Ildikó- Márton

Publications (6)17.4 Total impact

• Article: [The prevalance of herpesviruses in human apical periodontitis samples].

  Katinka Hernádi, Anita Szalmás, Richard Mogyorósi, Levente Czompa, György Veress, Eszter Csoma, Ildikó Márton, József Kónya
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  ABSTRACT: Apical periodontitis is primarily initiated by the endodonto-patogen bacteria spreading from the inflamed or necrotic pulp tissues to the periapical area. Nevertheless, findings within the past years have established a pathogenic role of human herpesviruses such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) in periapical inflammations. The authors analysed the prevalence, activity and disease association of EBV, HCMV and human herpesvirus 6 (HHV-6) in 40 apical periodontitis samples and 40 healthy pulp controls. Based on the viral DNA results, EBV (29/40) was the most frequent herpesvirus in apical periodontitis, followed by HHV-6 (8/40) and HCMV (4/40). According to the mRNA results approximately two-third of the EBV DNA-positive lesions had active EBV infections. However, the HHV-6 and the HCMV infections seemed to be of latent state. Our findings suggest that EBV and HHV-GB infections primarily occurred in large sized and symptomatic periapical lesions. The co-occurrence of large lesion size and active EBV infection was strongly associated (OR = 8.80) with the symptomatic manifestation of apical periodontitis.
  Fogorvosi szemle 12/2012; 105(4):135-40.
• Article: Association of human herpesvirus 6 subtypes with symptomatic apical periodontitis.

  Katinka Hernádi, Eszter Csoma, Balázs Adám, Anita Szalmás, Eszter Gyöngyösi, György Veress, Ildikó-Márton, József Kónya
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  ABSTRACT: The occurrence of human herpesvirus (HHV) 6 subtypes A and B in apical periodontitis was determined. The relationship of HHV-6 subtypes to other disease associated herpesviruses, i.e., Epstein-Barr virus (EBV) and human cytomegalovirus, was also investigated. Forty apical periodontitis samples (17 symptomatic and 23 asymptomatic) and 40 healthy pulp control samples were collected. Nested polymerase chain reaction was used to detect HHV-6 DNA. HHV-6 DNA was observed in significantly higher frequencies in apical periodontitis samples than in control samples (20% vs. 2.5%; P = .03). Further classification of apical lesions revealed that subtype B of HHV-6 was significantly associated with large-sized and symptomatic lesions (P < .01). Thirty-one apical lesions (77%) harbored ≥1 of the tested herpesviruses: EBV was the most frequent herpesvirus (72.5%) in apical periodontitis, followed by HHV-6 (20%). Our findings suggest that EBV and HHV-6B infections can be associated with symptomatic apical periodontitis.
  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 05/2011; 112(3):401-6. · 1.50 Impact Factor
• Article: Prevalence and activity of Epstein-Barr virus and human cytomegalovirus in symptomatic and asymptomatic apical periodontitis lesions.

  Katinka Hernádi, Anita Szalmás, Richárd Mogyorósi, Levente Czompa, György Veress, Eszter Csoma, Ildikó Márton, József Kónya
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  ABSTRACT: Apical periodontitis is a polymicrobial inflammation with a dominant flora of opportunistic Gram-negative bacteria; however, a pathogenic role of human herpesviruses such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) has been implicated recently. The aims of this study were to determine the prevalence, activity, and disease association of EBV and HCMV in apical periodontitis in an Eastern Hungarian population. Forty samples with apical periodontitis (17 symptomatic and 23 asymptomatic) and 40 healthy pulp controls were collected. EBV and HCMV prevalences were measured by polymerase chain reaction (PCR) detection of the viral DNA and viral activity was tested by reverse-transcription PCR amplification of viral messenger RNA. EBV DNA and EBNA-2 messenger RNA were found in apical periodontitis lesions at significantly (p < 0.0001) higher frequencies (72.5% and 50%, respectively) than in controls (both 2.5%). The occurrence of HCMV infection was rare in both apical lesions (10%) and controls (0%). The presence of EBV DNA in apical lesions was associated significantly with large (> or = 5 mm) lesion size (p = 0.02) but not with symptoms (p = 0.30). Symptomatic manifestation was significantly associated with the co-occurrence (odds ratio [OR], 8.80; 95% confidence interval [CI], 1.69-45.76) but not the sole occurrences of EBNA-2 messenger RNA (OR, 2.29; 95% CI, 0.48-11.06) and large lesion size (OR, 4.02; 95% CI, 0.81-19.89). EBV infection is a frequent event in apical periodontitis, whereas the involvement of HCMV still remains to be elucidated. This study showed that symptomatic manifestation was likely to occur if a large-sized apical periodontitis lesion is aggravated with active EBV infection.
  Journal of endodontics 09/2010; 36(9):1485-9. · 2.95 Impact Factor
• Article: Effects of articaine and ropivacaine on calcium handling and contractility in canine ventricular myocardium

  Norbert Szentandrássy, Adrienn Szabó, János Almássy, István Jóna, Balázs Horváth, Gergely Szabó, Tamás Bányász, Ildikó Márton, Péter P Nánási, János Magyar
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  ABSTRACT: Background and objective: In spite of the widespread clinical use of articaine and ropivacaine there is little information available on the effects of these drugs on myocardial Ca2+ handling. In the present study, therefore, the concentration-dependent effects of articaine and ropivacaine on the components of intracellular Ca2+ handling were studied and compared in canine ventricular myocardium. Methods: Contractility was measured in ventricular trabeculae, [Ca2+]i transients were recorded from electrically stimulated isolated ventricular myocytes loaded with the calcium-sensitive dye fura-2, L-type Ca2+ current was recorded under whole cell patch clamp conditions, and the release and reuptake of Ca2+ was monitored in sarcoplasmic reticulum vesicles. Results: Articaine and ropivacaine caused a reversible and concentration-dependent decrease in amplitude of the [Ca2+]i transient (EC50 = 87.4 ± 12 and 99.3 ± 17 μmol l−1, respectively), which was congruent with the reduction obtained for contractility (EC50 = 73.7 ± 10 and 72.8 ± 14 μmol l−1, respectively). No significant change in diastolic [Ca2+]i was found. L-type Ca2+ current was significantly reduced by articaine and ropivacaine with EC50 values of 327 ± 56 and 263 ± 67 μmol l−1, respectively. Neither Ca2+ release and Ca2+ uptake nor the ATPase activity of the sarcoplasmic reticulum vesicles was altered by articaine or ropivacaine at concentrations less than 200 μmol l−1. In summary, articaine and ropivacaine caused no significant changes at the therapeutically relevant concentrations of the micromolar range. No significant differences between the effects of articaine and ropivacaine on contractility, [Ca2+]i transients, L-type Ca2+ current, and sarcoplasmic reticulum Ca2+ release and uptake were observed. Conclusions: Under conditions of normal application both articaine and ropivacaine are free of cardiodepressant effects; however, a negative inotropic action can be anticipated in cases of accidental intravenous injection or overdose. The observed negative inotropic actions of articaine and ropivacaine are similar in magnitude, and can be mainly attributed to a reduction in net trans-sarcolemmal Ca2+ influx.
  European Journal of Anaesthesiology 01/2010; 27(2):153–161. · 2.23 Impact Factor
• Article: Effects of ropivacaine on action potential configuration and ion currents in isolated canine ventricular cardiomyocytes.

  Adrienn Szabó, Norbert Szentandrássy, Péter Birinyi, Balázs Horváth, Gergely Szabó, Tamás Bányász, Ildikó Márton, János Magyar, Péter P Nánási
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  ABSTRACT: Despite the widespread clinical application of ropivacaine, there is little information on the cellular cardiac effects of the drug. In the current study, therefore, the concentration-dependent effects of ropivacaine on action potential morphology and the underlying ion currents were studied and compared with those of bupivacaine in isolated canine ventricular cardiomyocytes. Action potentials were recorded from the enzymatically dispersed cells using sharp microelectrodes. Conventional patch clamp and action potential voltage clamp arrangements were used to study the effects of ropivacaine on transmembrane ion currents. Ropivacaine induced concentration- and frequency-dependent changes in action potential configuration, including shortening of the action potentials, reduction of their amplitude and maximum velocity of depolarization, suppression of early repolarization, and depression of plateau. Reduction in maximum velocity of depolarization was characterized with an EC50 value of 81 +/- 7 microm at 1 Hz. Qualitatively similar results were obtained with bupivacaine (EC50 = 47 +/- 3 microm). Under voltage clamp conditions, a variety of ion currents were blocked by ropivacaine: L-type calcium current (EC50 = 263 +/- 67 microm), transient outward current (EC50 = 384 +/- 75 microm), inward rectifier potassium current (EC50 = 372 +/- 35 microm), rapid delayed rectifier potassium current (EC50 = 303 +/- 47 microm), and slow delayed rectifier potassium current (EC50 = 106 +/- 18 microm). Ropivacaine, similarly to bupivacaine, can modify cardiac action potentials and the underlying ion currents at concentrations higher than the usual therapeutic range. However, in cases of overdose, cardiac complications may be anticipated both during and after anesthesia due to the blockade of various ion currents.
  Anesthesiology 05/2008; 108(4):693-702. · 5.36 Impact Factor
• Article: Effects of Ropivacaine on Action Potential Configuration and Ion Currents in Isolated Canine Ventricular Cardiomyocytes

  Adrienn Szabó, Norbert Szentandrássy, Péter Birinyi, Balázs Horváth, Gergely Szabó, Tamás Bányász, Ildikó Márton, János Magyar, Péter P. Nánási
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  ABSTRACT: Background: Despite the widespread clinical application of ropivacaine, there is little information on the cellular cardiac effects of the drug. In the current study, therefore, the concentration-dependent effects of ropivacaine on action potential morphology and the underlying ion currents were studied and compared with those of bupivacaine in isolated canine ventricular cardiomyocytes.
  Anesthesiology 03/2008; 108(4):693-702. · 5.36 Impact Factor