Jingyi Chen

Publications of Jingyi Chen

• Mechanism-based tumor-targeting drug delivery system. Validation of efficient vitamin receptor-mediated endocytosis and drug release.

  Authors: Shuyi Chen, Xianrui Zhao, Jingyi Chen, Jin Chen, Larisa Kuznetsova, Stanislaus S Wong, Iwao Ojima

  Bioconjugate chemistry. 05/2010; 21(5):979-87.

  An efficient mechanism-based tumor-targeting drug delivery system, based on tumor-specific vitamin-receptor mediated endocytosis, has been developed. The tumor-targeting drug delivery system is aAn efficient mechanism-based tumor-targeting drug delivery system, based on tumor-specific vitamin-receptor mediated endocytosis, has been developed. The tumor-targeting drug delivery system is a conjugate of a tumor-targeting molecule (biotin: vitamin H or vitamin B-7), a mechanism-based self-immolative linker and a second-generation taxoid (SB-T-1214) as the cytotoxic agent. This conjugate (1) is designed to be (i) specific to the vitamin receptors overexpressed on tumor cell surface and (ii) internalized efficiently through receptor-mediated endocytosis, followed by smooth drug release via glutathione-triggered self-immolation of the linker. In order to monitor and validate the sequence of events hypothesized, i.e., receptor-mediated endocytosis of the conjugate, drug release, and drug-binding to the target protein (microtubules), three fluorescent/fluorogenic molecular probes (2, 3, and 4) were designed and synthesized. The actual occurrence of these processes was unambiguously confirmed by means of confocal fluorescence microscopy (CFM) and flow cytometry using L1210FR leukemia cells, overexpressing biotin receptors. The molecular probe 4, bearing the taxoid linked to fluorescein, was also used to examine the cell specificity (i.e., efficacy of receptor-based cell targeting) for three cell lines, L1210FR (biotin receptors overexpressed), L1210 (biotin receptors not overexpressed), and WI38 (normal human lung fibroblast, biotin receptor negative). As anticipated, the molecular probe 4 exhibited high specificity only to L1210FR. To confirm the direct correlation between the cell-specific drug delivery and anticancer activity of the probe 4, its cytotoxicity against these three cell lines was also examined. The results clearly showed a good correlation between the two methods. In the same manner, excellent cell-specific cytotoxicity of the conjugate 1 (without fluorescein attachment to the taxoid) against the same three cell lines was confirmed. This mechanism-based tumor-targeting drug delivery system will find a range of applications.

• Evaluating cytotoxicity and cellular uptake from the presence of variously processed TiO2 nanostructured morphologies.

  Authors: Jingyi Chen, Hongjun Zhou, Alexander C Santulli, Stanislaus S Wong

  Chemical research in toxicology. 05/2010; 23(5):871-9.

  We evaluated the cytotoxicity of various morphological classes of TiO(2) nanostructures (including 0-D nanoparticles, 1-D nanorods, and 3-D assemblies) toward living cells. These TiO(2)We evaluated the cytotoxicity of various morphological classes of TiO(2) nanostructures (including 0-D nanoparticles, 1-D nanorods, and 3-D assemblies) toward living cells. These TiO(2) nanostructures were modified with fluorescent dye molecules, mediated via a dopamine linkage, in order to facilitate a confocal study of their internalization. Specifically, we noted that both TiO(2) 1-D nanorods and 0-D nanoparticles could internalize into cells after 24 h of incubation time. However, only incubation with TiO(2) 1-D nanorods and 3-D micrometer-scale sea urchin-like assemblies at concentrations of up to 125 microg/mL yielded data suggestive of cell viabilities of close to 100%. Moreover, upon irradiation with UV light for periods of a few minutes at energy densities of up to 1 J/cm(2), we observed up to 60% mortality rates, indicative of the cytotoxic potential of photoirradiated TiO(2) nanostructures due to the generation of reactive oxygen species.

• Water-Dispersible, Multifunctional, Magnetic, Luminescent Silica-Encapsulated Composite Nanotubes.

  Authors: Hongjun Zhou, Jingyi Chen, Eli Sutter, Mikhail Feygenson, M C Aronson, Stanislaus S Wong

  Small (Weinheim an der Bergstrasse, Germany). 12/2009;

  A multifunctional one-dimensional nanostructure incorporating both CdSe quantum dots (QDs) and Fe(3)O(4) nanoparticles (NPs) within a SiO(2)-nanotube matrix is successfully synthesized based on theA multifunctional one-dimensional nanostructure incorporating both CdSe quantum dots (QDs) and Fe(3)O(4) nanoparticles (NPs) within a SiO(2)-nanotube matrix is successfully synthesized based on the self-assembly of preformed functional NPs, allowing for control over the size and amount of NPs contained within the composite nanostructures. This specific nanostructure is distinctive because both the favorable photoluminescent and magnetic properties of QD and NP building blocks are incorporated and retained within the final silica-based composite, thus rendering it susceptible to both magnetic guidance and optical tracking. Moreover, the resulting hydrophilic nanocomposites are found to easily enter into the interiors of HeLa cells without damage, thereby highlighting their capability not only as fluorescent probes but also as possible drug-delivery vehicles of interest in nanobiotechnology.

• Carbon nanotube-nanocrystal heterostructures.

  Authors: Xiaohui Peng, Jingyi Chen, James A Misewich, Stanislaus S Wong

  Chemical Society reviews. 05/2009; 38(4):1076-98.

  The importance of generating carbon nanotube-nanoparticle heterostructures is that these composites ought to take advantage of and combine the unique physical and chemical properties of both carbonThe importance of generating carbon nanotube-nanoparticle heterostructures is that these composites ought to take advantage of and combine the unique physical and chemical properties of both carbon nanotubes and nanoparticles in one discrete structure. These materials have potential applicability in a range of diverse fields spanning heterogeneous catalysis to optoelectronic device development, of importance to chemists, physicists, materials scientists, and engineers. In this critical review, we present a host of diverse, complementary strategies for the reliable synthesis of carbon nanotube-nanoparticle heterostructures using both covalent as well as non-covalent protocols, incorporating not only single-walled and multi-walled carbon nanotubes but also diverse classes of metallic and semiconducting nanoparticles (221 references).

• Functionalized single-walled carbon nanotubes as rationally designed vehicles for tumor-targeted drug delivery.

  Authors: Jingyi Chen, Shuyi Chen, Xianrui Zhao, Larisa V Kuznetsova, Stanislaus S Wong, Iwao Ojima

  Journal of the American Chemical Society. 01/2009; 130(49):16778-85.

  A novel single-walled carbon nanotube (SWNT)-based tumor-targeted drug delivery system (DDS) has been developed, which consists of a functionalized SWNT linked to tumor-targeting modules as well asA novel single-walled carbon nanotube (SWNT)-based tumor-targeted drug delivery system (DDS) has been developed, which consists of a functionalized SWNT linked to tumor-targeting modules as well as prodrug modules. There are three key features of this nanoscale DDS: (a) use of functionalized SWNTs as a biocompatible platform for the delivery of therapeutic drugs or diagnostics, (b) conjugation of prodrug modules of an anticancer agent (taxoid with a cleavable linker) that is activated to its cytotoxic form inside the tumor cells upon internalization and in situ drug release, and (c) attachment of tumor-recognition modules (biotin and a spacer) to the nanotube surface. To prove the efficacy of this DDS, three fluorescent and fluorogenic molecular probes were designed, synthesized, characterized, and subjected to the analysis of the receptor-mediated endocytosis and drug release inside the cancer cells (L1210FR leukemia cell line) by means of confocal fluorescence microscopy. The specificity and cytotoxicity of the conjugate have also been assessed and compared with L1210 and human noncancerous cell lines. Then, it has unambiguously been proven that this tumor-targeting DDS works exactly as designed and shows high potency toward specific cancer cell lines, thereby forming a solid foundation for further development.

• Functionalized Single-Walled Carbon Nanotubes as Rationally Designed Vehicles for Tumor-Targeted Drug Delivery.

  Authors: Jingyi Chen, Shuyi Chen, Xianrui Zhao, Larisa Kuznetsova, Stanislaus Wong, Iwao Ojima

  Journal of the American Chemical Society. 12/2008;

  A novel single-walled carbon nanotube (SWNT)-based tumor-targeted drug delivery system (DDS) has been developed, which consists of a functionalized SWNT linked to tumor-targeting modules as well asA novel single-walled carbon nanotube (SWNT)-based tumor-targeted drug delivery system (DDS) has been developed, which consists of a functionalized SWNT linked to tumor-targeting modules as well as prodrug modules. There are three key features of this nanoscale DDS: (a) use of functionalized SWNTs as a biocompatible platform for the delivery of therapeutic drugs or diagnostics, (b) conjugation of prodrug modules of an anticancer agent (taxoid with a cleavable linker) that is activated to its cytotoxic form inside the tumor cells upon internalization and in situ drug release, and (c) attachment of tumor-recognition modules (biotin and a spacer) to the nanotube surface. To prove the efficacy of this DDS, three fluorescent and fluorogenic molecular probes were designed, synthesized, characterized, and subjected to the analysis of the receptor-mediated endocytosis and drug release inside the cancer cells (L1210FR leukemia cell line) by means of confocal fluorescence microscopy. The specificity and cytotoxicity of the conjugate have also been assessed and compared with L1210 and human noncancerous cell lines. Then, it has unambiguously been proven that this tumor-targeting DDS works exactly as designed and shows high potency toward specific cancer cell lines, thereby forming a solid foundation for further development.