Publications (2)1.96 Total impact

  • Jianwu Li · He Zhang · Jianbin Hu · Junqing Liu · Keke Liu
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    ABSTRACT: Heat shock proteins (Hsps) are a family of highly conserved proteins present in all organisms. They mediate a range of cytoprotective functions as molecular chaperones and are recently reported to regulate the immune response. Using suppression subtractive hybridization, we isolated and characterized a cucumber cDNA, designated CsHsp45.9, which encodes a putative heat shock protein of 45.9 kDa protein, containing three conserved DnaJ domains belonging to the Type I Hsp40 family. Real-time quantitative RT-PCR analysis revealed that CsHsp45.9 was significantly induced in cucumber leaves inoculated with downy mildew (Pseudoperonospora cubensis) in this incompatible interaction. Gene expression was also strongly up-regulated by various abiotic stresses. CsHsp45.9 was mainly expressed in flowers with a flower-specific, stamen- and pistil-predominant expression pattern. This suggests that CsHsp45.9 harbors broad-spectrum responses to both biotic and abiotic stresses and may play a role in downy mildew resistance in cucumber.
    Biochemical Genetics 02/2012; 50(7-8):565-78. DOI:10.1007/s10528-012-9501-9 · 0.87 Impact Factor
  • Journal of Genetics 08/2010; 89(2):e28-32. DOI:10.1007/s12041-011-0005-x · 1.09 Impact Factor