Jinyang Zhang

Tianjin Polytechnic University, T’ien-ching-shih, Tianjin Shi, China

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Publications (6)9.65 Total impact

  • Minling Gao · Wenhua Song · Jinyang Zhang · Jing Guo ·
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    ABSTRACT: Earthworms are an ideal biological model in toxicity assays and environment monitoring studies, especially for the toxicity of pesticides on soil ecosystem. However, There are very little data on the toxicity of triazoles on earthworms despite the fact that such data are critical in assessing their fate and potential toxic effects in soil organisms. To address this issue, earthworms were exposed to triazoles (triadimefon, triadimenol, difenoconazole and propiconazole) to study biochemical and histopathological examination. The results showed protein content significantly increased in treatment of difenoconazole compared to control. There were no significant differences between controls and triadimefon treated groups, while the glutathione peroxidase (GSH-Px) activity is significantly lower than control. Other triazoles also had an inhibitory effect on GSH-Px activity at higher concentration. The histopathological examination showed the epidermis and the epidermis cell of earthworm was ruined at lower triazoles concentration. The arrangement of smooth muscle layer disordered, and some cell disintegrated with concentration increasing of pesticides. Cell pyknosis, cytoplasm deep stained, nucleus concentrations were observed in the treated group with propiconazole.
    02/2013; 35(3):427-433. DOI:10.1016/j.etap.2013.02.003
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    ABSTRACT: With large-scale production and wide application of nano-titanium oxide (TiO(2)), its health hazard has attracted extensive attention worldwide. In this study, mouse macrophages (Ana-1 and MH-S cells) were used to evaluate the cytotoxicity of different sized TiO(2) nanoparticles. The results showed that TiO(2) nanoparticles caused low toxicity, especially in MH-S cells. There was a difference in the cytotoxicity induced by different sized TiO(2) particles. The 25 nm anatase particles induced the strongest cytotoxicity and oxidative stress, followed by 5 and 100 nm anatase particles; in contrast, 100 nm rutile particles induced the lowest toxicity. Although TiO(2) nanoparticles induced high levels of intracellular reactive oxygen species (ROS), the determination of ROS demonstrated that the inherent oxidative capacity of TiO(2) nanoparticles was lower in the absence of photoactivation. Therefore, the generation of intracellular ROS could not completely depend on inherent oxidative capacity of TiO(2) nanoparticles. Toxicity of TiO(2) nanoparticles could mainly depend on the structural characteristics.
    Toxicology and Industrial Health 04/2012; 29(6). DOI:10.1177/0748233712442708 · 1.86 Impact Factor
  • Jinyang Zhang · Wenhua Song · Jing Guo · Jinhua Zhang · Zengtian Sun · Feng Ding · Minling Gao ·
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    ABSTRACT: To study the toxicity mechanism of ZnO nanoparticles on mouse macrophages, the toxic effect of different ZnO nanoparticles on mouse alveolar macrophages (MH-S) was investigated in this study. The results showed that the 24h IC(50) of four ZnO particles were 48.53, 47.37, 45.43 and 26.74 μg/ml for bulk ZnO, 100 nm, 30 nm and 10-30 nm ZnO particles, respectively. At the concentration of 10 μg/ml and below, dissolved zinc ions induced metallothionein synthesis, enhanced cellular resistance to oxidative stress. ZnO particles mainly induced cell apoptosis. When the concentration of ZnO particles was 20 μg/ml and above, excessive zinc destroyed mitochondrial function and cell membrane, caused cell necrosis. Dissolved zinc ions first cause toxicity in MH-S cells. However, the toxic effect of dissolved zinc ions may exist a threshold on mouse macrophages, inducing about 50% cell death. The toxic difference of different ZnO particles mainly depended on the effect of nondissolved ZnO particles.
    Journal of hazardous materials 04/2012; 219-220:148-55. DOI:10.1016/j.jhazmat.2012.03.069 · 4.53 Impact Factor
  • Jing Guo · Wenhua Song · Feng Ding · Jinyang Zhang · Zengtian Sun ·
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    ABSTRACT: The acute cytotoxicities of six naphthoquinone compounds, including Atovaquone, Buparvaquone, Menadione, 2-acetoxy-1,4-naphthoquinone and 2-ethoxy-1,4-naphthoquinone, to HL-7702 cells were determined. The results showed that the toxicities of these naphthoquinones were characterized by a steep response pattern except for 2-hydroxy-1,4-naphthoquinone. Meanwhile, the cellular injuries were unrecoverable. Several molecular descriptors, such as the octanol-water partition coefficients (LogP), diameter (Dia) and topological index (TIndx), played an important role in the toxicity of naphthoquinones to HL-7702 cell. Our results provide a foundation for further investigation using 3D-QSAR and HQSAR to evaluate the aquatic ecological risk and the possible mechanisms of toxicity of naphthoquinones.
    01/2012; 33(3):408-13. DOI:10.1016/j.etap.2012.01.005
  • Wenhua Song · Jinyang Zhang · Jing Guo · Jinhua Zhang · Feng Ding · Liying Li · Zengtian Sun ·
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    ABSTRACT: With large-scale production and wide application of nanoscale ZnO, its health hazard has attracted extensive worldwide attention. In this study, cytotoxicity of different sized and shaped ZnO nanoparticles in mouse macrophage Ana-1 was investigated. And contribution of dissolved Zn(2+) and ROS in toxicity of ZnO particles was analyzed. The results indicated that ZnO particles manifested dose-dependent toxic effect on Ana-1 cells without size-dependence, and the particles shape may impact cytotoxicity of ZnO particles. When the concentration of dissolved Zn(2+) tended to equilibrium in the complete cell medium, the zinc ion concentration was approximately 10 μg/ml, inducing about 50% cell death, which was close to the cytotoxicity of ZnCl(2) (IC(50)=13.33 μg Zn/ml). The Zn(2+) concentration had significant correlations with cell viability and LDH level induced by the supernatant of ZnO particle suspensions (incubation at 37°C for 24h). Thus, the dissolved Zn(2+) played the main role in toxic effect of ZnO particles. Moreover, ROS generation assays demonstrated that ZnO particles produced intrinsically a small quantity of ROS, intracellular ROS was mainly produced after ZnO particles or the dissolved Zn(2+) entered into the cells. Although intracellular ROS had significant correlations with cell viability and LDH induced by ZnO particles, intracellular ROS may not be a major factor in cytotoxicity of ZnO nanoparticles, but the cytotoxic response.
    Toxicology Letters 10/2010; 199(3):389-97. DOI:10.1016/j.toxlet.2010.10.003 · 3.26 Impact Factor
  • Wenhua Song · Jinhua Zhang · Jinyang Zhang ·
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    ABSTRACT: Acute toxicity of different sized (10, 30 and lOOnm) ZnO particles was investigated by intratracheal injection at the dose of 0.05 and 0.5g/kg body weight. The results of biochemical parameters assay, pathological examination and zinc accumulation showed main target organs for ZnO particles are demonstrated as lung, spleen, pancreas, bone and liver after exposure by intratracheal injection. But the toxic effects between different sized and dose ZnO particles are a little different. The toxicity of ZnO nanoparticles exhibited a dose-effect relationship. At low dose, three sized ZnO particles induce slight damage, and lOnm ZnO particles lead to relatively serious lung and liver damage. At high dose, 30nm ZnO particles result in worse lung, liver and pancreas injury. Furthermore, ZnO nanoparticles led to oxide stress, which may be an important reason for injury induced by ZnO particles.
    01/2010; DOI:10.1109/ICBBE.2010.5515207

Publication Stats

179 Citations
9.65 Total Impact Points


  • 2013
    • Tianjin Polytechnic University
      T’ien-ching-shih, Tianjin Shi, China
  • 2010-2012
    • Shanghai Jiao Tong University
      • School of Environmental Science and Engineering
      Shanghai, Shanghai Shi, China