggregation in a cAMP- and cGMP-dependent manner by activated MMP-9.
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ABSTRACT: Extracts from the leaves of the Ginkgo biloba are becoming increasingly popular as a treatment that is claimed to reduce atherosclerosis, coronary artery disease, and thrombosis. In this study, the effect of ginkgolide B (GB) from Ginkgo biloba leaves in collagen (10 microg/ml)- stimulated platelet aggregation was investigated. It has been known that human platelets release matrix metalloproteinase- 9 (MMP-9), and that it significantly inhibited platelet aggregation stimulated by collagen. Zymographic analysis confirmed that pro-MMP-9 (92-kDa) was activated by GB to form an MMP-9 (86-kDa) on gelatinolytic activities. And then, activated MMP-9 by GB dose-dependently inhibited platelet aggregation, intracellular Ca2+ mobilization, and thromboxane A2 (TXA2) formation in collagen-stimulated platelets. Activated MMP-9 by GB directly affects down-regulations of cyclooxygenase-1 (COX-1) or TXA2 synthase in a cell free system. In addition, activated MMP-9 significantly increased the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which have the anti-platelet function in resting and collagen-stimulated platelets. Therefore, we suggest that activated MMP-9 by GB may increase the intracellular cAMP and cGMP production, inhibit the intracellular Ca2+ mobilization and TXA2 production, thereby leading to inhibition of platelet aggregation. These results strongly indicate that activated MMP-9 is a potent inhibitor of collagen-stimulated platelet aggregation. It may act a crucial role as a negative regulator during platelet activation.Journal of biochemistry and molecular biology 10/2007; 40(5):678-83. · 2.02 Impact Factor -
Article: Chemopreventive effect of chitosan oligosaccharide against colon carcinogenesis.
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ABSTRACT: Chitosan oligosaccharide (COS, 3 kDa<MW<5 kDa) was tested for colon cancer chemoprevention by measuring the activities of quinine reductase (QR) and glutathione-S-transferase (GST), glutathione (GSH) levels, ornithine decarboxylase (ODC) activity, and cyclooxygenase (COX)-2 expression in HT-29 cells treated with COS. COS induced QR activity in a dose-dependent manner over a concentration range of 0.1-4.0 mg/ml. GST activity was also induced in HT-29 cells treated with COS. In addition, GSH levels were increased 1.3-, 1.4-, and 1.5-fold with COS at 2, 3, and 4 mg/ml, respectively. ODC activity induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) was inhibited by 33% and 39% with 3 and 4 mg/ml of COS, respectively. COS also inhibited the expression of TPA-induced COX-2 protein in HT-29 cells. These results suggest that COS has colon cancer chemopreventive activity by increasing QR and GST activities and GSH levels and by inhibiting ODC activity and COX-2 expression in vitro.Journal of Microbiology and Biotechnology 09/2007; 17(9):1546-9. · 1.38 Impact Factor -
Article: Effective chemopreventive activity of genistein against human breast cancer cells.
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ABSTRACT: Chemopreventive and cytotoxic effect of genistein against human breast cancer cell lines was investigated. Genistein inhibited cell proliferation in estrogen receptor-positive (MCF-7) and estrogen receptor-negative (MDA-MB-231) human breast carcinoma cell lines. Cytochrome P450 (CYP) 1A1-mediated ethoxyresorufin O-deethylase (EROD)activity was inhibited by genistein in a concentration-dependent manner. Genistein significantly inhibited 12-Otetradecanoylphorbol-13-acetate (TPA)-induced cyclooxygenase-2 activity and protein expression at the concentrations of 10 (p < 0.05), 25 (p < 0.05) and 50 mM (p < 0.01). In addition, ornithine decarboxylase (ODC) activity was reduced to 53.8 % of the control after 6 h treatment with 50 mM genistein in MCF-7 breast cancer cells. These results suggest that genistein could be of therapeutic value in preventing human breast cancer.Journal of biochemistry and molecular biology 08/2006; 39(4):448-51. · 2.02 Impact Factor -
Article: Chemopreventive effect of protein extract of Asterina pectinifera in HT-29 human colon adenocarcinoma cells.
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ABSTRACT: We investigated the effect of protein extract of Asterina pectinifera on the activity of 4 enzymes that may play a role in adenocarcinoma of the colon: quinone reductase (QR), glutathione S-transferase (GST), ornithine decarboxylase (ODC), and cyclooxygenase (COX)-2. QR and GST activity increased in HT-29 human colon adenocarcinoma cells increased that had been exposed to 4 concentrations of the protein extract (80, 160, 200, and 240 microg/mL). Additionally, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity decreased significantly in cells exposed to the extract in concentrations of 160 microg/mL (p<0.05), 200 microg/mL (p<0.005), and 240 microg/mL (p<0.005). TPA-induced COX-2 activity also decreased in cells exposed to extract concentrations of 10, 20, 40, and 60 microg/mL. COX-2 expression was also inhibited in cells exposed to this extract. These results suggest that this protein extract of A pectinifera has chemopreventive activity in HT-29 human colon adenocarcinoma cells, and therefore, may have the potential to function as a chemopreventive agent in human colorectal cancer.Archives of Pharmacal Research 03/2006; 29(3):209-12. · 1.59 Impact Factor -
Article: A novel function of benzyl isothiocyanate in vascular smooth muscle cells: the role of ERK1/2, cell cycle regulation, and matrix metalloproteinase-9.
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ABSTRACT: Dietary isothiocyanates (ITCs) have shown protective effects against certain chemically induced cancers in animal models. These inhibitory effects are associated with reduced levels of extracellular signal-regulated kinase (ERK) 1/2 activity and the arrest of the G(1) cell cycle. Benzyl isothiocyanate (BITC) treatment down-regulates cyclins and CDKs and up-regulates the expression of the CDK inhibitor p21, but up-regulation of p27 or p53 was not detected. Since antiatherogenic effects are not needed for antiproliferation, we determined whether BITC exerted inhibitory effects on matrix metalloproteinase-9 (MMP-9) activity in TNF-alpha-induced vascular smooth muscle cells (VSMCs). BITC inhibited TNF-alpha-induced MMP-9 secretion in VSMC in a dose dependent manner. This inhibition was characterized by the down-regulation of MMP-9, which is transcriptionally regulated at the NF-kappaB site, and the activation protein-1 (AP-1) site in the MMP-9 promoter. These findings indicate that BITC is an effective agent for inhibiting cell proliferation, the G1 to S phase cell cycle progress, and MMP-9 expression through the transcription factors NF-kappaB and AP-1 in TNF-alpha-induced VSMC.Journal of Cellular Physiology 07/2005; 203(3):493-500. · 3.87 Impact Factor -
Article: Large-scale and effective screening of Korean medicinal plants for inhibitory activity on matrix metalloproteinase-9.
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ABSTRACT: Matrix metalloproteinase-9 (MMP-9) degrades type IV collagen constituting the major structural component of the basement membrane and extra cellular membrane. The enzymatic activity is found to be elevated in tumor tissues. With the aim of finding novel MMP-9 inhibitors from natural products, 87 extracts of oriental medicinal herbs, which are used as prescriptions for cancer treatment in traditional Korean medicine, were screened for their inhibitory activities towards MMP-9. It was found that most of the hexane and chloroform fractions as well as water extracts showed a weak inhibitory effect on MMP-9 activity at a concentration of 100mug/ml. However, a strong inhibition was found in the butanol fractions of Cinnamomum cassia PRESL, Magnolia obovata THUEB., Magnolia officinalis REHD. et WILS., Magnolia officinalis REHD. et WILS. var. biloba REHD. et WILS., and Euonymus alatus (THUNB.) SIEB. with inhibitory activity (>90%) at a concentration of 100 microg/ml.Journal of Ethnopharmacology 02/2005; 97(1):101-6. · 3.01 Impact Factor -
Article: Inhibitory effect of GBH on platelet aggregation through inhibition of intracellular Ca2+ mobilization in activated human platelets.
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ABSTRACT: Geiji-Bokryung-Hwan (GBH) was studied on antiplatelet activity in human platelet suspensions. GBH consists of the 5 herbs Cinnamomi Ramulus, Poria Cocos, Mountan Cortex Radicis, Paeoniae Radix, and Persicae Semen, which have been used in herbal medicine for thousands of years for atherosclerosis. The mechanism involved in the antiplatelet activity of GBH in human platelet suspensions was investigated. GBH inhibited platelet aggregation and Ca2+ mobilization in a concentration-dependent manner without increasing intracellular cyclic AMP and cyclic GMP. GBH had no inhibitory effect on thromboxane B2 (TXB2) production in cell-free systems. Collagen-related peptide (CRP)-induced Ca2+ mobilization is regulated by phospholipase C-2 (PLC-gamma2) activation. We evaluated the effect of GBH on tyrosine phosphorylation of PLC-gamma2 and the production of inositol-1,4,5-trisphosphate (IP3). GBH at concentrations that inhibited platelet aggregation and Ca2+ mobilization had no effects on tyrosine phosphorylation of PLC-gamma2 or on the formation of IP3 induced by CRP. Similar results were obtained with thrombin-induced platelet activation. GBH inhibited platelet aggregation and Ca2+ mobilization induced by thrombin without affecting the production of IP3. We then evaluated the effect of GBH on the binding of IP3 to its receptor. GBH at high concentrations partially blocked the binding of IP3 to its receptor. Therefore, the results suggested that GBH suppresses Ca2+ mobilization at a step distal to IP3 formation. GBH may provide a good tool for investigating Ca2+ mobilization.Life Sciences 12/2004; 75(25):3063-76. · 2.53 Impact Factor -
Article: Induction of a TC1-mediated experimental autoimmune thyroiditis by deglycosylated porcine thyroglobulin.
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ABSTRACT: Contribution of cytotoxic T lymphocytes (CTL) to experimental autoimmune thyroiditis (EAT) was well defined (Speidel et al., Eur. J. Immunol. 1997, 27, 2391-2399, Ref. 7). The native porcine thyroglobulin (pTg) showed high sensitivity to endo-o-N-acetylglucosaminidase F (Endo F) and its molecular weights, corresponding to about 330 kDa as a monomer and 660 kDa as a dimer, were reduced to smaller molecular weight forms by Endo F and trifluoromethanesulfonic acid (TMSF). Deglycosylated porcine Tg (dgpTg) and native pTg were injected i.v. into CBA/J mice, without the aid of adjuvants. Both lymphocytic infiltrations of the thyroid glands and levels of Tg-specific CTL were similar to those found in conventional EAT induced by Tg and adjuvants. In contrast, proliferative responses in native pTg and dgpTg-injected mice could not be detected, and titers of antibodies to pTg and dgpTg were 20 times and 30 times lower than that of pTg and adjuvants, respectively. The EAT-inducer CTL belonged to the CD8+ cell subset and exerted their thyroiditogenic potential through release of IFN-gamma. It was concluded that dgpTg-induced EAT is mediated by type 1 cytotoxic T cells (Tcl). Also, results that EAT induction of the glycosylated pTg (gpTg) was much lower than that of dgpTg, suggested that the abberant and incomplete glycosylation of the thyroglobulin is responsible for the induction of autoimmune thyroiditis.Immunopharmacology and Immunotoxicology 09/2004; 26(3):355-72. · 1.83 Impact Factor -
Article: Protective effect of Moutan Cortex extract on acetaminophen-induced hepatotoxicity in mice.
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ABSTRACT: Previously, we demonstrated that Moutan Cortex prevents acetaminophen (AAP)-induced cytotoxicity in vitro. The present study examined the protective effect of Moutan Cortex on AAP induced hepatotoxicity and the possible mechanisms underlying this effect in mice. When Montan Cortex was administered to ICR mice, followed by hepatotoxic dose of AAP (400 mg/kg, i.p.), Moutan Cortex pre-exposure prevented liver injury as indicated by the decrease of serum alanine aminotransferase level. Moutan Cortex also protected AAP-induced hepatic glutathione depletion. Cytochrome P450 2E1-dependent aniline and p-nitrophenol hydroxylases activities in microsomal incubations were significantly inhibited by Moutan Cortex. Abrogation of toxicity was also mirrored in DNA fragmentation. These observations demonstrate that Moutan Cortex pre-exposure may attenuate AAP-induced GSH depletion, cytochrome P450 2E1 activity, and hepatic DNA damage in vivo.Journal of Ethnopharmacology 03/2004; 90(2-3):415-9. · 3.01 Impact Factor -
Article: Effect of herbal medicines on cytokine-induced cytotoxicity and major histocompatibility complex (MHC) class II antigen expression in rat thyroid cells.
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ABSTRACT: The effects of herbal medicines that constitute Gam-du-tang and Gung-gui-tang on cytokine-induced cytotoxicity and thyroid major histocompatibility complex (MHC) class II antigen expression in FRTL rat thyrocytes were investigated. No effect on cell growth was found with interferon (IFN)-gamma. However, tumor necrosis factor (TNF)-alpha was cytotoxic, and this was increased by preincubation with IFN-gamma. Ethanol extract of Glycyrrhizae Radix, black beans, Angelicae Radix, and Cnidii Rhizoma (0.3-15 mg/ml) in both regimens significantly inhibited IFN-gamma and TNF-alpha-mediated cytotoxicity of rat thyroid cells (p<0.05, p<0.01). In addition, IFN-gamma (1-100 U/ml) treatment induced class II antigen expression in up to 60% of FRTL cells, as detected by a murine monoclonal antibody to rat MHC class II antigen (FITC-OX6). Aberrant thyroid cell MHC class II antigen expression induced by IFN-gamma is suppressed by the extract of herbal medicines. These results indicate that herbal medicines inhibit cytokine-induced thyroid cell destruction, therefore, may have therapeutic potential in the treatment of autoimmune thyroid disease.Biological & Pharmaceutical Bulletin 03/2004; 27(3):371-4. · 1.66 Impact Factor -
Article: Inhibition of cytochrome P450 isozymes and ornithine decarboxylase activities by polysaccharides from soybeans fermented with Phellinus igniarius or Agrocybe cylindracea.
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ABSTRACT: Polysaccharides (5, 10, 25, 50 and 100 microg ml(-1)) from soybeans and soybeans fermented with Phellinus igniarius or Agrocybe cylindracea inhibited cytochrome P450 1A1, cytochrome P450 1A2 and cytochrome P450 2B1 activities in rat liver microsomes. The polysaccharides (5, 10 and 25 microg ml(-1)) also suppressed 12-O-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase activity. The most potent inhibitors of cytochrome P450 isozymes and ornithine decarboxylase activities were the polysaccharides from soybeans fermented with Agrocybe cylindracea.Biotechnology Letters 02/2004; 26(2):159-63. · 1.68 Impact Factor -
Article: Age-related changes in matrix metalloproteinase-9 regulation in cultured mouse aortic smooth muscle cells.
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ABSTRACT: We previously reported that aortic smooth muscle cells (SMC) from aged mice have an age-related decline in proliferative capacity compared with those derived from young mice. Here we investigated matrix metalloproteinase-9 (MMP-9) regulation in both young and aged SMC. Zymography, immunoblot, and northern blot analysis showed that MMP-9 expression is significantly reduced in response to tumor necrosis factor-alpha stimulation with increasing in vitro age. Mutational analysis, gel shift assays and supershift assays demonstrated that the lower MMP-9 expression in aged SMC is associated with lower activities of NF-kappaB and AP-1. Since mitogen-activated protein kinase ERK1/2 induce MMP-9 expression, we examined whether U0126, an ERK1/2 inhibitor, influenced MMP-9 expression in aged SMC. Treatment with U0126 successfully inhibited MMP-9 expression in both young and aged SMC. Finally, to analyze the causal relationship between replicative senescence and MMP-9 expression, we stably overexpressed the MMP-9 gene in aged SMC and we showed no alteration of the proliferative capacity of the transduced cells. Taken together, these results suggest that down-regulation of MMP-9 expression in SMC may play a role in vascular remodeling during in vitro aging.Experimental Gerontology 02/2004; 39(1):123-31. · 3.74 Impact Factor -
Article: Protective effect of Astragali radix extract on interleukin 1beta-induced in fl ammation in human amnion.
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ABSTRACT: The aim of this study was to investigate the effect of Astragali radix extract on interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha production and prostaglandin E(2) (PGE(2)) and leukotriene C(4) (LTC(4)) release from IL-1beta-stimulated human amnion. Primary monolayer cultures of amnion cells were established from women undergoing elective caesarean section before the onset of labour. Production of both IL-6 and TNF-alpha was stimulated in a concentration-dependent manner by proinflammatory cytokine IL-1beta (0.01-10 ng/mL). Astragalus extract inhibited IL-6 production by approximately 75% from cells under IL-1beta-stimulated conditions. Treatment of amnion cells with IL-1beta (0.01-10 ng/mL) resulted in a significant increase in PGE(2) release. Incubation of the cells with the extract for 24 h significantly inhibited IL-1beta-induced PGE(2) production. A concentration-dependent increase in LTC(4) production by amnion cells occurred in response to IL-1beta. Astragalus extract blocked the effect of IL-1beta in LTC(4) production in human amnion. These results indicate that Astragali radix has a broad antiinflammatory effect in human amnion and may be considered a promising agent to protect preterm labour.Phytotherapy Research 12/2003; 17(9):1016-20. · 2.09 Impact Factor -
Article: Protective effect of Astragali radix extract on interleukin 1β‐induced inflammation in human amnion
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ABSTRACT: The aim of this study was to investigate the effect of Astragali radix extract on interleukin (IL)-6 and tumour necrosis factor (TNF)- production and prostaglandin E2 (PGE2) and leukotriene C4 (LTC4) release from IL-1β-stimulated human amnion. Primary monolayer cultures of amnion cells were established from women undergoing elective caesarean section before the onset of labour. Production of both IL-6 and TNF- was stimulated in a concentration-dependent manner by proinflammatory cytokine IL-1β (0.01–10 ng/mL). Astragalus extract inhibited IL-6 production by approximately 75% from cells under IL-1β-stimulated conditions. Treatment of amnion cells with IL-1β (0.01–10 ng/mL) resulted in a significant increase in PGE2 release. Incubation of the cells with the extract for 24 h significantly inhibited IL-1β-induced PGE2 production. A concentration-dependent increase in LTC4 production by amnion cells occurred in response to IL-1β. Astragalus extract blocked the effect of IL-1β in LTC4 production in human amnion. These results indicate that Astragali radix has a broad antiinflammatory effect in human amnion and may be considered a promising agent to protect preterm labour. Copyright © 2003 John Wiley & Sons, Ltd.Phytotherapy Research 10/2003; 17(9):1016 - 1020. · 2.09 Impact Factor -
Article: Inhibition of polyamine biosynthesis in Acanthamoeba castellanii and 12-O-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase activity by chitosanoligosaccharide.
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ABSTRACT: Growth of Acanthamoeba castellanii was inhibited by chitosanoligosaccharide (up to 20 mg ml-1) from the shells of crabs but was reversed by the polyamines, putrescine or spermidine, at 0.8 mM. Chitosanoligosaccharide strongly inhibited the induction of ornithine decarboxylase by 12-O-tetradecanoylphorbol-13-acetate, a key enzyme of polyamine biosynthesis, which is enhanced in tumour promotion.Biotechnology Letters 06/2003; 25(9):701-4. · 1.68 Impact Factor
Top co-authors
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Institutions
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2001–2009
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Dongguk University
Seoul, Seoul, South Korea
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2000
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Yeungnam University
Asan, South Chungcheong, South Korea
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