Keith Anderson

Publications (3)67.48 Total impact

• Source

  Available from: Stefano Campanaro

  Dataset: Functional profiling of the Saccharomyces cerevisiae genome

  Guri Giaever, Angela M Chu, Li Ni, Carla Connelly, Linda Riles, Steeve Véronneau, Sally Dow, Ankuta Lucau-Danila, Keith Anderson, Bruno André, [......], Yonghong Yang, Grace Yen, Elaine Youngman, Kexin Yu, Howard Bussey, Jef D Boeke, Michael Snyder, Peter Philippsen, Ronald W Davis, Mark Johnston
• Source

  Available from: Stefano Campanaro

  Article: Functional profiling of the Saccharomyces cerevisiae genome.

  Guri Giaever, Angela M Chu, Li Ni, Carla Connelly, Linda Riles, Steeve Véronneau, Sally Dow, Ankuta Lucau-Danila, Keith Anderson, Bruno André, [......], Yonghong Yang, Grace Yen, Elaine Youngman, Kexin Yu, Howard Bussey, Jef D Boeke, Michael Snyder, Peter Philippsen, Ronald W Davis, Mark Johnston
  [show abstract] [hide abstract]
  ABSTRACT: Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed 'molecular bar codes' uniquely identify each strain, enabling their growth to be analysed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays. We show that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment. Less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal growth in four of the tested conditions. Our results validate the yeast gene-deletion collection as a valuable resource for functional genomics.
  Nature 08/2002; 418(6896):387-91. · 36.28 Impact Factor
• Article: Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis

  Elizabeth A. Winzeler, Daniel D. Shoemaker, Anna Astromoff, Hong Liang, Keith Anderson, Bruno Andre, Rhonda Bangham, Rocio Benito, Jef D. Boeke, Howard Bussey, [......], Marleen Voet, Guido Volckaert, Teresa R. Ward, Robert Wysocki, Grace S. Yen, Kexin Yu, Katja Zimmermann, Peter Philippsen, Mark Johnston, Ronald W. Davis
  [show abstract] [hide abstract]
  ABSTRACT: The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.
  Science 08/1999; 285(5429):901-906. · 31.20 Impact Factor