Jin Zhao

Publications (2)3.66 Total impact

• Article: Identification of a novel box C/D snoRNA from mouse nucleolar cDNA library.

  Hui Zhou, Jin Zhao, Chuan-He Yu, Qing-Jun Luo, Yue-Qin Chen, Yu Xiao, Liang-Hu Qu
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  ABSTRACT: By construction and screen of mouse nucleolar cDNA library, a novel mammalian small nucleolar RNAs (snoRNA) was identified. The novel snoRNA, 70 nt in length, displays structural features typical of C/D box snoRNA family. The snoRNA possesses an 11-nt-long rRNA antisense element and is predicted to guide the 2'-O-methylation of mouse 28S rRNA at G4043, a site unknown so far to be modified in vertebrates. The comparison of functional element of snoRNA guides among eukaryotes reveals that the novel snoRNA is a mammalian counterpart of yeast snR38 despite highly divergent sequence between them. Mouse and human snR38 and other cognates in distant vertebrates were positively detected with slight length variability. As expected, the rRNA ribose-methylation site predicted by mouse snR38 was precisely mapped by specific-primer extension assay. Furthermore, our analyses show that mouse and human snR38 gene have multiple variants and are nested in the introns of different host genes with unknown function. Thus, snR38 is a phylogenetically conserved methylation guide but exhibits different genomic organization in eukaryotes.
  Gene 03/2004; 327(1):99-105. · 2.34 Impact Factor
• Article: Z3, a novel antisense snoRNA from Saccharomyces cerevisiae

  Yongjun Lu, Ling Zhong, Hui Zhou, Jin Zhao, Lianghu Qu
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  ABSTRACT: Small nuclrular RNA (snoRNA) is one of the most important elements participating in eukaryotic ribosomal biogenesis. The present report describes the results of the identification of a novel snoRNA, Z3, from yeast S.cerevisiae. Z3 snoRNA is 106 nts in length. It contains box C, D elements and a 13 nt complementarity to 25S rRNA. Thia antisense segment, together with the downstream box D, guides a 2′-O-ribose methylation of cytidine acid at position 2956th in yeaat 25 S rRNA. Z3 snoRNA, encoded by an independent transcribed gene on the chromosomeXI of yeast, poseses the same functional elements responsible for the rRNA methylation site as that of the intron-encoded U35 snoRNA of vertebrate.
  Chinese Science Bulletin 03/1998; 43(7):586-590. · 1.32 Impact Factor